The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many o...The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.展开更多
针对中压配电网缺少实时量测、伪量测精度较低以及现有的动态状态估计(dynamic state estimation,DSE)方法均采用恒定系统处理状态过程噪声的问题,提出了一种基于改进自适应无迹卡尔曼滤波(unscented kalman filter,UKF)算法的中压配电...针对中压配电网缺少实时量测、伪量测精度较低以及现有的动态状态估计(dynamic state estimation,DSE)方法均采用恒定系统处理状态过程噪声的问题,提出了一种基于改进自适应无迹卡尔曼滤波(unscented kalman filter,UKF)算法的中压配电网鲁棒DSE方法。首先,利用中压配电网变压器低压侧的智能电表量测和变压器模型,推导出等效中压量测以增强中压配网量测冗余度;然后,借鉴信号处理技术对系统状态过程噪声的协方差矩阵实时更新并融入UKF算法,以减轻状态预测和量测滤波的不确定性;最后,基于15节点中压配电网进行仿真。仿真结果表明:所提方法能够有效地进行中压配电网的动态状态估计,获取更为精确的态势感知信息。展开更多
The monitoring of soil moisture content in paddy field is one of important parts and contents of regional soil moisture monitoring. But a good monitoring scheme hasn’t been established. A real-time monitoring scheme ...The monitoring of soil moisture content in paddy field is one of important parts and contents of regional soil moisture monitoring. But a good monitoring scheme hasn’t been established. A real-time monitoring scheme of soil moisture content in paddy field was put forward from two key links of soil moisture content monitoring and field water-layer monitoring. This scheme could meet the alternative monitoring requirements of soil moisture content in water layer and none-water layer. It had a good maneuverability and could provide references for practical work.展开更多
[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 (SS2) by fluorescent quantitative PCR. []V...[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 (SS2) by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients (FF) of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.展开更多
The trusted network connection is a hot spot in trusted computing field and the trust measurement and access control technology are used to deal with network security threats in trusted network.But the trusted network...The trusted network connection is a hot spot in trusted computing field and the trust measurement and access control technology are used to deal with network security threats in trusted network.But the trusted network connection lacks fine-grained states and real-time measurement support for the client and the authentication mechanism is difficult to apply in the trusted network connection,it is easy to cause the loss of identity privacy.In order to solve the abovedescribed problems,this paper presents a trust measurement scheme suitable for clients in the trusted network,the scheme integrates the following attributes such as authentication mechanism,state measurement,and real-time state measurement and so on,and based on the authentication mechanism and the initial state measurement,the scheme uses the realtime state measurement as the core method to complete the trust measurement for the client.This scheme presented in this paper supports both static and dynamic measurements.Overall,the characteristics of this scheme such as fine granularity,dynamic,real-time state measurement make it possible to make more fine-grained security policy and therefore it overcomes inadequacies existing in the current trusted network connection.展开更多
In this study,the micro-failure process and failure mechanism of a typical brittle rock under uniaxial compression are investigated via continuous real-time measurement of wave velocities.The experimental results indi...In this study,the micro-failure process and failure mechanism of a typical brittle rock under uniaxial compression are investigated via continuous real-time measurement of wave velocities.The experimental results indicate that the evolutions of wave velocities became progressively anisotropic under uniaxial loading due to the direction-dependent development of micro-damage.A wave velocity model considering the inner anisotropic crack evolution is proposed to accurately describe the variations of wave velocities during uniaxial compression testing.Based on which,the effective elastic parameters are inferred by a transverse isotropic constitutive model,and the evolutions of the crack density are inversed using a self-consistent damage model.It is found that the propagation of axial cracks dominates the failure process of brittle rock under uniaxial loading and oblique shear cracks develop with the appearance of macrocrack.展开更多
Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry. Therefore, a rapid, reproducible, and sensitive method for detection and quantification of this pathogen is needed ur...Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry. Therefore, a rapid, reproducible, and sensitive method for detection and quantification of this pathogen is needed urgently. To achieve this purpose, we developed a TaqMan-based real-time PCR assay for detection and quantification orE. tarda. The assay targets the hemolysin activator HlyB domain protein of E. tarda. Our optimized TaqMan assay is capable of detecting as little as 40 fg of genomic DNA per reaction. A standard curve was generated from the threshold cycle values (y) against log10 (E. tarda genomic DNA concentration) as x. The intra- and inter-assay coefficient of variation (CV) values were less than 2.06% and 1.05% respectively, indicating that the assay had good reproducibility. This method is highly specific to E. tarda strains, as it shows no cross-reactivity to Edwardsiella ictaluri, a member of the same genus, or to nine other fish-pathogenic bacteria species belonging to three other genera. This sensitive and specific real-time PCR assay provides a valuable tool for diagnostic quantitation of E. tarda in clinical samples.展开更多
