A simple and rapid new method is presented to determine the CO 2/O 2 specificity factor for ribulose_1,5_bisphosphate(RuBP) carboxylase/oxygenase (Rubisco). In principle, the quantitative formula, which intrinsicall...A simple and rapid new method is presented to determine the CO 2/O 2 specificity factor for ribulose_1,5_bisphosphate(RuBP) carboxylase/oxygenase (Rubisco). In principle, the quantitative formula, which intrinsically resulted in error or enormous error in all reported methods, was improved to circumvent the problem. In practice, the complicated procedure with radiolabelling and chromatographic separation of 3_phosphoglycerate and 2_phosphoglycolate was substituted by one_step procedure without radiolabelling and chromatographic separation to decrease the random error. The effects of pH, temperature and ionic strength on accuracy of the data and the reliability of the calculation of the CO 2/O 2 specificity factor are also discussed.展开更多
A method of reliability analysis based on a direct strength calculation employing the von Mises stress failure criterion is presented here. The short term strain distributions of ship hull structural components are id...A method of reliability analysis based on a direct strength calculation employing the von Mises stress failure criterion is presented here. The short term strain distributions of ship hull structural components are identified through the statistical analysis of the wave-induced strain history and the long term distributions by the weighted summation of the short term strain distributions. The wave-induced long term strain distribution is combined with the still water strain. The extreme strain distribution of the response strain is obtained by statistical analysis of the combined strains. The limit state function of the reliability analysis is based on the von Mises stress failure criterion, including the related uncertainties due to the quality of the material and model uncertainty. The reliability index is calculated using FORM and sensitivity analysis of each variable that has effects on the reliability is also discussed.展开更多
文摘A simple and rapid new method is presented to determine the CO 2/O 2 specificity factor for ribulose_1,5_bisphosphate(RuBP) carboxylase/oxygenase (Rubisco). In principle, the quantitative formula, which intrinsically resulted in error or enormous error in all reported methods, was improved to circumvent the problem. In practice, the complicated procedure with radiolabelling and chromatographic separation of 3_phosphoglycerate and 2_phosphoglycolate was substituted by one_step procedure without radiolabelling and chromatographic separation to decrease the random error. The effects of pH, temperature and ionic strength on accuracy of the data and the reliability of the calculation of the CO 2/O 2 specificity factor are also discussed.
文摘A method of reliability analysis based on a direct strength calculation employing the von Mises stress failure criterion is presented here. The short term strain distributions of ship hull structural components are identified through the statistical analysis of the wave-induced strain history and the long term distributions by the weighted summation of the short term strain distributions. The wave-induced long term strain distribution is combined with the still water strain. The extreme strain distribution of the response strain is obtained by statistical analysis of the combined strains. The limit state function of the reliability analysis is based on the von Mises stress failure criterion, including the related uncertainties due to the quality of the material and model uncertainty. The reliability index is calculated using FORM and sensitivity analysis of each variable that has effects on the reliability is also discussed.