[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with...[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with PCV2 in vivo. [Method] Healthy 40-day-old Landrace piglets were infected with porcine circovirus type 2 （PCV2） and euthanized on the 34, 7rd, 14th, 21st and 35th d post inoculation （DPI）. The porcine skin-derived dendritic cells （DCs） were collected to analyze the transcrip- tional levels of molecules （LMP7, UBP, MHC-I, calreticulin） associated with endogenous antigen processing and presentation by using real-time fluorescent quantitative PCR （real-time FQ-PCR）. [Result] The results showed that the level of LMP7 mR- NAs was reduced significantly on the 3DPI （P〈0.05）; the level of UBP mRNAs was consistently up-regulated, which increased significantly on the 21DPI and 35DPI （P〈 0.05）; the level of MHC-I mRNAs was significantly down-regulated on the 7DPI （P〈 0.05）; the level of calreticulin mRNAs was up-regulated slightly without significant dif- ference. [Conclusion] PCV2 can inhibit the endogenous antigen processing and presentation ability of porcine skin-derived DCs at early stages of infection.展开更多
Y2001-62856-775 0119961人工指导下的实时加工=Real-time rendering of artifi-cial guidance[会,英]/Yaan,X.B.//2000 IEEE Inter-national Conference on Systems.Man and Cybernet-ics.—775～780(NiK)虚拟装配研发了一种计算机工具...Y2001-62856-775 0119961人工指导下的实时加工=Real-time rendering of artifi-cial guidance[会,英]/Yaan,X.B.//2000 IEEE Inter-national Conference on Systems.Man and Cybernet-ics.—775～780(NiK)虚拟装配研发了一种计算机工具,它能够辅助人在虚拟环境中完成机械装配的任务。本文研究了计算饥综合指导下的实时加工,它能利用生物激励神经网络产生避撞路径,帮助用户通过指导物体的运动规定装配任务。本文工作揭示了人工指导下“计算机制造”的能力。展开更多
CNC machining plays an important role in mechanical manufacturing.A key issue is to improve the machining feedrate while keeping the machining precision and satisfying the acceleration constraints of the CNC machine.F...CNC machining plays an important role in mechanical manufacturing.A key issue is to improve the machining feedrate while keeping the machining precision and satisfying the acceleration constraints of the CNC machine.For the consecutive micro-line segments interpolation,the velocities at the junction of two segments are the bottlenecks for the machining efficiency.This paper proposes a multi-period turning method to improve the feedrate at the junctions using the linear acceleration and deceleration mode,which utilizes the maximal acceleration capabilities of the NC machine while satisfying the machining precision.A new and more efficient look-ahead method and a feedrate override method are also proposed to boast the global machining speed.The proposed algorithm has been implemented on Blue Sky NC System,and experimented in real material manufacturing.Compared with several existing algorithms,the current algorithm can improve the manufacturing time ranging from 50% to 180%,depending on the machining parameters,and also results in better machining quality.In addition,the algorithm also satisfies the need of real-time interpolation.展开更多
The effects of different refrigerants on heat transfer performance of pulsating heat pipe(PHP) are investigated experimentally.The working temperature of pulsating heat pipe is kept in the range of 20℃-50℃.The start...The effects of different refrigerants on heat transfer performance of pulsating heat pipe(PHP) are investigated experimentally.The working temperature of pulsating heat pipe is kept in the range of 20℃-50℃.The startup time of the pulsating heat pipe with refrigerants can be shorter than 4 min,when heating power is in the range of 10W-100W.The startup time decreases with heating power.Thermal resistances of PHP with filling ratio 20.55% were obviously larger than those with other filling ratios.Thermal resistance of the PHP with R134a is much smaller than that with R404A and R600a.It indicates that the heat transfer ability of R134a is better.In addition,a correlation to predict thermal resistance of PHP with refrigerants was suggested.展开更多
基金Supported by Natural Science Foundation of Beijing "Effect of porcine skin-derived dendritic cells on PCV infection" (6062006)Beijing Organization Department Project"Influence of PCV infection on bone marrow cell differentiation" (20061D0502100282)~~
文摘[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with PCV2 in vivo. [Method] Healthy 40-day-old Landrace piglets were infected with porcine circovirus type 2 （PCV2） and euthanized on the 34, 7rd, 14th, 21st and 35th d post inoculation （DPI）. The porcine skin-derived dendritic cells （DCs） were collected to analyze the transcrip- tional levels of molecules （LMP7, UBP, MHC-I, calreticulin） associated with endogenous antigen processing and presentation by using real-time fluorescent quantitative PCR （real-time FQ-PCR）. [Result] The results showed that the level of LMP7 mR- NAs was reduced significantly on the 3DPI （P〈0.05）; the level of UBP mRNAs was consistently up-regulated, which increased significantly on the 21DPI and 35DPI （P〈 0.05）; the level of MHC-I mRNAs was significantly down-regulated on the 7DPI （P〈 0.05）; the level of calreticulin mRNAs was up-regulated slightly without significant dif- ference. [Conclusion] PCV2 can inhibit the endogenous antigen processing and presentation ability of porcine skin-derived DCs at early stages of infection.
文摘Y2001-62856-775 0119961人工指导下的实时加工=Real-time rendering of artifi-cial guidance[会,英]/Yaan,X.B.//2000 IEEE Inter-national Conference on Systems.Man and Cybernet-ics.—775～780(NiK)虚拟装配研发了一种计算机工具,它能够辅助人在虚拟环境中完成机械装配的任务。本文研究了计算饥综合指导下的实时加工,它能利用生物激励神经网络产生避撞路径,帮助用户通过指导物体的运动规定装配任务。本文工作揭示了人工指导下“计算机制造”的能力。
基金supported by the National Key Basic Research Project of China (Grant Nos 2011CB302400)the National Natural Science Foundation of China (Grant Nos 60821002, 10871195, 10925105)+1 种基金Major National S&T Project "Advanced CNC Systems"CAS Project "MM Methods for Advanced CNC Systems"
文摘CNC machining plays an important role in mechanical manufacturing.A key issue is to improve the machining feedrate while keeping the machining precision and satisfying the acceleration constraints of the CNC machine.For the consecutive micro-line segments interpolation,the velocities at the junction of two segments are the bottlenecks for the machining efficiency.This paper proposes a multi-period turning method to improve the feedrate at the junctions using the linear acceleration and deceleration mode,which utilizes the maximal acceleration capabilities of the NC machine while satisfying the machining precision.A new and more efficient look-ahead method and a feedrate override method are also proposed to boast the global machining speed.The proposed algorithm has been implemented on Blue Sky NC System,and experimented in real material manufacturing.Compared with several existing algorithms,the current algorithm can improve the manufacturing time ranging from 50% to 180%,depending on the machining parameters,and also results in better machining quality.In addition,the algorithm also satisfies the need of real-time interpolation.
基金supported by National Natural Science Foundation of China(No.51376019)
文摘The effects of different refrigerants on heat transfer performance of pulsating heat pipe(PHP) are investigated experimentally.The working temperature of pulsating heat pipe is kept in the range of 20℃-50℃.The startup time of the pulsating heat pipe with refrigerants can be shorter than 4 min,when heating power is in the range of 10W-100W.The startup time decreases with heating power.Thermal resistances of PHP with filling ratio 20.55% were obviously larger than those with other filling ratios.Thermal resistance of the PHP with R134a is much smaller than that with R404A and R600a.It indicates that the heat transfer ability of R134a is better.In addition,a correlation to predict thermal resistance of PHP with refrigerants was suggested.
