实色面漆循环系统再次清洗注意事项

针对实色面漆循环系统在长时间工作后或更换颜色时,循环系统需要清洗的情况,介绍了清洗前准备、罐体清洗、循环泵清洗、循环系统清洗时的注意事项。

中密度纤维板的实色UV辊涂工艺分析

对中密度纤维板实色UV辊涂生产工艺及设备配置进行分析,提出中密度纤维板实色UV辊涂适宜工艺为两道腻子填充、三道底漆及两道面漆共七道涂布工序。经验证试验,涂饰板材的漆膜性能优良。

电脑测配色系统在实色涂料中的应用

简要介绍电脑测配色系统中的硬件和软件系统中的概念,详细阐述了应用于atacolor电脑测配色系统建立配色数据库的过程,及影响电脑测配色系统在实色涂料中颜色精度的因素。

客车实色面漆色差影响因素的分析与预防

客车的颜色和图案以定制化生产为主,种类繁多,经常容易出现色差问题。客车实色面漆色差产生的原因主要有两个方面:一是涂料本身颜色与标准颜色之间的差异;二是面漆涂装施工过程中造成的颜色变化。本文结合客车面漆生产和涂装的具体工艺过程,分析了色差产生的主要影响因素,提出了控制和预防措施。

汽车用水性实色白面漆外观优化的研究

在深入探究水性汽车涂料外观形成机理特别是水性实色白面漆配方特殊性的基础上,通过一种固定的研究模式:从外观机理研究,到确定外观影响因素,到配方设计改变影响因素,到物理化学性能分析确定影响因素变量,再到喷涂应用试验确认外观的优化。可靠地验证了两种外观形成的可能机理,同时为客户涂装现场的外观改进提供了配方优化的方案。

经济型双组分实色木器涂料耐黄产品的制备

通过对涂膜降解的原理分析,从树脂耐黄变性、耐黄变助剂的耐黄变性、固化剂的耐黄变性进行试验、分析、选择。最终确定了以椰子油改性醇酸树脂为基础,耐黄变助剂选用紫外线吸收剂与受阻胺自由基捕捉剂搭配使用,固化剂选用TDI三聚体搭配HDI三聚体进行制作。同时探讨了制作能够满足国标对耐黄变性要求的产品的制作方法。

实色聚酯氨基汽车面漆的试制与应用

试制实色聚酯氨基汽车面漆,烘烤条件为140℃×30 min。分析了各原材料的选择及其影响。

Comparative study of Chinese and English color words

There are a lot of color words in both English and Chinese. The color words in both English and Chinese can be divided into two parts： basic color words and real-stuff based color words. In this paper the formation, syntactic functions and characteristics of English and Chinese color words have been analytically studied, and some differences and similarities of English and Chinese color words have been summed up.

Pharmacokinetics of Scutellarin in Dogs

Aim To establish an RP-HPLC method for the determination of scutellarin in plasma and study its pharmacokinetics in dogs. Methods Scutellarin was given to dogs by intravenous injection and determined by RP-HPLC, the mean plasma concentration-time curve was plotted and pharmacokinetic parameters were calculated by program 3p87. Resu;ts The concentration-time curve of scutellarin could be fitted to three-compartment model with T1/2 pi, T1/2 α and T1/2 β being 1.05 ± 0.80 min, 6.99 + 2.76 min and 51.61 + 28.78 min, respectively, Vc being 880.1 + 508.3 mL, CL being 189.6 + 53.8 mL@ min- 1, and AUC0-90 and AUC0-∞ being 574.43 + 133.95 μg@ min@ mL - 1 and 599.34 ± 132.00μg@ min@mL- 1, respectively. Conclusion The fact that the concentrations of scutellarin in plasma declined rapidly after the medication suggested that the T1/2 of scutellarin should be taken into account in drug administration and preparation development.

