OBJECTIVE： To investigate the inhibitory effect of Panax notoginseng saponins（PNS） on liver fibrosis and explore the underlying mechanisms.METHODS： Carbon tetrachloride（CCl4）-treated rats and hepatic stellate ce...OBJECTIVE： To investigate the inhibitory effect of Panax notoginseng saponins（PNS） on liver fibrosis and explore the underlying mechanisms.METHODS： Carbon tetrachloride（CCl4）-treated rats and hepatic stellate cells（HSCs） were used. The effect of PNS on CCl4-induced liver fibrosis was studied with histochemical and biochemical analysis.Transforming growth factor（TGF）-β1, α-smooth muscle actin（α-SMA）, and collagen Ι m RNA expression were determined by reverse transcriptase-polymerase chain reaction（RT-PCR）. Mean-while, the protein expression levels of α-SMA, collagen Ι, phosphorylation-Janus activated kinase signal transducer（p-Jak2） / Jak2, and phosphorylation-activator of transcription（p-Stat）3 / Stat3 were determined by immunohistochemistry and / or immunoblotting.RESULTS： PNS treatment significantly improved the liver function of rats as indicated by decreased serum enzymatic activities of alanine aminotransferase and aspartate aminotransferase. Histopathological results indicated that PNS alleviated liver damage and reduced the formation of fibrous septa. Moreover, PNS significantly decreased liver hydroxyproline and significantly attenuated expressions of collagen p-Stat3 / StatⅠ, α-SMA, TGF-β1, p-Jak2 / Jak2,and 3 in the rat liver fibrosis model and HSCs.CONCLUSION： PNS can relieve liver fibrosis by modulating Jak2/Stat3 signaling transduction pathway, which may be one of its mechanisms to suppress hepatic fibrosis.展开更多
Experimental research was conducted to understand heat transfer characteristic of pulsating heat pipe in this paper, and the PHP is made of high quality glass capillary tube. Under different fill ratio, heat transfer ...Experimental research was conducted to understand heat transfer characteristic of pulsating heat pipe in this paper, and the PHP is made of high quality glass capillary tube. Under different fill ratio, heat transfer rate and many other influence factors, the flow patterns were observed in the start-up, transition and stable stage. The effects of heating position on heat transfer were discussed. The experimental results indicate that no annular flow appears in top heating condition. Under different fall ratios and heat transfer rate, the flow pattern in PHP is transferred from bulk flow to semi-annular flow and annular flow, and the performance of heat transfer is improved for down heating case. The experimental results indicate that the total heat resistant of PHP is increased with fill ratio, and heat transfer rate achieves optimum at filling rate 50%. But for pulsating heat pipe with changing diameters the thermal resistance is higher than that with uniform diameters.展开更多
基金Supported by Natural Science Foundation of the Education Department of Anhui Province(Effect of Panax Notoginseng Saponins on the Proliferation and Apoptosis and its Regulation of Signal Transduction pathways in HSC,No.KJ2011A187)
文摘OBJECTIVE： To investigate the inhibitory effect of Panax notoginseng saponins（PNS） on liver fibrosis and explore the underlying mechanisms.METHODS： Carbon tetrachloride（CCl4）-treated rats and hepatic stellate cells（HSCs） were used. The effect of PNS on CCl4-induced liver fibrosis was studied with histochemical and biochemical analysis.Transforming growth factor（TGF）-β1, α-smooth muscle actin（α-SMA）, and collagen Ι m RNA expression were determined by reverse transcriptase-polymerase chain reaction（RT-PCR）. Mean-while, the protein expression levels of α-SMA, collagen Ι, phosphorylation-Janus activated kinase signal transducer（p-Jak2） / Jak2, and phosphorylation-activator of transcription（p-Stat）3 / Stat3 were determined by immunohistochemistry and / or immunoblotting.RESULTS： PNS treatment significantly improved the liver function of rats as indicated by decreased serum enzymatic activities of alanine aminotransferase and aspartate aminotransferase. Histopathological results indicated that PNS alleviated liver damage and reduced the formation of fibrous septa. Moreover, PNS significantly decreased liver hydroxyproline and significantly attenuated expressions of collagen p-Stat3 / StatⅠ, α-SMA, TGF-β1, p-Jak2 / Jak2,and 3 in the rat liver fibrosis model and HSCs.CONCLUSION： PNS can relieve liver fibrosis by modulating Jak2/Stat3 signaling transduction pathway, which may be one of its mechanisms to suppress hepatic fibrosis.
基金the National Natural Science Foundation of China (No.50776006)Ministry of Education of China (No.107113)
文摘Experimental research was conducted to understand heat transfer characteristic of pulsating heat pipe in this paper, and the PHP is made of high quality glass capillary tube. Under different fill ratio, heat transfer rate and many other influence factors, the flow patterns were observed in the start-up, transition and stable stage. The effects of heating position on heat transfer were discussed. The experimental results indicate that no annular flow appears in top heating condition. Under different fall ratios and heat transfer rate, the flow pattern in PHP is transferred from bulk flow to semi-annular flow and annular flow, and the performance of heat transfer is improved for down heating case. The experimental results indicate that the total heat resistant of PHP is increased with fill ratio, and heat transfer rate achieves optimum at filling rate 50%. But for pulsating heat pipe with changing diameters the thermal resistance is higher than that with uniform diameters.
