Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mit...Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle. Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B （LMB）, a specific inhibitor of Crml, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. In this study, we identified five novel nuclear export signals （NESs） in pericentrin with diverse export activities. All of the five NESs could bind to Crml in a LMB-sensitive way when mediating the nuclear export of pericentrin. We also demonstrated that the region of amino acids 8-42 in pericentrin contains a tripartite nuclear localization signal （NLS） consisting of three clusters of basic amino acids. The NLS of pericentrin binds to importin β directly or via the adaptor importin α to form the import complex, which could be disrupted by RanQ69L, a dominant-negative Ran GTPase possessing high affinity for importin β. Furthermore, we found that mutation of the NESs in full-length pericentrin results in both nuclear and cytoplasmic localization, and mutation of the NLS abolishes the nuclear import of pericentrin. On the basis of these results, we suggest that the NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.展开更多
The early censorship of the English Bible was imposed during the sixteenth century. One example is William Tyndale＇s pocket-size New Testament translation, which underwem destruction including the burning of its manu...The early censorship of the English Bible was imposed during the sixteenth century. One example is William Tyndale＇s pocket-size New Testament translation, which underwem destruction including the burning of its manuscript, the published translation texts, and even the printers and possessors of these materials. This article reviews the legal history and the religious significance of the censorship, which further influences the appearance of the Authorized Version （1611 ）. Related facts are parliamentary policies concerning imports of foreign priming, book licensing and the use of the Bible. The latter part of this paper reviews evems organized by Wolsey, Tunstall and Warham, and the launch of scholarly based Bible-translation. This paper concludes by re-emphasizing the significance of these parties in sustaining the divine preservation and development of Biblical literature-readers, translators, printers, traders, clergy and legislators. They made the Phoenix rise from the heap of ashes to heaven, and inspired the work of the King James Bible.展开更多
文摘Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle. Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B （LMB）, a specific inhibitor of Crml, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. In this study, we identified five novel nuclear export signals （NESs） in pericentrin with diverse export activities. All of the five NESs could bind to Crml in a LMB-sensitive way when mediating the nuclear export of pericentrin. We also demonstrated that the region of amino acids 8-42 in pericentrin contains a tripartite nuclear localization signal （NLS） consisting of three clusters of basic amino acids. The NLS of pericentrin binds to importin β directly or via the adaptor importin α to form the import complex, which could be disrupted by RanQ69L, a dominant-negative Ran GTPase possessing high affinity for importin β. Furthermore, we found that mutation of the NESs in full-length pericentrin results in both nuclear and cytoplasmic localization, and mutation of the NLS abolishes the nuclear import of pericentrin. On the basis of these results, we suggest that the NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.
文摘The early censorship of the English Bible was imposed during the sixteenth century. One example is William Tyndale＇s pocket-size New Testament translation, which underwem destruction including the burning of its manuscript, the published translation texts, and even the printers and possessors of these materials. This article reviews the legal history and the religious significance of the censorship, which further influences the appearance of the Authorized Version （1611 ）. Related facts are parliamentary policies concerning imports of foreign priming, book licensing and the use of the Bible. The latter part of this paper reviews evems organized by Wolsey, Tunstall and Warham, and the launch of scholarly based Bible-translation. This paper concludes by re-emphasizing the significance of these parties in sustaining the divine preservation and development of Biblical literature-readers, translators, printers, traders, clergy and legislators. They made the Phoenix rise from the heap of ashes to heaven, and inspired the work of the King James Bible.
