琵琶定品与音高关系

20世纪50年代,琵琶演奏者们为了适应民族和西洋管弦乐队的需要,将原来琵琶的非半音定品,改为半音定品,并确定了六相二十四品(或二十五品)的形制。由于琵琶音域宽广、转调自如,因此许多人认为它是十二平均律乐器。但是,通过对六相二十四(五)品、南音四相十品等多种多张琵琶定品与音高测算的分析,对琵琶演奏者、制作者的采访,结合琵琶定品与古琴徽位的比较研究,本文认为:琵琶是以半音定品,但半音定品不等于十二平均律;琵琶定品,既有纯律因素,又有十二平均律倾向,还有五度相生律遗留。

AFLP Fingerprint and SCAR Marker of Watermelon Core Collection

The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of watermelon ( Citrullus lanatus (Thunb.) Mansf.), but their effectiveness was limited due to the extremely narrow genetic background among watermelon genotypes. In this research, amplified fragment length polymorphism (AFLP), which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct genetic analysis and variety identification of thirty genotypes of watermelon core collection that represent a wide range of breeding and commercially available germplasm. As a result, a DNA fingerprint based on 15 bands amplified with four primer combinations was developed. In this fingerprint, each genotype has its unique fingerprint pattern and can be distinguished from each other. Furthermore, in or der to facilitate the utilization of AFLP marker in practice, one specific AFLP band of genotype 'PI296341' coming from fragment amplified by primer combination E-AT/M-CAT was successfully converted into a sequence characterized amplified region (SCAR) marker.

Resistance of Major and Backup Rice Cultivars against Ustilaginoidea virens in Anhui Province

[Objective] The aim was to study resistance of major and backup rice cul- tivars against Ustilaginoidea virens. [Method] Experiments and surveys were made on resistance of seventeen backup rice cultivars and some major cultivars in Anhui Province to identify resistance of different rice cultivars. [Result] Yanjing No.9, 80You 226, Tianxie No.l, A01 Xian, Lvjingnuo No.6 were moderate resistant; A03 Xian and Yangjing 636 were susceptible and the rest ten showed moderate susceptibility. Based on surveys on major rice cultivars, most of Liangyou rice series are suscept- able and novel Liangyou Xiang 4 enjoys resistance to certain extent. [Conclusion] The research provided references for resistance of rice against diseases in Anhui Province.

Study and Identification of Gaultheria yunnanensis Leaves from Different Regions

[Objective] In order to identify and comparatively study leaves of ethnic medicine Gaultheria yunnanensis from different regions of Guizhou.[Method] Characteristics,microscopic,physical and chemical identification were studied on Gaultheria yunnanensis.[Result] There was no obvious difference in characteristics and microscopic sections among Gaultheria yunnanensis leaves from different regions.Blue fluorescence spots were observed clearly by TLC（Thin Layer Chromatography） test.[Conclusion]The study provided a basis for improvement of the quality standard of the Gaultheria yunnanensis.

QTL Mapping and Q×E Interactions of Grain Cooking and Nutrient Qualities in Rice Under Upland and Lowland Environments

Grain cooking and nutrient qualities are the most important components of rice （Oryza sativa L.） quality. A doubled haploid （DH） population from a cross between two japonica cultivars was used to examine the phenotypic values and potential QTLs for the quality traits. The cooking and nutrient quality traits, including the amylose content （AC）, the gel consistency （CJC）, the gelatinization temperature （GT）, and the protein content （PC）, in rice grown under upland and lowland environments were evaluated. Significant differences for AC, GC, GT, and PC between upland and lowland environments were detected. The phenotypic values of all four traits were higher under upland environment than lowland environment. The value of PC under upland environment was significantly higher （by 37.9%） than that under lowland environment. This suggests that upland cultivation had large effect on both cooking and nutrient qualifies. A total of seven QTLs and twelve pairs of QTLs were detected to have significant additive and epistatic effects for the four traits. Significant Q x E interaction effects of two QTLs and two pairs of QTLs were also discovered. The general contribution of additive QTLs ranged from 1.91% to 19.77%. The Q × E interactions of QTLs QGt3 and QAc6 accounted for 8.99% and 47.86% of the phenotypic variation, respectively, whereas those of the 2 pairs of epistatic QTLs, QAc6-QAcllb and QAc8-QAc9, accounted for 32.54% and 11.82%, respectively. Five QTLs QGt6b, QGt8, QGt11, QGcl, and QPc2, which had relatively high general contribution and no Q x E interactions, were selected to facilitate the upland rice grain quality breeding.

