如何确定变更处分的起始时间和解除期限

一段时间以来,一些地区和部门询问,对国家公务员的行政处分予以变更后,如何确定其变更处分的起始时间和解除期限,并希望予以明确。现将人事部人发〔2000〕57号《关于变更国家公务员行政处分后有关问题的通知》刊发,以作答复。

深基坑工程动态施工反演分析与变形预报

提出了动态施工反演分析的思想，即在常规的反分析过程中引入逐步开挖和逐道支撑的动态施工因素，以求仿真模拟工程实际情况，进而为相继施工阶段的变形预报提供可靠保证。文中基于任意施工阶段间围护墙体变形与内支撑梁轴力的增量测值和施工ＦＥＭ优化反演分析法，反推了各土层的弹性模量系数，给出了作用在墙体两侧的土压力分布，并预报各相继施工阶段的墙体和土体变形、内力及内支撑梁轴力。

对元祐更化应重新评价

王安石变法,在北宋掀起了一场轩然大波,元祐更化则是这次变法的终结。长期以来,人们对王安石变法褒贬不一,但一般肯定变法者则否定元祐更化,否定变法者则肯定元祐更化,二者必居其一,把这个问题绝对化了。 目前史学界大多认为王安石变法具有进步意义,而把元祐更化看作是一场复辟和历史倒退。

A stepwise camera calibration method incorporating compensation for eccentricity error

In visual measurement,high-precision camera calibration often employs circular targets.To address issues in mainstream methods,such as the eccentricity error of the circle from using the circle’s center for calibration,overfitting or local minimum from fullparameter optimization,and calibration errors due to neglecting the center of distortion,a stepwise camera calibration method incorporating compensation for eccentricity error was proposed to enhance monocular camera calibration precision.Initially,the multiimage distortion correction method calculated the common center of distortion and coefficients,improving precision,stability,and efficiency compared to single-image distortion correction methods.Subsequently,the projection point of the circle’s center was compared with the center of the contour’s projection to iteratively correct the eccentricity error,leading to more precise and stable calibration.Finally,nonlinear optimization refined the calibration parameters to minimize reprojection error and boosts precision.These processes achieved stepwise camera calibration,which enhanced robustness.In addition,the module comparison experiment showed that both the eccentricity error compensation and the camera parameter optimization could improve the calibration precision,but the latter had a greater impact.The combined use of the two methods further improved the precision and stability.Simulations and experiments confirmed that the proposed method achieved high precision,stability,and robustness,suitable for high-precision visual measurements.

Aphid-resistant Transgenic Tobacco Plants Expressing Modified gna Gene

A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco (Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.

社会因素与语义标记对立的变异

语言中的标记现象极为普遍,任何一种语言中都有着不同程度,不同层次的标记现象存在。标记现象既是语言本身所固有的特征,也是社会生活与文化因素的产物。从语言的语音和语法系统方面看,标记现象是以某种较为规律和极少变异的形态出现;

Site-directed Mutagenesis of Streptomyces avermitilis aveD Gene

[Objective] The aim of this study was to produce Streptomyces avermitilis strain with site-directed mutagenesis in aveD gene,and so as to provide theoretical basis for genetic breeding of S.avermitilis.[Method] PCR-driven overlap extension was conducted for the site-directed mutagenesis in aveD gene;the mutated aveD gene then was used to construct vector pDC3（pKC1139∷aveD） via molecular manipulations like in vitro enzyme digestion and ligation;the vector pDC3（pKC1139∷aveD） was then introduced to aveD deletion mutant 489 of avermectin-producing strain S.avermitilis 76-9.[Result] Mutant strain 536 of site-directed mutagenesis of S.avermitilis 76-9 was obtained by homologous recombination.The sequencing results show that the sixty-ninth base C in aveD-coding region of mutant 536 was substituted by T,and the corresponding amino acid Thr was mutated to be Ile.[Conclusion] This study laid basis for the development of strains specifically producing avermectin B.

A Preliminary Study on Genetic Variation of g E Gene of an Epidemic Pseudorabies Virus Strain and Its Pathogenicity to Piglets

[Objective] This study aimed to investigate the genetic variation of g E gene of an epidemic pseudorabies virus（PRV） strain and its pathogenicity to piglets. [Method] By serial passage in Vero cells, a PRV strain was isolated from the brain tissues of stillborn fetuses delivered by sows with suspected PRV infection and preliminarily identified by PCR. g E gene of the isolated PRV strain was amplified and sequenced for phylogenetic analysis. In addition, the pathogenicity of the isolated PRV strain to 6-week-old piglets was evaluated. [Result] A PRV strain was successfully isolated and named PRV N5 B strain, which could proliferate in Vero cells and TCID50 of the 15 thgeneration virus liquid reached 10^7.125/0.1 ml. Specific bands could be amplified by PCR. g E gene in the isolated PRV strain was 1 740 bp in length. A phylogenetic tree was constructed based on full-length g E sequences, which showed that PRV N5 B strain and PRV strains isolated since 2012 were clustered into the same independent category and shared 99.7%-100% homology of nucleotide sequences. Compared with related sequences published previously, there were insertions of three consecutive bases at two loci. Animal experiments showed that intranasal inoculation of 6-week-old piglets with 2 ml of PRV N5 B strain（10^6/0.1 ml） led to a mortality rate of 100%. [Conclusion] In this study,genetic variability of g E gene in PRV N5 B isolate and its pathogenicity to piglets were analyzed, which provided a theoretical basis for the development of new vaccines to prevent and control porcine pseudorabies.

