A microtiter plate assay (MPA) for soluble reactive phosphorus (SRP) was applied to samples in overlying water and pore water as well as in three forms of environmental soil test phosphorus (P) types: water soluble ph...A microtiter plate assay (MPA) for soluble reactive phosphorus (SRP) was applied to samples in overlying water and pore water as well as in three forms of environmental soil test phosphorus (P) types: water soluble phosphorus (WSP),diluted calcium chloride extractable phosphorus (PCaCl2), and Olsen-P in the sediments of Taihu Lake, China, where potential P release in response to pH was analyzed. MPA for rapid P analysis was shown to be promising when applied on samples of natural water and sediment extracts. Concentrations of WSP and PCaCl2 in the sediments were much lower than those of Olsen-P. Olsen-P levels in the littoral sediments along the north coast of Meiliang Bay in Taihu Lake (80to 140 mg kg-1) were much higher than those in the mouth of the bay (less than 50 mg kg-1). The risk of P release in the mouth area of Meiliang Bay was lower than that in the north littoral zone with a risk of sediment P release induced by pH increases.展开更多
Aquatic macrophytic growth and its distribution in eutrophic lakes are described in relation to its sediment type and irradiance. Hence, it is necessary to characterize the response of macrophytic growth to lake sedim...Aquatic macrophytic growth and its distribution in eutrophic lakes are described in relation to its sediment type and irradiance. Hence, it is necessary to characterize the response of macrophytic growth to lake sediment and its re-suspension. We conducted two independent experiments to measure the effects of sediment and its re-suspension on the growth of Vallisneria asiatica Miki. Based on our study, we conclude that the amount of total biomass of V. asiatica is related, in important ways, to the type of sediment and its re-suspension. According to our comparison, plants and biomass cultured on lake sediments are 2.1 times and 1.5 times greater than that on brown clay from nearby places beside Lake Taihu. The number of plants and the amount of biomass in a turbid water column are smaller than those in a clear water column. The results indicate that the nutrient-rich lake sediments have promoted the growth of V. asiatica, while the re-suspension of the sediment leads to a high turbid level and low light penetration which has a negative impact on the growth of V. asiatica.展开更多
Lake Gehu is a severely eutrophic lake in southeast China. A series of restoration measures have been implemented since 2009 in northern Lake Gehu. This study compared aquatic plants, water quality, sediment, and phyt...Lake Gehu is a severely eutrophic lake in southeast China. A series of restoration measures have been implemented since 2009 in northern Lake Gehu. This study compared aquatic plants, water quality, sediment, and phytoplankton between restoration and control areas to investigate the effect of restoration measures. The results demonstrated that aquatic macrophyte coverage increased from 0% to 10.6%; mean TP, TN, and CODm concentrations decreased by 50.0%, 42.4%, and 40.8%, respectively, compared with those before the measures were carried out; the mean Secchi depth (SD) increased to 42.5 cm, which is 1.4 times higher than that before restoration; the mean euphotic depth (Zeu) in the summer increased from 91 to 130 cm; the mean chl a concentration decreased from 34.8 to 20.2 μg/L, compared with that before restoration; the Shannon-Wiener index of phytoplankton increased by 28.7%. The mean TP and TN concentrations in sediments decreased by 63.8% and 52.4%, respectively, compared with that before dredging. These results indicate that the restoration in northern Lake Gehu was effective. To complete the transformation from an algae- to a macrophyte-stable state within the region, further measures must be adopted. This restoration of a eutrophic lake can serve as a reference for similar eutrophic lakes.展开更多