唯识学对五法三身的安立——以三部唯识论著为中心

《摄大乘论》将佛身开显为法身、受用身和变化身,指出四智能现受用、变化二身,大圆镜智能现受用身,成所作智能现变化身,平等性智和妙观察智亦能现起佛身,但没有明言具体能现何种佛身。《佛地经论》不仅提出五法摄三身的佛身思想,还用两种佛身观进一步细化五法与三身相互摄持的关系。这两种佛身观是《成唯识论》佛身思想的渊薮,但《成唯识论》对五法与三身的相摄关系有更细致的分化:在第二种佛身观中,将四智及其所摄自受用身、他受用身和变化身统归于假名有,唯有清净真如及其所摄自性身为真实有,从而开显出更为周密的身智关系。这种佛身观比另一种佛身观的内容更丰富,在理论构架上更加清晰,而且对法相的破除也更彻底,有助于唯识学佛身观的深层构建。

Chemical Compositions of Volatile Oil from Fruiting Body of Armillaria luteo-virens

The chemical compositions of volatile oil from fruiting body of Armillaria luteo-virens in Qinghai Province were firstly analyzed with GC-MS and its relevant compositions were detected by calculating chromatographic peak area with normalized method. 21 peaks were separated and 13 compositions were identified which were mainly unsaturated fatty acids, taking 97.1% of the total volatile oil.

Experimental investigation on the energy evolution of dry and water-saturated red sandstones

In order to investigate the effect of water content on the energy evolution of red sandstone, the axial loading–unloading experiments on dry and water-saturated sandstone samples were conducted, and the distribution and evolution of elastic energy and dissipated energy within the rock were measured.The results show that the saturation process from dry to fully-saturated states reduces the strength, rigidity and brittleness of the rock by 30.2%, 25.5% and 16.7%, respectively. The water-saturated sample has larger irreversible deformation in the pre-peak stage and smaller stress drop in the post-peak stage.The saturation process decreases the accumulation energy limit by 38.9%, but increases the dissipated energy and residual elastic energy density, thus greatly reducing the magnitude and rate of energy release. The water-saturated sample has lower conversion efficiency to elastic energy by 3% in the prepeak region; moreover, the elastic energy ratio falls with a smaller range in the post-peak stage.Therefore, saturation process can greatly reduce the risk of dynamic disaster, and heterogeneous water content can lead to dynamic disaster possibly on the other hand.

Protective effect of exogenous adenosine triphosphate on hypothermically preserved rat liver

AIM:To clarify the protective effect of exogenous adenosine triphosphate(ATP)on hypothermically preserved rat livers. METHODS:Establishment of continuous hypothermic machine perfusion model,detection of nucleotides in hepatocytes with HPLC,measurement of activities of LDH and AST in the perfusate,observation of histopathological changes in different experiment groups,and autoradiography were carried out to reveal the underlying mechanism of the protective effect of ATP. RESULTS:The intracellular levels of ATP and EC decreased rapidly after hypothermic preservation in control group,while a higher ATP and EC level,and a slower decreasing rate were observed when ATP-MgCl_2 was added to the perfusate (P<0.01).As compared with the control group,the activities of LDH and AST in the ATP-MgCl_2 group were lower(P<0.05). Furthermore,more severe hepatocyte damage and neutrophil infiltration were observed in the control group.Radioactive [α-^(32)P]ATP entered the hypothermically preserved rat hepatocytes. CONCLUSION:Exogenous ATP has a protective effect on rat livers during hypothermical preservation.However,Mg^(2+) is indispensable,addition of ATP alone produces no protective effect.The underlying mechanism may be that exogenous ATP enters the hypothermically preserved rat liver cells.

