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邓恩桉芽器官离体诱导培养影响因素的研究 被引量:5
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作者 欧阳磊 林彦 +2 位作者 刘友全 谢耀坚 曹月华 《桉树科技》 2003年第2期53-58,共6页
初步研究了以邓恩桉优选实生种子苗的带芽茎段作为外植体诱导丛生芽发生和生长的过程。对培养过程中丛生芽的发生和生长情况进行了探讨。通过正交试验和单因子试验,确定了邓恩桉丛生芽诱导的最适培养条件:(1)基本培养基:1/2MS+BA0.5mg&#... 初步研究了以邓恩桉优选实生种子苗的带芽茎段作为外植体诱导丛生芽发生和生长的过程。对培养过程中丛生芽的发生和生长情况进行了探讨。通过正交试验和单因子试验,确定了邓恩桉丛生芽诱导的最适培养条件:(1)基本培养基:1/2MS+BA0.5mg·L^(-1)+IAA0.1mg·L^(-1)+3%蔗糖+1.1%卡拉胶;(2)培养温度:24℃~28 ℃;(3)光照条件:暗培养7-10天后转到自然散射光较强的光照条件下培养。 展开更多
关键词 邓恩桉 芽器官 离体诱导培养 实生种子苗 带芽茎段 外植体 丛生芽 发生 生长 培养条件 培养基 温度 光照
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Methods of Testing Seed and Seedling Physiological Traits for the Improvement of Rapeseed Yield Stability
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作者 Ladislav Blaiha Dagmar Janovska Miroslava Vyvadilova 《Journal of Life Sciences》 2014年第2期152-163,共12页
The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It al... The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It allows one to eliminate the plant materials (initial breeding materials and cultivars) which do not tolerate extreme temperatures and temperature changes during germination, have low water use efficiency and is intolerant to abiotic stressors all right at the seed level. It was confirmed that these genotypes also have poor t^eld emergence and initial root growth implications for further vegetation periods, mainly for over wintering and spring regeneration which has significant influence on the yield. The method also represents the tool for screening genetic resources with the resistance to the abiotic stressors and this technology process is also acceptable for other crops. The results confirm the importance of the seed and root characteristics for crop production. The deteriorating quality of soil in recent years, increasing variability of weather and long periods of drought directly support the need to intensify activities in this research. Obtained results also show the influence of seed quality characteristics on roots and above ground parts of the plant. A relationship exists also vice versa. 展开更多
关键词 Seed traits root traits PROVENANCE periodicity of germination.
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