Speckle interferometry is an efficient method to analyze a vibration. In certain conditions, this technique has some outstanding advantage, and need not strict shock--proof condition, compared with the holographic met...Speckle interferometry is an efficient method to analyze a vibration. In certain conditions, this technique has some outstanding advantage, and need not strict shock--proof condition, compared with the holographic method for measuring vibration. Therefore,it is suitable to analyze a vibration with a large amplitude.Real-time interferometry is a rapid and simple method for measuring vibration of a body, gives speckle pattern containing amplitude distribution of body-surface. By means of time-averaged method, the speckle pattern is recorded in Fourier transform plane, or vibration lines are seen directly with eyes, so as to analyze efficiently amplitude, phase, and model of a vibration. This paper deduces the intensity distribution function with real-time method, and gives experimental demonstration of vibration body-the vibration lines with different frequencies.展开更多
A novel data acquisition system was successfully integrated on-board the Massey Ferguson 3,000 series agricultural tractors for measuring tractor-implement performance. A commercial load cell was incorporated into the...A novel data acquisition system was successfully integrated on-board the Massey Ferguson 3,000 series agricultural tractors for measuring tractor-implement performance. A commercial load cell was incorporated into the existing system for the needed tractor-implement performance, measurements. This system is capable of measuring, displaying and recording, in real-time, the tractor's theoretical travel speed, the actual travel speed, the engine speed, the fuel consumption rate, and the drawbar pull. Static calibration tests on various associated sensors for the required measurements show excellent linearity with correlation coefficients that are close to 1. The developed system was extensively and successfully field demonstrated for tractor-implement performance with offset disc harrows on dry, undisturbed loamy soils. Under these conditions, a ratio of tractor power to implement width is suggested. The data also show the existence of a linear relationship between fuel consumption per hectare and specific draught, for the 4 to 9 kN m^-1 range, which suggests the possibility of extending the American Society of Agricultural and Biological Engineers model of draught prediction to forecast fuel consumption. The configuration of the tractor-harrow combination, based on the measurement of the draught required under operational conditions, provides the manufacturers with a reliable indication of the recommended power required for each harrow model. With this type of information farmers can make decisions regarding selection of a suitable tractor-implement combination for their farms. As a consequence, there is improved tractor-harrow productivity and field efficiency.展开更多
A simple instrument for the real-time measurement of algae concentration and mapping is described. The instrument uses a pulsed short arc xenon flashlamp as the excited light sources. Both the exciting light and the f...A simple instrument for the real-time measurement of algae concentration and mapping is described. The instrument uses a pulsed short arc xenon flashlamp as the excited light sources. Both the exciting light and the fluorescence from algae chlorophyll are transmitted along a fiber bundle. The measurement sensitivity is analyzed and the experiment result is given. The instrument is practical to in-situ measurement at sea.展开更多
Taking a specific production process as an example, this paper introduces the design of multi slot and unequal temperature PID controller based on single chip microcomputer. The mathematical implementation method...Taking a specific production process as an example, this paper introduces the design of multi slot and unequal temperature PID controller based on single chip microcomputer. The mathematical implementation method of PID algorithm and the design method of the hardware and software are discussed, The principle diagram of the hardware circuit implementing the control algorithm and the features of the software possessed are also presented.展开更多
文摘The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.