Yamanaka factors critically regulate the developmental signaling network in mouse embryonic stem cells

Yamanaka factors （Oct3/4, Sox2, Klf4, c-Myc） are highly expressed in embryonic stem （ES） cells, and their overexpression can induce pluripotency in both mouse and human somatic cells, indicating that these factors regulate the developmental signaling network necessary for ES cell pluripotency. However, systemic analysis of the signaling pathways regulated by Yamanaka factors has not yet been fully described. In this study, we identified the target promoters of endogenous Yamanaka factors on a whole genome scale using ChIP （chromatin immunoprecipitation）- on-chip in El4.1 mouse ES cells, and we found that these four factors co-occupied 58 promoters. Interestingly, when Oct4 and Sox2 were analyzed as core factors, Klf4 functioned to enhance the core factors for development regulation, whereas c-Myc seemed to play a distinct role in regulating metabolism. The pathway analysis revealed that Yamanaka factors collectively regulate a developmental signaling network composed of 16 developmental signaling pathways, nine of which represent earlier unknown pathways in ES cells, including apoptosis and cellcycle pathways. We further analyzed data from a recent study examining Yamanaka factors in mouse ES ceils. Interestingly, this analysis also revealed 16 developmental signaling pathways, of which 14 pathways overlap with the ones revealed by this study, despite that the target genes and the signaling pathways regulated by each individual Yamanaka factor differ significantly between these two datasets. We suggest that Yamanaka factors critically regulate a developmental signaling network composed of approximately a dozen crucial developmental signaling pathways to maintain the pluripotency of ES cells and probably also to induce pluripotent stem cells.

Experimental Study on Dispersion Coefficient of Dredger Fill in Reclamation Region

In order to satisfy the demand of land use, reclamation engineering has been undertaken in coastal cities for a long time. To study the contaminant transport in the reclamation districts, robust and accurate estimation of dispersion coefficient is essential. In this study, the continuous sodium chloride （NaC1） solution with constant concentration was introduced into the column filled with the dredger fill to get the breakthrough curves （BTCs）. Inverse error function method （IEFM） and CXTFIT program were used for estimating dispersion coefficient. Results showed that the difference between the dispersion coefficients estimated by IEFM and CXTFIT program was slight. The main reason was that the BTC was not strictly linear. IEFM performed poorly in the nonlinear area, while the CXTFIT performed well over the entire BTCs. Moreover, the dispersion coefficient of dredger fill was small. The dispersion property of dredger fill would result in the slow migration of contaminants in the dredger fill.

Long-term modifications of blood pressure in normotensive and spontane-ously hypertensive rats by gene delivery of rAAV-mediated cytochrome P450 arachidonic acid hydroxylase

Arachidonic acid cytochrome P-450 （CYP） hydroxylase 4A isoforms, including 4A1, 4A2, 4A3 and 4A8 in the rat kidney, catalyze arachidonic acid to produce 19/20-Hydroxyeicosatetraenoic acids （20-HETE）, a biologically active metabolite, which plays an important role in the regulation of blood pressure. However, controversial results have been reported regarding the exact role of 20-HETE on blood pressure. In the present study, we used recombinant adenoassociated viral vector （rAAV） to deliver CYP 4A1 cDNA and antisense 4A1 cDNA into Sprague-Dawley （SD） rats and spontaneously hypertensive rats （SHR）, respectively, to investigate the effects of long-term modifications of blood pressure and the potential for gene therapy of hyperténsion. The mean systolic pressure increased by 14.2±2.5 mm Hg in rAAV.4A 1-treated SD rats and decreased by 13.7±2.2 mm Hg in rAAV.anti4A l-treated SHR rats 5 weeks after the injection compared with controls and these changes in blood pressure were maintained until the experiments ended at 24 weeks. In 4A1 treated animals CYP4A was overexpressed in various tissues, but preferentially in the kidney at both mRNA and protein levels. In anti-4Al-treated SHR, CYP4A mRNA in various tissues was probed, especially in kidneys, but 4A l protein expression was almost completely inhibited. These results suggest that arachidonic acid CYP hydroxylases contribute not only to the maintenance of normal blood pressure but also to the development of hypertension. rAAV-mediated anti4A administration strategy has the potential to be used as targeted gene therapy in human hypertension by blocking expression of CYP 4A in kidneys.

