本文对两个品系家免的心肌、骨骼肌、肝、肺、脑、肾、胃以及睾丸等8种组织的乳酸脱氢酶(LDH)同工酶进行了电泳比较.发现两者的 LDH 同工酶中组织分布,除骨骼肌和肝组织有较明显区别外,其余组织无显著差异.从其电泳图谱发现 LDH 同工酶...本文对两个品系家免的心肌、骨骼肌、肝、肺、脑、肾、胃以及睾丸等8种组织的乳酸脱氢酶(LDH)同工酶进行了电泳比较.发现两者的 LDH 同工酶中组织分布,除骨骼肌和肝组织有较明显区别外,其余组织无显著差异.从其电泳图谱发现 LDH 同工酶的变异体.根据 LDH—1(H 或称 B 亚基纯合体)出现亚成分,而 LDH—5(M 或称 A 亚基纯合体)未发现亚成分,确定变异体的出现是由于 H 亚基位点发生突变,出现等位基因所致.由于电泳图谱上变异体亚带数普遍少于按自由聚合规律所得的理论值,为此,对正常的 H 亚基、变异的 H~*亚基以及 M 亚基相互间亲和力的差异进行了分析和讨论.展开更多
In previous studies, N,O-carboxymethyl chitosan has been shown to decrease the incidence and intensity of abdominal adhesions. In the present study, adhesions were induced in 220 rabbits using a double uterine horn mo...In previous studies, N,O-carboxymethyl chitosan has been shown to decrease the incidence and intensity of abdominal adhesions. In the present study, adhesions were induced in 220 rabbits using a double uterine horn model. Rabbits were randomized to receive an operation only or an operation+medical chitosan intraperitoneally. Twenty-two rabbits from each group were euthanized at one of five different times(Day 3, 7, 14, 28, or 42), and adhesion formation was given gross and histopathological scores. Reductions were observed in adhesion extent(P=0.0337) and tenacity(P=0.0271) as well as inflammation(P<0.0001) on Day 3 when medical chitosan was applied. Prior to Day 14, fibrosis was less obvious in the medical chitosan group(P<0.0005). The tenacity scores were significantly lower in the medical chitosan group following Day 14(P<0.05), while the type scores were lower in the medical chitosan group following Day 28(P<0.03). Thus, medical chitosan decreased both the gross and the histopathological scores of the induced adhesions.展开更多
Objective： To explore the pathophysiological and biomechanical features of skeletal muscular injury for providing a rational basis for its treatment, prevention and rehabilitation. Methods ： In 70 adult rabbits, th...Objective： To explore the pathophysiological and biomechanical features of skeletal muscular injury for providing a rational basis for its treatment, prevention and rehabilitation. Methods ： In 70 adult rabbits, the left tibialis anterior （TA） muscle was stretched to injury, while the right TA muscle served as control. Histological, enzymohistochemical and biomechanical changes were observed on days 0, 1, 2, 3, and 7 after injury. Cytochrome oxidase （ CCO ）, acid phosphatase （ ACP ）, ATPase, succinate dehydrogenase （ SDH ）, malate dehydrogenase （ MDH ）, NADH-diaphorase （ NADHD ）, giutamatedehydrogenase （ GDH ）, α-giycerophosphate dehydrogenase （ α-GPD ） and lactate dehydrogenase （LDH） were measured. The examined biomechanical parameters included maximal contractile force, ultimate load, length, energy absorption, tangent stiffness, and rupture site. Results： Partial or complete rupture of TA muscle occurred near the muscle-tendon junction. There was an intense inflammatory reaction on day 1 and 2 after injury. Endomysium fibrosis and myotube formation were observed on day 3, and developed further on day 7. The activity of cell oxidases （ CCO, ATPase, MDH, α-GPD, SDH,NADHD and GDH） showed a significant drop from day 0 to 2, and resumed with different levels on day 3. The increment of enzymatic activities continued on day 7 and the levels of NADHD and α-GPD reached to the levels of control muscle. Maximal contractile force was 70.17 % ± 3.82% of controls immediately after injury, 54.82% ±3.09% at 1 day, 66.41% ±4.36% at 2 days, 78.39%± 4.90 % at 3 days and 93.64 % ±5.02 % at 7 days. Ultimate load was 85.78 %±7.54 % of controls at the moment of injury, 61.44 % ± 5.91% at 1 day, 49. 17 %± 4.26 % at 2 days, 64.43% ±5.02% at 3 days, and 76.71% ±6.46% at 7 days. Conclusions ： Endomysium fibrosis and scar formation at the injured site are responsible for frequent recurrence of skeletal muscle injury. Recovery of tensile load slower than that of maximal contractile force may be another cause. Whether the injured muscle returns to normal exercise is mainly determined by the teusility on which the muscle-tendon can bear rather than the maximal contractile force.展开更多
文摘本文对两个品系家免的心肌、骨骼肌、肝、肺、脑、肾、胃以及睾丸等8种组织的乳酸脱氢酶(LDH)同工酶进行了电泳比较.发现两者的 LDH 同工酶中组织分布,除骨骼肌和肝组织有较明显区别外,其余组织无显著差异.从其电泳图谱发现 LDH 同工酶的变异体.根据 LDH—1(H 或称 B 亚基纯合体)出现亚成分,而 LDH—5(M 或称 A 亚基纯合体)未发现亚成分,确定变异体的出现是由于 H 亚基位点发生突变,出现等位基因所致.由于电泳图谱上变异体亚带数普遍少于按自由聚合规律所得的理论值,为此,对正常的 H 亚基、变异的 H~*亚基以及 M 亚基相互间亲和力的差异进行了分析和讨论.