A CMOS Fully Integrated Frequency Synthesizer with Stability Compensation

A complete closed-loop third order s-domain model is analyzed for a frequency synthesizer. Based on the model and root-locus technique, the procedure for parameters design is described, and the relationship between the process,voltage,and temperature variation of parameters and the loop stability is quantitatively analyzed. A variation margin is proposed for stability compensation. Furthermore,a simple adjustable current cell in the charge pump is proposed for additional stability compensation and a novel VCO with linear gain is adopted to limit the total variation. A fully integrated frequency synthesizer from 1 to 1.05GHz with 250kHz channel resolution is implemented to verify the methods.

流化床机组汽机运行节能降耗措施分析

我国现在使用的300 MW汽轮机组大部分都是20世纪80年代之前引进的国外技术,较为落后。由于汽轮机的系统原理、结构设计、安装维护等各方面的原因,大都存在着不同程度的问题,经济性差,运行能耗高,节能降耗空间广阔。

Site-directed Mutagenesis Based on Overlap Extension PCR

[Objective] To establish an efficient, convenient and economical method for site-directed mutagenesis. [Method] The target mutation was introduced into primers designed by DNAMAN5.0 software. Through overlap extension PCR for twice obtained the mutation gene which of the full length of the recombinant Human Tissue type plasminogen activator （Reteplase）. The mutation gene cloned it into pEASY- blunt simple cloning vector for sequencing. [Result] The sequencing results showed that three site mutations were fully consistent with the expected results （10~ site had been added a base-pair of A, C had been changed into G at 137~ site, G had been changed into A at 686~ site）.Three site mutations were introduced by using overlap extension PCR on one-step. The overall rate of obtaining the mutant sites was 100%. Site-directed mutagenesis will clone the recombinant Human Tissue type plas- minogen activator and laid the basis for the functional study. [Conclusion] Site-directed mutagenesis was successfully implemented based on the overlap extension PCR which is an efficient, convenient and economical DNA-directed mutagenesis method.

Interfacial morphology evolution in directionally solidified Al-1.5%Cu alloy

The solid-liquid interracial morphology evolution was investigated in directional solidification （DS） of Al-1.5%Cu alloy （mass fraction）. The results show that the solidified microstructural evolution is gradual other than sharp, and the microstructure patterns are interesting and diversiform at the pulling rate ranging from 30 μm/s to 1500 μm/s. Indeed, dendrite to cell transition follows this sequence： dendrites→→banded cellular dendrites→elongated cells and part of dendrites→main elongated cells and little dendrites. Moreover, the present microstructure is not normal microstructure as we saw before. Further, according to the experimental phenomenon, the dendrite to cell transition was studied theoretically. Dendrite tip shape is an important parameter to characterize the dendrite to cell transition. As the dendrite to cell transition is far from equilibrium solidification, non-equilibrium solidification is taken into consideration in calculation. Finally, it is speculated that the dendrite to cell transition would occur at the minimum tip radius.

A Simple and Easy Method for Site-specific Mutagenesis Using Long-distance Inverse PCR in the Presence of Pfu-DNA Polymerase

Site_specific mutagenesis has been widely used in molecular biology and biochemistry. The authors have developed a simple and easy method for site_specific mutagenesis of any genes on plasmids using long distance inverse PCR in the presence of Pfu_DNA polymerase. The efficiency of this method is higher than 90% and the entire procedure can be performed just in one tube. No subcloning is needed. This method is especially useful for obtaining mutant genes on large plasmids such as Ti plasmids used for plant transformation.

Physiological Character and Gene Mapping in a New Green-revertible Albino Mutant in Rice

A green-revertible albino mutant-Qiufeng M was found from the japonica rice （Oryza sativa L. ssp. japonica） Qiufeng in the field. The first three leaves of the mutant were albino with some green. The leaf color became pale green since the fourth leaf and the glume had the same phenomenon as the first three leaves. The measuring data of the pigment content confirmed the visually observed results. It truly had a remarkable changing process in the leaf color in Qiufeng M. Comparison of the main agronomic characters between Qiufeng and Qiufeng M indicated that the neck length and grain weight showed significant difference at the 1% level, and other characters were not different. Genetic analysis showed that the green-revertible albino trait was controlled by a single recessive nucleic gene. Using 209 recessive mutant individuals in the F2 population derived from the cross Pei＇ai 64S × Qiufeng M, a gene, tentatively named gra（t）, was located between the SSR markers of RM475 and RM2-22 on the long arm of chromosome 2. The genetic distance were 17.3 cM and 2.9 cM respectively.

