Haustorium formation is the characteristic feature of all parasitic plants and a vital process for successful parasitism.Previous investigations on haustorium initiation and development are constricted to induced proc...Haustorium formation is the characteristic feature of all parasitic plants and a vital process for successful parasitism.Previous investigations on haustorium initiation and development are constricted to induced processes by host-derived signals or synthetic analogs.Spontaneous haustorium formation in the absence of host signals,a process representing an early stage in the evolution of parasitic plants,remains largely unexplored.Lack of fast and frequent formation of spontaneous haustoria greatly hinders full understanding of haustorium formation in root hemiparasites.In this study,seedlings of Pedicularis kansuensis Maxim.,a facultative root hemiparasitic species in Orobanchaceae observed to produce many spontaneous haustoria,were grown in autoclaved water agar in the absence of any known haustoriuminducing stimulants.We aimed to test the temporal and developmental pattern of spontaneous haustorium formation.Also,effects of sucrose supply and root contact on spontaneous haustorium formation were tested.Spontaneous haustoria were observed starting from six days after germination,much earlier than previously reported root hemiparasites.A majority of the spontaneous haustoria formed on lateral roots.Percentage of seedlings with spontaneous haustoria was 28.8% when grown on water agar plates,with a mean of four haustoria per seedling two weeks after germination.Haustorium formation by seedlings grown in water agar amended with 2% sucrose was more than twice of those without sucrose amendment.Singly grown seedlings were able to develop spontaneous haustoria at similar levels as those grown with another conspecific seedling.In view of the fast and abundant formation of spontaneous haustoria,P.kansuensis may be developed as an excellent experimental system in future investigations for unraveling endogenous regulation of haustorium initiation and development in root hemiparasitic plants.展开更多
Tea is a perennial and evergreen plant. Cultivated tea trees provide a habitat for insect pests and their natural enemies. In Japan, granuloviruses (GVs) have successfully controlled two of the most important pests of...Tea is a perennial and evergreen plant. Cultivated tea trees provide a habitat for insect pests and their natural enemies. In Japan, granuloviruses (GVs) have successfully controlled two of the most important pests of tea, Adoxophyes honmai and Homona magnanima (Tortricidae: Lepidoptera). The GVs are produced in vivo and a single application sustains pesticidal efficacy throughout a year, which encompasses 4 to 5 discrete generations of both species. A. honmai and H. magnanima also have various natural enemies, especially hymenopteran parasitoids. Such resident natural enemies also play a role in reducing the pest density in virus-controlled fields, but the effect of virus infection on parasitoids sharing the same host larva has not been well studied. Survival of one of the major parasitoids of A. honmai, Ascogaster reticulata (Braconidae: Hymenoptera), is reduced by virus infection of the host. Viruses, including GV and entomopoxvirus (EPV), and certain koinobiont endoparasitoids, including A. reticulata, are both known to regulate host endocrinology. However, the GV and EPV have distinct host regulation mechanisms, and consequently have different impacts on the survival of A. retuculata, when A. reticulata parasitizes a host that is infected with either GV or EPV. These additional effects on host regulation displayed by both viruses and parasitoids affect the outcome of virus-parasitoid interactions.展开更多
Potato is the main material of nutrition comes after the wheat and, is subjected to attacks of different plant parasitic nematodes which cause serious damages in our country. Especially, root-knot nematodes cause seri...Potato is the main material of nutrition comes after the wheat and, is subjected to attacks of different plant parasitic nematodes which cause serious damages in our country. Especially, root-knot nematodes cause serious crop losses on them. Due to usage oflnfested seeds, parasitic nematodes are increasing from year to year. In our country, so far, many infested hosts of Meloidogyne incognita （Kofoid ＆White, 1919） Chitwood, 1949 were detected but there is no report on investigation on potato. During this study, adult females of root-knot nematodes were obtained via extracting gals of 133 tuber samples, collected potatoes fields in the Marmara region especially Balikesir, Bilecik, Bursa, Canakkale, Edirne, Istanbul, Kirklareli, Kocaeli, Sakarya and Tekirdag provinces during 2007-2008. During identification the species, second stage larvae were taken into consideration as well as genital preparations of adult females. For the identification classical methods were used together with molecular methods. As result, M. incognita was detected, only species detected among the Meloidogyne genus upon the 10 provinces investigated in Edirne province, Bosna village. This is the first report of the infestation of this species potato and also in Marmara Region.展开更多