Comparison of Several Statistical Analysis Models for Genotypic Stability of Saccharum officinarum

[Objective] The study aimed to compare several statistical analysis models for estimating the sugarcane （Saccharum spp.） genotypic stability. [Method] The data of sugarcane regional trials in Guangdong, in 2009 was analyzed by three models respectively： Finlay and Wilkinson model： the additive main effects and multiplicative interaction （AMMI） model and linear regression-principal components analysis （LR- PCA） model, so as to compare the models. [Result] The Finlay and Wilkinson model was easier, but the analysis of the other two models was more comprehensive, and there was a bit difference between the additive main effects and multiplicative inter- action （AMMI） model and linear regression-principal components analysis （LR-PCA） model. [Conclusion] In practice, while the proper statistical method was usually con- sidered according to the different data, it should be also considered that the same data should be analyzed with different statistical methods in order to get a more reasonable result by comparison.

Effect of Main Cultivation Factors on Oil Quality and Stability of Brassica napus Hybrids

[Objective] This study aimed to analyze the influence of five cultivation factors： sowing date, plant density, nitrogen fertilizer amount, phosphate fertilizer amount and potash fertilizer amount on oil quality and stability of Brassica napus hybrids. [Method] Statistical analysis was carried out based on the data from previous studies and our work. [Result] Sowing date had no significant influence on oil quality and stability of B. napus hybrids. The plant density shared a positive correlation with 18-C fatty acid （FA） content, unsaturated fatty acid （UFA） content, total content of linoleic acid and linolenic acid, index of unsaturated fatty acid （IUFA）, so a large plant density can improve the oil quality （by increasing18-C FA content, UFA content, total content of linoleic acid and linolenic acid）, but reduce the oil stability （by increasing IUFA）. The amount of nitrogen fertilizer had no significant influence on the contents of 18-C FA and UFA, but significant positive correlations with the total content of linoleic acid and linolenic acid and IUFA. So, the total content of linoleic acid and linolenic acid can be reduced by increasing nitrogen amount, meanwhile the oil stability was also decreased. Phosphate fertilizer amount did not affect 18-C FA significantly, but was negatively correlated with the UFA content, total content of linoleic acid and linolenic acid, and IUFA. So, increasing the amount of applied phosphate fertilizer can reduce UFA content, total content of linoleic acid and linolenic acid, thereby improving oil stability. Potash fertilizer had no significant influence on the IUFA, a significant positive correlation with 18-C FA content, an insignificant positive correlation with the the UFA content, and a negative correlation with the total content of linoleic acid and linolenic acid. The oil content of rapeseed shared an extremely significant positive correlation with 18-C FA content and UFA content, an negative correlation with the total content of linoleic acid and linolenic acid, and no significant correlation with IUFA. [Conclusion] This study identified the effects of five main cultivation factors on oil quality and stability of B. napus hybrids, and the correlations of oil content with 18-C FA content, UFA content, total content of linoleic acid and linolenic acid, and IUFA.

Qualitative and Quantitative Analysis of Rhizoma of Polygonum cuspidatum

Aim To establish reliable methods for evaluating the quality of rhizoma of Polygonum cuspidatum( Huzhang in Chinese). Methods TLC and HPLC were employed for the chemical identification and content determination,respectively. Results A qualitative TLC method and a quantitative HPLC method with piceid as the reference substance were established, respectively. With piceid as the reference substance and ethyl acetate-methanol-formic acid-water ( 19:3:0.5:1) as the mobile phase, a TLC method for the identification of Huzhang from the commonly used crude drugs of the same family was also set up. Conclusion The established TLC method can reasonably appraise the quality of the drug and easily distinguish Huzhang from the other commonly used crude drugs of the same family. The HPLC method for determining piceid is simple, reproducible, accurate, and feasible.

