AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and ...AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and trophozoites isolated from a patient with amebiasis were cultivated in the medium, incubated at 37℃ for a period of 4 days and 40×10~4/ml amebic cysts were then transferred to a fresh medium.At the second stage,0.05,0.1 and 0.2 mM of selected metal ions were added to the medium,and the effects of these ions on parasitic reproduction compared with the control group were observed. RESULTS:It was determined that the number of living parasites in all the groups containing metal ions decreased significantly starting from 30 minutes (P<0.01).CuCl_2 showed the highest lethal effect on E.histolytica cysts,whereas the lowest lethal effect was observed with CoCl_2.It was also seen that the number of living cells was decreased as the ion concentration and exposure time were increased,and that there were no living parasites in the medium at the end of 24 h (P<0.01). CONCLUSION:It may be stated that the effect of ever- increasing contamination of the environment with metal waste materials on parasites should be investigated further.展开更多
This study investagated the data of the prevalence of intestinal parasites of human from September 2007 to July 2008 using faecal samples which were picked from refuse dump sites. A total of 2,050 stool samples picked...This study investagated the data of the prevalence of intestinal parasites of human from September 2007 to July 2008 using faecal samples which were picked from refuse dump sites. A total of 2,050 stool samples picked and examined from five selected popular refuse dump sites in Ado-Ekiti. And Entamoeba histolytica, Giardia lamblia and Balantidium coli were mostly identified. The analysis of the infection shows that E. histolytica has a percentage prevalence of 65.5%, followed by G. lamblia with 42.5% and B. coli having 1.9%. Multiple infections of these parasites were also prevalent. There was a positive correlation （r=0.995, P〈0.05） between percentage prevalence of infection and home surroundings, waste, sewage disposal facilities and habits. Also, there was a significant difference （t=1 1.06, P〈0.05） in the prevalence of infections between location A and B. Probable ways of eliminating/controlling the disease are also highlighted.展开更多
文摘AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and trophozoites isolated from a patient with amebiasis were cultivated in the medium, incubated at 37℃ for a period of 4 days and 40×10~4/ml amebic cysts were then transferred to a fresh medium.At the second stage,0.05,0.1 and 0.2 mM of selected metal ions were added to the medium,and the effects of these ions on parasitic reproduction compared with the control group were observed. RESULTS:It was determined that the number of living parasites in all the groups containing metal ions decreased significantly starting from 30 minutes (P<0.01).CuCl_2 showed the highest lethal effect on E.histolytica cysts,whereas the lowest lethal effect was observed with CoCl_2.It was also seen that the number of living cells was decreased as the ion concentration and exposure time were increased,and that there were no living parasites in the medium at the end of 24 h (P<0.01). CONCLUSION:It may be stated that the effect of ever- increasing contamination of the environment with metal waste materials on parasites should be investigated further.
文摘This study investagated the data of the prevalence of intestinal parasites of human from September 2007 to July 2008 using faecal samples which were picked from refuse dump sites. A total of 2,050 stool samples picked and examined from five selected popular refuse dump sites in Ado-Ekiti. And Entamoeba histolytica, Giardia lamblia and Balantidium coli were mostly identified. The analysis of the infection shows that E. histolytica has a percentage prevalence of 65.5%, followed by G. lamblia with 42.5% and B. coli having 1.9%. Multiple infections of these parasites were also prevalent. There was a positive correlation （r=0.995, P〈0.05） between percentage prevalence of infection and home surroundings, waste, sewage disposal facilities and habits. Also, there was a significant difference （t=1 1.06, P〈0.05） in the prevalence of infections between location A and B. Probable ways of eliminating/controlling the disease are also highlighted.