In order to apply the nematode-trapping fungi biocontrol of animal parasitic nematode, prove that mutagenesis strain of the Arthrobotrys Oligospora N spores can be passed to experimental animal digestive tract. Ion be...In order to apply the nematode-trapping fungi biocontrol of animal parasitic nematode, prove that mutagenesis strain of the Arthrobotrys Oligospora N spores can be passed to experimental animal digestive tract. Ion beam injected into the spores of Arthrobotrys oligospora of nematode-trapping.Experimental animals are hurled Arthrobotrys Oligospora N spores, collect the feces of experimental animals, test fungal germination, growth, reproduction and predation livestock parasitic nematode larvae in laboratory. Mutant strains of Arthrobotrys Oligospora N can be through the digestive tract in experimental animals. Such biotechnology could be explored for improving the effectiveness of the use of funsal infections to control livestock parasitic nematodes.展开更多
Sericea lespedeza （Lespedeza cuneata （Dum. Cours.） G. Don.） has historically been called ＂poor man＇s alfalfa＂, because of its ability to grow on marginal soil with the minimal inputs of lime or fertilizer. With...Sericea lespedeza （Lespedeza cuneata （Dum. Cours.） G. Don.） has historically been called ＂poor man＇s alfalfa＂, because of its ability to grow on marginal soil with the minimal inputs of lime or fertilizer. With recent research detailing the potential health benefits of this forage to animals, it may be time for a new nickname. Although sericea lespedeza has been used for soil conservation and as an inexpensive （low-input） source of pasture or hay crop for nearly 100 years, research over the last 10-15 years has demonstrated the excellent bioactivity of this plant against infection with gastrointestinal nematodes （Haemonchus contortus） and protozoan parasites （Eimeria spp.） in livestock. This bioactivity, which has been attributed to a unique type of condensed tannins （CT）, has been confirmed in both fresh （grazed） and dried （hay, meal, pellets） forms of sericea lespedeza in a number of studies with sheep, goats, and cattle. There is on-going research with this forage to determine the effect of ensiling on its bioactive properties and to validate its anti-parasitic effects on-farm. Future research will focus on determining the mode of action of sericea lespedeza tannins against internal parasites and the interplay between the nutritional value and the health benefits of this plant.展开更多
Agricultural research made in recent years has found that many bacterial organisms act like biological control agents with minimal impact on the environment. These microorganisms and their toxic metabolites should be ...Agricultural research made in recent years has found that many bacterial organisms act like biological control agents with minimal impact on the environment. These microorganisms and their toxic metabolites should be included in those which are acceptable to qualify a product as organic or integrated and enjoy all the benefits that entails. Hence, interest in the use of microorganisms as biological agents to protect crop plants against plant parasitic nematodes has been increasing. This study investigated the ability of.Pseudomonas oryzihabitans symbiotically associated with the entomopathogenic nematode Steinernema abbasi as a bioagent against plant parasitic nematodes which infected tomato crop. The bacterium is particularly effective against root-knot nematode in vitro affecting the behavior and mobility of root-knot nematode juveniles. Also, studies in planta demonstrated the efficacy of P. oryzihabitans by preventing tomato root system by invasion of juveniles of Meloidogynejavaniea when bacterial cells were applied to the root system before nematodes. This efficacy is dependent on bacterial cell concentration used and the time of the nematode exposure. However, a better nematode control might be achieved with multiple applications of the biocontrol agent. Furthermore, the results of this study provide evidence that the bacterium P. oryzihabitans produces metabolites, which have nematostatic effects.展开更多
Investigations on the biodiversity of entomophagous fauna of Spilarctia obiqua revealed the occurrence of twelve different natural enemies, out of which five Hymenoptera, four Dipterous parasitoids and three predators...Investigations on the biodiversity of entomophagous fauna of Spilarctia obiqua revealed the occurrence of twelve different natural enemies, out of which five Hymenoptera, four Dipterous parasitoids and three predators viz., two Hemiptera and one Neuroptera were found attacking different stages of the host. Biological notes on four parasitoids viz. Cotesia obliqua, Goniophthalmus halli, Meteorus sp. and Cotesia （glomeratus group） were also made. Estimation of field parasitization of the three species of entomophagous insect viz. C. obliqua, G. halli, and Meteorus sp. revealed that the C. obliqua was the most dominant parasitoid and maximum 21.6 per cent parasitization was observed during the month of October followed by 16% of by Meteorus and 14% by G. halli during February. Conservation of the biodiversity of these entomophagous fauna is advocated for BIPM programme.展开更多