Configuration knowledge modeling of customizable products based on semantic web technologies

In order to solve the problem of modeling product configuration knowledge at the semantic level to successfully implement the mass customization strategy, an approach of ontology-based configuration knowledge modeling, combining semantic web technologies, was proposed. A general configuration ontology was developed to provide a common concept structure for modeling configuration knowledge and rules of specific product domains. The OWL web ontology language and semantic web rule language （SWRL） were used to formally represent the configuration ontology, domain configuration knowledge and rules to enhance the consistency, maintainability and reusability of all the configuration knowledge. The configuration knowledge modeling of a customizable personal computer family shows that the approach can provide explicit, computerunderstandable knowledge semantics for specific product configuration domains and can efficiently support automatic configuration tasks of complex products.

Identification of Faba Bean(Vicia faba L.)Germplasm Resources in Hubei Province and Evaluation on Their Main Agronomic Traits

Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank of Hubei Province were evaluated, moreover, the identification on the disease resistance and the quality was carried out on parts of germplasm resources. The results showed that the faba beangermplasm resources in Hubei Province were abundant, the variation range of agronomic traits like the growth period, plant height and 100-bean weight, etc., was great, and the range of choice was wide. In the aspect of quality, there were 9 germplasms withthe content of crude protein higher than 30%, and the variation range of amylose content in total starch was large, which provided excellent sources of parents for breedinq hi.qh-quality faba bean varieties in Hubei Province.

Purity Identification of Xinshikui 6 Using SSR Marker Technique

[Objective] SSR molecular marker technique was used to determine the purity of sunflower seed with the aim to provide accurate, convenient method for the identification of the purity of hybrid seeds in production and processing. [Method] With the DNA of Xinshikui 6 and its parents as template, about 100 pairs of SSR molecular markers were screened after DNA extraction, PCR amplification and electrophoresis production. [Results] SSR polymorphic primer marker 532 produced a specific band of 469 bp in the female parent, and a specific band of 451 bp in the male parent; primer marker 574 produced a specific band of 364 bp in the female parent, and a specific band of 384 bp in the male parent. The indoor molecular purity identification and field purity identification were consistent with each other. The primer marker 532 and 574 could be obtained from the SSR molecular marker method to distinguish the male parent, female parent and hybrid of Xinshikui 6, and both of the 2 primer markers can effectively identify the purities of the hybrid seeds of Xinshikui 6, as well as the authenticity of the seeds. [Conclusion] The proposed method was simple, fast, accurate to operate with the advantages of high reproducibility, and it had become the major method in the identification of sunflower varieties.

Screening and Evaluation of Different Purple Sweet Potato Varieties Specially Used for Processing

Objective] This study almed to screen high-quality and high-yield purpIe sweet potato varieties special y used for processing which were suitabIe for pIanting in Liancheng County. [Method] By using a randomized bIock design, with ‘Longyan 7-3’ as a controI, five purpIe sweet potato varieties were introduced in the autumn of 2012 for comparaton test to evaluate agronomic tralts, economic characters, nu-tritional quality, and processing quantity of dried sweet potato products and fried chips. [Result] ‘Weixiang No.1’ exhibited the highest yield of 41.9 t/hm2, which was improved by 25.8% compared with ‘Longyan 7-3’; fresh tuber yield of ‘Jishu No. 18’ reached 31.6 t/hm2, which was simiIar to that of ‘Longyan 7-3’; dried sweet potato products of ‘Jishu No.18’ and ‘Weixiang No.1’ not onIy had simiIar process stabiIity, taste and coIor to ‘Longyan 7-3’, but also exhibited exceI ent morphoIogy, coIor, transparency, taste and texture, which indicated that these two purpIe sweet potato varieties were suitabIe for processing dried sweet potato products; fried sweet potato chips of ‘Jishu No.18’ and ‘Weixiang No.1’ had moderate oiI content, high expansion degree and simiIar quality to ‘Longyan 7-3’, which indicated that these two purpIe sweet potato varieties were suitabIe for processing fried sweet potato chips; ‘Fushu No.9’, ‘Quanzi No.1’ and ‘Ninzi No.1’ exhibited beautifuI shape, high nutritional quality and good eating quality, which were suitabIe for fresh eating or processing sweet potato powder. [Conclusion] This study provided a scientific ba-sis for Iarge-scale popuIarization and appIication of new purpIe sweet potato vari-eties special y used for processing.

