Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly charact...Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly characterized by its green abaxial leaf blade,partly connate stipules,and densely patent strigose hairs on stems and potioles.The phylogenetic analysis based on rbc L,nrDNA and rbc L+nrDNA datasets,revealed that all individuals of B.nivea var.strigosa formed a monophyletic group.The conservation status of B.nivea var.strigosa is assessed as“Near Threatened”(NT)according to IUCN evaluation criteria.The discovery of this new variety is not only crucial for the taxonomy of ramie,but also provides reference for the exploration and utilization of ramie.展开更多
With the progress of aging,the incidence of vascular calcification(VC)gradually increases,which is correlated with cardiovascular events and all-cause death,aggravating global clinical burden.Over the past several dec...With the progress of aging,the incidence of vascular calcification(VC)gradually increases,which is correlated with cardiovascular events and all-cause death,aggravating global clinical burden.Over the past several decades,accumulating approaches targeting the underlying pathogenesis of VC have provided some possibilities for the treatment of VC.Unfortunately,none of the current interventions have achieved clinical effectiveness on reversing or curing VC.The purpose of this review is to make a summary of novel perspectives on the interventions of VC and provide reference for clinical decision-making.展开更多
Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic rate.After exposure to environmental stimuli,both the internal reference g...Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic rate.After exposure to environmental stimuli,both the internal reference gene and target gene would be degraded.As a result,it is imperative to consider the accurate capture of nascent RNA and the detection of transcriptional levels of RNA following environmental stimulation.This study aims to create a Click Chemistry method that utilizes its property to capture nascent RNA from total RNA that was stimulated by the environment.Methods The new RNA was labeled with 5-ethyluridine(5-EU)instead of uracil,and the azido-biotin medium ligand was connected to the magnetic sphere using a combination of“Click Chemistry”and magnetic bead screening.Then the new RNA was captured and the transcription rate of 16S rRNA was detected by fluorescence molecular beacon(M.B.)and quantitative reverse transcription PCR(qRT-PCR).Results The bacterial nascent RNA captured by“Click Chemistry”screening can be used as a reverse transcription template to form cDNA.Combined with the fluorescent molecular beacon M.B.1,the synthesis rate of rRNA at 37℃is 1.2 times higher than that at 15℃.The 16S rRNA gene and cspI gene can be detected by fluorescent quantitative PCR,it was found that the measured relative gene expression changes were significantly enhanced at 25℃and 16℃when analyzed with nascent RNA rather than total RNA,enabling accurate detection of RNA transcription rates.Conclusion Compared to other article reported experimental methods that utilize screening magnetic columns,the technical scheme employed in this study is more suitable for bacteria,and the operation steps are simple and easy to implement,making it an effective RNA capture method for researchers.展开更多
This paper reported two newly recorded species, Hypsibius convergens Urbanowicz, 1925 and Hypsibius hypostomus Bartos, 1935, of the genus Hypsibius （Tardigrada; Hypsibiidae） from China. Both species were collected f...This paper reported two newly recorded species, Hypsibius convergens Urbanowicz, 1925 and Hypsibius hypostomus Bartos, 1935, of the genus Hypsibius （Tardigrada; Hypsibiidae） from China. Both species were collected from Taibai Mt, Shaanxi Province. All specimens are deposited at the College of Life Sciences, Shaanxi Normal University, China.展开更多
Five Chinese species of the genus Ectomocoris are revised, keyed and well illustrated by 28 figures. Biarmocoris subgen. n. is established for E. yayeyamae. E. maldonadoi sp. n. is described. E. distanti Cai et Lu is ...Five Chinese species of the genus Ectomocoris are revised, keyed and well illustrated by 28 figures. Biarmocoris subgen. n. is established for E. yayeyamae. E. maldonadoi sp. n. is described. E. distanti Cai et Lu is a new name for E. erebus Distant 1904, junior homonym of E. erebus (Distant, 1903). Lectotypes are designated. E. fiavomaculatus is not represented in China. All the male genitalia are reported for the first time.展开更多