基金financially supported by the Natural Science Foundation of China(31370512,U1303201,and 31400440)Natural Science Foundation of Yunnan Province(2016FB059)+1 种基金funding for Airong Li from The Youth Innovation Promotion Association of Chinese Academy of Sciencesthe Young Academic and Technical Leader Raising Foundation of Yunnan Province(2014HB047)
文摘Haustorium formation is the characteristic feature of all parasitic plants and a vital process for successful parasitism.Previous investigations on haustorium initiation and development are constricted to induced processes by host-derived signals or synthetic analogs.Spontaneous haustorium formation in the absence of host signals,a process representing an early stage in the evolution of parasitic plants,remains largely unexplored.Lack of fast and frequent formation of spontaneous haustoria greatly hinders full understanding of haustorium formation in root hemiparasites.In this study,seedlings of Pedicularis kansuensis Maxim.,a facultative root hemiparasitic species in Orobanchaceae observed to produce many spontaneous haustoria,were grown in autoclaved water agar in the absence of any known haustoriuminducing stimulants.We aimed to test the temporal and developmental pattern of spontaneous haustorium formation.Also,effects of sucrose supply and root contact on spontaneous haustorium formation were tested.Spontaneous haustoria were observed starting from six days after germination,much earlier than previously reported root hemiparasites.A majority of the spontaneous haustoria formed on lateral roots.Percentage of seedlings with spontaneous haustoria was 28.8% when grown on water agar plates,with a mean of four haustoria per seedling two weeks after germination.Haustorium formation by seedlings grown in water agar amended with 2% sucrose was more than twice of those without sucrose amendment.Singly grown seedlings were able to develop spontaneous haustoria at similar levels as those grown with another conspecific seedling.In view of the fast and abundant formation of spontaneous haustoria,P.kansuensis may be developed as an excellent experimental system in future investigations for unraveling endogenous regulation of haustorium initiation and development in root hemiparasitic plants.
基金This work was partially supported by Grant-in-Aid for Scientific Research (B) (18380038)
文摘Tea is a perennial and evergreen plant. Cultivated tea trees provide a habitat for insect pests and their natural enemies. In Japan, granuloviruses (GVs) have successfully controlled two of the most important pests of tea, Adoxophyes honmai and Homona magnanima (Tortricidae: Lepidoptera). The GVs are produced in vivo and a single application sustains pesticidal efficacy throughout a year, which encompasses 4 to 5 discrete generations of both species. A. honmai and H. magnanima also have various natural enemies, especially hymenopteran parasitoids. Such resident natural enemies also play a role in reducing the pest density in virus-controlled fields, but the effect of virus infection on parasitoids sharing the same host larva has not been well studied. Survival of one of the major parasitoids of A. honmai, Ascogaster reticulata (Braconidae: Hymenoptera), is reduced by virus infection of the host. Viruses, including GV and entomopoxvirus (EPV), and certain koinobiont endoparasitoids, including A. reticulata, are both known to regulate host endocrinology. However, the GV and EPV have distinct host regulation mechanisms, and consequently have different impacts on the survival of A. retuculata, when A. reticulata parasitizes a host that is infected with either GV or EPV. These additional effects on host regulation displayed by both viruses and parasitoids affect the outcome of virus-parasitoid interactions.
文摘Potato is the main material of nutrition comes after the wheat and, is subjected to attacks of different plant parasitic nematodes which cause serious damages in our country. Especially, root-knot nematodes cause serious crop losses on them. Due to usage oflnfested seeds, parasitic nematodes are increasing from year to year. In our country, so far, many infested hosts of Meloidogyne incognita （Kofoid ＆White, 1919） Chitwood, 1949 were detected but there is no report on investigation on potato. During this study, adult females of root-knot nematodes were obtained via extracting gals of 133 tuber samples, collected potatoes fields in the Marmara region especially Balikesir, Bilecik, Bursa, Canakkale, Edirne, Istanbul, Kirklareli, Kocaeli, Sakarya and Tekirdag provinces during 2007-2008. During identification the species, second stage larvae were taken into consideration as well as genital preparations of adult females. For the identification classical methods were used together with molecular methods. As result, M. incognita was detected, only species detected among the Meloidogyne genus upon the 10 provinces investigated in Edirne province, Bosna village. This is the first report of the infestation of this species potato and also in Marmara Region.
