This study estimates direct radiative forcing by tropospheric ozone and all aerosols between the years 1850 and 2000, using the new IPCC AR5 （the Intergovernmental Panel on Climate Change Fifth Assessment Report） em...This study estimates direct radiative forcing by tropospheric ozone and all aerosols between the years 1850 and 2000, using the new IPCC AR5 （the Intergovernmental Panel on Climate Change Fifth Assessment Report） emissions inventories and a fully coupled chemistry-aerosol general circulation model. As compared to the previous Global Emissions Inventory Activity （GEIA） data, that have been commonly used for forcing estimates since 1990, the IPCC AR5 emissions inventories report lower anthropogenic emissions of organic carbon and black carbon aerosols and higher sulfur and NOx emissions. The simulated global and annual mean burdens of sulfate, nitrate, black carbon （BC）, primary organic aerosol （POA）, secondary organic aerosol （SOA）, and ozone were 0.79, 0.35, 0.05, 0.49, 0.34, and 269 Tg, respectively, in the year 1850, and 1.90, 0.90, 0.11, 0.71, 0.32, and 377 Tg, respectively, in the year 2000. The estimated annual mean top of the atmosphere （TOA） direct radiative forcing of all anthropogenic aerosols based on the AR5 emissions inventories is -0.60 W m^-2 on a global mean basis from 1850 to 2000. However, this is -2.40 W m-2 when forcing values are averaged over eastern China （18-45°N and 95-125°E）. The value for tropospheric ozone is 0.17 W m^-1 on a global mean basis and 0.24 W m^-2 over eastern China. Forcing values indicate that the climatic effect of aerosols over eastern China is much more significant than the globally averaged effect.展开更多
AIM： To investigate the relationship between serum paraoxonase （PON1）, AST, ALT, GGT, and arylesterase （AE） activity alterations and the degree of liver damage in patients with chronic hepatitis. METHODS： We stu...AIM： To investigate the relationship between serum paraoxonase （PON1）, AST, ALT, GGT, and arylesterase （AE） activity alterations and the degree of liver damage in patients with chronic hepatitis. METHODS： We studied 34 chronic hepatitis patients and 32 control subjects, aged between 35 and 65 years, in the Department of Infection and Clinical Microbiology at the Firat University School of Medicine. Blood samples were collected from subjects between 8：00 and 10：00 a.m. following a 12-h fast. Baseline and salt-stimulated PON1 activities were measured by the hydrolysis of paraoxon. Phenyl acetate was used as the substrate and formed phenol was measured spectrophotometrically at 270 nm after the addition of a 10-fold diluted serum sample in AE activity measurements. RESULTS： The results of this investigation revealed that the levels of AE activity decreased from 132±52 to 94± 36 （29%）, baseline PON1 activity from 452±112 to 164 ±67 （64%）, salt-stimulated PON1 activity from 746± 394 to 294±220 （61%）, HDL from 58.4±5.1 to 47.2±5.6 （200）, triglyceride from 133±51.2 to 86±34.0 （350）, while a slight increase in the level of LDL （from 163± 54.1 to 177.3±56.0; 9%） and significant increases in the levels of AST （from 29±9.3 to 98±44）, ALP （from 57.2±13.1 to 91±38.1）, ALT （from 27.9±3.32 to 89± 19.1）, GGT （from 24.3±2.10 to 94±48.2）, total bilirubin （from 0.74±0.02 to 1.36±0.06; 84%） and direct bilirubin （from 0.18±0.01 to 0.42±0.04; 133%） were detected. However, the levels of albumin, total protein, cholesterol, and uric acid were almost the same in chronic hepatitis and the control subjects.CONCLUSION： Low PON1 and AE activity may contribute to the increased liver dysfunction in chronic hepatitis patients by reducing the ability of HDL to retard LDL oxidation and might be clinically useful for monitoring the disease of chronic hepatitis.展开更多
The theoretical and practical analysis of reservoir thickness and oil-bearing information of thin reservoirs is performed by using seismic attributes and forward modelling. The results show that thin reservoir can be ...The theoretical and practical analysis of reservoir thickness and oil-bearing information of thin reservoirs is performed by using seismic attributes and forward modelling. The results show that thin reservoir can be recognized using seismic attributes technique when its thickness is less than 1/4 of wavelength. Through analyzing the influence of tuning effect, the relationship between thin layer thickness and tuning amplitude is well revealed. A precise structure interpretation is conducted using relative amplitude preserved high-resolution seismic data. By taking the geologic condition and well data into account, the distribution of oil and gas of HD4 oilfield is analyzed and predicted. based on seismic attributes. The result is helpful to promote the exploration and development in this oilfield.展开更多