Chromatin-binding in vivo of the erythroid kruppel-like factor,EKLF,in the murine globin loci

EKLF is an erythroid-specific, zinc finger-containing transcription factor essential for the activation of the mammalian beta globin gene in erythroid cells of definitive lineage. We have prepared a polyclonal anti-mouse EKLF antibody suitable for Western blotting and immunoprecipitation （IP） qualities, and used it to define the expression patterns of the EKLF protein during mouse erythroid development. We have also used this antibody for the chromatin-immunoprecipitation （CHIP） assay. EKLF was found to bind in vivo at both the mouse beta-major-globin promoter and the HS2 site of beta-LCR in the mouse erythroleukemia cells （MEL） in a DMSO-inducible manner. The DMSO-induced bindings of EKLF as well as three other proteins, namely, RNA polymerase Ⅱ, acetylated histone H3, and methylated histone H3, were not abolished but significantly lowered in CB3, a MEL-derived cell line with null-expression of p45/NF-E2, an erythroid-enriched factor needed for activation of the mammalian globin loci. Interestingly, binding of EKLF in vivo was also detected in the mouse alpha-like globin locus, at the adult alpha globin promoter and its far upstream regulatory element alpha-MRE （HS26）. This study provides direct evidence for EKLF-binding in vivo at the major regulatory elements of the mouse beta-like globin gene clusters the data also have interesting implications with respect to the role of EKLF-chromatin interaction in mammalian globin gene regulation.

Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes

AIM： To investigate the possible mechanism for HBV X gene to induce apoptosis of hepatocyte HL-7702 cells.METHODS： HBV X gene eukaryon expression vector pcDNA3-X was established and transfected into HL-7702 cells by lipid-mediated transfection, including transient and stable transfection. Positive clones were screened by incubating in the selective medium with 600 μg/mL G418 and named HL-7702/HBV-encoded X protein （HBx） cells. The expressions of Fas/FasL, Bax/Bcl-2, and c-myc mRNA were measured by semi-quantitative RT-PCR in HL-7702/HBx and control group, respectively.RESULTS： RT-PCR analysis confirmed that HBV X gene was transfected into HL-7702 cells successfully. By semiquantitative RT-PCR analysis, Bax and c-myc mRNA levels in HL-7702/HBx cells of transient transfection were significantly higher than those in control, FasL and c-myc mRNA levels in HL-7702/HBx cells of stable transfection were significantly higher than those in control, whereas the Bcl-2 mRNA levels in HL-7702/HBx cells of transient and stable transfection were significantly lower than thosein control.CONCLUSION： HBV X gene may promote the apoptosis of hepatocytes by regulating the expressions of Fas/FasL, Bax/Bcl-2, and c-myc gene in a dose-dependent manner.

A Framework for Implementation of Green Manufacturing in Customized Products Manufacturing Enterprises

An increase in public environmental awareness and pressures from the government’s determination to deal with environmental problems force manufacturers to implement green manufacturing(GM).However,since managers and stakeholders lack understanding of GM and its complexity,the manufacturing enterprise,especially the small and medium ones,are constantly facing the problems of how to make a reasonable decision for an environmental problem,and the adopted approaches have no clear payoffs.The customized design,flexible production,and diversified services in customized production make the problems more challenging.In order to solve this problem,this paper proposes a framework for the implementation of GM in customized products manufacturing enterprises(CPME).A three-layer framework,i.e.,the goal layer,the product life-cycle layer and the supporting layer,is designed to provide a methodology to help implement GM.In this model,the GM practice processes are divided into four stages from the life-cycle perspective,i.e.design,production,use,and disposal.The preliminary practice of GM in an electrical product manufacturing company is carried out,and the implementation effect shows that the system framework is helpful to make a comprehensive understanding of GM and to improve the operability of GM practices.The integrated product model is an effective way to integrate the life cycle data.

Two Kinds of Two-Boson Realizations of Generally Deformed Algebras with Three Generators

In this paper two kinds of two-boson realizations of generally deformed algebras with three generators are obtained by generalizing the Jordan-Schwlnger realizations of SU（2） and SU（1,1）. For each kind, a unitary realization and a non-unitary realizatlon, together with the properties of their acting spaces, are discussed. Similarity transformations that relate the non-unitary realizations to the unitary ones are given by solving unitarization equations.