As one of the most severe typhoons in the year 2005,Typhoon Longwang is chosen as a case study in this article.Throughout its life,two surveillance flights are carried out on it.Different from previous studies,GPS(glo...As one of the most severe typhoons in the year 2005,Typhoon Longwang is chosen as a case study in this article.Throughout its life,two surveillance flights are carried out on it.Different from previous studies,GPS(global positioning system)Dropwinsonde data collected from the Dropwinsonde Observations for Typhoon Surveillance near the Taiwan Region is chosen to present the thermodynamic and kinetic structure at its two different stages of development.This study suggests that not only kinetic structure but also thermodynamic structure of Longwang are more robust in the second surveillance than the first surveillance,with stronger and larger circulation and a warmer core.Further research shows that the environmental vertical wind shear mainly contributes to the asymmetric structure of the typhoon.The strong vertical wind shear not only results in the distinct asymmetric structure,but also restrains the development of the typhoon.展开更多
Bioinformatics methods for various RNA-seq data analyses are in fast evolution with the improvement of sequencing technologies. However, many challenges still exist in how to efficiently process the RNA-seq data to ob...Bioinformatics methods for various RNA-seq data analyses are in fast evolution with the improvement of sequencing technologies. However, many challenges still exist in how to efficiently process the RNA-seq data to obtain accurate and comprehensive results. Here we reviewed the strategies for improving diverse transcriptomic studies and the annotation of genetic variants based on RNA-seq data. Mapping RNA-seq reads to the genome and transcriptome represent two distinct methods for quantifying the expression of genes/transcripts. Besides the known genes annotated in current databases, many novel genes/transcripts(especially those long noncoding RNAs) still can be identified on the reference genome using RNA-seq. Moreover, owing to the incompleteness of current reference genomes, some novel genes are missing from them. Genome-guided and de novo transcriptome reconstruction are two effective and complementary strategies for identifying those novel genes/transcripts on or beyond the reference genome. In addition, integrating the genes of distinct databases to conduct transcriptomics and genetics studies can improve the results of corresponding analyses.展开更多
基金project of the National Science Foundation of China(40830958,40905020)project of National Basic Research Program of China(2009CB421502)+1 种基金project of the Special Scientific Research for Public Interest(GYHY201006007)project of the Specialized Research Fund for the Doctoral Program of Higher Education of China(20090091120038)
文摘As one of the most severe typhoons in the year 2005,Typhoon Longwang is chosen as a case study in this article.Throughout its life,two surveillance flights are carried out on it.Different from previous studies,GPS(global positioning system)Dropwinsonde data collected from the Dropwinsonde Observations for Typhoon Surveillance near the Taiwan Region is chosen to present the thermodynamic and kinetic structure at its two different stages of development.This study suggests that not only kinetic structure but also thermodynamic structure of Longwang are more robust in the second surveillance than the first surveillance,with stronger and larger circulation and a warmer core.Further research shows that the environmental vertical wind shear mainly contributes to the asymmetric structure of the typhoon.The strong vertical wind shear not only results in the distinct asymmetric structure,but also restrains the development of the typhoon.
基金supported by the National High Technology Research and Development Program of China(2015AA020104)the China Human Proteome Project(2014DFB30010)+1 种基金the National Science Foundation of China(31471239,to Leming Shi)the 111 Project(B13016)
文摘Bioinformatics methods for various RNA-seq data analyses are in fast evolution with the improvement of sequencing technologies. However, many challenges still exist in how to efficiently process the RNA-seq data to obtain accurate and comprehensive results. Here we reviewed the strategies for improving diverse transcriptomic studies and the annotation of genetic variants based on RNA-seq data. Mapping RNA-seq reads to the genome and transcriptome represent two distinct methods for quantifying the expression of genes/transcripts. Besides the known genes annotated in current databases, many novel genes/transcripts(especially those long noncoding RNAs) still can be identified on the reference genome using RNA-seq. Moreover, owing to the incompleteness of current reference genomes, some novel genes are missing from them. Genome-guided and de novo transcriptome reconstruction are two effective and complementary strategies for identifying those novel genes/transcripts on or beyond the reference genome. In addition, integrating the genes of distinct databases to conduct transcriptomics and genetics studies can improve the results of corresponding analyses.
