为满足环境气溶胶在线进样的要求,改善电感耦合等离子体质谱计(Inductively Coupled Plasma Mass Spectrometry,ICP蛳MS)对粒子中待测核素的探测限,研制出一套具有低流量、低压降特点的狭缝虚拟撞击器,可作为气溶胶在线富集进样系统的...为满足环境气溶胶在线进样的要求,改善电感耦合等离子体质谱计(Inductively Coupled Plasma Mass Spectrometry,ICP蛳MS)对粒子中待测核素的探测限,研制出一套具有低流量、低压降特点的狭缝虚拟撞击器,可作为气溶胶在线富集进样系统的关键部件与ICPˉMS联用。本文介绍了该狭缝虚拟撞击器的研制情况:首先由ICP蛳MS的进样条件,根据经验公式完成了理论设计,确定进样流量为11L/min,强流与弱流的流量比为10,喷射狭缝宽度为1mm,长宽比为10;然后用计算流体动力学软件Fluent R对上述结构内部的流场和粒子运动径迹进行了数值模拟,考察了喷射狭缝与收集狭缝的间距G、收集狭缝的宽度Wc及其进口曲率半径的加工偏差对收集效率的影响,得到了较优化的取值范围:G取1.0～1.2mm,Wc取1.4～1.6mm,此时的切割粒径D50约2.5μm;加工出狭缝虚拟撞击器的原型装置,对其分别用室内空气气溶胶和荧光素钠标记的单分散油酸粒子进行富集性能测量。实测结果表明:空气动力学等效直径在2.5～5μm范围内的粒子浓度得到了明显富集,5μm的粒子的富集因子达到极大值7。展开更多
[目的]评价SH3结构域谷氨酸富集样蛋白3(SH3BGRL3)在非小细胞肺癌中的表达及其与临床病理特征的相关性。[方法]选取2014年10月至2017年12月确诊并接受手术治疗的46例非小细胞肺癌患者的癌组织标本以及距离癌组织5cm以上的癌旁组织,免疫...[目的]评价SH3结构域谷氨酸富集样蛋白3(SH3BGRL3)在非小细胞肺癌中的表达及其与临床病理特征的相关性。[方法]选取2014年10月至2017年12月确诊并接受手术治疗的46例非小细胞肺癌患者的癌组织标本以及距离癌组织5cm以上的癌旁组织,免疫组化染色检测SH3BGRL3相对表达量,荧光定量RT-PCR检测SH3BGRL3 m RNA表达。[结果]癌组织中SH3BGRL3相对表达量、SH3BGRL3 mRNA表达高于癌旁组织(P<0.05)。肿瘤体积≥5cm3、侵及浆膜、病理分期Ⅲ~Ⅳ期、有淋巴结转移患者SH3BGRL3、SH3BGRL3 m RNA相对表达量高于肿瘤体积<5cm3、未及浆膜、病理分期Ⅰ~Ⅱ期、无淋巴结转移患者(P<0.05)。病理分期、SH3BGRL3、SH3BGRL3 mRNA表达是影响非小细胞肺癌患者预后的主要危险因素。[结论]SH3BGRL3在非小细胞肺癌中呈现异常高表达,且与肿瘤体积、浸润程度、病理分期、淋巴结转移等临床病理参数具有一定相关性。SH3BGRL3、SH3BGRL3 m RNA表达是影响非小细胞肺癌患者预后的危险因素。展开更多
A novel cloud-point extraction （CPE） was successfully used in preconcentration of biphenol A （BPA） from aqueous solutions. Majority of BPA is extracted into the surfactant-rich phase. The parameters affecting the ...A novel cloud-point extraction （CPE） was successfully used in preconcentration of biphenol A （BPA） from aqueous solutions. Majority of BPA is extracted into the surfactant-rich phase. The parameters affecting the CPE such as concentration of surfactant and electrolyte, equilibration temperature and time and pH of sample solution were investigated. The samples were analyzed by high-performance liquid chromatography with ultraviolet detection. Under the optimized conditions, preconcentration of 10 mL sample gives a preconcentration factor of 11. The limit of detection （LOD） and limit of quantification （LOQ） are 0.1 μg/L and 0.33 μg/L, respectively. The linear range of the proposed method is 0.2-20 μg/L with correlation coefficients greater than 0.998 7 and the spiking recove6es are 97.96%-100.42%. The interference factor was tested and the extraction mechanism was also investigated. Thus, the developed CPE has proven to be an efficient, green, rapid and inexpensive approach for extraction and preconcentration of BPA from water samples.展开更多
A novel method for on-site determination of trace iron was developed using membrane preconcentration and spectrophotometric detection. Fe（II）-ferrozine complex was reacted with cetyltrimethylammonium bromide （CTAB...A novel method for on-site determination of trace iron was developed using membrane preconcentration and spectrophotometric detection. Fe（II）-ferrozine complex was reacted with cetyltrimethylammonium bromide （CTAB） to form a Fe（II）-ferrozine-CTAB paired compound, which was collected on a membrane by filtration under vacuum. The membrane was immersed in 2 mL of ethanol-nitric acid and the absorbance of the solution measured for quantitative analysis. Various factors affecting the iron collection and determination were investigated. With different sample preconcentration volumes, the range of determination was broadened to 0.5-120 ~tg/L. The detection limit of this method reached 0.19 ktg/L and the recoveries were between 97.2 and 109% when the concentration enrichment was about 45. The relative standard deviation （n = 7） was 1.9% for samples containing 10 ~g/L Fe. Twelve seawater samples were analyzed on-site using the proposed method, and two were also analyzed in inductively coupled plasma mass spectrometry. No significant difference was shown between the two methods by the Student＇s t-test. The method has also been used on-site for iron enrichment experiments with phytoplankton and concluded to be simple, accurate and inexpensive.展开更多
