用细胞化学法,直接涂片测定人周围血 T 淋巴细胞中的酸性α—醋酸萘酯酶(ANAE),从而达到检测 T 淋巴细胞的目的,籍以作为反映机体细胞免疫功能的一种指标。58例正常健康人的周围血 T 淋巴细胞为52—87%,平均值±S.D73.3±9.8%;...用细胞化学法,直接涂片测定人周围血 T 淋巴细胞中的酸性α—醋酸萘酯酶(ANAE),从而达到检测 T 淋巴细胞的目的,籍以作为反映机体细胞免疫功能的一种指标。58例正常健康人的周围血 T 淋巴细胞为52—87%,平均值±S.D73.3±9.8%;248例癌瘤患者为20—85%,平均值±S.D54.6±12.2%,明显低于正常人,二者相比 t 值为24.24,P<0.01。总之,利用周围血液涂片,以细胞化学法介 ANAE(+)识别 T 细胞,可作临床上反应病人机体细胞免疫功能的一种简单易行的指标。展开更多
The effect were examined in vitro of the ionic composition of the incubation medium on the acquisition and loss of the potential for motility were exammined by the spermatozoa of the Japanese eel （Anguilla japonica）...The effect were examined in vitro of the ionic composition of the incubation medium on the acquisition and loss of the potential for motility were exammined by the spermatozoa of the Japanese eel （Anguilla japonica）. Milt was obtained from male eels that had been artificially matured by repeated injections of HCG. The percent motility increased with increasing concentrations of Na+、K+、Ca2+ and Mg2+ ions (0-400mmol·L-1) and decreased with concentrations of those ions(500-1000 mmol·L-1), eel sperm was collected and then incubated with nitric oxide donor-sodium nitroprusside in different doses. Eel sperm motility was accelerated or maintained at low dose of sodium nitroprusside （SNP） (10-10-10-6 mol·L-1), while marked inhibition effects were found at high dose (SNP>10-5mol·L-1). Nitric oxide affects the motility of eel sperm in dose-dependent manner. The eel sperm of maturation by SPN and HCG was used for fertilizing three batches of eggs and hatchability is higher than the sperm of maturation by HCG.展开更多
文摘用细胞化学法,直接涂片测定人周围血 T 淋巴细胞中的酸性α—醋酸萘酯酶(ANAE),从而达到检测 T 淋巴细胞的目的,籍以作为反映机体细胞免疫功能的一种指标。58例正常健康人的周围血 T 淋巴细胞为52—87%,平均值±S.D73.3±9.8%;248例癌瘤患者为20—85%,平均值±S.D54.6±12.2%,明显低于正常人,二者相比 t 值为24.24,P<0.01。总之,利用周围血液涂片,以细胞化学法介 ANAE(+)识别 T 细胞,可作临床上反应病人机体细胞免疫功能的一种简单易行的指标。
文摘The effect were examined in vitro of the ionic composition of the incubation medium on the acquisition and loss of the potential for motility were exammined by the spermatozoa of the Japanese eel （Anguilla japonica）. Milt was obtained from male eels that had been artificially matured by repeated injections of HCG. The percent motility increased with increasing concentrations of Na+、K+、Ca2+ and Mg2+ ions (0-400mmol·L-1) and decreased with concentrations of those ions(500-1000 mmol·L-1), eel sperm was collected and then incubated with nitric oxide donor-sodium nitroprusside in different doses. Eel sperm motility was accelerated or maintained at low dose of sodium nitroprusside （SNP） (10-10-10-6 mol·L-1), while marked inhibition effects were found at high dose (SNP>10-5mol·L-1). Nitric oxide affects the motility of eel sperm in dose-dependent manner. The eel sperm of maturation by SPN and HCG was used for fertilizing three batches of eggs and hatchability is higher than the sperm of maturation by HCG.