Although biomass variations in Microcystis and microcystin have been widely reported,few studies have addressed whether different trophic states of natural lake water affect the spatial-temporal variations in abundanc...Although biomass variations in Microcystis and microcystin have been widely reported,few studies have addressed whether different trophic states of natural lake water affect the spatial-temporal variations in abundances of microcystin-producing Microcystis in a given bloom.In this study,we used a harmful algal bloom in Chaohu Lake,eastern China,as an example to investigate the mutual relationship between different nutrient states and environmental factors,and the impact on Microcystis.Overall,cyanobacteria and Microcystis were more abundant in the middle and western parts of the lake under high nutrients levels,while in the eastern part,nutrient concentrations were low enough to limit biomass,and their fluctuations affected the contents of toxic Microcystis.Moreover,microcystin concentration was correlated positively to nutrient levels and Microcystis biomass during bloom developing in 2013 from June to August.Temporally,the cellular content of total microcystin was lowest when the bloom peaked in intensity.Our results suggest that lake eutrophication not only results in cyanobacterial blooms,but may also increase the proportion of toxic Microcystis species and their cell-bound MCs contents(i.e.microcystin cell quotas) under mild eutrophication.The present investigation provided molecular evidence for the selection of MC-producing and non-MC-producing genotypes.The current study provides new evidence advocating the monitoring of partitions of large lakes when studying cyanobacteria and toxin-contaminated freshwaters,which will be beneficial for both water agencies and water researchers.展开更多
Many biodiversity indices were used to indicate the biological contamination degree in studies of lake water or seawater. Some were studied on biodiversity comparison for different areas at the same time, or initial s...Many biodiversity indices were used to indicate the biological contamination degree in studies of lake water or seawater. Some were studied on biodiversity comparison for different areas at the same time, or initial structure succession of some aerial lake water systems. The phytoplankton changed with the development of various dominant species. In this study, the dominant species at these stages were Chlorophyta in the beginning stage, Cyanophyta in the second stage, and Xanthophyta in the last stage. Seven of nine biodiversity indices (Margalef's, IE, Shannon-Wiener, Simpson's, McNaughton's, Species and Odds Measure of Diversity) showed their failure to represent the eutrophication trend, and the other two indices (Menhinick's and Monk) exhibited good efficiency to indicate the eutrophication trend for the static landscaping water body.展开更多
By analyzing the enhanced thematic mapper (ETM) images of September 1999, and quality observation data for many consecutive years in several parts of the Donghu Lake in Wuhan, China, the authors discovered a good line...By analyzing the enhanced thematic mapper (ETM) images of September 1999, and quality observation data for many consecutive years in several parts of the Donghu Lake in Wuhan, China, the authors discovered a good linear relation between grey scale (GS) abstracted from ETM b5, b7 images and eutrophication level of the lakes, and extended the study to eight other major lakes in the area of Wu- han by using lake eutrophication models. Based on the in situ monitoring data, we also evaluated the eu- trophication level of the lakes with modified trophic index method brought by M. Aizaki et al. The results of the two methods showed that the most of the lakes were eutrophicated, and even hyper-eutrophicated in some areas. Six of the 8 lakes had very similar trophic state index (TSI) values. Although two of them differed in TSI value, but within an order, while it was different largely from the one by traditional method. The difference of the results between the two methods might have been due to three causative reasons. First, remote sensing technology reflects the overall status of a certain area corresponding to the ETM images in a certain period, but the modified TSI reflects the annual average values of the monitoring spots. Second, the time the ETM images taken is later than that of in situ data. Third, ETM images are affected by clouds, water depth, and suspended matter. In short, remote sensing result agreed greatly with the in situ monitoring data, indicating that remote sensing technology is feasible and effective for moni- toring and evaluating the lake eutrophication in the Wuhan area and it also can be used to evaluate large-scope lake eutrophication.展开更多