Frontal analysis is frequently applied to measuring single or multi-component adsorption isotherms. In this work, the competitive adsorption isotherm data of two enantiomers of tryptophan were obtained by competitive ...Frontal analysis is frequently applied to measuring single or multi-component adsorption isotherms. In this work, the competitive adsorption isotherm data of two enantiomers of tryptophan were obtained by competitive frontal analysis. The stationary phase in the column was silica-immobilized bovine serum albumin (BSA) by the derivative method, and the mobile phase was a phosphate buffer. These isotherm data were fitted by the competitive Bilangmuir model. This model can account for the behavior of both tryptophan enantiomers and these profiles were found to fit the experimental band profiles (square error is 0.999 6). The parameters obtained were used in numerical calculations to predict the band profiles of the racemic mixtures of tryptophan. The equilibrium-dispersive model provides satisfactory prediction, with minor differences between the calculated and the experimental profiles.展开更多
In the present study, the authors attempted to shed a light on the possible relationships between lipid per oxidation markers, serum malondialdehyde （MDA） with the effect of mercury exposure using serum samples obta...In the present study, the authors attempted to shed a light on the possible relationships between lipid per oxidation markers, serum malondialdehyde （MDA） with the effect of mercury exposure using serum samples obtained from 100 mercury exposed dentals due to their occupation. These dentals were divided into two groups according to their occupational period. The third group consists of healthy volunteers that were working outside the mercury factories as a control group. The present results showed that mercury levels increased with the increasing of their occupation period. The highest level was 5.7 μg/dl, compared to the control group which was 0.1 μg/dl., The level of MDA was elevated in the workers and the highest level was found to be 41.00 nmol/dL for workers with longest period compared to the control group 8.6 nmol/dL. Some serum antioxidants such as vitamin A, E and albumin were evaluated and were found to be lower in all workers compared to the control group. The present results showed that Mg levels increased with the increasing of their occupation period and were found to be lower in Zn, Se of all workers compared to the control group.展开更多
Objective: To review the evidence for tamsulosin as medical expulsive therapy in enhancing the effectiveness of distal ureteral stone clearance rate. Methods: We searched Pubmed/Medline, Embase, CBM and the Cochrane L...Objective: To review the evidence for tamsulosin as medical expulsive therapy in enhancing the effectiveness of distal ureteral stone clearance rate. Methods: We searched Pubmed/Medline, Embase, CBM and the Cochrane Library up to October 2011. All randomized controlled trials in which tamsulosin was evaluated with distal ureterolithiasis were eligible for the analysis. Outcome measure assessed was stone clearance rate. Two authors independently assessed study quality and extracted data. All data were analyzed using RevMan 5.1. Results: Thirteen studies involving 1067 participants met the inclusion criteria. Study duration ranged from 7 to 42 d. The pooled analysis showed an improvement of 41% in stone clearance rate of tamsulosin as medical expulsive therapy for distal ureteral calculi (RR=1.41, 95% CI=1.18 to 1.70). According to stone size (6 mm<size<10 mm, 5 mm<size<6 mm, size <5 mm), the pooling effects of tamsulosin were analyzed, with a higher stone expulsion rate obtained than control (RR=1.52, 1.75, 1.05, 95% CI=1.30 to 1.77, 1.25 to 2.45, 0.95 to 1.16, respectively). Adverse effects of tamsulosin, mainly retrograde ejaculation, dizziness and hypotension, were reported in 7 included trials. Conclusion: Treatment with tamsulosin appears to be a safe and effective medical expulsion therapy for distal ureterolithiasis. To make a definite clinical recommendation to use tamsulosin as medical expulsive treatment for distal ureteral calculi, high quality multicentric, randomized, double blinded, controlled trials are necessary to prove its efficacy.展开更多
