Down-Modulation of Notch1 Expression in Cervical Cancer Is Associated with HPV-Induced Carcinogenesis

OBJECTIVE Notch1 signaling has been implicated intumorigenesis. The purpose of this study was to investigate theputative role of the Notch1 receptor in carcinogenesis and in theprogression of the cervical cancer. Since human papillomavirus(HPV) is a causative agent in cervical carcinoma, the interactionbetween Notch1 and HPV infection was examined.METHODS Forty cervical cancer samples and 30 normalcervical tissue specimens were examined using Western blot andRT-PCR to detect Notch1 protein and mRNA levels. HPV16 DNAwas examined in all samples using PCR.RESULTS The level of Notch1 protein expression wassignificantly lower in cervical cancer tissue than in normal tissue.Levels of Notch1 mRNA were found to be substantially downregulatedin cancer tissue. Notch1 protein expression levelswere significantly higher in carcinomas without HPV DNAthan that in carcinomas with HPV infection (55.5% vs. 3.3%, P <0.05). Down-modulation of Notch1 mRNA levels in carcinomawas demonstrated to be associated with HPVE6 transcription.Moreover, levels of Notch1 expression were shown to besignificantly higher in early stage disease than in advanced stagedisease (P = 0.001).CONCLUSION Down-modulation of Notch1 expressionprobably plays an important role in the late stages of HPVinducedcervical cancer.

探讨HPV在宫颈炎、宫颈癌前病变、宫颈癌中的检测意义

目的:分析HPV在宫颈炎、宫颈癌前病变、宫颈癌中的检测意义。方法:本次研究抽取我院2015年3月-2017年4月期间收治的60例慢性宫颈炎者、60例宫颈癌前病变者以及60例宫颈癌者,将其设为A组、B组以及C组,随后对以上患者进行取样,并对其HPV予以检测。结果:通过HPV检测可知,A组60例患者中阳性例数6例,B组60例患者中阳性例数28例,C组60例患者中阳性例数38例,与此同时B组患者阳性比例高于A组患者,C组患者阳性比例高于B组,数据间经比对差异性存在,P<0.05。结论:引发宫颈癌以及宫颈癌前病变的主要因素为感染HPV,对于宫颈炎、宫颈癌前病变、宫颈癌患者而言实施HPV检测意义重大。