Using multi-mesh gillnets and trawls, the fish communities in Dianshan Lake at 6 stations from Oct. 2009 to Jul. 2010 were investigated seasonally to reveal the biodiversity and its spatial and temporal distribution p...Using multi-mesh gillnets and trawls, the fish communities in Dianshan Lake at 6 stations from Oct. 2009 to Jul. 2010 were investigated seasonally to reveal the biodiversity and its spatial and temporal distribution patterns. The long-term changes in their structural characteristics were then analyzed to identify the main infl uencing factors and several measures for lake restoration were put forward. Thirty six species, belonging to 9 family and 30 genera, were collected, amongst which, the order Cypriniformes accounted for 61.1% of the total species number. In terms of importance value, Cypriniformes was the predominant group, Coilia nasus the dominant species, while Cyprinus carpio and Rhinogobius giurinus were the subdominant taxa. The community types did not differ among stations, but between seasons. There were no significant differences between seasons and among stations in species diversity, but richness differed both spatially and seasonally. Along with the process of eutrophication and the drastic reduction of the area colonized by macrophytes from 1959 to 2009–2010, the fish diversity declined markedly, and species numbers of herbivores and piscivores declined proportionately more than those of invertivores, omnivores, and planktivores. The decline of potamophilus and river-lake migratory fish was more marked than those of sedentary, river-sea migratory, and estuarine fishes. Eutrophication concomitant with sharp reduction of macrophyte area and overfishing may be the main reasons for the decline in fish diversity in Dianshan Lake.展开更多
Monitoring algal blooms by optical remote sensing is limited by cloud cover.In this study,synthetic aperture radar(SAR) was deployed with the aim of monitoring cyanobacteria-dominant algal blooms in Taihu Lake in clou...Monitoring algal blooms by optical remote sensing is limited by cloud cover.In this study,synthetic aperture radar(SAR) was deployed with the aim of monitoring cyanobacteria-dominant algal blooms in Taihu Lake in cloudy weather.The study shows that dark regions in the SAR images caused by cyanobacterial blooms damped the microwave backscatter of the lake surface and were consistent with the regions of algal blooms in quasi-synchronous optical images,confirming the applicability of SAR for detection of surface blooms.Low backscatter may also be associated with other factors such as low wind speeds,resulting in interference when monitoring algal blooms using SAR data alone.After feature extraction and selection,the dark regions were classified by the support vector machine method with an overall accuracy of 67.74%.SAR can provide a reference point for monitoring cyanobacterial blooms in the lake,particularly when weather is not suitable for optical remote sensing.Multi-polarization and multi-band SAR can be considered for use in the future to obtain more accurate information regarding algal blooms from SAR data.展开更多
Whether it is necessary to reduce nitrogen(N) and/or phosphorus(P) input to mitigate lake eutrophication is controversial. The controversy stems mainly from differences in time and space in previous studies that suppo...Whether it is necessary to reduce nitrogen(N) and/or phosphorus(P) input to mitigate lake eutrophication is controversial. The controversy stems mainly from differences in time and space in previous studies that support the contrasting ideas. To test the response of phytoplankton to various combinations of nutrient control strategies in mesocosms and the possibility of reflecting the conditions in natural ecosystems with short-term experiments, a 9-month experiment was carried out in eight 800-L tanks with four nutrient level combinations(+N+P,-N+P, +N-P, and-N-P), with an 18-month whole-ecosystem experiment in eight ~800-m^2 ponds as the reference. Phytoplankton abundance was determined by P not N, regardless of the initial TN/TP level, which was in contrast to the nutrient limitation predicted by the N/P theory. Net natural N inputs were calculated to be 4.9, 6.8, 1.5, and 3.0 g in treatments +N+P,-N+P, +N-P, and-N-P, respectively, suggesting that N deficiency and P addition may promote natural N inputs to support phytoplankton development. However, the compensation process was slow, as suggested by an observed increase in TN after 3 weeks in-N+P and 2 months in-N-P in the tank experiment, and after 3 months in-N?+P and ~3 months in-N-P in our pond experiment. Obviously, such a slow process cannot be simulated in short-term experiments. The natural N inputs cannot be explained by planktonic N-fixation because N-fixing cyanobacteria were scarce, which was probably because there was a limited pool of species in the tanks. Therefore, based on our results we argue that extrapolating short-term, small-scale experiments to large natural ecosystems does not give reliable, accurate results.展开更多
基金Project supported by the National Key Basic Research Support Foundation (NKBRSF) of China (No. 2002CB412304) the Chinese Academy of Sciences (No. KZCX1-SW-Ⅱ-32).
