猪链球菌2型(Streptococcus suis serotype2,SS2)是一种世界范围内的引起猪链球菌病的重要的人兽共患病原菌,人感染SS2后多呈现败血症型和脑膜炎型。2005年猪链球菌在我国四川省的感染暴发和近年来不断出现的零散病例给我国的公共卫生...猪链球菌2型(Streptococcus suis serotype2,SS2)是一种世界范围内的引起猪链球菌病的重要的人兽共患病原菌,人感染SS2后多呈现败血症型和脑膜炎型。2005年猪链球菌在我国四川省的感染暴发和近年来不断出现的零散病例给我国的公共卫生和食品安全带来了威胁,但迄今为止,对SS2致病机制研究尚不够深入。SS2的致病过程和宿主细胞相互作用密切相关,SS2-体外宿主细胞相互作用模型的建立对于揭示SS2致病分子机理具有重要的意义。目前的研究报道主要是从猪链球菌黏附与侵袭上皮细胞或内皮细胞、猪链球菌抗吞噬细胞的吞噬和猪链球菌激活免疫细胞炎症反应方面研究猪链球菌与宿主细胞的相互作用,但与其他常致病性链球菌如化脓性链球菌和肺炎链球菌相比,有些SS2-体外宿主细胞相互作用模型还缺乏统一的标准,由于感染模型的实验参数不尽相同得到结果也有所不同。本文对近年来猪链球菌2型与宿主细胞体外相互作用及其致病机理研究进展作一综述。展开更多
Glyceraldehyde-3-phosphate dehydro- genase (GAPDH) is a multifunctional protein well defined in eukaryotes, especially in mammalian and Saccharomyces cerevisiae. Using the method of suppression subtractive hybridizati...Glyceraldehyde-3-phosphate dehydro- genase (GAPDH) is a multifunctional protein well defined in eukaryotes, especially in mammalian and Saccharomyces cerevisiae. Using the method of suppression subtractive hybridization (SSH), we identified a Phytophthora sojae cDNA coding GAPDH, which was up-regulated during the early stage of soybean infection. The termed PsGapdh gene pos- sessed three copies in the P. sojae genome. Its amino acid sequence harbored overall conserved domain of GADPH, homologous closest to GapC1 of Achlya bisexualis (oomycete) and adjoined to GapC2s of Odontella sinensis and Phaeodactylum tricornutum (diatom), on the C-Ⅱ branch of subfamily GapC in phylogeny tree of GAPDH. The transcrip- tional level of PsGapdh was up-regulated throughout early infection. Heterogenous expression of PsGapdh in the yeast tdh1-deleted mutant could rescue growth arrest under continuous exposure to H2O2. These results indicated active roles of PsGapdh in patho- gen-host interaction and anti-oxidation.展开更多
文摘猪链球菌2型(Streptococcus suis serotype2,SS2)是一种世界范围内的引起猪链球菌病的重要的人兽共患病原菌,人感染SS2后多呈现败血症型和脑膜炎型。2005年猪链球菌在我国四川省的感染暴发和近年来不断出现的零散病例给我国的公共卫生和食品安全带来了威胁,但迄今为止,对SS2致病机制研究尚不够深入。SS2的致病过程和宿主细胞相互作用密切相关,SS2-体外宿主细胞相互作用模型的建立对于揭示SS2致病分子机理具有重要的意义。目前的研究报道主要是从猪链球菌黏附与侵袭上皮细胞或内皮细胞、猪链球菌抗吞噬细胞的吞噬和猪链球菌激活免疫细胞炎症反应方面研究猪链球菌与宿主细胞的相互作用,但与其他常致病性链球菌如化脓性链球菌和肺炎链球菌相比,有些SS2-体外宿主细胞相互作用模型还缺乏统一的标准,由于感染模型的实验参数不尽相同得到结果也有所不同。本文对近年来猪链球菌2型与宿主细胞体外相互作用及其致病机理研究进展作一综述。
文摘Glyceraldehyde-3-phosphate dehydro- genase (GAPDH) is a multifunctional protein well defined in eukaryotes, especially in mammalian and Saccharomyces cerevisiae. Using the method of suppression subtractive hybridization (SSH), we identified a Phytophthora sojae cDNA coding GAPDH, which was up-regulated during the early stage of soybean infection. The termed PsGapdh gene pos- sessed three copies in the P. sojae genome. Its amino acid sequence harbored overall conserved domain of GADPH, homologous closest to GapC1 of Achlya bisexualis (oomycete) and adjoined to GapC2s of Odontella sinensis and Phaeodactylum tricornutum (diatom), on the C-Ⅱ branch of subfamily GapC in phylogeny tree of GAPDH. The transcrip- tional level of PsGapdh was up-regulated throughout early infection. Heterogenous expression of PsGapdh in the yeast tdh1-deleted mutant could rescue growth arrest under continuous exposure to H2O2. These results indicated active roles of PsGapdh in patho- gen-host interaction and anti-oxidation.