From the perspective of teaching methods theories, this paper probes into the feasibility of adopting the eclectic teaching method in college English teaching, especially the New horizon college English classroom teac...From the perspective of teaching methods theories, this paper probes into the feasibility of adopting the eclectic teaching method in college English teaching, especially the New horizon college English classroom teaching, with the intention of providing new insights for New horizon college English teaching practice.展开更多
本文记述了喙蚤蝇属Trophithauma Schmitz一新种。该属为中国新记录。新种模式标本保存于西北农业大学昆虫博物馆。曲脉喙蚤蝇Trophithauma sinuatum Liu et Chou,新种 雌:体长1.43～1.62毫米。额大部分黄褐色,前缘黄色;单眼三角区隆起...本文记述了喙蚤蝇属Trophithauma Schmitz一新种。该属为中国新记录。新种模式标本保存于西北农业大学昆虫博物馆。曲脉喙蚤蝇Trophithauma sinuatum Liu et Chou,新种 雌:体长1.43～1.62毫米。额大部分黄褐色,前缘黄色;单眼三角区隆起;口上片黄色,向前下方延伸呈舌状;喙高度延长,两条舌内骨带极长。胸黄褐色;中胸侧片下半部及足黄色。翅透明,略呈灰黄色;前缘脉极长,前缘脉与翅长之比为0.78,前缘脉各段比为1.28～1.29:1:1;径分脉(第三纵脉)长而粗,粗于前缘脉,中部向前突出。翅长1.65～1.72毫米,宽0.80～0.82毫米。平衡棒褐色。腹部暗褐色。第2背板大,长度约为第1背板的4倍。第3背板后缘波浪形。第4背板小,高度特化,隐于第3背板之下。第5和第6背板短,两者之间有腺体开口。各背板两侧均无突起。 雄:体长1.1毫米。口上片和喙均不延长。前缘脉与翅长之比为0.64;前缘脉各段比为1.69:1.25:1。生殖器褐色,具细毛;右生殖背板端部分叉;左生殖背板阔;生殖腹板基部圆形,端部分裂成两瓣状。 正模,海南省尖峰岭(1,200米),1992-Ⅳ-4,刘广纯采。副模:1 5,同正模。展开更多
Objective: To investigate the correlation between the microsatellite instability (MSI) and the expression of vascular endothelial growth factor (VEGF) in gastric adenocarcinoma. Methods: PCR SSCP method was used to d...Objective: To investigate the correlation between the microsatellite instability (MSI) and the expression of vascular endothelial growth factor (VEGF) in gastric adenocarcinoma. Methods: PCR SSCP method was used to detect MSI of thirty cases with gastric adenocarcinoma at five loci in each patient. Expression of VEGF was examined by the method of immunohistochemistry. Results: The positive of MSI was in 13 patients out of 30 patients (43.4%) in our study. Positive VEGF Immunostaining was detected in 18 patients (60.0%). VEGF was decreased in microsatellite instability high (MSI H) gastric adenocarcinoma. Conclusion: MSI H and microsatellite stable (MSS) gastric adenocarcinoma may follow a different pathway of angiogenesis. The low frequency of VEGF expression among MSI H cancer may partially explain why these cancers are less aggressive.展开更多
The present paper deals with the subfamily Oxyrhachinae of Membracidae newly recorded from China and the description of a new species. Ozyrhachis sinensis, sp. nov. is very similar to O. tarandus (Fabr.X but differs i...The present paper deals with the subfamily Oxyrhachinae of Membracidae newly recorded from China and the description of a new species. Ozyrhachis sinensis, sp. nov. is very similar to O. tarandus (Fabr.X but differs in adult and nymphal morphological characters and in cytological features.展开更多
From the CHCl3 part of Swertia decora Franch., a traditional medicinal herb of China, two new xanthones, swertiadecoraxanthone-I and swertiadecoraxanthone-II along with four known compounds have been isolated and iden...From the CHCl3 part of Swertia decora Franch., a traditional medicinal herb of China, two new xanthones, swertiadecoraxanthone-I and swertiadecoraxanthone-II along with four known compounds have been isolated and identified on the basis of spectral analysis and chemical evidence.展开更多
文摘Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly characterized by its green abaxial leaf blade,partly connate stipules,and densely patent strigose hairs on stems and potioles.The phylogenetic analysis based on rbc L,nrDNA and rbc L+nrDNA datasets,revealed that all individuals of B.nivea var.strigosa formed a monophyletic group.The conservation status of B.nivea var.strigosa is assessed as“Near Threatened”(NT)according to IUCN evaluation criteria.The discovery of this new variety is not only crucial for the taxonomy of ramie,but also provides reference for the exploration and utilization of ramie.
基金supported by the Peking University Baidu Fund (2019BD019)
文摘With the progress of aging,the incidence of vascular calcification(VC)gradually increases,which is correlated with cardiovascular events and all-cause death,aggravating global clinical burden.Over the past several decades,accumulating approaches targeting the underlying pathogenesis of VC have provided some possibilities for the treatment of VC.Unfortunately,none of the current interventions have achieved clinical effectiveness on reversing or curing VC.The purpose of this review is to make a summary of novel perspectives on the interventions of VC and provide reference for clinical decision-making.
