[Objective] The aim was to investigate the difference in MSTN gene expression in different tissues of Tibetan sheep at different ages.[Method] According to the sequence（NM_001009428.1）published in GenBank,a pair of ...[Objective] The aim was to investigate the difference in MSTN gene expression in different tissues of Tibetan sheep at different ages.[Method] According to the sequence（NM_001009428.1）published in GenBank,a pair of specific primers was designed to amplify part of cDNA sequence of MSTN by using QRT-PCR technique.The relative expression level of MSTN gene in rennet stomach,rumen,leg muscle and cardiac muscle of Tibetan sheep at different ages were analyzed.[Result] After normalization with β-actin gene,the relative expression level of MSTN gene in the 6-month-old Tibetan sheep was the highest and it was 2.52 times than that in 12-month-old Tibetan sheep（P0.05）,the relative expression level of MSTN gene in leg muscle was the highest among all tissues and it was 3 984.78 times than that in rumen（P0.01）.[Conclusion] The results established theoretical foundation for the correct use of MSTN antibody.展开更多
The effect of time and environment on the dimension precision and mass of LOM prototypes was experimentally investigated.It is to identify the stability of the dimension of LOM prototypes after forming.The results sho...The effect of time and environment on the dimension precision and mass of LOM prototypes was experimentally investigated.It is to identify the stability of the dimension of LOM prototypes after forming.The results show that the dimension and the mass tendency to grow,which is mainly caused by elastic recovery and moisture absorption and is characterized principally by the growth of Z dimension.Self restraint can be a significant factor to influence Z growth of LOM prototypes.展开更多
基金Supported by Key Project of Applied Basic Research of Sichuan Province(07JY029-045)~~
文摘[Objective] The aim was to investigate the difference in MSTN gene expression in different tissues of Tibetan sheep at different ages.[Method] According to the sequence（NM_001009428.1）published in GenBank,a pair of specific primers was designed to amplify part of cDNA sequence of MSTN by using QRT-PCR technique.The relative expression level of MSTN gene in rennet stomach,rumen,leg muscle and cardiac muscle of Tibetan sheep at different ages were analyzed.[Result] After normalization with β-actin gene,the relative expression level of MSTN gene in the 6-month-old Tibetan sheep was the highest and it was 2.52 times than that in 12-month-old Tibetan sheep（P0.05）,the relative expression level of MSTN gene in leg muscle was the highest among all tissues and it was 3 984.78 times than that in rumen（P0.01）.[Conclusion] The results established theoretical foundation for the correct use of MSTN antibody.
文摘The effect of time and environment on the dimension precision and mass of LOM prototypes was experimentally investigated.It is to identify the stability of the dimension of LOM prototypes after forming.The results show that the dimension and the mass tendency to grow,which is mainly caused by elastic recovery and moisture absorption and is characterized principally by the growth of Z dimension.Self restraint can be a significant factor to influence Z growth of LOM prototypes.
