Based on the kairomone from the accessory gland of female Bombyx mori elicting the parasitoid, Telenomus theophilae, to parasitize artifical beads. A cDNA expression library of enrich kairomone gene was constr...Based on the kairomone from the accessory gland of female Bombyx mori elicting the parasitoid, Telenomus theophilae, to parasitize artifical beads. A cDNA expression library of enrich kairomone gene was constructed using mRNA rich secretory portion of the accessory glands of B. mori at the 9 day old of pupa. The titer of the unamplified library was 3.29×10 4 pfu/μL with a recombinant rate of 90.05%, and titer of amplified library was 1.56×10 7 pfu/μL. The inserted cDNA fragments of the library ranged from 0.5 to 8.0 kb and concentrated around the 2.5 kb, 1.1kb, and 0.75kb regions. All of the data suggest that the library has appropriate titer and high frequency kairomone gene fragments by using special developing stage and tissue of B. mori to extract mRNA. Directional cloning and λZiplox expression were help to clone the kairomone gene with immunoscreening.展开更多
文摘Based on the kairomone from the accessory gland of female Bombyx mori elicting the parasitoid, Telenomus theophilae, to parasitize artifical beads. A cDNA expression library of enrich kairomone gene was constructed using mRNA rich secretory portion of the accessory glands of B. mori at the 9 day old of pupa. The titer of the unamplified library was 3.29×10 4 pfu/μL with a recombinant rate of 90.05%, and titer of amplified library was 1.56×10 7 pfu/μL. The inserted cDNA fragments of the library ranged from 0.5 to 8.0 kb and concentrated around the 2.5 kb, 1.1kb, and 0.75kb regions. All of the data suggest that the library has appropriate titer and high frequency kairomone gene fragments by using special developing stage and tissue of B. mori to extract mRNA. Directional cloning and λZiplox expression were help to clone the kairomone gene with immunoscreening.