文摘[目的]评价SH3结构域谷氨酸富集样蛋白3(SH3BGRL3)在非小细胞肺癌中的表达及其与临床病理特征的相关性。[方法]选取2014年10月至2017年12月确诊并接受手术治疗的46例非小细胞肺癌患者的癌组织标本以及距离癌组织5cm以上的癌旁组织,免疫组化染色检测SH3BGRL3相对表达量,荧光定量RT-PCR检测SH3BGRL3 m RNA表达。[结果]癌组织中SH3BGRL3相对表达量、SH3BGRL3 mRNA表达高于癌旁组织(P<0.05)。肿瘤体积≥5cm3、侵及浆膜、病理分期Ⅲ~Ⅳ期、有淋巴结转移患者SH3BGRL3、SH3BGRL3 m RNA相对表达量高于肿瘤体积<5cm3、未及浆膜、病理分期Ⅰ~Ⅱ期、无淋巴结转移患者(P<0.05)。病理分期、SH3BGRL3、SH3BGRL3 mRNA表达是影响非小细胞肺癌患者预后的主要危险因素。[结论]SH3BGRL3在非小细胞肺癌中呈现异常高表达,且与肿瘤体积、浸润程度、病理分期、淋巴结转移等临床病理参数具有一定相关性。SH3BGRL3、SH3BGRL3 m RNA表达是影响非小细胞肺癌患者预后的危险因素。
基金Project(20956001) supported by the National Natural Science Foundation of ChinaProject(CX2011B083) supported by Hunan Provincial Innovation Foundation for Postgraduate, ChinaProject(K1104026-11) supported by Project of Changsha Science and Technology Bureau, China
文摘A novel cloud-point extraction （CPE） was successfully used in preconcentration of biphenol A （BPA） from aqueous solutions. Majority of BPA is extracted into the surfactant-rich phase. The parameters affecting the CPE such as concentration of surfactant and electrolyte, equilibration temperature and time and pH of sample solution were investigated. The samples were analyzed by high-performance liquid chromatography with ultraviolet detection. Under the optimized conditions, preconcentration of 10 mL sample gives a preconcentration factor of 11. The limit of detection （LOD） and limit of quantification （LOQ） are 0.1 μg/L and 0.33 μg/L, respectively. The linear range of the proposed method is 0.2-20 μg/L with correlation coefficients greater than 0.998 7 and the spiking recove6es are 97.96%-100.42%. The interference factor was tested and the extraction mechanism was also investigated. Thus, the developed CPE has proven to be an efficient, green, rapid and inexpensive approach for extraction and preconcentration of BPA from water samples.
基金Supported by State Key Laboratory of Marine Environmental Science(MEL)Funds(No.MELRI1001)
文摘A novel method for on-site determination of trace iron was developed using membrane preconcentration and spectrophotometric detection. Fe（II）-ferrozine complex was reacted with cetyltrimethylammonium bromide （CTAB） to form a Fe（II）-ferrozine-CTAB paired compound, which was collected on a membrane by filtration under vacuum. The membrane was immersed in 2 mL of ethanol-nitric acid and the absorbance of the solution measured for quantitative analysis. Various factors affecting the iron collection and determination were investigated. With different sample preconcentration volumes, the range of determination was broadened to 0.5-120 ~tg/L. The detection limit of this method reached 0.19 ktg/L and the recoveries were between 97.2 and 109% when the concentration enrichment was about 45. The relative standard deviation （n = 7） was 1.9% for samples containing 10 ~g/L Fe. Twelve seawater samples were analyzed on-site using the proposed method, and two were also analyzed in inductively coupled plasma mass spectrometry. No significant difference was shown between the two methods by the Student＇s t-test. The method has also been used on-site for iron enrichment experiments with phytoplankton and concluded to be simple, accurate and inexpensive.