文摘针对中压配电网缺少实时量测、伪量测精度较低以及现有的动态状态估计(dynamic state estimation,DSE)方法均采用恒定系统处理状态过程噪声的问题,提出了一种基于改进自适应无迹卡尔曼滤波(unscented kalman filter,UKF)算法的中压配电网鲁棒DSE方法。首先,利用中压配电网变压器低压侧的智能电表量测和变压器模型,推导出等效中压量测以增强中压配网量测冗余度;然后,借鉴信号处理技术对系统状态过程噪声的协方差矩阵实时更新并融入UKF算法,以减轻状态预测和量测滤波的不确定性;最后,基于15节点中压配电网进行仿真。仿真结果表明:所提方法能够有效地进行中压配电网的动态状态估计,获取更为精确的态势感知信息。
文摘The monitoring of soil moisture content in paddy field is one of important parts and contents of regional soil moisture monitoring. But a good monitoring scheme hasn’t been established. A real-time monitoring scheme of soil moisture content in paddy field was put forward from two key links of soil moisture content monitoring and field water-layer monitoring. This scheme could meet the alternative monitoring requirements of soil moisture content in water layer and none-water layer. It had a good maneuverability and could provide references for practical work.
基金Supported by National Natural Science Foundation of China(31072155)Natural Science Foundation of Jiangsu Province(BK2010068)+1 种基金Fund for Independent Innovation of Agricultural Science in Jiangsu Province[CX(11)2060]Special Fund for Agroscientific Research in the Public Interest(201303041)~~
文摘[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 (SS2) by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients (FF) of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.
基金ACKNOWLEDGMENT This work was supported by the National Basic Research Program of China (973 Project) (NO.2007CB311100), the National Science Foundation for Young Scholars of China (Grant No.61001091), Beijing Nature Science Foundation(No. 4122012), "next-generation broadband wireless mobile communication network" National Science and Technology major Special issue funding(No. 2012ZX03002003), Funding Program for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality of China and the key technology research and validation issue for the emergency treatment telemedicine public service platform which integrates the military and civilian and bases on the broadband wireless networks(No.2013ZX03006001-005), the issue belongs to Major national science and technology projects.
文摘The trusted network connection is a hot spot in trusted computing field and the trust measurement and access control technology are used to deal with network security threats in trusted network.But the trusted network connection lacks fine-grained states and real-time measurement support for the client and the authentication mechanism is difficult to apply in the trusted network connection,it is easy to cause the loss of identity privacy.In order to solve the abovedescribed problems,this paper presents a trust measurement scheme suitable for clients in the trusted network,the scheme integrates the following attributes such as authentication mechanism,state measurement,and real-time state measurement and so on,and based on the authentication mechanism and the initial state measurement,the scheme uses the realtime state measurement as the core method to complete the trust measurement for the client.This scheme presented in this paper supports both static and dynamic measurements.Overall,the characteristics of this scheme such as fine granularity,dynamic,real-time state measurement make it possible to make more fine-grained security policy and therefore it overcomes inadequacies existing in the current trusted network connection.
基金Projects(41502283,41772309)supported by the National Natural Science Foundation of ChinaProject(2017YFC1501302)supported by the National Key Research and Development Program of ChinaProject(2017ACA102)supported by the Major Program of Technological Innovation of Hubei Province,China。
文摘In this study,the micro-failure process and failure mechanism of a typical brittle rock under uniaxial compression are investigated via continuous real-time measurement of wave velocities.The experimental results indicate that the evolutions of wave velocities became progressively anisotropic under uniaxial loading due to the direction-dependent development of micro-damage.A wave velocity model considering the inner anisotropic crack evolution is proposed to accurately describe the variations of wave velocities during uniaxial compression testing.Based on which,the effective elastic parameters are inferred by a transverse isotropic constitutive model,and the evolutions of the crack density are inversed using a self-consistent damage model.It is found that the propagation of axial cracks dominates the failure process of brittle rock under uniaxial loading and oblique shear cracks develop with the appearance of macrocrack.
