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鸡CAST基因5′侧翼区G-198A突变对启动子转录活性的影响 被引量:1
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作者 李春苗 黎寿丰 +3 位作者 赵振华 黄华云 王钱保 薛龙岗 《中国家禽》 北大核心 2017年第2期10-15,共6页
钙蛋白酶抑制蛋白在宰后嫩度和肌原纤维更新过程中起着关键的作用。为探讨CAST基因启动区G-198A突变位点不同基因型启动子活性,构建鸡CAST基因5′调控区CAST517序列的不同基因型双荧光素酶报告基因表达载体(pGL3-CAST517/promoter),采... 钙蛋白酶抑制蛋白在宰后嫩度和肌原纤维更新过程中起着关键的作用。为探讨CAST基因启动区G-198A突变位点不同基因型启动子活性,构建鸡CAST基因5′调控区CAST517序列的不同基因型双荧光素酶报告基因表达载体(pGL3-CAST517/promoter),采用脂质体转染法将不同基因型报告基因表达载体质粒(pGL3-CAST517G/promoter和pGL3-CAST517A/promoter)、空白对照质粒(pGL3-Basic)和海肾荧光素酶载体质粒pRL-CMV共转染至293T细胞,利用t检验法分析不同基因型的启动子活性,在线工具对CAST517序列结合位点和转录因子进行预测,分析转录水平。结果显示,CAST517A作为启动子时,萤火虫对海肾荧光素酶的强度比值要比使用CAST517G启动子效率提高113.89%,呈高效表达(P<0.01);在线预测结果显示,CAST517序列存在1个类似TATA盒、1个转录起点、3个潜在的顺式调控原件(PR、ER和GATA-1)和1个潜在的反式调控原件(TGGCA-binding)。携带CAST517A的个体具有较高的转录水平,说明该基因可能对鸡肉质性状有一定的调控作用,研究结果为CAST基因的表达和功能及优质肉鸡的选育提供了可靠依据。 展开更多
关键词 肉鸡 CAST基因 启动子 肉质性状 荧光素酶报告基因 宰后嫩度
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Effect of Delay Chilling on μ-calpain Activities and the Tenderness of Bovine M. longissimus
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作者 胡鹏 丁玉 +1 位作者 梁荣蓉 罗欣 《Agricultural Science & Technology》 CAS 2010年第5期90-92,158,共4页
μ-calpain activities and shear force values of bovine M. longissimus from Chinese Yellow crossbred bulls were analyzed,and the effect of delay chilling on μ-calpain activities and the tenderness of beef during postm... μ-calpain activities and shear force values of bovine M. longissimus from Chinese Yellow crossbred bulls were analyzed,and the effect of delay chilling on μ-calpain activities and the tenderness of beef during postmortem aging were studied. The results showed that delay chilling significantly improved μ-calpain activities (P<0.05) and enhanced the tenderness of bovine M. longissimus during earlier aging periods compared with conventional chilling. But in later aging periods,delay chilling weakened the effect on the tenderness of beef because of premature consumption of μ-calpain. The experiment results confirmed that delay chilling improved the rate of postmortem aging of beef and remarkably enhanced the tenderness of beef through the effect of delay chilling on μ-calpain activities. 展开更多
关键词 Delay chilling Shear force values Tendemess μ-calpain activities
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