通过低场核磁共振技术与化学计量学相结合的方法对注水猪肉中的水分进行定性、定量检测。通过肌肉注射的方式向猪肉背最长肌中分别注入0%、5%、10%、15%、20%、25%的去离子水。结果表明,注水后不易流动水峰面积比(P21)显著减小,自由水...通过低场核磁共振技术与化学计量学相结合的方法对注水猪肉中的水分进行定性、定量检测。通过肌肉注射的方式向猪肉背最长肌中分别注入0%、5%、10%、15%、20%、25%的去离子水。结果表明,注水后不易流动水峰面积比(P21)显著减小,自由水弛豫时间(T22)、峰面积比(P22)显著增加(P<0.05)。通过一元线性回归(linear regression,LR)模型对猪肉中的水分含量做定量检测,得到相关系数rp为0.31,均方根误差(root mean square error,RMSE)为4.02%,优化后rp为0.45,RMSEp为1.79%。在此基础之上,选取多元线性回归(multiple linear regression,MLR)、偏最小二乘回归预测模型对猪肉中的水分含量进行定量检测,MLR模型得到最优预测结果rp为0.90,RMSEp为0.57%。综上所述,低场核磁共振技术结合化学计量学方法可作为注水猪肉定性、定量检测的一种有效方法。展开更多
Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative PCR.Methods:Total RNA isolated from human HCC and liver tissue adjacent to...Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative PCR.Methods:Total RNA isolated from human HCC and liver tissue adjacent to the tumor was reversely transcribed into cDNA.Real-time fluorescence quantitative PCR(Q-PCR) method was used to analyze the expres-sion level of PRL-2 gene.Results:The Q-PCR method was performed successfully to precisely detect RNA level.PRL-2 was expressed in all portal vein tumor thrombosis(PVTT) and HCC,but only in some paratumor tissue.The highest expression level of PRL-2 gene was recorded in PVTT;meanwhile expression level of PRL-2 was higher than that in paratumor liver tis-sues and in HCC(P < 0.01),and it was higher in HCC than that in paratumor liver tissues.Conclusion:The Q-PCR may be the most precise method to quantitatively detect RNA level and can be used in gene expression changes.The PRL-2 gene has higher expression in PVTT than that in HCC and in paratumor liver tissue cells,indicating that it plays an important role in the development and metastasis of the HCC.展开更多
文摘通过低场核磁共振技术与化学计量学相结合的方法对注水猪肉中的水分进行定性、定量检测。通过肌肉注射的方式向猪肉背最长肌中分别注入0%、5%、10%、15%、20%、25%的去离子水。结果表明,注水后不易流动水峰面积比(P21)显著减小,自由水弛豫时间(T22)、峰面积比(P22)显著增加(P<0.05)。通过一元线性回归(linear regression,LR)模型对猪肉中的水分含量做定量检测,得到相关系数rp为0.31,均方根误差(root mean square error,RMSE)为4.02%,优化后rp为0.45,RMSEp为1.79%。在此基础之上,选取多元线性回归(multiple linear regression,MLR)、偏最小二乘回归预测模型对猪肉中的水分含量进行定量检测,MLR模型得到最优预测结果rp为0.90,RMSEp为0.57%。综上所述,低场核磁共振技术结合化学计量学方法可作为注水猪肉定性、定量检测的一种有效方法。
文摘Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative PCR.Methods:Total RNA isolated from human HCC and liver tissue adjacent to the tumor was reversely transcribed into cDNA.Real-time fluorescence quantitative PCR(Q-PCR) method was used to analyze the expres-sion level of PRL-2 gene.Results:The Q-PCR method was performed successfully to precisely detect RNA level.PRL-2 was expressed in all portal vein tumor thrombosis(PVTT) and HCC,but only in some paratumor tissue.The highest expression level of PRL-2 gene was recorded in PVTT;meanwhile expression level of PRL-2 was higher than that in paratumor liver tis-sues and in HCC(P < 0.01),and it was higher in HCC than that in paratumor liver tissues.Conclusion:The Q-PCR may be the most precise method to quantitatively detect RNA level and can be used in gene expression changes.The PRL-2 gene has higher expression in PVTT than that in HCC and in paratumor liver tissue cells,indicating that it plays an important role in the development and metastasis of the HCC.
