The effect of transgenic double genes, Cry1A + CpTI cotton and Cry1Ac toxinon the parasitoid, Campoketis chlorideae Uchida of cotton bollworm, Helicoverpa armigera (Huebner),was investigated in the laboratory. Helicov...The effect of transgenic double genes, Cry1A + CpTI cotton and Cry1Ac toxinon the parasitoid, Campoketis chlorideae Uchida of cotton bollworm, Helicoverpa armigera (Huebner),was investigated in the laboratory. Helicoverpa armigera larvae when in the first, second and thirdinstar could not survive if fed on transgenic cotton leaves. Consequently, C. chlorideae larvaecould not complete their development if parasitizing on such hosts. After H. armigera larvae werereared on transgenic or traditional cotton leaves for 12-48 hours, they were parasitized by C.chlorideae females. Parasitized larvae continued to feed on transgenic or traditional cotton for12-48 h. The present results showed that the body weight of larvae of the parasitoids weresignificantly reduced when parasitized hosts fed on transgenic cotton leaves compared to those fedon traditional cotton. Duration of egg and larvae stage were significantly prolonged, pupal andadult weight of C. chloridae was decreased when the host larvae fed on transgenic cotton leaveslonger than 48 h. The development duration of C. chlorideae pupae on the hosts fed on transgeniccotton leaves in each treatment was not significantly different from those of controls. Thelongevity of parasitoid females and males fed with a solution containing Cry1Ac toxin was notsignificantly different with that of the control.展开更多
Field doses of six selected insecticides were tested against the immature(pupae) and mature (adult) stages of Diadegma semiclausum (Hellen) and Oomyzus sokolowskii(Kurdjumov), parasitoids of the diamondback moth, Plut...Field doses of six selected insecticides were tested against the immature(pupae) and mature (adult) stages of Diadegma semiclausum (Hellen) and Oomyzus sokolowskii(Kurdjumov), parasitoids of the diamondback moth, Plutella xylostella (L.). Effects of contacttoxicity (direct spraying) of the six insecticides on emergence of parasitoids were found negligibleon both species except permethrin which caused 37.5% mortality. All adults of both parasitoidspecies died 24 hours after exposure to chlorfenapyr, emamectin benzoate and permethrin. Incontrast, the three insect growth regulators (IGRs), chlorfluazuron, flufenoxuron and teflubenzuron,were found harmless to both species, and adult mortality of both parasitoid species was 0—16.7%.However, parasitism by the females of both parasitoid species was severely impaired when the femaleswere offered the three IGR diluted solutions for 24 hours. Effects of oral toxicities of the IGRson longevity of both parasitoids after 12 hours exposure were found to be significantly differentbetween males and females. Compatibility of tested insecticides with D. semiclausum and O.sokolowskii and integration of compatible insecticides with these parasitoids in integrated pestmanagement programs of crucifers are discussed.展开更多
文摘The effect of transgenic double genes, Cry1A + CpTI cotton and Cry1Ac toxinon the parasitoid, Campoketis chlorideae Uchida of cotton bollworm, Helicoverpa armigera (Huebner),was investigated in the laboratory. Helicoverpa armigera larvae when in the first, second and thirdinstar could not survive if fed on transgenic cotton leaves. Consequently, C. chlorideae larvaecould not complete their development if parasitizing on such hosts. After H. armigera larvae werereared on transgenic or traditional cotton leaves for 12-48 hours, they were parasitized by C.chlorideae females. Parasitized larvae continued to feed on transgenic or traditional cotton for12-48 h. The present results showed that the body weight of larvae of the parasitoids weresignificantly reduced when parasitized hosts fed on transgenic cotton leaves compared to those fedon traditional cotton. Duration of egg and larvae stage were significantly prolonged, pupal andadult weight of C. chloridae was decreased when the host larvae fed on transgenic cotton leaveslonger than 48 h. The development duration of C. chlorideae pupae on the hosts fed on transgeniccotton leaves in each treatment was not significantly different from those of controls. Thelongevity of parasitoid females and males fed with a solution containing Cry1Ac toxin was notsignificantly different with that of the control.
文摘Field doses of six selected insecticides were tested against the immature(pupae) and mature (adult) stages of Diadegma semiclausum (Hellen) and Oomyzus sokolowskii(Kurdjumov), parasitoids of the diamondback moth, Plutella xylostella (L.). Effects of contacttoxicity (direct spraying) of the six insecticides on emergence of parasitoids were found negligibleon both species except permethrin which caused 37.5% mortality. All adults of both parasitoidspecies died 24 hours after exposure to chlorfenapyr, emamectin benzoate and permethrin. Incontrast, the three insect growth regulators (IGRs), chlorfluazuron, flufenoxuron and teflubenzuron,were found harmless to both species, and adult mortality of both parasitoid species was 0—16.7%.However, parasitism by the females of both parasitoid species was severely impaired when the femaleswere offered the three IGR diluted solutions for 24 hours. Effects of oral toxicities of the IGRson longevity of both parasitoids after 12 hours exposure were found to be significantly differentbetween males and females. Compatibility of tested insecticides with D. semiclausum and O.sokolowskii and integration of compatible insecticides with these parasitoids in integrated pestmanagement programs of crucifers are discussed.
