A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related t...A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesis in both cell lines. These results indicated that C_ 20hi cells grew faster than C_ 20D cells, and average ajmalicine content in C_ 20hi cells was 18.4 times more than that in C_ 20D when cultured in the production medium. In the growth medium, average ajmalicine content in C_ 20hi cells was 31.9 times more than that in C_ 20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C_ 20hi and C_ 20D cells indicated that tryptophan decarboxylase (TDC), strictosidine synthase (SSS) and geraniol_10_dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C_ 20hi cells are relatively stable in five years of culture.展开更多
In the paper, necessary and sufficient conditions are provided for a function involving the divided difference of two psi functions to be completely monotonic. Consequently, a class of inequalities for sums are presen...In the paper, necessary and sufficient conditions are provided for a function involving the divided difference of two psi functions to be completely monotonic. Consequently, a class of inequalities for sums are presented, the logarithmically complete monotonicity of a function involving the ratio of two gamma functions are derived, and two double inequalities for bounding the ratio of two gamma functions are discovered.展开更多
The authors obtain various versions of the Omori-Yau＇s maximum principle on complete properly immersed-submanifolds with controlled mean curvature in certain product manifolds, in complete Riemannian manifolds whose ...The authors obtain various versions of the Omori-Yau＇s maximum principle on complete properly immersed-submanifolds with controlled mean curvature in certain product manifolds, in complete Riemannian manifolds whose k-Ricci curvature has strong quadratic decay, and also obtain a maximum principle for mean curvature flow of complete manifolds with bounded mean curvature. Using the generalized maximum principle, an estimate on the mean curvature of properly immersed submanifolds with bounded projection in N1 in the product manifold N1 x N2 is given. Other applications of the generalized maximum principle are also given.展开更多
文摘A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesis in both cell lines. These results indicated that C_ 20hi cells grew faster than C_ 20D cells, and average ajmalicine content in C_ 20hi cells was 18.4 times more than that in C_ 20D when cultured in the production medium. In the growth medium, average ajmalicine content in C_ 20hi cells was 31.9 times more than that in C_ 20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C_ 20hi and C_ 20D cells indicated that tryptophan decarboxylase (TDC), strictosidine synthase (SSS) and geraniol_10_dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C_ 20hi cells are relatively stable in five years of culture.
基金supported partially by the China Scholarship Council and the Science Foundation of Tianjin Polytechnic Universitysupported in part by the Natural Science Foundation Project of Chongqing,China(Grant No.CSTC2011JJA00024)+1 种基金the Research Project of Science and Technology of Chongqing Education Commission,China(Grant No.KJ120625)the Fund of Chongqing Normal University,China(Grant Nos.10XLR017 and 2011XLZ07)
文摘In the paper, necessary and sufficient conditions are provided for a function involving the divided difference of two psi functions to be completely monotonic. Consequently, a class of inequalities for sums are presented, the logarithmically complete monotonicity of a function involving the ratio of two gamma functions are derived, and two double inequalities for bounding the ratio of two gamma functions are discovered.
基金supported by the National Natural Science Foundation of China (No. 10971028)
文摘The authors obtain various versions of the Omori-Yau＇s maximum principle on complete properly immersed-submanifolds with controlled mean curvature in certain product manifolds, in complete Riemannian manifolds whose k-Ricci curvature has strong quadratic decay, and also obtain a maximum principle for mean curvature flow of complete manifolds with bounded mean curvature. Using the generalized maximum principle, an estimate on the mean curvature of properly immersed submanifolds with bounded projection in N1 in the product manifold N1 x N2 is given. Other applications of the generalized maximum principle are also given.
