AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and ...AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and trophozoites isolated from a patient with amebiasis were cultivated in the medium, incubated at 37℃ for a period of 4 days and 40×10~4/ml amebic cysts were then transferred to a fresh medium.At the second stage,0.05,0.1 and 0.2 mM of selected metal ions were added to the medium,and the effects of these ions on parasitic reproduction compared with the control group were observed. RESULTS:It was determined that the number of living parasites in all the groups containing metal ions decreased significantly starting from 30 minutes (P<0.01).CuCl_2 showed the highest lethal effect on E.histolytica cysts,whereas the lowest lethal effect was observed with CoCl_2.It was also seen that the number of living cells was decreased as the ion concentration and exposure time were increased,and that there were no living parasites in the medium at the end of 24 h (P<0.01). CONCLUSION:It may be stated that the effect of ever- increasing contamination of the environment with metal waste materials on parasites should be investigated further.展开更多
In order to apply the nematode-trapping fungi biocontrol of animal parasitic nematode, prove that mutagenesis strain of the Arthrobotrys Oligospora N spores can be passed to experimental animal digestive tract. Ion be...In order to apply the nematode-trapping fungi biocontrol of animal parasitic nematode, prove that mutagenesis strain of the Arthrobotrys Oligospora N spores can be passed to experimental animal digestive tract. Ion beam injected into the spores of Arthrobotrys oligospora of nematode-trapping.Experimental animals are hurled Arthrobotrys Oligospora N spores, collect the feces of experimental animals, test fungal germination, growth, reproduction and predation livestock parasitic nematode larvae in laboratory. Mutant strains of Arthrobotrys Oligospora N can be through the digestive tract in experimental animals. Such biotechnology could be explored for improving the effectiveness of the use of funsal infections to control livestock parasitic nematodes.展开更多
文摘AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and trophozoites isolated from a patient with amebiasis were cultivated in the medium, incubated at 37℃ for a period of 4 days and 40×10~4/ml amebic cysts were then transferred to a fresh medium.At the second stage,0.05,0.1 and 0.2 mM of selected metal ions were added to the medium,and the effects of these ions on parasitic reproduction compared with the control group were observed. RESULTS:It was determined that the number of living parasites in all the groups containing metal ions decreased significantly starting from 30 minutes (P<0.01).CuCl_2 showed the highest lethal effect on E.histolytica cysts,whereas the lowest lethal effect was observed with CoCl_2.It was also seen that the number of living cells was decreased as the ion concentration and exposure time were increased,and that there were no living parasites in the medium at the end of 24 h (P<0.01). CONCLUSION:It may be stated that the effect of ever- increasing contamination of the environment with metal waste materials on parasites should be investigated further.
基金Acknowledgements This work was funded by the the National Natural Science Foundation of China （grant number30260081）.Inner Mongolia Natural Science Foundation（grant number 2009MS0411 ）.
文摘In order to apply the nematode-trapping fungi biocontrol of animal parasitic nematode, prove that mutagenesis strain of the Arthrobotrys Oligospora N spores can be passed to experimental animal digestive tract. Ion beam injected into the spores of Arthrobotrys oligospora of nematode-trapping.Experimental animals are hurled Arthrobotrys Oligospora N spores, collect the feces of experimental animals, test fungal germination, growth, reproduction and predation livestock parasitic nematode larvae in laboratory. Mutant strains of Arthrobotrys Oligospora N can be through the digestive tract in experimental animals. Such biotechnology could be explored for improving the effectiveness of the use of funsal infections to control livestock parasitic nematodes.
