In order to explore the reason for the frequency explosion of spontaneous combustion coal gangue dump and get to know their explosion mechanism, established the experiment platform about spontaneous combustion coal ga...In order to explore the reason for the frequency explosion of spontaneous combustion coal gangue dump and get to know their explosion mechanism, established the experiment platform about spontaneous combustion coal gangue dump interoperable water. The gangue dump was heated using the external heat source, and rainy weather through water mist was simulated. Simulated experiment about explosion of spontaneous combustion coal gangue dump was carried out on the different conditions of the rainfall or not. The unusual gases produced in the course of gangue combustion and the changes in temperature were observed, and their impacts on the explosion of gangue dump were analyzed. The experimental results show that overall warming phenomenon of the gangue dump after watering occurred, the amount of H2 is three times than that before watering, and the amount of CO is far greater than that on the conditions of no-watering, at the same time combining with local observation and tests. It is found that the content of the oxygen reduce with the temperature increasing; however, gangue dump internal hydrogen content increase unusually on the rainfall conditions at 90 ℃, but the local measuring points reach the explosion limit. The existence of the CO and H2 is the main reason for inducing the explosion of spontaneous combustion coal gangue dump.展开更多
To explore the role of nuclear factor-κB(NF-κB) in the signal pathway of protein kinase C (PKC) regulating the proliferation and apoptosis of T lymphocytes in asthma. T lymphocytes were isolated from the asthmatic m...To explore the role of nuclear factor-κB(NF-κB) in the signal pathway of protein kinase C (PKC) regulating the proliferation and apoptosis of T lymphocytes in asthma. T lymphocytes were isolated from the asthmatic model of guinea pigs and the asthmatic patients. Either the T cells stimulated with PMA alone or those stimulated with PMA together with pyrrolidine dithiocarbamate (PDTC) were incubated for 1 and 24?h. The proliferation of and the presence of NF-κB in the cells incubated for 1?h were observed by MTT and immunohistochemical staining, respectively. And the cells incubated for 24?h were observed for the apoptosis by TUNEL. All the assays were paralleled with controls, and all the data were analyzedstatistically with the software SAS. The percentage of cells of nuclear positive staining of NF-κB and the proliferation of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated with PMA were significantly higher than those of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated without PMA respectively (P<0.01) and those of T lymphocytes from normal control guinea pigs and normal control persons stimulated with PMA respectively (P<0.01), and were significantly reduced by PDTC (P<0.01). The apoptosis index of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated with PMA were significantly lower than those of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated without PMA respectively (P<0.01) and those of T lymphocytes from normal control guinea pigs and normal control persons stimulated with PMA respectively (P<0.01), and were significantly induced by PDTC (P<0.01). There were good positive correlation between the percentage of cells of nuclear staining of NF-κB of T lymphocytes and the proliferation of T lymphocytes (r=0.51-0.72, P<0.001), and also good negative correlation between the percentage of cells of nuclear staining of NF-κB and the apoptosis index of T lymphocytes (r=-0.55-0.71, P<0.001, respectively). It concludes that the active PKC of asthmatic T lymphocytes promoting the proliferation and inhibiting the apoptosis of T lymphocytes may be mediated by activating NF-κB, the activation of PKC-NF-κB signal pathway of T lymphocytes NF-κB may play an important role in the pathogenesis of asthma.展开更多
基金Supported by the National Natural Science Foundation of China(50274061)IRT (0618)Youth Fundation of Henan Polytechnic University (Q2008-33)
文摘In order to explore the reason for the frequency explosion of spontaneous combustion coal gangue dump and get to know their explosion mechanism, established the experiment platform about spontaneous combustion coal gangue dump interoperable water. The gangue dump was heated using the external heat source, and rainy weather through water mist was simulated. Simulated experiment about explosion of spontaneous combustion coal gangue dump was carried out on the different conditions of the rainfall or not. The unusual gases produced in the course of gangue combustion and the changes in temperature were observed, and their impacts on the explosion of gangue dump were analyzed. The experimental results show that overall warming phenomenon of the gangue dump after watering occurred, the amount of H2 is three times than that before watering, and the amount of CO is far greater than that on the conditions of no-watering, at the same time combining with local observation and tests. It is found that the content of the oxygen reduce with the temperature increasing; however, gangue dump internal hydrogen content increase unusually on the rainfall conditions at 90 ℃, but the local measuring points reach the explosion limit. The existence of the CO and H2 is the main reason for inducing the explosion of spontaneous combustion coal gangue dump.
文摘To explore the role of nuclear factor-κB(NF-κB) in the signal pathway of protein kinase C (PKC) regulating the proliferation and apoptosis of T lymphocytes in asthma. T lymphocytes were isolated from the asthmatic model of guinea pigs and the asthmatic patients. Either the T cells stimulated with PMA alone or those stimulated with PMA together with pyrrolidine dithiocarbamate (PDTC) were incubated for 1 and 24?h. The proliferation of and the presence of NF-κB in the cells incubated for 1?h were observed by MTT and immunohistochemical staining, respectively. And the cells incubated for 24?h were observed for the apoptosis by TUNEL. All the assays were paralleled with controls, and all the data were analyzedstatistically with the software SAS. The percentage of cells of nuclear positive staining of NF-κB and the proliferation of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated with PMA were significantly higher than those of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated without PMA respectively (P<0.01) and those of T lymphocytes from normal control guinea pigs and normal control persons stimulated with PMA respectively (P<0.01), and were significantly reduced by PDTC (P<0.01). The apoptosis index of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated with PMA were significantly lower than those of T lymphocytes from asthmatic guinea pigs and asthmatic patients stimulated without PMA respectively (P<0.01) and those of T lymphocytes from normal control guinea pigs and normal control persons stimulated with PMA respectively (P<0.01), and were significantly induced by PDTC (P<0.01). There were good positive correlation between the percentage of cells of nuclear staining of NF-κB of T lymphocytes and the proliferation of T lymphocytes (r=0.51-0.72, P<0.001), and also good negative correlation between the percentage of cells of nuclear staining of NF-κB and the apoptosis index of T lymphocytes (r=-0.55-0.71, P<0.001, respectively). It concludes that the active PKC of asthmatic T lymphocytes promoting the proliferation and inhibiting the apoptosis of T lymphocytes may be mediated by activating NF-κB, the activation of PKC-NF-κB signal pathway of T lymphocytes NF-κB may play an important role in the pathogenesis of asthma.
