面向均质实体的产品轮廓反求工程

提出了一个新型的产品无损测量轮廓反求逆向工程系统,应用轮廓数字化单元体素体分层测量方法,以液体作为测量介质,运用阿基米德定律、杠杆原理和重力矩理论,并利用光学系统采集图像,通过计算机图像处理技术获取边缘轮廓,结合重力矩方程、重心方程进行推算,求出空间各单元体素体的空间坐标,利用相应的CAD软件处理系统进行三维模型的重建。研究了面向均质实体的产品轮廓反求系统的原理、组成和系统软件与硬件的设计。

基于均质实体的切片轮廓非接触三维测量方法

本文设计了一种基于均质实体的片层轮廓测量方法,阐述了该方法的测量原理,由阿基米德定律和杠杆原理,利用电子天平逐层测算被测实体浸入液面不同深度时所受浮力,通过计算机计算相应每片层的重力矩、重心等信息,推算出各层面上微小实体单元在三维坐标系中的位置,进而重构出被测实体的片层轮廓。经过实验验证,其结果证明了该方法是切实可行的。

基于静力平衡原理的三维均质实体测量方法

提出一种基于静力平衡原理的三维均质实体无损测量方法,测量方法根据杠杆平衡系统中力的变化间接实现无损分层测量,以杠杆平衡系统中力与力矩平衡和其与实体重量的关系为基础,通过按一定方向越过支点微小位移,测量杠杆平衡系统中实体每个不同位置的受力变化大小,求解被测实体各片层的质量和相应片层的重心坐标值,建立各片层质量和所含微小单元体的方程组及重心坐标方程组。然后通过智能计算求解方程组,获得各单元体的质量和空间坐标值,进而对获得的点云数据进行图像重构。重点论述了基于静力平衡原理的三维均质实体无损测量方法的测量原理、组成和其数学模型的建立及其求解分析。

IGES文件在非均质实体保存与读取中的应用

非均质实体是一种具有多种优良性能的新型构件,但由于其数据处理和记录方式与常规均质实体具有本质区别,导致了非均质实体建模软件与其它常规CAD/CAM软件之间的数据传输与数据交换十分困难,严重影响着非均质实体建模软件的通用性.针对这一问题,基于IGES文件,提出了一种能够保存非均质实体模型的几何信息和材质信息的方法,解决了非均质实体的保存与读取以及数据共享问题,实现了不同平台间的非均质材料实体的数据交换.

基于材料特征表达的非均质实体模型建模

为表达生物材料的非均质性,提出基于材料特征的非均质实体模型。材料特征作为设计意图的高层抽象,用以表达实体某一特定子区域的材料分布。材料特征通过材料组分分布函数来表达,讨论了材料组分分布函数的定义、形式和定义域。提出材料特征的三层模型,并对材料特征进行了分类,讨论了特征对应的函数表达式和定义域等要素。借助开放源码的图形核心VTK(V isua lization T oo lK it)实现了一个原型建模系统,系统继承了特征建模的优点,使设计者可以根据设计规则、功能需求直观地设计实体内部的材料梯度。

Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes

AIM： To investigate the possible mechanism for HBV X gene to induce apoptosis of hepatocyte HL-7702 cells.METHODS： HBV X gene eukaryon expression vector pcDNA3-X was established and transfected into HL-7702 cells by lipid-mediated transfection, including transient and stable transfection. Positive clones were screened by incubating in the selective medium with 600 μg/mL G418 and named HL-7702/HBV-encoded X protein （HBx） cells. The expressions of Fas/FasL, Bax/Bcl-2, and c-myc mRNA were measured by semi-quantitative RT-PCR in HL-7702/HBx and control group, respectively.RESULTS： RT-PCR analysis confirmed that HBV X gene was transfected into HL-7702 cells successfully. By semiquantitative RT-PCR analysis, Bax and c-myc mRNA levels in HL-7702/HBx cells of transient transfection were significantly higher than those in control, FasL and c-myc mRNA levels in HL-7702/HBx cells of stable transfection were significantly higher than those in control, whereas the Bcl-2 mRNA levels in HL-7702/HBx cells of transient and stable transfection were significantly lower than thosein control.CONCLUSION： HBV X gene may promote the apoptosis of hepatocytes by regulating the expressions of Fas/FasL, Bax/Bcl-2, and c-myc gene in a dose-dependent manner.

