A reverse transcription polymerase chain reaction (RT PCR) was used for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) from the gills and subcutaneous tissue of Penaeus vannamei (10-15cm ...A reverse transcription polymerase chain reaction (RT PCR) was used for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) from the gills and subcutaneous tissue of Penaeus vannamei (10-15cm in body size) without clinical signs. With primers 77012R and 77353F, a fragment of 356bp of IHHNV was amplified in RT PCR. The amplified product was sequenced and showed more than 98% homologue with the sequences of other IHHNV strains in Gene Bank.展开更多
目的应用实时荧光定量逆转录聚合酶链反应(RT-PCR)检测兔火器伤骨折愈合过程中骨形态发生蛋白(BMP)-2mRNA水平。方法建立外固定架固定一期治疗兔股骨火器伤骨折的动物模型和BMP-2 mRNA表达水平的SYBR G reenⅠ荧光定量RT-PCR方法,分别...目的应用实时荧光定量逆转录聚合酶链反应(RT-PCR)检测兔火器伤骨折愈合过程中骨形态发生蛋白(BMP)-2mRNA水平。方法建立外固定架固定一期治疗兔股骨火器伤骨折的动物模型和BMP-2 mRNA表达水平的SYBR G reenⅠ荧光定量RT-PCR方法,分别于术后第1、2、3、4周取断端组织,通过实时荧光定量RT-PCR检测标本标本中BMP-2 mRNA水平。结果在对照组,创伤后的兔骨组织BMP-2 mRNA表达水平在1周开始增高,在第2周达到高峰后下降,第4周即可恢复正常。而实验组,受枪伤的兔骨组织BMP-2 mRNA表达水平在第3周表达最高,与对照组相比,升高较慢,表达量也偏低(P<0.05)。结论BMP-2mRNA SYBR G reenⅠ荧光定量RT-PCR可以很好地反应枪击伤对兔骨组织BMP-2 mRNA表达水平的影响。枪击伤后的兔骨组织BMP-2 mRNA表达水平与对照组相比升高较慢,表达量也偏低。展开更多
文摘A reverse transcription polymerase chain reaction (RT PCR) was used for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) from the gills and subcutaneous tissue of Penaeus vannamei (10-15cm in body size) without clinical signs. With primers 77012R and 77353F, a fragment of 356bp of IHHNV was amplified in RT PCR. The amplified product was sequenced and showed more than 98% homologue with the sequences of other IHHNV strains in Gene Bank.