Perch sweeper fish with scientific name Cobitis taenia is one of the Cobitidae family. Since this kind of fish lives in clean waters and in the high areas of Talar River, it can be an index on water implution. This su...Perch sweeper fish with scientific name Cobitis taenia is one of the Cobitidae family. Since this kind of fish lives in clean waters and in the high areas of Talar River, it can be an index on water implution. This survey on the diet of this specimen is done on two stations （Kaselian and Tajoon） and shallow and high areas of Talar River in Mazandaran （Southern part of Caspian sea） and the duration of this survey is from October 2008 till September 2009. We should mention that fishing was just done by electro-shocker with voltage 300 V. And this fish after fishing is fixation in 10% formalin solution. So 410 perch sweeper fish were examined and the average of relatively length of gut （RLG） was 17.35 cm. The index of vacated intestine （VI） was calculated 43.35% for all samples. From intense of diet points of view （GSI）, the most frequency of full intestines was considered in summer and spring. On the other hand, the smallness of relatively length of gut index （RLG 〈 1） shows that perch sweeper fish＇s diet manner is inclined to herbivority. In the examination of containing of digestive track, overally 10 kinds of foodstuff were known. Based on amount of this index, 2.67% Chironomidae is known as main bait in all sampling period.展开更多
Objective To observe the effect of acupuncture combined with speech therapy on dysarthria after stroke or cerebral trauma. Methods Sixty-one cases of dysarthria were randomly divided into two groups. The observation g...Objective To observe the effect of acupuncture combined with speech therapy on dysarthria after stroke or cerebral trauma. Methods Sixty-one cases of dysarthria were randomly divided into two groups. The observation group （30 cases） was treated with speech therapy and acupuncture at major acupoints Lianquan （廉泉 CV 23）, Jinjin （金津 EX-HN 12）, Yuye （玉夜 EX-HN 13）, Fengchi（风池 GB 20）, Yifeng （翳风 TE 17） and Wangu（完骨 GB 12）, and the control group （31 cases） was treated with speech therapy only. The changes of speech and acoustical indices were evaluated after 9 weeks treatment. Results The word articulation and the correct rate of writings in the two groups were significantly increased after treatment （both P〈0.01）. The total effective rate of 96.7%（29/30） in the observation group was superior to that of 67.7%（21/31） in the control group （P〈0.01）. The maximum phonation time （MPT） tested by a phonolaryngeal aeromechanical analyzer was significantly prolonged in both the observation group （12 cases） and the control group （11 cases） （both P〈0.01）, the improvement in the observation group being more obvious （P〈0.01）. Conclusion Acupuncture combined with speech therapy can improve the speech and acoustic level in the patient of dysarthria.展开更多
A convective assembly technique at the micron scale analogous to the writing action of a ＂pipette pen＂ has been developed for the linear assembly of gold nanoparticle strips with micron scale width and millimeter sc...A convective assembly technique at the micron scale analogous to the writing action of a ＂pipette pen＂ has been developed for the linear assembly of gold nanoparticle strips with micron scale width and millimeter scale length for surface enhanced Raman scattering （SERS）. The arrays with interparticle gaps smaller than 3 nm are hexagonally stacked in the vicinity of the pipette tip. Variable numbers of stacked layers and clean surfaces of the assembled nanoparticles are obtained by optimizing the velocity of the pipette tip. The SERS properties of tile assembled nanoparticle arrays rely on their stacking number and surface cleanliness.展开更多
The inflammatory cytokine interleukin-1 beta （IL-1β） plays a key role in the process of intervertebral disc degenera- tion （IVDD）. In the present study, we aimed to evaluate the effect of pharmaco-serum of ＂Taor...The inflammatory cytokine interleukin-1 beta （IL-1β） plays a key role in the process of intervertebral disc degenera- tion （IVDD）. In the present study, we aimed to evaluate the effect of pharmaco-serum of ＂Taoren-Honghua-herb pair＂ on IL-1β- induced chondrocyte degeneration in vitro. Taoren （Semen persicae） and Honghua （Safflower carthamus） were administered to the rats, and the pharmaco-serum was collected and prepared. Chondrocytes of the third passage, isolated from the rat＇s vertebral endplates, were treated by standard medium only （Group NC）, IL-1β （Group IL） or combination of IL-1β and pharmaco-serum （Group TRHH）. Cell proliferation and apoptosis were determined, and the expression