AIM: To investigate dynamical and image pathological characteristics of the liver on magnetic resonance (MR) diffusion-weighted imaging (DWI) in the rabbit VX-2 tumor model. METHODS: Forty New Zealand rabbits we...AIM: To investigate dynamical and image pathological characteristics of the liver on magnetic resonance (MR) diffusion-weighted imaging (DWI) in the rabbit VX-2 tumor model. METHODS: Forty New Zealand rabbits were included in the study and VX-2 tumor piece was implanted intrahepatically. Fifteen animals received two intrahepatic implantations while 25 had one intrahepatical implantation. DWI, T1- and T2-weighted of magnetic resonance imaging (MRI) were carried out on the 7th and the 14th d after implantation and DWI was conducted, respectively on the 21th d. Ten VX-2 tumor samples were studied pathologically. RESULTS: The rate of lump detected by DWI, TlWI and T2WI was 78.7%, 10.7% and 53.5% (X^2 =32.61, P 〈 0.001) on the 7th d after implantation and 95.8%, 54.3% and 82.9% (X^2 = 21.50, P 〈 0.001) on the 14th d. The signal of most VX-2 tumors on DWI was uniform and it was equal diffusion coefficient (ADC). did not decrease on the 7th on the map of apparent The signal of VX tumors d after implantation, most of them slowly growing during the week following implantation without significant cell dying within the tumor. VX-2 tumors grew increasingly within 14 d after implantation but the signal of most VX-2 tumors on DWI or on the map of ADC was uniform or uneven and ADC of VX tumors decreased obscurely or slightly because tumor necrosis was still not obvious. On the 21th d after implantation, the signal of most VX-2 tumors on DWI or on the map of ADC was uneven because tumor necrosis was evident and ADC of VX-2 tumor necrotic areas decreased. The areas of viable cells in VX-2 tumors manifested a high signal on DWI and a low signal on the map of ADC. The areas of dead cells or necrosis in VX-2 tumors manifested low signals on DWI and low, equal or high signals on the map of ADC but they manifested high signals on DWI and on the map of ADC at the same time when the areas of necrotic tumor became liquefied or cystic. The border of tumors on DWI appeared gradually distinct and internal signals of tumor became progressively uneven. CONCLUSION: The manifestations of viable, necrotic and liquefied or cystic areas in VX-2 tumors on DWI are typical and DWI is of significant and potential values in clinical application in both the early detection and diagnosis of liver tumors.展开更多
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multi...The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.展开更多
The casing damage has been a big problem in oilfield production. The current detection methods mostly are used after casing damage, which is not very effective. With the rapid development of China's offshore oil i...The casing damage has been a big problem in oilfield production. The current detection methods mostly are used after casing damage, which is not very effective. With the rapid development of China's offshore oil industry, the number of offshore oil wells is becoming larger and larger. Because the cost of offshore oil well is very high, the casing damage will cause huge economic losses. What's more, it can also bring serious pollution to marine environment. So the effective methods of detecting casing damage are required badly. The accumulation of stress is the main reason for the casing damage. Magnetic anisotropy technique based on counter magnetostriction effect can detect the stress of casing in real time and help us to find out the hidden dangers in time. It is essential for us to prevent the casing damage from occurring. However, such technique is still in the development stage. Previous studies mostly got the relationship between stress and magnetic signals by physical experiment, and the study of physical mechanism in relative magnetic permeability connecting the stress and magnetic signals is rarely reported. The present paper uses the ANSYS to do the three-dimensional finite element numerical simulation to study how the relative magnetic permeability works for the oil casing model. We find that the quantitative relationship between the stress' s variation and magnetic induction intensity's variation is: Δδ =K* ΔB, K = 8.04×109, which is proved correct by physical experiment.展开更多