文摘In previous studies, N,O-carboxymethyl chitosan has been shown to decrease the incidence and intensity of abdominal adhesions. In the present study, adhesions were induced in 220 rabbits using a double uterine horn model. Rabbits were randomized to receive an operation only or an operation+medical chitosan intraperitoneally. Twenty-two rabbits from each group were euthanized at one of five different times(Day 3, 7, 14, 28, or 42), and adhesion formation was given gross and histopathological scores. Reductions were observed in adhesion extent(P=0.0337) and tenacity(P=0.0271) as well as inflammation(P<0.0001) on Day 3 when medical chitosan was applied. Prior to Day 14, fibrosis was less obvious in the medical chitosan group(P<0.0005). The tenacity scores were significantly lower in the medical chitosan group following Day 14(P<0.05), while the type scores were lower in the medical chitosan group following Day 28(P<0.03). Thus, medical chitosan decreased both the gross and the histopathological scores of the induced adhesions.
文摘Objective： To explore the pathophysiological and biomechanical features of skeletal muscular injury for providing a rational basis for its treatment, prevention and rehabilitation. Methods ： In 70 adult rabbits, the left tibialis anterior （TA） muscle was stretched to injury, while the right TA muscle served as control. Histological, enzymohistochemical and biomechanical changes were observed on days 0, 1, 2, 3, and 7 after injury. Cytochrome oxidase （ CCO ）, acid phosphatase （ ACP ）, ATPase, succinate dehydrogenase （ SDH ）, malate dehydrogenase （ MDH ）, NADH-diaphorase （ NADHD ）, giutamatedehydrogenase （ GDH ）, α-giycerophosphate dehydrogenase （ α-GPD ） and lactate dehydrogenase （LDH） were measured. The examined biomechanical parameters included maximal contractile force, ultimate load, length, energy absorption, tangent stiffness, and rupture site. Results： Partial or complete rupture of TA muscle occurred near the muscle-tendon junction. There was an intense inflammatory reaction on day 1 and 2 after injury. Endomysium fibrosis and myotube formation were observed on day 3, and developed further on day 7. The activity of cell oxidases （ CCO, ATPase, MDH, α-GPD, SDH,NADHD and GDH） showed a significant drop from day 0 to 2, and resumed with different levels on day 3. The increment of enzymatic activities continued on day 7 and the levels of NADHD and α-GPD reached to the levels of control muscle. Maximal contractile force was 70.17 % ± 3.82% of controls immediately after injury, 54.82% ±3.09% at 1 day, 66.41% ±4.36% at 2 days, 78.39%± 4.90 % at 3 days and 93.64 % ±5.02 % at 7 days. Ultimate load was 85.78 %±7.54 % of controls at the moment of injury, 61.44 % ± 5.91% at 1 day, 49. 17 %± 4.26 % at 2 days, 64.43% ±5.02% at 3 days, and 76.71% ±6.46% at 7 days. Conclusions ： Endomysium fibrosis and scar formation at the injured site are responsible for frequent recurrence of skeletal muscle injury. Recovery of tensile load slower than that of maximal contractile force may be another cause. Whether the injured muscle returns to normal exercise is mainly determined by the teusility on which the muscle-tendon can bear rather than the maximal contractile force.