Deformation caused by the 2011 eastern Japan great earthquake monitored using the GPS single-epoch precise point positioning technique

Crustal deformation can provide constraints for studying earthquake rupture and shock wave transmission for the Mw9.0 eastern Japan great earthquake. Using the single- epoch precise point positioning （PPP） method and the appropriate positioning flow, we process GPS data from six IGS （International GNSS Service） sites （e.g., MIZU, TSK2, USUD, MTKA, AIRA and KSMV） located in Japan and obtain the positioning results with centimeter scale precision. The displacement time series of the six sites are analyzed using the least squares spectral analysis method to estimate deformations caused by the Mw9.0 mainshock and the Mw7.9 aftershock, and the cumulative displacements after 1 day. Mainshock displacements at station MIZU, the nearest site to the mainshock in the North （N）, East （E）, and Up （U） directions, are -1.202 m, 2.180 m and -0.104 m, respectively, and the cumulative deformations after 1 day are -1.117 m, 2.071 m and -0.072 m, respectively. The displacements at station KSMV, the nearest site to the Mw7.9 aftershock in the N, E and U directions, are -0.032 m, 0.742 m and -0.345 m, respectively. The other sites obviously experienced eastern movements and subsidence. The deformation vectors indicate that the horizontal displacements caused by the earthquake point to the epicenter and rupture. Elastic bounds evidently took place at all sites. The results indicate that the crustal movements and earthquake were part of a megathrust caused by the Pacific Plate sinking under the North American Plate to the northeast of Japan island arc.

某大型商业建筑暖通空调节能优化设计

本文着重介绍了一个大型的商业建筑的暖通空调节能设计。本项目采用了定变结合的冷源配置方式,热源采用热回收型真空热水锅炉,空调系统采用了分层空调和普通的散流器送风相结合,通风系统也综合考虑人防、建筑等因素,合理设计排风再利用,水系统采用分区两管制,兼顾节能和运行调节的方便性。

New criterion for delay-dependent absolute stability of Lurie system with interval time-varying delay

The delay-dependent absolute stability for a class of Lurie systems with interval time-varying delay is studied. By employing an augmented Lyapunov functional and combining a free-weighting matrix approach and the reciprocal convex technique, an improved stability condition is derived in terms of linear matrix inequalities （LMIs）. By retaining some useful terms that are usually ignored in the derivative of the Lyapunov function, the proposed sufficient condition depends not only on the lower and upper bounds of both the delay and its derivative, but it also depends on their differences, which has wider application fields than those of present results. Moreover, a new type of equality expression is developed to handle the sector bounds of the nonlinear function, which achieves fewer LMIs in the derived condition, compared with those based on the convex representation. Therefore, the proposed method is less conservative than the existing ones. Simulation examples are given to demonstrate the validity of the approach.

Studies on Ultra-dry Storage and Genetic Stability of Vegetable Seeds

[Objective] To study the long-term ultra-dry storage method and genetic stability of vegetable seeds.[Method] Seeds of Lycopersicum esculentum,Raphanus satuvus and Apium graveolen.were chosen as material.The changes of seed vigor,viability and genetic stability after ultra-storage were discussed by studying the seed potentiality,shoot length,germination percentage and the chromosome aberration rate of root tip cells.[Results] Maintaining the low moisture content,different vegetable species had different storage effects of the long-term storage seeds under normal temperature.The Lycopersicum esculentum and Raphanus satuvus seeds were more suitable to ultra-dry storage at normal temperature,and could keep good genetic stability,while the seeds of Apium graveolen had bad performance.[Conclusion] This study established the foundation of studying ultra-dry storage of vegetable seeds.

ROBUST STABILIZATING FOR A OLASS OF UNOERTAIN NONLINEAR INTEROONNECTED SYSTEMS USING VARIABLE STRUCTURE CONTROL

The robust stabilizating control problem for a class of uncertain nonlinear large-scale systems is discussed. Based on the theory of both input/output (I/O) linearization and decentralized variable structure control (VSC),two-level and decentralized variable structure control laws for this kind of systems are presented respectively,which achieve asymptotically stabilization despite the uncertainties and disturbances. At last,sirnulation of the disturbed two-pendulum system is given to illustrate the feasibility of proposed technique.

化学计算教学探索

化学计算是中学化学教学中的重点、又是难点,本文介绍的“分定变”教学法,有效地解决了这一难题,在教学实践中获得了较好的效果.