AIM:To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach. METHODS:High-throughput NCBI blast (E-valu...AIM:To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach. METHODS:High-throughput NCBI blast (E-value cutoff e-5) was used to search homologous genes among all sequenced prokaryotic genomes of each gene encoded in each of the three strains of STEC O157:H7 with complete genomes,aiming to find unique genes in O157:H7 as its potential markers. To ensure that the identified markers from the three strains of STEC O157:H7 can serve as general markers for all the STEC O157:H7 strains,a genomic barcode approach was used to select the markers to minimize the possibility of choosing a marker gene as part of a transposable element. Effectiveness of the markers predicted was then validated by running polymerase chain reaction (PCR) on 18 strains of O157:H7 with 5 additional genomes used as negative controls. RESULTS:The blast search identified 20,16 and 20 genes,respectively,in the three sequenced strains of STEC O157:H7,which had no homologs in any of the other prokaryotic genomes. Three genes,wzy,Z0372 and Z0344,common to the three gene lists,were selected based on the genomic barcode approach. PCR showed an identification accuracy of 100% on the 18 tested strains and the 5 controls. CONCLUSION:The three identified novel markers,wzy,Z0372 and Z0344,are highly promising for the detection of STEC O157:H7,in complementary to the known markers.展开更多
OBJECTIVE To evaluate the potential efficacy of low-intensity ultrasound (US) in combination with anticancer drugs to reverse multidrug resistance (MDR)in nude mice. METHODS A total of 40 male and female athymic n...OBJECTIVE To evaluate the potential efficacy of low-intensity ultrasound (US) in combination with anticancer drugs to reverse multidrug resistance (MDR)in nude mice. METHODS A total of 40 male and female athymic nude mice were inoculated subcutaneously with 5×10^6 HepG2/ADM and HepG2 cells. Ultrasound with pulsed irradiation at an average intensity of 0.5 W/cm^2 was given to the tumor area 10 rain after administration of adriamycin (ADM). The tumor 3 dimensional diameters were measured by calipers before and after treatment, and the tumor growth indexes (TGI) calculated. RT-PCR was used to detect the gene levels of the HepG2/ADM cells. Immunohistochemical analyses for MDR proteins were conducted on the tumor tissues. RESULTS The ultrasonic treatment resulted in an average reduction in the tumor volume of 62% one month later. The relative mRNA levels of MDR1 and MRP were significantly different among the following 4 groups: untreated group as control, ADM treated; US treated; and ADM plus US treated. The mRNA levels of mdrl and mrp were down-regulated in the US groups compared to those of the non-ultrasound groups by multiple comparisons. The relative mRNA levels of Irp expression were not significantly changed. The results of immunohistochemistry indicated that tumor tissue from animals treated with US had remarkably low mdrl and mrp expression. CONCLUSION The results showed that low-intensity US can effectively reduce the size of adriamycin-resistant human hepotacarcinoma in a nude mouse model, and support the efficacy of US to overcome multiple mechanisms of drug resistance.展开更多
Sea cucumber(Apostichopus japonicus) is one of the most important aquaculture animals in China. Usually its normal body color is black that fits its living environment. The juvenile individuals obtained by crossing al...Sea cucumber(Apostichopus japonicus) is one of the most important aquaculture animals in China. Usually its normal body color is black that fits its living environment. The juvenile individuals obtained by crossing albino sea cucumber segregated in body color. To document the transcriptome difference between albino associating sea cucumber and the control, we sequenced their transcriptomes with RNA-seq. Approximately, 4.790 million(M) and 4.884 M reads, 200 nt in length, were generated from the body wall of albino associating sea cucumber and the control, respectively, from them, 9550(46.81%) putative genes were identified. In total, 583 genes were found to express differentially between albino associating sea cucumber and the control. Of these differentially expressed genes(DEGs), 4.8% changed more than five-folds. The expression levels of eight DEGs were confirmed with real-time PCR. The changing trend of these DEGs detected with real-time PCR agreed well with that detected with RNA-seq, although the change degree of some DEGs was different. Four significantly enriched pathways were identified for DEGs, which included phagocytosis, Staphylococcus aureus infection, ECM-receptor interaction and focal adhesion. These pathways were helpful for understanding the physiological difference between albino associating sea cucumber and the control.展开更多