Enhancement of Rice Germplasm by Pyramiding the Cultivars with Multi-resistance to Orseolia oryzae (Wood-Mason)

[ Objective] This study was to breed rice cultivars with multi-resistance to Orseolia oryzae （Wood-Mason）. [ Method] The Guangxi local cultivar GX-M001 （Jiangchao） with high resistance to Orseolia oryzae （Wood-Mason） was used to hybrid with the known resistance cultivars ＂Kangwenqingzhan＂ （harboring GM5 gene）, OB677（ harboring GM3 gene） from Sri Lanka, HT1350 and high yield end quality cultivar ＂ Guiruanzhan＂. [ Result] Through pyramiding the multi-resistant genes via routine hybridization, the general resistances of the hybrids were remarkably enhanced. The grades of resistance were also improved, many of the combinations were endowed with a resistance at immune level （grade 0） ; and interestingly, the respective hybridization of GX-M001 （high resistance） with OB677（ medium resistance） and HT1350（suscepti- ble） also generate two lines at immune level, which is probably the effects of additive effects of genes.[ Conclusion] By routine hybridization, multiple genes were successfully pyramided, thus generating novel rice lines with multiple resistances. For the rice breeding scientists at the grass-roots level, the resistance-resistance pyramiding is an effective approach to breed high resistance cultivars.

Transmission Electron Microscopy Observation of Different Callus Structures in Heiya No.14

[ Objective] The aim was to observe the ultrastructure of different callus structures in Heiya No. 14 by transmission electron microscopy. [Methods] Sample preparation and observation were both carried out by conventional transmission electron microscopy. [ Results] It was showed by transmission electron microscopy that the initial callus cells had a large central vacuole, which squeezed its cytoplasm to be a thin layer around the brim of cell, Meanwhile the nuclear was also squeezed to distribute in the corner of cell, but its nucleolus could be still observed; Compared embryogenic callus with initial callus, its cell wall became thick, and many starch grains and chloroplasts including starch grains could be observed in the cytoplasm area of cell membrane; In non-embryoenic callus, no organelles except for the vacuole could be observed; In browning callus, there was almost no organelles in cells. [ Conclusion] There are significant differences in different types of flax callus at the cell ultrastructure level, which can be as an index for reflecting the differentiation ability of callus cell.

Identification of the Parentage of Corn Variety Using SSR Markers

[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.

Molecular Identification and Cultivar Fingerprints of Prunus persica (L.) Batsch Germplasms

[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.

A Rapid and Sensitive One Step-SYBR Green Based Semi Quantitative Real Time RT-PCR for the Detection of peste des petits ruminants Virus in the Clinical Samples

A sensitive and rapid single step real time （rt） RT-PCR was standardized using one-step Brilliant SYBR Green kit for detection and semi-quantitation ofpeste des petitis ruminants virus （PPRV） using the virus RNA and matrix （M） protein gene-specific primers and compared with established conventional RT-PCR and TaqMan RT-PCR. The assay amplifies a 124 bp fragment of the PPRV M gene with Tm of 78.28 to 78.50. The assay was linear within a range of 50 ng to 0.5 fg total virus RNA with a detection limit （sensitivity） of 0.5 fg. Based on the serial dilution of the live-attenuated PPR vaccine virus, the detection limit was -0.0001 cell culture infectious dose 50% units （TCID50）. Additionally, swab materials spiked with known titre of vaccine virus were equally well detected in the assay. The standardized rt RT-PCR was easily employed for the detection of PPRV nucleic acid directly in the field and experimental clinical samples. The assay detected the PPRV nucleic acid as early as 3 day post infection （dpi） and up to 20 dpi in swab materials from the experimental samples. The assay was rapid and more sensitive than TaqMan and conventional RT-PCR in the detection of PPRV nucleic acid from the PPR suspected clinical samples of sheep and goats. Therefore, the established, simplified SYBR green rt RT-PCR is an alternative test to the already existing various diagnostic assays and could be useful for rapid clinical diagnosis with advantage in reducing risk of contamination.