Mass Transfer Enhancement of Gas Absorption by Adding the Dispersed Organic Phases

Mass transfer enhancement of gas absorption by adding a dispersed organic phase has been studied in this work. Various dispersed organic phases （heptanol, octanol, isoamyl alcohol, heptane, octane, and isooctane） were tested respectively in the experiment. According to the theoretical model and experimental data, the overall volumetric mass transfer coefficient and enhancement factor were obtained under different dispersed organic phase volume fraction and stirring speed. The experimental results indicate that gas-liquid mass transfer is enhanced at different level by adding a dispersed organic phase. The best performance of enhancement were achieved with the dispersed organic phase volumetric fraction of 5% and under an intermediate stirring speed of 670 r·min^-1. Among the organic phases tested in the experiment, alcohols show better performance, which gave 20% higher enhance-ment of overall volumetric mass transfer coefficient than adding alkanes.

Conscious Entities in Superluminal Parallel Universe

Tachyons are theoretical superluminal particles, i.e., faster than light particles. The idea of existence of tachyons comes from Arnold Sommerfeld. Bilaniuk and Sudarshan （1969） published the article ＂Particles beyond the Light Barrier＂ in Physics Today magazine where they analyze the theoretical properties of tachyons. From the tachyon idea I have hypothesized the existence of a Superluminal Parallel Universe constituted by Superluminal Conscious Entities that, like the theoretical tachyons, only can exist at speeds greater than the speed of light. This hypothesis should be subject to scientific inquiry but also to philosophical speculation or spiritual imagination. Later I imagine that the Superluminal Parallel Universe is the Spiritual World thought by theologians and idealistic philosophers. I relate the Superluminal Conscious Entities with Spirits and the Superluminal Parallel Universe comprising infinity of Superluminal Conscious Entities with God, the Universal Spirit, thus being simultaneously Unity and Infinity given that superluminal speed implies non-local, instantaneous connections. I imagine that the material Universe might have emerged from that God, assuming that many Spirits could lose superluminality generating the two classes of substances of the Universe： the class of subluminal particles of matter and the class of luminal particles, like photons. I imagine a Soul as a quasi superluminal entity dropped from a Spirit to animate material entities. The substances of both Universes would have evolved in parallel, with life arising and evolving from the subluminal substance driven by companion Souls. Let＇s also imagine that the natural way of being of Spirits and free Souls is a state similar to conscious dreaming. In this imagination, we all, Souls, would have been a part of God and we all might be again. We all are makers of the Universe. We all might get connected with the Spirits in God to beg for and to receive inspiration.

Vapor Pressure Measurement and Correlation of 2-Methyl-Butanol Acetate Containing Calcium Chloride

The CaCl2 solubility in 2-methyl-butanol acetate and the vapor pressure of 2-methyl-butanol acetate containing CaCl2 were measured in the range of 90-135°C and from very low salt concentration to saturation.The experimental data were correlated with two equations,a modified Antoine equation with the dissolved salt taken into account and a nonrandom two liquid-electrolyte（e-NRTL）model.Both models are in good agreement with the experimental data.This study provides essential physical data for further investigation of vapor-liquid equilibrium system containing salt.

Laboratory Research on Tahe AR Fluid Coking on Weak Acid Catalysts

Fluid coking on micro-spherical particles with acid sites on them could produce more light oils from Tahe AR.The conversion rate could increase by about 20% on the catalyst B compared to that obtained on the catalyst A and the light oil yield could increase by about 12%.The yield of gasoline and diesel was more than 50% from Tahe AR over the catalyst B.Tests on acidity of the catalyst B by pyridine FT-IR spectrometry showed that the total acid content and the ratio of weak acid number to total acid number were higher than other catalysts.

The flocculation research on treating suspended solids contained mine drainage through goaf

Mine drainage could be filtered and purified through goaf. This innovative technique shows merits, such as high treatment efficiency, remarkable economic benefit and extensive wastewater recycle use. However, it was detected that capacities of purifying mine drainage for goaf were decreased after a period of application. As a result, the effluent could not meet the standard of recycle water. To solve the problem, coagulant was considered to add into mine drainage reducing its high turbid degrees to certain level. After the preliminary flocculation treatment, mine drainage was piped into goaf to purify. In this way, the load of goaf was eased up. Its usage time was also prolonged. Therefore, this paper carried out the coagulation-flocculation jar test for mine drainage to discuss the flocculation parameters. By the experiment, 10 % iron trichloride is selected from four inorganic coagulants as the optimum coagulant. The optimum dose, PH value and sedirs6-7 and 25 min. Velocity mentation time are respectively 2 mL per 800 mL Wastewater gradient G during the process of mixing and reaction is 696 .And the value of GT is 6.264 × 10^5. The values of G and GT will supply the basis for the design of flocculation pool in the project. The flocculation parameters will be significant for the reference of practice.