The present researches mentioned questions of economic use of domestic triticale and their influences on a phytosanitory condition and fertility of an irrigated arable land in the Chuy Valley of Kyrgyzstan. The result...The present researches mentioned questions of economic use of domestic triticale and their influences on a phytosanitory condition and fertility of an irrigated arable land in the Chuy Valley of Kyrgyzstan. The result of the work consists of four tables, purpose of the work was to discover roots systems, nutrion value and the main laboratory testing of samples were conducted at the Humboldt University in Berlin, which was completed under the high and compliant standards of the world. The present research conducted on triticale varieties was firstly studied in Kyrgyzstan.展开更多
PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initial...PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented.展开更多
Many studies have shown that either the nearby astrophysical source or dark matter (DM) annihilation/decay can be used to explain the excess of high energy cosmic ray (CR) e^±which is detected by many experim...Many studies have shown that either the nearby astrophysical source or dark matter (DM) annihilation/decay can be used to explain the excess of high energy cosmic ray (CR) e^±which is detected by many experiments, such as PAMELA and AMS-02. Recently, the dark matter particle explorer (DAMPE) collaboration has reported its first result of the total CR e^± spectrum from 25 GeV to 4.6TeV with high precision. In this work, we study the DM annihilation and pulsar interpretations of this result. We show that the leptonic DM annihilation channels to r+'/"-, 4p, 4"/', and mixed charged lepton final states can well explain the DAMPE e^± spectrum. We also find that the mixed charged leptons channel would lead to a sharp drop structure at - TeV. However, the ordinary DM explanations have been almost excluded by the constraints from the observations of gamma-ray and CMB, unless some exotic DM models are introduced. In the pulsar scenario, we analyze 21 nearby known pulsars and assume that one of them dominantly contributes to the high energy CR e^± spectrum. Involving the constraint from the Fermi-LAT observation of the e^± anisotropy, we find that two pulsars could explain the DAMPE e^± spectrum. Our results show that it is difficult to discriminate between the DM annihilation and single pulsar explanations of high energy e^± with the current DAMPE result.展开更多
基金The National Natural Science Foundation of China, No. 30070235, 30470508The Natural Science Foundation of Hunan Province, No. 08JJ5043+1 种基金The Science and Technolgy Foundation of Hunan Province, No. 06FJ3120, 2007SK3072the Medical Science and Technology Foundation of Hunan Province, No. B2006-159
文摘AIM: To investigate dynamical and image pathological characteristics of the liver on magnetic resonance (MR) diffusion-weighted imaging (DWI) in the rabbit VX-2 tumor model. METHODS: Forty New Zealand rabbits were included in the study and VX-2 tumor piece was implanted intrahepatically. Fifteen animals received two intrahepatic implantations while 25 had one intrahepatical implantation. DWI, T1- and T2-weighted of magnetic resonance imaging (MRI) were carried out on the 7th and the 14th d after implantation and DWI was conducted, respectively on the 21th d. Ten VX-2 tumor samples were studied pathologically. RESULTS: The rate of lump detected by DWI, TlWI and T2WI was 78.7%, 10.7% and 53.5% (X^2 =32.61, P 〈 0.001) on the 7th d after implantation and 95.8%, 54.3% and 82.9% (X^2 = 21.50, P 〈 0.001) on the 14th d. The signal of most VX-2 tumors on DWI was uniform and it was equal diffusion coefficient (ADC). did not decrease on the 7th on the map of apparent The signal of VX tumors d after implantation, most of them slowly growing during the week following implantation without significant cell dying within the tumor. VX-2 tumors grew increasingly within 14 d after implantation but the signal of most VX-2 tumors on DWI or on the map of ADC was uniform or uneven and ADC of VX tumors decreased obscurely or slightly because tumor necrosis was still not obvious. On the 21th d after implantation, the signal of most VX-2 tumors on DWI or on the map of ADC was uneven because tumor necrosis was evident and ADC of VX-2 tumor necrotic areas decreased. The areas of viable cells in VX-2 tumors manifested a high signal on DWI and a low signal on the map of ADC. The areas of dead cells or necrosis in VX-2 tumors manifested low signals on DWI and low, equal or high signals on the map of ADC but they manifested high signals on DWI and on the map of ADC at the same time when the areas of necrotic tumor became liquefied or cystic. The border of tumors on DWI appeared gradually distinct and internal signals of tumor became progressively uneven. CONCLUSION: The manifestations of viable, necrotic and liquefied or cystic areas in VX-2 tumors on DWI are typical and DWI is of significant and potential values in clinical application in both the early detection and diagnosis of liver tumors.