Response to Low Temperature and Evaluation on Cold Tolerance of Sugarcane at Seedling Stage

This study aimed to screen out cold-tolerant sugarcane cultivars through acomprehensive cold tolerance evaluation. A total of 9 sugarcane cultivars with good agronomic traits, bred by the Yunnan Agricultural University, were selected. They were treated by low temperaturestress （3 ℃）, and the changes of their cold-tolerant physiological and biochemical indices were monitored. The cold tolerance of the sugarcane cultivars was evaluated comprehensively by polar ordination. The results showed that the low temperature stress increased the averagemembrane conductivi- ty, soluble sugar content, MDA content, proline content, soluble protein content, chlorophyll content and peroxidase （POD） activity in sugarcane leaves by 21.21%, 134.1%, 83.60%, 35.47%, 47.72%, 9.07% and 565.2%, respectively, but decreased the superoxide dismutase （SOD） activity in sugarcane leaves by 19.67%. Among the 9 sugarcane cultivars, Dianzhe 03-91 showed the strongest cold tolerance, while Dianzhe 02-39 showed the poorest cold tolerance; the cold tolerance of Dianzhe 08-5, Dianzhe 05-103 and Dianzhe 01-58 was stronger than that of Dianzhe 04- 14, Dianzhe 04-429, Dianzhe 05-522 and Dianzhe 02-227.

Flow-Based Iodometric Extraction Method by the Hollow Fiber Membrane for the Determination of Selenium Sulfide Levels in Cosmeceutical Products

A flow-based iodometric extraction method for the determination of selenium sulfide was developed and applied to cosmeceutical products. Iodine which was generated from the reduction of selenium（IV） ions by iodide ion was on-line extracted using a polypropylene HFM （hollow fiber membrane） liquid extraction technique. The HFM extraction unit was constructed and used to support an organic solvent （hexane） and separate between the organic phase and aqueous phase. The resulting purple extract was carried to a fiber optic spectrophotometric detector for the measurement at 521 nm. Parameters which affected the extraction efficiency, sensitivity and sample throughput such as iodide （selenium molar ratio, extraction time and washing time between the cycles） were investigated and optimized. A linear dynamic range of 80-373 mg.Lt selenium solution was obtained with an extraction time of 60 sec. The total analysis time including washing was about 180 sec which provided a sample throughput of approximately 20 samples＇hr1 and excluded the sample pre-treatment. The recoveries for the determination of selenium in the forms of selenium dioxide and selenium sulfide were in the range of 103%-104% with 1%-3% RSD （relative standard deviation）. The relative errors of this method which was applied for determination of selenium sulfide levels in an anti-dandruff shampoo and a cosmeceutical bead sample were both less than 2.5%.

Strength and deformation behaviors of bedded rock mass under bolt reinforcement

The mechanism of bolt support is an important topic in mining engineering and slope treatment. The artificial material and loading system were self-developed to study the influence of bedding cohesion and bolt number on the anchoring behavior of bedded rock mass. The results show that, both peak strength and elasticity modulus increase gradually with the increase of bedding cohesion and bolt number. The axial stress–strain curve of bedded rock mass under the reinforcement of bolts presents the features of strain-softening and secondary strengthening. Finally, anchoring behavior of bedded rock mass with different bolt numbers was simulated by using FLAC3 D numerical program and the results were compared with the experimental results. This study can provide certain bases to the stability control and support design of bedded rock mass in roadway.