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest(No.201103034)the Construction Special Fund of Modern Agriculture and Industrial Technology Research System(No.CARS-47)
文摘Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry. Therefore, a rapid, reproducible, and sensitive method for detection and quantification of this pathogen is needed urgently. To achieve this purpose, we developed a TaqMan-based real-time PCR assay for detection and quantification orE. tarda. The assay targets the hemolysin activator HlyB domain protein of E. tarda. Our optimized TaqMan assay is capable of detecting as little as 40 fg of genomic DNA per reaction. A standard curve was generated from the threshold cycle values (y) against log10 (E. tarda genomic DNA concentration) as x. The intra- and inter-assay coefficient of variation (CV) values were less than 2.06% and 1.05% respectively, indicating that the assay had good reproducibility. This method is highly specific to E. tarda strains, as it shows no cross-reactivity to Edwardsiella ictaluri, a member of the same genus, or to nine other fish-pathogenic bacteria species belonging to three other genera. This sensitive and specific real-time PCR assay provides a valuable tool for diagnostic quantitation of E. tarda in clinical samples.
文摘Speckle interferometry is an efficient method to analyze a vibration. In certain conditions, this technique has some outstanding advantage, and need not strict shock--proof condition, compared with the holographic method for measuring vibration. Therefore,it is suitable to analyze a vibration with a large amplitude.Real-time interferometry is a rapid and simple method for measuring vibration of a body, gives speckle pattern containing amplitude distribution of body-surface. By means of time-averaged method, the speckle pattern is recorded in Fourier transform plane, or vibration lines are seen directly with eyes, so as to analyze efficiently amplitude, phase, and model of a vibration. This paper deduces the intensity distribution function with real-time method, and gives experimental demonstration of vibration body-the vibration lines with different frequencies.
文摘A novel data acquisition system was successfully integrated on-board the Massey Ferguson 3,000 series agricultural tractors for measuring tractor-implement performance. A commercial load cell was incorporated into the existing system for the needed tractor-implement performance, measurements. This system is capable of measuring, displaying and recording, in real-time, the tractor's theoretical travel speed, the actual travel speed, the engine speed, the fuel consumption rate, and the drawbar pull. Static calibration tests on various associated sensors for the required measurements show excellent linearity with correlation coefficients that are close to 1. The developed system was extensively and successfully field demonstrated for tractor-implement performance with offset disc harrows on dry, undisturbed loamy soils. Under these conditions, a ratio of tractor power to implement width is suggested. The data also show the existence of a linear relationship between fuel consumption per hectare and specific draught, for the 4 to 9 kN m^-1 range, which suggests the possibility of extending the American Society of Agricultural and Biological Engineers model of draught prediction to forecast fuel consumption. The configuration of the tractor-harrow combination, based on the measurement of the draught required under operational conditions, provides the manufacturers with a reliable indication of the recommended power required for each harrow model. With this type of information farmers can make decisions regarding selection of a suitable tractor-implement combination for their farms. As a consequence, there is improved tractor-harrow productivity and field efficiency.
文摘A simple instrument for the real-time measurement of algae concentration and mapping is described. The instrument uses a pulsed short arc xenon flashlamp as the excited light sources. Both the exciting light and the fluorescence from algae chlorophyll are transmitted along a fiber bundle. The measurement sensitivity is analyzed and the experiment result is given. The instrument is practical to in-situ measurement at sea.
文摘Taking a specific production process as an example, this paper introduces the design of multi slot and unequal temperature PID controller based on single chip microcomputer. The mathematical implementation method of PID algorithm and the design method of the hardware and software are discussed, The principle diagram of the hardware circuit implementing the control algorithm and the features of the software possessed are also presented.