Effects of Res on proliferation and apoptosis of human cervical carcinoma cell lines C33A,SiHa and HeLa

Objective:To explore the effects of Res on the proliferation and apoptosis of human cervical cancer cell lines C33A,SiHa and HeLa.Methods:Inhibition rates by different concentrations of Res were calculated using MTT method.Apoptosis rates and cell cycles were measured and examined by flow cytometry(FCM).Morphological configuration of apoptotic cells were observed under the fluorescence microscope.Results:The growth of cancer cells was inhibited by Res of varied concentrations in a time-and dose-dependent manner(P<0.01).The cells showed characteristic apoptosis morphologic changes under fluorescence microscope.Res exerted no effects on cell cycles.Conclusion:Res inhibits the growth of cervical cell lines C33A,SiHa and HeLa by inducing cell apoptosis in a time-and dose-dependent manner.

Laparoscopic surgery in endometrial carcinoma staging operation:analysis of 39 cases

Objective:The purpose of our study was to investigate the feasibility and short-term therapeutic effects of laparoscopic staging operation in women with endometrial carcinoma.Methods:We analyzed 86 patients with endometrial carcinoma in PLA general hospital between 2006 and 2009 retrospectively.Thirty-nine patients were performed laparoscopic modified radical hysterectomy plus systemic retroperitoneal lymphadenectomy.Forty-seven patients received traditional abdominal radical hysterectomy plus systemic retroperitoneal lymphadenectomy.We compared the operation time,blood loss,number of lymph nodes retrieved,time for restoration of gastrointestinal function,postoperative complications and morbidity,the incidence of wound infection,the length of hospital stay,and hospital charges.Results:There was no significant deviation between the two groups in age,clinical stage,and pathology.We found that there was no significant deviation between the two groups in the number of lymph nodes retrieved,postoperative complications,the rate of wound infection or hospital charge(P > 0.05).The laparoscopic group had an advantage in blood loss,time for restoration of gastrointestinal function,time for postoperative hospital stay(P < 0.05).Conclusion:Laparoscopic surgery,as a primary surgical intervention,seems to be a safe and feasible option especially in patients with early endometrial cancer.

Clinical Efficacy and Safety Analysis of Laparoscopic- assisted Vaginal Surgery and Laparoscopic-alone Surgery in Treatment of Endometrial Cancer

Objective： Assess clinical efficacy and safety analysis of laparoscopic-assisted vaginal surgery and laparoscopic-alone surgery in treatment of endometrial cancer. Methods： select 76 cases of endometrial cancer patients, divided into two groups, 40 in laparoscopic-alone group and 36 in laparoscopic-assisted group, and compare operation time, intraoperative blood loss, intraoperative complications, postoperative complications, lymph node number, anal exhaust time, indwelling catheter days and hospitalization days by group. Result： compared with laparoscopic-alone surgery, laparoscopic-assisted vaginal surgery has certain advantages in reducing intraoperative blood loss, intraoperative and postoperative complications, anal exhaust time, indwelling catheter days and hospitalization days. Discussion： laparoscopic-assisted vaginal surgery is a relatively safe, effective and good economic benefit surgical method in treatment of endometrial cancer.

阴道镜与超薄细胞检查在宫颈癌筛查中的应用研究

目的 通过液基细胞学检测、巴氏涂片、阴道镜检查,结合病理结果,探索能够提高细胞学检查阳性率的方法.方法 液基细胞学检测按说明操作,依照TBS诊断系统;常规巴氏涂片;阴道镜检查按说明操作诊断.结果 超薄细胞检查16 826例,阳性669例,阳性率占4%.经巴氏涂片1 126例,阳性2例,阳性率占0.2%.428例经阴道镜检查并活检,组织病理阳性409例,阳性率占95.5%.223例常规四点活检,组织病理阳性141例,阳性率占63.2%.结论 液基细胞学检测联合阴道镜检查,二者配合,相互结合,有利于提高癌细胞的检出率.