基金Project (Nos. 2003C13015 and 021103128) supported by Scienceand Technology Department of Zhejiang Province, China
文摘The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.
基金supported by the National Natural Science Foundation of China(No.41174157)
文摘The casing damage has been a big problem in oilfield production. The current detection methods mostly are used after casing damage, which is not very effective. With the rapid development of China's offshore oil industry, the number of offshore oil wells is becoming larger and larger. Because the cost of offshore oil well is very high, the casing damage will cause huge economic losses. What's more, it can also bring serious pollution to marine environment. So the effective methods of detecting casing damage are required badly. The accumulation of stress is the main reason for the casing damage. Magnetic anisotropy technique based on counter magnetostriction effect can detect the stress of casing in real time and help us to find out the hidden dangers in time. It is essential for us to prevent the casing damage from occurring. However, such technique is still in the development stage. Previous studies mostly got the relationship between stress and magnetic signals by physical experiment, and the study of physical mechanism in relative magnetic permeability connecting the stress and magnetic signals is rarely reported. The present paper uses the ANSYS to do the three-dimensional finite element numerical simulation to study how the relative magnetic permeability works for the oil casing model. We find that the quantitative relationship between the stress' s variation and magnetic induction intensity's variation is: Δδ =K* ΔB, K = 8.04×109, which is proved correct by physical experiment.
基金Supported by National Natural Science Foundation of China, No. 30872415National Science Foundation, No. DBI-0354771, ITR-IIS-0407204, DBI-0542119, CCF0621700+1 种基金National Institutes of Health, No. 1R01GM075331 and 1R01GM081682BioEnergy Science Center, US Department of Energy BioEnergy Research Center supported by the Office of Biological and Environmental Research in DOE Office of Science
文摘AIM:To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach. METHODS:High-throughput NCBI blast (E-value cutoff e-5) was used to search homologous genes among all sequenced prokaryotic genomes of each gene encoded in each of the three strains of STEC O157:H7 with complete genomes,aiming to find unique genes in O157:H7 as its potential markers. To ensure that the identified markers from the three strains of STEC O157:H7 can serve as general markers for all the STEC O157:H7 strains,a genomic barcode approach was used to select the markers to minimize the possibility of choosing a marker gene as part of a transposable element. Effectiveness of the markers predicted was then validated by running polymerase chain reaction (PCR) on 18 strains of O157:H7 with 5 additional genomes used as negative controls. RESULTS:The blast search identified 20,16 and 20 genes,respectively,in the three sequenced strains of STEC O157:H7,which had no homologs in any of the other prokaryotic genomes. Three genes,wzy,Z0372 and Z0344,common to the three gene lists,were selected based on the genomic barcode approach. PCR showed an identification accuracy of 100% on the 18 tested strains and the 5 controls. CONCLUSION:The three identified novel markers,wzy,Z0372 and Z0344,are highly promising for the detection of STEC O157:H7,in complementary to the known markers.
基金This work was supported by a grant from theNational Natural Science Foundation of China(No.30200060).
文摘OBJECTIVE To evaluate the potential efficacy of low-intensity ultrasound (US) in combination with anticancer drugs to reverse multidrug resistance (MDR)in nude mice. METHODS A total of 40 male and female athymic nude mice were inoculated subcutaneously with 5×10^6 HepG2/ADM and HepG2 cells. Ultrasound with pulsed irradiation at an average intensity of 0.5 W/cm^2 was given to the tumor area 10 rain after administration of adriamycin (ADM). The tumor 3 dimensional diameters were measured by calipers before and after treatment, and the tumor growth indexes (TGI) calculated. RT-PCR was used to detect the gene levels of the HepG2/ADM cells. Immunohistochemical analyses for MDR proteins were conducted on the tumor tissues. RESULTS The ultrasonic treatment resulted in an average reduction in the tumor volume of 62% one month later. The relative mRNA levels of MDR1 and MRP were significantly different among the following 4 groups: untreated group as control, ADM treated; US treated; and ADM plus US treated. The mRNA levels of mdrl and mrp were down-regulated in the US groups compared to those of the non-ultrasound groups by multiple comparisons. The relative mRNA levels of Irp expression were not significantly changed. The results of immunohistochemistry indicated that tumor tissue from animals treated with US had remarkably low mdrl and mrp expression. CONCLUSION The results showed that low-intensity US can effectively reduce the size of adriamycin-resistant human hepotacarcinoma in a nude mouse model, and support the efficacy of US to overcome multiple mechanisms of drug resistance.
