AIM:To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells.METHODS:Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate...AIM:To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells.METHODS:Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate at the ratio of 2:1) for 24 h.Cytotoxicity and apoptosis were evaluated by 3-(4,5-dmethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and Annexin V/propidium iodide staining,respectively.Cellular total lipid was determined using a photocolorimetric method after Nile red staining,and triglyceride content was measured using an enzymatic kit.To study the effects of Sch B on steatosis,L-02 cells were treated with Sch B (1-100 μmol/L) in the absence or presence of 1 mmol/L FFA for 24 h,and cellular total lipid and triglyceride levels were measured.To explore the mechanisms of action of Sch B in the steatotic L-02 cells,mRNA levels of several regulators of hepatic lipid metabolism including adipose differentiation related protein (ADRP),sterol regulatory element binding protein 1 (SREBP-1),peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ were measured by quantitative real-time polymerase chain reaction (PCR),and protein levels of ADRP and SREBP-1 were measured by immunoblotting.RESULTS:Treatment with 1 mmol/L FFA for 24 h induced intracellular lipid accumulation in L-02 cells comparable to that in human steatotic livers without causing apparent apoptosis and cytotoxicity.Sch B mitigated cellular total lipid and triglyceride accumulations in the steatotic L-02 cells in a dose-dependent manner.Quantitative real-time PCR and Western blot analyses revealed that treatment of L-02 cells with 100 μmol/L Sch B reverted the FFA-stimulated up-regulation of ADRP and SREBP-1.CONCLUSION:Sch B inhibits FFA-induced steatosis in L-02 cells by,at least in part,reversing the up-regulation of ADRP and SREBP-1.展开更多
In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88genes. After ...In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes werefirstly associated with UL. Three genes with notable difference were selected for Northern confirmationOur results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showedup-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obviousexpression in prostate, testis, liver, heart and skeletal muscle.展开更多
Rainfall infiltration depth and mode can severely influence slope stability.With the sustained rainfall,the influenced region of slope gradually expands.By using the Green-Ampt model to the soil slope,infiltration reg...Rainfall infiltration depth and mode can severely influence slope stability.With the sustained rainfall,the influenced region of slope gradually expands.By using the Green-Ampt model to the soil slope,infiltration regulation was discussed under sustained and small intensity rainfall.And the infiltration rate of unsaturated soil was proposed according to the saturated infiltration theory.Because of the changing of initial moisture content in depth of slope,the saturated or unsaturated infiltration rate and depth could also be changeable with the sustained rainfall infiltration.Based on the principle of strength reduction,the calculation model of slope safety factor was established under different initial moisture contents and infiltration modes.Then,the slope stability was quantitatively analyzed through software FLAC3D.The calculation results of soil slope engineering show that there is a shorter period for slope stability under different initial moisture contents and unsaturated infiltration ways at the slope wetting front.The stability period of slope is 33.3%according to different initial moisture contents of wetting front less than that of the same initial moisture content of wetting front.And the slope is easier to fail under the unsaturated infiltration.The results agree well with the actual situation under sustained and small intensity rainfall.展开更多
基金Supported by The Hong Kong Baptist University,No.FRG 08-09/II-30
文摘AIM:To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells.METHODS:Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate at the ratio of 2:1) for 24 h.Cytotoxicity and apoptosis were evaluated by 3-(4,5-dmethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and Annexin V/propidium iodide staining,respectively.Cellular total lipid was determined using a photocolorimetric method after Nile red staining,and triglyceride content was measured using an enzymatic kit.To study the effects of Sch B on steatosis,L-02 cells were treated with Sch B (1-100 μmol/L) in the absence or presence of 1 mmol/L FFA for 24 h,and cellular total lipid and triglyceride levels were measured.To explore the mechanisms of action of Sch B in the steatotic L-02 cells,mRNA levels of several regulators of hepatic lipid metabolism including adipose differentiation related protein (ADRP),sterol regulatory element binding protein 1 (SREBP-1),peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ were measured by quantitative real-time polymerase chain reaction (PCR),and protein levels of ADRP and SREBP-1 were measured by immunoblotting.RESULTS:Treatment with 1 mmol/L FFA for 24 h induced intracellular lipid accumulation in L-02 cells comparable to that in human steatotic livers without causing apparent apoptosis and cytotoxicity.Sch B mitigated cellular total lipid and triglyceride accumulations in the steatotic L-02 cells in a dose-dependent manner.Quantitative real-time PCR and Western blot analyses revealed that treatment of L-02 cells with 100 μmol/L Sch B reverted the FFA-stimulated up-regulation of ADRP and SREBP-1.CONCLUSION:Sch B inhibits FFA-induced steatosis in L-02 cells by,at least in part,reversing the up-regulation of ADRP and SREBP-1.
文摘In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes werefirstly associated with UL. Three genes with notable difference were selected for Northern confirmationOur results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showedup-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obviousexpression in prostate, testis, liver, heart and skeletal muscle.
基金Project(2010(A)06-b)supported by the Scientific Research Fund of Yunnan Provincial Transport Department of ChinaProject(51108293)supported by the National Natural Science Foundation of China+1 种基金Project(2013PY37)supported by the Cultivated Foundation of Taizhou University of ChinaProject(LY13E080008)supported by the Zhejiang Provincial Natural Science Foundation of China
文摘Rainfall infiltration depth and mode can severely influence slope stability.With the sustained rainfall,the influenced region of slope gradually expands.By using the Green-Ampt model to the soil slope,infiltration regulation was discussed under sustained and small intensity rainfall.And the infiltration rate of unsaturated soil was proposed according to the saturated infiltration theory.Because of the changing of initial moisture content in depth of slope,the saturated or unsaturated infiltration rate and depth could also be changeable with the sustained rainfall infiltration.Based on the principle of strength reduction,the calculation model of slope safety factor was established under different initial moisture contents and infiltration modes.Then,the slope stability was quantitatively analyzed through software FLAC3D.The calculation results of soil slope engineering show that there is a shorter period for slope stability under different initial moisture contents and unsaturated infiltration ways at the slope wetting front.The stability period of slope is 33.3%according to different initial moisture contents of wetting front less than that of the same initial moisture content of wetting front.And the slope is easier to fail under the unsaturated infiltration.The results agree well with the actual situation under sustained and small intensity rainfall.