Imaging protein crystal growth behaviour in batch cooling crystallisation

The temporal and spatial growth behaviour of protein crystals, subject to different cooling strategies in protein crystallisation was investigated. Although the impact of temperature and cooling rate on crystal growth of small molecules was well documented, much less has been reported on their impact on the crystallisation of proteins. In this paper, an experimental set-up is configured to carry out such a study which involves an automatic temperature controlled hot-stage crystalliser fitted with a real-time imaging system. Linbro parallel crystallisation experiments(24-well plate) were also conducted to find the suitable initial conditions to be used in the hot-stage crystallisation experiments, including the initial concentration of HEW lysozyme solutions, precipitate concentration and pH value. It was observed that fast cooling rates at the early stage led to precipitates while slow cooling rates produced crystal nuclei, and very slow cooling rates, much smaller than for small molecules are critical to the growth of the nuclei and the crystals to a desired shape. The interesting results provide valuable insight as well as experimental proof of the feasibility and effectiveness of cooling as a means for achieving controlled protein crystallisation, compared with the evaporation approach which was widely used to grow single large crystals for X-ray diffraction study. Since cooling rate control can be easily achieved and has good repeatability, it suggests that large-scale production of protein crystals can be effectively achieved by manipulating cooling rates.

Effect of naked eukaryotic expression plasmid encoding rat augmenter of liver regeneration on acute hepatic injury and hepatic failure in rats

AIM: To study the protective effect of eukaryotic expression plasmid encoding augmenter of liver regeneration (ALR) on acute hepatic injury and hepatic failure in rats. METHODS: The PCR-amplified ALR gene was recombined with pcDNA3 plasmid, and used to treat rats with acute hepatic injury. The rats with acute hepatic injury induced by intraperitoneal injection of 2 mL/kg 50% carbon tetrachloride (CCl4) were randomly divided into saline control group and recombinant pcDNA3-ALR plasmid treatment groups. Recombinant pcDNA3-ALR plasmid DNA (50 or 200 μg/kg) was injected into the rats with acute hepatic injury intravenously, intraperitoneally, or intravenously and intraperitoneally in combination 4 h after CCl4 administration, respectively. The recombinant plasmid was injected once per 12 h into all treatment groups four times, and the rats were decapitated 12 h after the last injection. Hepatic histopathological alterations were observed after HE staining, the expression of proliferating cell nuclear antigen (PCNA) in liver tissue was detected by immunohistochemical staining, and the level of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was determined by biochemical method. The recombinant plasmid DNA (200 μg/kg) and saline were intraperitoneally injected into the rats with acute hepatic failure induced by intraperitoneal injection of 4 mL/kg 50% CCl4 after 4 h of CCl4 administration, respectively. Rats living over 96 h were considered as survivals.RESULTS: The sequence of ALR cDNA of recombinant pcDNA3-ALR plasmid was accordant with the reported sequence of rat ALR cDNA. After the rats with acute hepatic injury were treated with recombinant pcDNA3-ALR plasmid, the degree of liver histopathological injury markedly decreased. The pathologic liver tissues, in which hepatic degeneration and necrosis of a small amount of hepatocytes and a large amount of infiltrating inflammatory cells were observed, and they became basically normal in the most effective group after four times of injection of recombinant pcDNA3-ALR plasmid. The indexes of PCNA significantly increased in the recombinant pcDNA3-ALR plasmid treatment groups compared to model group. The level of serum AST and ALT remarkably reduced in recombinant pcDNA3-ALR plasmid treatment groups compared to model group. The results showed that the effect of 200 μg/kg recombinant pcDNA3-ALR plasmid in the rats with acute liver injury was stronger than that of 50μg/kg pcDNA3-ALR DNA.The effect of intravenous injection of recombinant pcDNA3ALR plasmid was better. After the rats with acute hepatic failure were treated with recombinant pcDNA3-ALR plasmid,the survival rate (40%) significantly increased in treatment groups compared to control group (15%, P＜0.01).CONCLUSION: The ALR gene may play an important role in relieving acute hepatic injury and hepatic failure by promoting hepatic cell proliferation and reducing level ofAST and ALT in CCl4-intoxicated rats.

Application of Functional Training in College Volleyball Teaching

Volleyball Teaching in Colleges and universities is faced with multiple challenges, such as the teaching content is simple, the training method is single, the students’ physical quality is poor, the physical condition of the physical condition is the guarantee of volleyball technology. This research is on body function training on volleyball teaching methods of this technology in the project, the function of training system into the volleyball technique teaching, through the method of experimental teaching method to verify the function of training to the technical level of the student volleyball has been effectively improved.