基金funded by the National Natural Science Foundation of China (No. 40976089)the National Key Technology Support Program of China (No. 2011BAD13B02)+1 种基金the National Oceanic Public Welfare Industry Special Scientific Research of China (No. 201205023)the Chinese National 863 Project (2012AA10A412)
文摘Sea cucumber(Apostichopus japonicus) is one of the most important aquaculture animals in China. Usually its normal body color is black that fits its living environment. The juvenile individuals obtained by crossing albino sea cucumber segregated in body color. To document the transcriptome difference between albino associating sea cucumber and the control, we sequenced their transcriptomes with RNA-seq. Approximately, 4.790 million(M) and 4.884 M reads, 200 nt in length, were generated from the body wall of albino associating sea cucumber and the control, respectively, from them, 9550(46.81%) putative genes were identified. In total, 583 genes were found to express differentially between albino associating sea cucumber and the control. Of these differentially expressed genes(DEGs), 4.8% changed more than five-folds. The expression levels of eight DEGs were confirmed with real-time PCR. The changing trend of these DEGs detected with real-time PCR agreed well with that detected with RNA-seq, although the change degree of some DEGs was different. Four significantly enriched pathways were identified for DEGs, which included phagocytosis, Staphylococcus aureus infection, ECM-receptor interaction and focal adhesion. These pathways were helpful for understanding the physiological difference between albino associating sea cucumber and the control.
文摘The present researches mentioned questions of economic use of domestic triticale and their influences on a phytosanitory condition and fertility of an irrigated arable land in the Chuy Valley of Kyrgyzstan. The result of the work consists of four tables, purpose of the work was to discover roots systems, nutrion value and the main laboratory testing of samples were conducted at the Humboldt University in Berlin, which was completed under the high and compliant standards of the world. The present research conducted on triticale varieties was firstly studied in Kyrgyzstan.
基金supported by a 985 grant from Shanghai Jiao Tong Universitythe National Basic Research Program of China (Grant No. 2010CB833005)+4 种基金the National Natural Science Foundation of China (Grant No. 11055003)the Office of Science and Technology in Shanghai Municipal Government (Grant No. 11DZ2260700)sponsored by Shandong University, Peking Universitythe University of Marylandthe University of Michigan
文摘PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented.
基金supported by the National Key Program for Research and Development(Grant No.2016YFA0400200)the National Natural Science Foundation of China(Grant Nos.U1738209,11475189,and 11475191)
文摘Many studies have shown that either the nearby astrophysical source or dark matter (DM) annihilation/decay can be used to explain the excess of high energy cosmic ray (CR) e^±which is detected by many experiments, such as PAMELA and AMS-02. Recently, the dark matter particle explorer (DAMPE) collaboration has reported its first result of the total CR e^± spectrum from 25 GeV to 4.6TeV with high precision. In this work, we study the DM annihilation and pulsar interpretations of this result. We show that the leptonic DM annihilation channels to r+'/"-, 4p, 4"/', and mixed charged lepton final states can well explain the DAMPE e^± spectrum. We also find that the mixed charged leptons channel would lead to a sharp drop structure at - TeV. However, the ordinary DM explanations have been almost excluded by the constraints from the observations of gamma-ray and CMB, unless some exotic DM models are introduced. In the pulsar scenario, we analyze 21 nearby known pulsars and assume that one of them dominantly contributes to the high energy CR e^± spectrum. Involving the constraint from the Fermi-LAT observation of the e^± anisotropy, we find that two pulsars could explain the DAMPE e^± spectrum. Our results show that it is difficult to discriminate between the DM annihilation and single pulsar explanations of high energy e^± with the current DAMPE result.