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富营养饮食重塑代谢性疾病易感猪关键组织mRNA剪接模式研究
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作者 夏博策 张凯艺 +6 位作者 苗佳坤 彭焕祺 杨宇 陶聪 吴添文 王彦芳 杨述林 《畜牧兽医学报》 CAS CSCD 北大核心 2022年第8期2537-2547,共11页
旨在探讨富营养饮食对代谢性疾病易感猪关键组织可变剪接(alternative splicing,AS)和剪接因子(splicing factors,SFs)的影响。本研究以健康状态、月龄和体重相同的12头代谢性疾病易感公猪为材料,随机将其分为两组,其中8头猪饲喂高脂高... 旨在探讨富营养饮食对代谢性疾病易感猪关键组织可变剪接(alternative splicing,AS)和剪接因子(splicing factors,SFs)的影响。本研究以健康状态、月龄和体重相同的12头代谢性疾病易感公猪为材料,随机将其分为两组,其中8头猪饲喂高脂高能量饲料2个月作为富营养饮食组(TG-HFHSD),4头猪饲喂正常饲料2个月作为对照饮食组(TG-CD)。对TG-HFHSD和TG-CD的脂肪、肝、肌肉和下丘脑组织进行RNA-seq测序,利用rMATS软件鉴定组间差异AS,对差异剪接基因(differential splicing genes,DSGs)进行GO和KEGG富集分析,并利用DESeq2软件和皮尔逊相关性系数分析肝组织中的SFs。结果显示,脂肪、肝、肌肉和下丘脑中分别鉴定到48279、39536、47888和52210个AS事件,均以SE类型为主,占比在77.09%~78.29%之间。脂肪、肝、肌肉和下丘脑中的DSGs数分别为528、1070、570和600个,其中肝增加的DSGs部分主要是RI和MXE两种类型,分别为177和471个,而其它组织仅为12~28个和99~110个。GO和KEGG富集显示,肝DSGs除富集到细胞结构相关的过程外,还显著富集到可变剪接及mRNA代谢、脂质代谢、DNA损伤及修复、补体与凝血级联等过程。肝中筛选到56个差异表达的SFs,其中CLK1、PGC-1ɑ、USP4等与糖脂代谢和代谢性疾病密切相关。本研究表明,富营养饮食显著增加了肝DSGs,通过剪接调控、糖脂代谢以及DNA损伤来影响肝代谢性疾病的发生,将为肝代谢性疾病早期发生机制研究提供参考。 展开更多
关键词 富营养饮食 代谢性脂肪肝病 代谢性疾病易感猪 可变剪接 剪接因子
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Diet and feeding ecology of invasive icefish Neosalanx taihuensis in Erhai Lake,a Chinese plateau mesoeutrophicated lake 被引量:1
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作者 胡翠林 过龙根 王圣瑞 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第2期372-380,共9页
A comprehensive study of the invasive icefish Neosalanx taihuensis feeding ecology in Erhai Lake was conducted from November 2009 to October 2010. Prey items in the guts of the icefish sampled varied significantly acc... A comprehensive study of the invasive icefish Neosalanx taihuensis feeding ecology in Erhai Lake was conducted from November 2009 to October 2010. Prey items in the guts of the icefish sampled varied significantly according to season. This finding suggests a relationship between fluctuations in available prey in the environment and selective feeding by icefish. N. taihuensis preferred large-sized zooplankton, such as Daphnia and calanoids. Additionally, the gut fullness values differed significantly (P〈0.001) among sampling times. To compare the values at different times, samples were taken over a 24 h period every 2 months for the entire year. The feeding activities of the fish were concentrated either in the morning (8:00) and/or at dusk (20:00), except in September 2010. This finding can be explained primarily by the variation in optimum light intensity. Daily N. taihuensis zooplankton consumption varied significantly, both diurnally and among seasons. The daily consumption values ranged from 0.089 to 0.237 g (wet weight) per 100 g wet fish weight in temperatures between 11.50℃ and 24.68℃. This is the first report of diel feeding periodicity and daily food consumption of icefish in their invaded ecosystems. 展开更多
关键词 dietary composition crustacean zooplankton feeding ecology invasive species Neosalanxtaihuensis
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