文摘A microtiter plate assay (MPA) for soluble reactive phosphorus (SRP) was applied to samples in overlying water and pore water as well as in three forms of environmental soil test phosphorus (P) types: water soluble phosphorus (WSP),diluted calcium chloride extractable phosphorus (PCaCl2), and Olsen-P in the sediments of Taihu Lake, China, where potential P release in response to pH was analyzed. MPA for rapid P analysis was shown to be promising when applied on samples of natural water and sediment extracts. Concentrations of WSP and PCaCl2 in the sediments were much lower than those of Olsen-P. Olsen-P levels in the littoral sediments along the north coast of Meiliang Bay in Taihu Lake (80to 140 mg kg-1) were much higher than those in the mouth of the bay (less than 50 mg kg-1). The risk of P release in the mouth area of Meiliang Bay was lower than that in the north littoral zone with a risk of sediment P release induced by pH increases.
基金Projects KZCX1-SW-12 supported by the Key Project of CAS 2002AA601011-04 by the Hi-tech Research and Development Program of China
文摘Aquatic macrophytic growth and its distribution in eutrophic lakes are described in relation to its sediment type and irradiance. Hence, it is necessary to characterize the response of macrophytic growth to lake sediment and its re-suspension. We conducted two independent experiments to measure the effects of sediment and its re-suspension on the growth of Vallisneria asiatica Miki. Based on our study, we conclude that the amount of total biomass of V. asiatica is related, in important ways, to the type of sediment and its re-suspension. According to our comparison, plants and biomass cultured on lake sediments are 2.1 times and 1.5 times greater than that on brown clay from nearby places beside Lake Taihu. The number of plants and the amount of biomass in a turbid water column are smaller than those in a clear water column. The results indicate that the nutrient-rich lake sediments have promoted the growth of V. asiatica, while the re-suspension of the sediment leads to a high turbid level and low light penetration which has a negative impact on the growth of V. asiatica.
基金Supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China(No.2012ZX07101-007-01)the Collaborative Innovation Center of Technology and Material of Water Treatment
文摘Lake Gehu is a severely eutrophic lake in southeast China. A series of restoration measures have been implemented since 2009 in northern Lake Gehu. This study compared aquatic plants, water quality, sediment, and phytoplankton between restoration and control areas to investigate the effect of restoration measures. The results demonstrated that aquatic macrophyte coverage increased from 0% to 10.6%; mean TP, TN, and CODm concentrations decreased by 50.0%, 42.4%, and 40.8%, respectively, compared with those before the measures were carried out; the mean Secchi depth (SD) increased to 42.5 cm, which is 1.4 times higher than that before restoration; the mean euphotic depth (Zeu) in the summer increased from 91 to 130 cm; the mean chl a concentration decreased from 34.8 to 20.2 μg/L, compared with that before restoration; the Shannon-Wiener index of phytoplankton increased by 28.7%. The mean TP and TN concentrations in sediments decreased by 63.8% and 52.4%, respectively, compared with that before dredging. These results indicate that the restoration in northern Lake Gehu was effective. To complete the transformation from an algae- to a macrophyte-stable state within the region, further measures must be adopted. This restoration of a eutrophic lake can serve as a reference for similar eutrophic lakes.