文摘Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic rate.After exposure to environmental stimuli,both the internal reference gene and target gene would be degraded.As a result,it is imperative to consider the accurate capture of nascent RNA and the detection of transcriptional levels of RNA following environmental stimulation.This study aims to create a Click Chemistry method that utilizes its property to capture nascent RNA from total RNA that was stimulated by the environment.Methods The new RNA was labeled with 5-ethyluridine(5-EU)instead of uracil,and the azido-biotin medium ligand was connected to the magnetic sphere using a combination of“Click Chemistry”and magnetic bead screening.Then the new RNA was captured and the transcription rate of 16S rRNA was detected by fluorescence molecular beacon(M.B.)and quantitative reverse transcription PCR(qRT-PCR).Results The bacterial nascent RNA captured by“Click Chemistry”screening can be used as a reverse transcription template to form cDNA.Combined with the fluorescent molecular beacon M.B.1,the synthesis rate of rRNA at 37℃is 1.2 times higher than that at 15℃.The 16S rRNA gene and cspI gene can be detected by fluorescent quantitative PCR,it was found that the measured relative gene expression changes were significantly enhanced at 25℃and 16℃when analyzed with nascent RNA rather than total RNA,enabling accurate detection of RNA transcription rates.Conclusion Compared to other article reported experimental methods that utilize screening magnetic columns,the technical scheme employed in this study is more suitable for bacteria,and the operation steps are simple and easy to implement,making it an effective RNA capture method for researchers.
基金supported by scientific research foundation project of Shaanxi Institute of Education (No.07KJ37Q)
文摘This paper reported two newly recorded species, Hypsibius convergens Urbanowicz, 1925 and Hypsibius hypostomus Bartos, 1935, of the genus Hypsibius （Tardigrada; Hypsibiidae） from China. Both species were collected from Taibai Mt, Shaanxi Province. All specimens are deposited at the College of Life Sciences, Shaanxi Normal University, China.
文摘Five Chinese species of the genus Ectomocoris are revised, keyed and well illustrated by 28 figures. Biarmocoris subgen. n. is established for E. yayeyamae. E. maldonadoi sp. n. is described. E. distanti Cai et Lu is a new name for E. erebus Distant 1904, junior homonym of E. erebus (Distant, 1903). Lectotypes are designated. E. fiavomaculatus is not represented in China. All the male genitalia are reported for the first time.
文摘From the perspective of teaching methods theories, this paper probes into the feasibility of adopting the eclectic teaching method in college English teaching, especially the New horizon college English classroom teaching, with the intention of providing new insights for New horizon college English teaching practice.
文摘本文记述了喙蚤蝇属Trophithauma Schmitz一新种。该属为中国新记录。新种模式标本保存于西北农业大学昆虫博物馆。曲脉喙蚤蝇Trophithauma sinuatum Liu et Chou,新种 雌:体长1.43～1.62毫米。额大部分黄褐色,前缘黄色;单眼三角区隆起;口上片黄色,向前下方延伸呈舌状;喙高度延长,两条舌内骨带极长。胸黄褐色;中胸侧片下半部及足黄色。翅透明,略呈灰黄色;前缘脉极长,前缘脉与翅长之比为0.78,前缘脉各段比为1.28～1.29:1:1;径分脉(第三纵脉)长而粗,粗于前缘脉,中部向前突出。翅长1.65～1.72毫米,宽0.80～0.82毫米。平衡棒褐色。腹部暗褐色。第2背板大,长度约为第1背板的4倍。第3背板后缘波浪形。第4背板小,高度特化,隐于第3背板之下。第5和第6背板短,两者之间有腺体开口。各背板两侧均无突起。 雄:体长1.1毫米。口上片和喙均不延长。前缘脉与翅长之比为0.64;前缘脉各段比为1.69:1.25:1。生殖器褐色,具细毛;右生殖背板端部分叉;左生殖背板阔;生殖腹板基部圆形,端部分裂成两瓣状。 正模,海南省尖峰岭(1,200米),1992-Ⅳ-4,刘广纯采。副模:1 5,同正模。
文摘Objective: To investigate the correlation between the microsatellite instability (MSI) and the expression of vascular endothelial growth factor (VEGF) in gastric adenocarcinoma. Methods: PCR SSCP method was used to detect MSI of thirty cases with gastric adenocarcinoma at five loci in each patient. Expression of VEGF was examined by the method of immunohistochemistry. Results: The positive of MSI was in 13 patients out of 30 patients (43.4%) in our study. Positive VEGF Immunostaining was detected in 18 patients (60.0%). VEGF was decreased in microsatellite instability high (MSI H) gastric adenocarcinoma. Conclusion: MSI H and microsatellite stable (MSS) gastric adenocarcinoma may follow a different pathway of angiogenesis. The low frequency of VEGF expression among MSI H cancer may partially explain why these cancers are less aggressive.
文摘The present paper deals with the subfamily Oxyrhachinae of Membracidae newly recorded from China and the description of a new species. Ozyrhachis sinensis, sp. nov. is very similar to O. tarandus (Fabr.X but differs in adult and nymphal morphological characters and in cytological features.
基金This work was supported in part by Shanghai Municipal Educational Committee (grants S970204).
文摘From the CHCl3 part of Swertia decora Franch., a traditional medicinal herb of China, two new xanthones, swertiadecoraxanthone-I and swertiadecoraxanthone-II along with four known compounds have been isolated and identified on the basis of spectral analysis and chemical evidence.