Gas–Liquid Mass Transfer Characteristics in a Gas–Liquid–Solid Bubble Column under Elevated Pressure and Temperature

abstract The volumetric mass transfer coefficient kLa of gases （H2, CO, CO2） and mass transfer coefficient kL on liquid par-affin side were studied using the dynamic absorption method in slurry bubble column reactors under elevated temperature and elevated pressure. Meanwhile, gas-holdup and gas-liquid interfacial area a were obtained. The effects of temperature, pressure, superficial gas velocity and solid concentration on the mass transfer coeffi-cient were discussed. Experimental results show that the gas-liquid volumetric mass transfer coefficient kLa and interfacial area a increased with the increase of pressure, temperature, and superficial gas velocity, and decreased with the slurry concentration. The mass transfer coefficient kL increased with increasing superficial gas velocity and temperature and decreased with higher slurry concentration, while it changed slightly with pressure. Ac-cording to analysis of experimental data, an empirical correlation is obtained to calculate the values of kLa for H2 （CO, CO2） in the gas-paraffin-quartz system in a bubble column under elevated temperature and elevated pressure.

The Influence of Glucose on Numerical Simulation of a Vascular Solid Tumor Growth

Glucose is the mainly nutrient substances in tumor growth,which played an important role in tumor cells' growth,proliferation and immigration.Numerical simulation will help a good understanding for the influence of glucose which affected on a vascular solid tumor growth.We present a hybrid on-Lattice Model to simulate the influence of glucose on a-vascular tumor growth.The hybrid model we developed focuses on five key variables implicated in the invasion process:tumor cells,extracellular matrix,matrix-degradative enzymes,oxygen and glucose.And about the discrete model,we consider cell evolution dynamics on cell level.Results indicate that the number of proliferation and quiescent cells is decreasing by decreasing the initial glucose concentration,consequently increase necrotic area relatively.Thus there is inhabitation effect on tumor growth by decreasing initial glucose concentration.

The Evaluation System Research based on the Working Process Systematization of the Practice Teaching Quality

The design philosophy based on the working process systematization, the feature of practice teaching is analyzed the principles of practice teaching quality evaluation system are summed up. The evaluation system based on working process systematization of the practice teaching quality is established, the management of quality evaluation system and the project of the monitoring are put forward.

An overview of the thermography-based experimental studies on roadway excavation in stratified rock masses at CUMTB

This paper presents an overview of experimental investigations conducted at China University of Mining and Technology Beijing(CUMTB) on roadway excavation using large-scale geomechanical model tests.The simulated sedimentary rocks are composed by alternating layers of sandstone, mudstone and coal seam inclined at varied angles with respect to the horizontal including 0°, 45°, 60°, and 90°. During the excavation, infrared thermography was employed to detect the thermal response of the surrounding rocks under excavation. The obtained raw thermograms were processed using denoising algorithm, data reduction procedure and Fourier analysis. The infrared temperature(IRT) characterizes the overall rock response; the processed thermal images represent the structural behavior, and the Fourier spectrum describes damage development in the frequency domain. Deeper understanding was achieved by the comparative analyses of excavation in differently inclined rock masses using the image features of IRTs, thermal images and Fourier spectra.

THE RELATIONSHIPS BETWEEN ERYTHROBLAST DENUCLEATION AND THE NUCLEAR MATRIX－INTERMEDIATE FILAMENTS

We present a novel approach for making cybrids. By introducing neo gene expression plasmids into rabbit reticulocytes, fusing the gene transferred reticulocytes with K562 cells and selecting in G418 selection medium, a cybrid strain K-RRneo was established. Whole mount TEM study demonstrated that after cybridization, there was a reorganization of the intermediate filaments which showed a tendency to differentiate towards reticulocytes. SDS-PAGE and western blot analysis verified the above observation, in which the vimentin blot pattern of the cybrids was similar to that of reticulocytes, but totally different from that of K562 cells. Using this model, we reaffirmed the hypothesis that the erythroid differentiation factor (EDF) might be responsible for erythroid differentiation as well as the initiation of denucleation.

Determination of Bed Void Fraction from Fixed Bed Breakthrough Curve

Accurate physical and chemical properties of adsorbents are required for the efficient design of fixed bed adsorbers. The bed void and particle void are significant physical properties. One of the experimentally determination technique for the bed void is a breakthrough curve method. In the present paper, experimental conditions for determining the bed void from breakthrough curve were investigated because complex conditions are required. The values of bed void were determined from theoretical elution curve at the point of c/c0 = 0.5 under the condition of negligible amount adsorbed. The bed void value determined from elution curve was clearly affected by particle void and fluid velocity, and regarded as ＂apparent＂ bed void values. For large porosity particles, it was difficult to determine the true bed void value due to the effect of penetration into macro pores. Then, the bed void fraction can be determined safely from breakthrough curve when particles possessing small particle void are used.