白血病分型进展

白血病细胞因具有生物学的异质性、分化程度的差异性和细胞形态的多形性等特点,需要明确分型。而它的分型经历了FAB(法国、美国、英国协作组,French-American-BritishCo-operative group)分型、MIC(morphology,immunology and cytogenetic)分型,最后到MICM(morphology,immunology,cytogenetic and molecular)分型。

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i.e., Flounder Gill) from flounder ( Paralichthys olivaceus) gill, SPH (i.e., Sea Perch Heart) from sea perch ( Lateolabrax japonicus ) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream ( Pagrosomus major ) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns was related to morphological changes of fish cells in vitro .

Reliable Analytic Strategy to Correlate the Morphological and Cytological Parameters on Lupinus termis L, against Fusarium oxysporum Infection

Many analytic strategies have emerged to estimate plant responses to Fusarium wilt. The demand for fast and reliable method （diagnosis, prediction） to determine isolate strength accurately is not established yet. Early determination of pathogen strength helps in plant medication. The aim of this study was to develop a faster strategy and method for early determination of fungal isolates strength in correlation to plant response. Till now, the scientists have no consensus on the most correlated parameters that could express wilt precisely. In this study, 30 isolates of Fusarium oxysporum isolated from Lupinus termis L. were used to provide an explicit image about the real strength of Fusarium isolates and its impact on the plant. Wilting percentage ranged from 26.67% to 93.33% of the infected plants depending on isolate virulence. Some of cellular, morphological and physical measurements were conducted on 8 out of 30 isolates, including root （length, fresh weight （FW） and dry weight （DW））, nodules （water content （WC）, FW, DW）, stem （height, WC, FW, DW）, total leaves/plant （WC, FW, DW） and the fourth leaf （WC, FW, DW, leaf area, epidermal cell area, epidermal cell number, succulence）. Hierarchical clustering was used to determine the variance between the isolates. Detrended correspondence analysis （DCA） and canonical correspondence analysis （CCA） were used to determine the most important growth parameters that could express wilting accurately. The CCA results showed that most of the measured parameters on the fourth leaf, except for leaf epidermal cell number, were highly and positively correlated to wilt. That makes these specific parameters valuable and sensitive for any changes in isolates strength. Accordingly, a mathematical model was created to be helpful in the quick determination of isolate strength and precise medication.

AGING OF HUMAN MATURE ERYTHROCYTES IS LIKE A PROCESS OF APOPTOSIS IN ENUCLEATED CELL

Apoptosis of nucleated cells is well known, but how about the unnucleated cells is still not elucidated. In the present paper, the morphological and biochemical features of the aged erythrocytes were observed and compared with the characteristic events of apoptosis. Membrane of aged erythrocytes tends to shrink, protrude, from vesicle and lose lipid asymmetry. Aged erythrocytes were removed by phagocytosis. Both of the events are very similar to the apoptotic nucleated cells. The authors suggested that aging of erythro- cytes is also a process of apoptosis.

婴儿急性白血病30例

目的探讨婴儿急性白血病（IAL）的临床表现、细胞遗传学及分子生物学检查特点。方法对2005年1月-2009年12月华中科技大学同济医学院附属同济医院和武汉市儿童医院初诊的30例IAL患儿临床表现、实验室检查、细胞形态学、免疫学、细胞遗传学和分子遗传学分型检查及随访资料进行回顾性分析。结果临床表现为发热14例,皮下出血5例,面色苍白、面色苍黄各4例。全身浅表淋巴结大5例,骨质破坏5例,肝和（或）脾超过肋下4 cm 17例,中枢神经系统浸润1例。发病至就诊时间2~75 d。WBC计数为114.78（1.06~681.37）×109L-1,Hb 67（38~101）g.L-1,PLT 61（1~355）×109L-1。IAL 30例外周血涂片均可见幼稚细胞。30例中ALL 15例,急性非淋巴细胞白血病（ANLL）14例,分类不明1例。12例行免疫分型检查,5例ALL均为B系,其中CD10表达4例,CD7与CD15共同表达1例;7例ANLL中CD19表达2例。12例染色体检查结果异常者3例。13例IAL行融合基因检测,结果E2A/PBX1、BCR/ABL阳性各1例。30例中仅4例ALL接受化疗,但最终均放弃治疗。而对未治疗的26例1个月后电话随访均死亡。结论 IAL的临床表现缺乏特异性;ALL及ANLL均可发生,且比率相当;ALL中以B系多见;IAL常伴染色体异常。但由于绝大多数未得到有效化疗而放弃治疗,故IAL的预后较年长儿差。

Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide

Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investi- gation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide （H2O2）. Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography （HPLC） analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical scavenging activity indicate that oat peptide-dch extract has a direct and concentration-dependent antioxidant activity. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling （TUNEL） assay for apoptosis showed that administration of H2O2 in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H2O2, but ap- plication oat peptides with H2O2 at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H2O2-induced decrease of superoxide dismutase （SOD） and the inhibition of malondialdehyde （MDA）. The results demonstrate that oat peptides possess antioxidant activity and are effective against H2O2-induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury.

Morphogenesis of T-tubules in heart cells: the role of junctophilin-2

The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not exist in neonatal cardiomyocytes. In the present study, we quantified the morphological changes in TTs during heart cell maturation and investigated the role of junctophilin-2 (JP2), a protein known to anchor the sarcoplasmic reticulum (SR) to TT, in changes to TT morphological parameters. Analysis of confocal images showed that the transverse elements of TTs increased, while longitudinal elements decreased during the maturation of TTs. Fourier transform analysis showed that the power of ~2 m spatial components increased with cardiomyocytes maturation. These changes were preceded by increased expression of JP2, and were reversed by JP2 knockdown. These findings indicate that JP2 is required for the morphogenesis of TTs during heart development.