基金Supported by the Major Science and Technology Program for Water Pollution Control and Treatment(Nos.2012ZX07103-004-02,2015ZX07204002)the Henan Institute of Engineering Innovation Team Building Program(No.CXTD2014005)+2 种基金the Henan Province Science Projects for Colleges and Universities(No.15A610011)the City Science and Technology Project(No.20140659)the Fund Project for Doctor(No.D2014009)
文摘Although biomass variations in Microcystis and microcystin have been widely reported,few studies have addressed whether different trophic states of natural lake water affect the spatial-temporal variations in abundances of microcystin-producing Microcystis in a given bloom.In this study,we used a harmful algal bloom in Chaohu Lake,eastern China,as an example to investigate the mutual relationship between different nutrient states and environmental factors,and the impact on Microcystis.Overall,cyanobacteria and Microcystis were more abundant in the middle and western parts of the lake under high nutrients levels,while in the eastern part,nutrient concentrations were low enough to limit biomass,and their fluctuations affected the contents of toxic Microcystis.Moreover,microcystin concentration was correlated positively to nutrient levels and Microcystis biomass during bloom developing in 2013 from June to August.Temporally,the cellular content of total microcystin was lowest when the bloom peaked in intensity.Our results suggest that lake eutrophication not only results in cyanobacterial blooms,but may also increase the proportion of toxic Microcystis species and their cell-bound MCs contents(i.e.microcystin cell quotas) under mild eutrophication.The present investigation provided molecular evidence for the selection of MC-producing and non-MC-producing genotypes.The current study provides new evidence advocating the monitoring of partitions of large lakes when studying cyanobacteria and toxin-contaminated freshwaters,which will be beneficial for both water agencies and water researchers.
基金Sponsored by the National Basic Research Program of China (Grant No.2004CB418505)
文摘Many biodiversity indices were used to indicate the biological contamination degree in studies of lake water or seawater. Some were studied on biodiversity comparison for different areas at the same time, or initial structure succession of some aerial lake water systems. The phytoplankton changed with the development of various dominant species. In this study, the dominant species at these stages were Chlorophyta in the beginning stage, Cyanophyta in the second stage, and Xanthophyta in the last stage. Seven of nine biodiversity indices (Margalef's, IE, Shannon-Wiener, Simpson's, McNaughton's, Species and Odds Measure of Diversity) showed their failure to represent the eutrophication trend, and the other two indices (Menhinick's and Monk) exhibited good efficiency to indicate the eutrophication trend for the static landscaping water body.
基金Supported by Hubei Provincial Comprehensive Investigation of Land Resources Using Remote Sensing Technology Program (No. 0799210014).
文摘By analyzing the enhanced thematic mapper (ETM) images of September 1999, and quality observation data for many consecutive years in several parts of the Donghu Lake in Wuhan, China, the authors discovered a good linear relation between grey scale (GS) abstracted from ETM b5, b7 images and eutrophication level of the lakes, and extended the study to eight other major lakes in the area of Wu- han by using lake eutrophication models. Based on the in situ monitoring data, we also evaluated the eu- trophication level of the lakes with modified trophic index method brought by M. Aizaki et al. The results of the two methods showed that the most of the lakes were eutrophicated, and even hyper-eutrophicated in some areas. Six of the 8 lakes had very similar trophic state index (TSI) values. Although two of them differed in TSI value, but within an order, while it was different largely from the one by traditional method. The difference of the results between the two methods might have been due to three causative reasons. First, remote sensing technology reflects the overall status of a certain area corresponding to the ETM images in a certain period, but the modified TSI reflects the annual average values of the monitoring spots. Second, the time the ETM images taken is later than that of in situ data. Third, ETM images are affected by clouds, water depth, and suspended matter. In short, remote sensing result agreed greatly with the in situ monitoring data, indicating that remote sensing technology is feasible and effective for moni- toring and evaluating the lake eutrophication in the Wuhan area and it also can be used to evaluate large-scope lake eutrophication.
