The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematocoecus crude extract were investigated, and a spectrophotometric method for precise quantification ...The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematocoecus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba＇s method, alkali treatment destroys chlorophyll. However, we found that： 1） carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2） dimethyl sulfoxide （DMSO）-extracted chlorophyll of green Haematococeus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.展开更多
The mathematic theory for uncertainty model of line segment are summed up to achieve a general conception, and the line error hand model of εσ is a basic uncertainty model that can depict the line accuracy and quali...The mathematic theory for uncertainty model of line segment are summed up to achieve a general conception, and the line error hand model of εσ is a basic uncertainty model that can depict the line accuracy and quality efficiently while the model of εm and error entropy can be regarded as the supplement of it. The error band model will reflect and describe the influence of line uncertainty on polygon uncertainty. Therefore, the statistical characteristic of the line error is studied deeply by analyzing the probability that the line error falls into a certain range. Moreover, the theory accordance is achieved in the selecting the error buffer for line feature and the error indicator. The relationship of the accuracy of area for a polygon with the error loop for a polygon boundary is deduced and computed.展开更多
In this paper, we present a stable single-photon detection method based on Si-avalanche photodiode(Si-APD) operating in Geiger mode with a large temperature variation range. By accurate temperature sensing and direct ...In this paper, we present a stable single-photon detection method based on Si-avalanche photodiode(Si-APD) operating in Geiger mode with a large temperature variation range. By accurate temperature sensing and direct current(DC) bias voltage compensation, the single-photon detector can work stably in Geiger mode from-40 °C to 35 °C with an almost constant avalanche gain. It provides a solution for single-photon detection at outdoor operation in all-weather conditions.展开更多
基金Supported by the Yunnan Provincial Sciences and Technology Department,China (No. 2007AD009)the National Natural Science Foundation of China (No. CNSF30570183)the Knowledge Innovation Program of Chinese Academy of Sciences (No. KSCX2-YW-G-060)
文摘The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematocoecus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba＇s method, alkali treatment destroys chlorophyll. However, we found that： 1） carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2） dimethyl sulfoxide （DMSO）-extracted chlorophyll of green Haematococeus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.
基金Project supported by the National Natural Science Foundation of China (No.40301043) .
文摘The mathematic theory for uncertainty model of line segment are summed up to achieve a general conception, and the line error hand model of εσ is a basic uncertainty model that can depict the line accuracy and quality efficiently while the model of εm and error entropy can be regarded as the supplement of it. The error band model will reflect and describe the influence of line uncertainty on polygon uncertainty. Therefore, the statistical characteristic of the line error is studied deeply by analyzing the probability that the line error falls into a certain range. Moreover, the theory accordance is achieved in the selecting the error buffer for line feature and the error indicator. The relationship of the accuracy of area for a polygon with the error loop for a polygon boundary is deduced and computed.
基金supported by the National Natural Science Foundation of China(No.11374105)
文摘In this paper, we present a stable single-photon detection method based on Si-avalanche photodiode(Si-APD) operating in Geiger mode with a large temperature variation range. By accurate temperature sensing and direct current(DC) bias voltage compensation, the single-photon detector can work stably in Geiger mode from-40 °C to 35 °C with an almost constant avalanche gain. It provides a solution for single-photon detection at outdoor operation in all-weather conditions.