基金Acknowledgements This work was funded by the the National Natural Science Foundation of China （grant number30260081）.Inner Mongolia Natural Science Foundation（grant number 2009MS0411 ）.
文摘In order to apply the nematode-trapping fungi biocontrol of animal parasitic nematode, prove that mutagenesis strain of the Arthrobotrys Oligospora N spores can be passed to experimental animal digestive tract. Ion beam injected into the spores of Arthrobotrys oligospora of nematode-trapping.Experimental animals are hurled Arthrobotrys Oligospora N spores, collect the feces of experimental animals, test fungal germination, growth, reproduction and predation livestock parasitic nematode larvae in laboratory. Mutant strains of Arthrobotrys Oligospora N can be through the digestive tract in experimental animals. Such biotechnology could be explored for improving the effectiveness of the use of funsal infections to control livestock parasitic nematodes.
文摘Sericea lespedeza （Lespedeza cuneata （Dum. Cours.） G. Don.） has historically been called ＂poor man＇s alfalfa＂, because of its ability to grow on marginal soil with the minimal inputs of lime or fertilizer. With recent research detailing the potential health benefits of this forage to animals, it may be time for a new nickname. Although sericea lespedeza has been used for soil conservation and as an inexpensive （low-input） source of pasture or hay crop for nearly 100 years, research over the last 10-15 years has demonstrated the excellent bioactivity of this plant against infection with gastrointestinal nematodes （Haemonchus contortus） and protozoan parasites （Eimeria spp.） in livestock. This bioactivity, which has been attributed to a unique type of condensed tannins （CT）, has been confirmed in both fresh （grazed） and dried （hay, meal, pellets） forms of sericea lespedeza in a number of studies with sheep, goats, and cattle. There is on-going research with this forage to determine the effect of ensiling on its bioactive properties and to validate its anti-parasitic effects on-farm. Future research will focus on determining the mode of action of sericea lespedeza tannins against internal parasites and the interplay between the nutritional value and the health benefits of this plant.
文摘Agricultural research made in recent years has found that many bacterial organisms act like biological control agents with minimal impact on the environment. These microorganisms and their toxic metabolites should be included in those which are acceptable to qualify a product as organic or integrated and enjoy all the benefits that entails. Hence, interest in the use of microorganisms as biological agents to protect crop plants against plant parasitic nematodes has been increasing. This study investigated the ability of.Pseudomonas oryzihabitans symbiotically associated with the entomopathogenic nematode Steinernema abbasi as a bioagent against plant parasitic nematodes which infected tomato crop. The bacterium is particularly effective against root-knot nematode in vitro affecting the behavior and mobility of root-knot nematode juveniles. Also, studies in planta demonstrated the efficacy of P. oryzihabitans by preventing tomato root system by invasion of juveniles of Meloidogynejavaniea when bacterial cells were applied to the root system before nematodes. This efficacy is dependent on bacterial cell concentration used and the time of the nematode exposure. However, a better nematode control might be achieved with multiple applications of the biocontrol agent. Furthermore, the results of this study provide evidence that the bacterium P. oryzihabitans produces metabolites, which have nematostatic effects.
文摘Investigations on the biodiversity of entomophagous fauna of Spilarctia obiqua revealed the occurrence of twelve different natural enemies, out of which five Hymenoptera, four Dipterous parasitoids and three predators viz., two Hemiptera and one Neuroptera were found attacking different stages of the host. Biological notes on four parasitoids viz. Cotesia obliqua, Goniophthalmus halli, Meteorus sp. and Cotesia （glomeratus group） were also made. Estimation of field parasitization of the three species of entomophagous insect viz. C. obliqua, G. halli, and Meteorus sp. revealed that the C. obliqua was the most dominant parasitoid and maximum 21.6 per cent parasitization was observed during the month of October followed by 16% of by Meteorus and 14% by G. halli during February. Conservation of the biodiversity of these entomophagous fauna is advocated for BIPM programme.
