The interaction mechanism of collector DLZ in the flotation process of chalcopyrite and pyrite was investigated through flotation experiments,zeta potential measurements and infrared spectrum analysis.Flotation test r...The interaction mechanism of collector DLZ in the flotation process of chalcopyrite and pyrite was investigated through flotation experiments,zeta potential measurements and infrared spectrum analysis.Flotation test results indicate that DLZ is the selective collector of chalcopyrite.Especially,the recovery of chalcopyrite is higher than 90% in neutral and weak alkaline systems,while the recovery of pyrite is less than 10%.When using CaO as pH regulator,at pH=7-11,the floatability of pyrite is depressed and the recovery is less than 5%.Zeta potential analysis shows that the zeta potential of chalcopyrite decreases more obviously than that of pyrite after interaction with DLZ,confirming that collector DLZ shows selectivity to chalcopyrite and pyrite.And FTIR results reveal that the flotation selectivity of collector DLZ is due to chemical absorption onto chalcopyrite surface and only physical absorption onto pyrite surface.展开更多
Gene-centromere mapping is an essential prerequisite for understanding the composition and structure of genomes. Half-tetrad analysis is a powerful tool for mapping genes and understanding chromosomal behavior during ...Gene-centromere mapping is an essential prerequisite for understanding the composition and structure of genomes. Half-tetrad analysis is a powerful tool for mapping genes and understanding chromosomal behavior during meiosis. The Japanese scallop(Patinopecten yessoensis), a cold-tolerant species inhabiting the northwestern Pacific coast, is a commercially important marine bivalve in Asian countries. In this study, inheritance of 32 informative microsatellite loci was examined in 70-h D-shaped larvae of three induced meiogynogenetic diploid families of P. yessoensis for centromere mapping using half-tetrad analysis. The ratio of gynogenetic diploids was proven to be 100%, 100% and 96% in the three families, respectively. Inheritance analysis in the control crosses showed that 51 of the 53 genotypic ratios observed were in accordance with Mendelian expectations at the 5% level after Bonferroni correction. Seven of the 32 microsatellite loci showed the existence of null alleles in control crosses. The second division segregation frequency(y) of the microsatellite loci ranged from 0.07 to 0.85 with a mean of 0.38, suggesting the existence of positive interference after a single chiasma formation in some chromosomes in the scallop. Microsatellite-centromere distances ranged from 4 c M to 42 c M under the assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will represent a contribution towards the assembly of genetic maps in the commercially important scallop species.展开更多
基金Project(50674102) supported by the National Natural Science Foundation of China
文摘The interaction mechanism of collector DLZ in the flotation process of chalcopyrite and pyrite was investigated through flotation experiments,zeta potential measurements and infrared spectrum analysis.Flotation test results indicate that DLZ is the selective collector of chalcopyrite.Especially,the recovery of chalcopyrite is higher than 90% in neutral and weak alkaline systems,while the recovery of pyrite is less than 10%.When using CaO as pH regulator,at pH=7-11,the floatability of pyrite is depressed and the recovery is less than 5%.Zeta potential analysis shows that the zeta potential of chalcopyrite decreases more obviously than that of pyrite after interaction with DLZ,confirming that collector DLZ shows selectivity to chalcopyrite and pyrite.And FTIR results reveal that the flotation selectivity of collector DLZ is due to chemical absorption onto chalcopyrite surface and only physical absorption onto pyrite surface.
基金supported by the grants from National Marine Public Welfare Research Program (201305005)the National Natural Science Foundation of China (31072207)Doctoral Program of Ministry of Education of China (20130132110009)
文摘Gene-centromere mapping is an essential prerequisite for understanding the composition and structure of genomes. Half-tetrad analysis is a powerful tool for mapping genes and understanding chromosomal behavior during meiosis. The Japanese scallop(Patinopecten yessoensis), a cold-tolerant species inhabiting the northwestern Pacific coast, is a commercially important marine bivalve in Asian countries. In this study, inheritance of 32 informative microsatellite loci was examined in 70-h D-shaped larvae of three induced meiogynogenetic diploid families of P. yessoensis for centromere mapping using half-tetrad analysis. The ratio of gynogenetic diploids was proven to be 100%, 100% and 96% in the three families, respectively. Inheritance analysis in the control crosses showed that 51 of the 53 genotypic ratios observed were in accordance with Mendelian expectations at the 5% level after Bonferroni correction. Seven of the 32 microsatellite loci showed the existence of null alleles in control crosses. The second division segregation frequency(y) of the microsatellite loci ranged from 0.07 to 0.85 with a mean of 0.38, suggesting the existence of positive interference after a single chiasma formation in some chromosomes in the scallop. Microsatellite-centromere distances ranged from 4 c M to 42 c M under the assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will represent a contribution towards the assembly of genetic maps in the commercially important scallop species.
