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菜豆富含脯氨酸蛋白质基因在生物和非生物胁迫下的表达 被引量:34
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作者 柴团耀 张玉秀 《Acta Botanica Sinica》 CSCD 1999年第1期111-113,共3页
The PvSR1(Phaseolus vulgaris stress-related protein) gene encoding an 11 kD proline-rich protein (PRP) was constitutively expansed in the primary leaf tissue of beau (Phaeolus vulgaris L. cv. Saxa). The coarse studies... The PvSR1(Phaseolus vulgaris stress-related protein) gene encoding an 11 kD proline-rich protein (PRP) was constitutively expansed in the primary leaf tissue of beau (Phaeolus vulgaris L. cv. Saxa). The coarse studies of mRNA accumulation of the PvSR1 has been studied in this plant against virus infection and various abiotic sacsses. Northern blot analysis revealed that the gene expression of PvSR1 was greatly enhanced in the leaf tissue by AIMV (alfalfa mosaic vims) infection, wounding, heat shock, UV, drought and salt stress. The PvSR1 transcripts could also be increased by the exogenous inducing fartors such as salicylic acid and H2O2. The Precise biological roles of PvSR1 is unknown, but the expression of PvSR1 genes in various form of stress suggest that PvSR1 may play an important role during the strees, in maintaining cellular integrity by forming strong linkages with the cell wall. 展开更多
关键词 菜豆 富含脯氨酸的蛋白质 生物胁迫 非生物胁迫
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miR-144在前列腺癌细胞中的表达对肿瘤增殖和迁移的影响 被引量:1
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作者 王宁宁 王超 杨小淮 《中国现代医药杂志》 2020年第1期1-4,共4页
目的探讨miR-144在前列腺癌细胞中的表达对肿瘤增殖和迁移的影响。方法采用实时定量聚合酶链(RT-qPCR)检测前列腺癌细胞株LNCaP中miR-144的表达。细胞计数试剂盒(CCK-8)检测miR-144对LNCaP细胞增殖的影响。Transwell实验检测miR-144对LN... 目的探讨miR-144在前列腺癌细胞中的表达对肿瘤增殖和迁移的影响。方法采用实时定量聚合酶链(RT-qPCR)检测前列腺癌细胞株LNCaP中miR-144的表达。细胞计数试剂盒(CCK-8)检测miR-144对LNCaP细胞增殖的影响。Transwell实验检测miR-144对LNCaP细胞迁移的影响。Western blot实验分析miR-144与富含脯氨酸的蛋白质11(PRR11)之间的关系。结果转染过 miR-144的LNCaP细胞中miR-144表达量显著高于转染miR-NC的细胞。CCK-8实验显示miR-144在体外明显抑制前列腺癌细胞株的增殖能力。Transwell实验结果表明过表达miR-144之后的LNCaP细胞体外迁移能力与转染miR-NC细胞相比显著降低。Western blot结果显示转染后LNCaP细胞中PRR11的表达量显著下降。结论 miR-144通过下调PRR11抑制前列腺癌细胞株LNCaP的增殖、迁移。 展开更多
关键词 前列腺癌 miR-144 富含脯氨酸的蛋白质11
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