基金Supported by the Science and Technology Commission of Shanghai Municipality(Nos.08DZ1203101,08DZ1203102)the Shanghai University Knowledge Service Platform,Shanghai Ocean University Aquatic Animal Breeding Center(No.ZF1206)the Open Project of Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Certificated by Ministry of Agriculture of China
文摘Using multi-mesh gillnets and trawls, the fish communities in Dianshan Lake at 6 stations from Oct. 2009 to Jul. 2010 were investigated seasonally to reveal the biodiversity and its spatial and temporal distribution patterns. The long-term changes in their structural characteristics were then analyzed to identify the main infl uencing factors and several measures for lake restoration were put forward. Thirty six species, belonging to 9 family and 30 genera, were collected, amongst which, the order Cypriniformes accounted for 61.1% of the total species number. In terms of importance value, Cypriniformes was the predominant group, Coilia nasus the dominant species, while Cyprinus carpio and Rhinogobius giurinus were the subdominant taxa. The community types did not differ among stations, but between seasons. There were no significant differences between seasons and among stations in species diversity, but richness differed both spatially and seasonally. Along with the process of eutrophication and the drastic reduction of the area colonized by macrophytes from 1959 to 2009–2010, the fish diversity declined markedly, and species numbers of herbivores and piscivores declined proportionately more than those of invertivores, omnivores, and planktivores. The decline of potamophilus and river-lake migratory fish was more marked than those of sedentary, river-sea migratory, and estuarine fishes. Eutrophication concomitant with sharp reduction of macrophyte area and overfishing may be the main reasons for the decline in fish diversity in Dianshan Lake.
基金Supported by the High Resolution Earth Observation Systems of National Science and Technology Major Projects(No.05-Y30B02-9001-13/155)the National High Technology Research and Development Program of China(Nos.2012AA12A301,2013AA12A302)the Key Basic Research Project of the Science and Technology Commission of Shanghai Municipality(No.12510502000)
文摘Monitoring algal blooms by optical remote sensing is limited by cloud cover.In this study,synthetic aperture radar(SAR) was deployed with the aim of monitoring cyanobacteria-dominant algal blooms in Taihu Lake in cloudy weather.The study shows that dark regions in the SAR images caused by cyanobacterial blooms damped the microwave backscatter of the lake surface and were consistent with the regions of algal blooms in quasi-synchronous optical images,confirming the applicability of SAR for detection of surface blooms.Low backscatter may also be associated with other factors such as low wind speeds,resulting in interference when monitoring algal blooms using SAR data alone.After feature extraction and selection,the dark regions were classified by the support vector machine method with an overall accuracy of 67.74%.SAR can provide a reference point for monitoring cyanobacterial blooms in the lake,particularly when weather is not suitable for optical remote sensing.Multi-polarization and multi-band SAR can be considered for use in the future to obtain more accurate information regarding algal blooms from SAR data.
基金Supported by the State Key Laboratory of Freshwater Ecology and Biotechnology(Nos.2014FB14,2011FBZ14)Science and Technology Support Program of Hubei Province(No.2015BBA225)the Youth Innovation Association of Chinese Academy of Sciences(No.2014312)to WANG Haijun
文摘Whether it is necessary to reduce nitrogen(N) and/or phosphorus(P) input to mitigate lake eutrophication is controversial. The controversy stems mainly from differences in time and space in previous studies that support the contrasting ideas. To test the response of phytoplankton to various combinations of nutrient control strategies in mesocosms and the possibility of reflecting the conditions in natural ecosystems with short-term experiments, a 9-month experiment was carried out in eight 800-L tanks with four nutrient level combinations(+N+P,-N+P, +N-P, and-N-P), with an 18-month whole-ecosystem experiment in eight ~800-m^2 ponds as the reference. Phytoplankton abundance was determined by P not N, regardless of the initial TN/TP level, which was in contrast to the nutrient limitation predicted by the N/P theory. Net natural N inputs were calculated to be 4.9, 6.8, 1.5, and 3.0 g in treatments +N+P,-N+P, +N-P, and-N-P, respectively, suggesting that N deficiency and P addition may promote natural N inputs to support phytoplankton development. However, the compensation process was slow, as suggested by an observed increase in TN after 3 weeks in-N+P and 2 months in-N-P in the tank experiment, and after 3 months in-N?+P and ~3 months in-N-P in our pond experiment. Obviously, such a slow process cannot be simulated in short-term experiments. The natural N inputs cannot be explained by planktonic N-fixation because N-fixing cyanobacteria were scarce, which was probably because there was a limited pool of species in the tanks. Therefore, based on our results we argue that extrapolating short-term, small-scale experiments to large natural ecosystems does not give reliable, accurate results.