of aggrecan, Col2ul, Coll0ul, IL-6 and SOX9 at the mRNA level in chondrocytes was quantified by real-time PCR. Immunohistochemistry staining of type II and X collagen and Safranine O staining were also used to evaluate the chondrocytes. Compared with the Group NC, IL-1β treatment inhibited the cell proliferation and induced the cell apoptosis （P〈0.05）, and the expression of aggrecan, Col2αl and SOX9 at the mRNA level was down-regulated. In contrast, the expression of Coll0ul and IL-6 was up-regulated after IL-1β treatment （P〈0.05）. Meanwhile, the immune-staining of type II collagen and Safranine O staining were decreased, while the staining of type X collagen was increased. Compared with the Group IL, cell proliferation was increased, and apoptosis of chondrocytes was decreased when cells were treated with the pharmaco-semm of TRHH-herb pair （P〈0.05）. The expression of aggrecan, Col2cd and SOX9 at the mRNA level was up-regulated, while that of Coll0cd and IL-6 was down-regulated （P〈0.05）. Saffanine O staining also showed increased positive staining （P〈0.05）. Taken together, the treatment of pharmaco-serum of TRHH-herb pair could prevent endplate chondrocyte degeneration induced by IL-1β.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos.90711004 and40825016)the Chinese Academy of Sciences (Grant Nos.KZCX2-YW-Q1 and KZCX2-YW-Q11-03)
文摘This study estimates direct radiative forcing by tropospheric ozone and all aerosols between the years 1850 and 2000, using the new IPCC AR5 （the Intergovernmental Panel on Climate Change Fifth Assessment Report） emissions inventories and a fully coupled chemistry-aerosol general circulation model. As compared to the previous Global Emissions Inventory Activity （GEIA） data, that have been commonly used for forcing estimates since 1990, the IPCC AR5 emissions inventories report lower anthropogenic emissions of organic carbon and black carbon aerosols and higher sulfur and NOx emissions. The simulated global and annual mean burdens of sulfate, nitrate, black carbon （BC）, primary organic aerosol （POA）, secondary organic aerosol （SOA）, and ozone were 0.79, 0.35, 0.05, 0.49, 0.34, and 269 Tg, respectively, in the year 1850, and 1.90, 0.90, 0.11, 0.71, 0.32, and 377 Tg, respectively, in the year 2000. The estimated annual mean top of the atmosphere （TOA） direct radiative forcing of all anthropogenic aerosols based on the AR5 emissions inventories is -0.60 W m^-2 on a global mean basis from 1850 to 2000. However, this is -2.40 W m-2 when forcing values are averaged over eastern China （18-45°N and 95-125°E）. The value for tropospheric ozone is 0.17 W m^-1 on a global mean basis and 0.24 W m^-2 over eastern China. Forcing values indicate that the climatic effect of aerosols over eastern China is much more significant than the globally averaged effect.
文摘AIM： To investigate the relationship between serum paraoxonase （PON1）, AST, ALT, GGT, and arylesterase （AE） activity alterations and the degree of liver damage in patients with chronic hepatitis. METHODS： We studied 34 chronic hepatitis patients and 32 control subjects, aged between 35 and 65 years, in the Department of Infection and Clinical Microbiology at the Firat University School of Medicine. Blood samples were collected from subjects between 8：00 and 10：00 a.m. following a 12-h fast. Baseline and salt-stimulated PON1 activities were measured by the hydrolysis of paraoxon. Phenyl acetate was used as the substrate and formed phenol was measured spectrophotometrically at 270 nm after the addition of a 10-fold diluted serum sample in AE activity measurements. RESULTS： The results of this investigation revealed that the levels of AE activity decreased from 132±52 to 94± 36 （29%）, baseline PON1 activity from 452±112 to 164 ±67 （64%）, salt-stimulated PON1 activity from 746± 394 to 294±220 （61%）, HDL from 58.4±5.1 to 47.2±5.6 （200）, triglyceride from 133±51.2 to 86±34.0 （350）, while a slight increase in the level of LDL （from 163± 54.1 to 177.3±56.0; 9%） and significant increases in the levels of AST （from 29±9.3 to 98±44）, ALP （from 57.2±13.1 to 91±38.1）, ALT （from 27.9±3.32 to 89± 19.1）, GGT （from 24.3±2.10 to 94±48.2）, total bilirubin （from 0.74±0.02 to 1.36±0.06; 84%） and direct bilirubin （from 0.18±0.01 to 0.42±0.04; 133%） were detected. However, the levels of albumin, total protein, cholesterol, and uric acid were almost the same in chronic hepatitis and the control subjects.CONCLUSION： Low PON1 and AE activity may contribute to the increased liver dysfunction in chronic hepatitis patients by reducing the ability of HDL to retard LDL oxidation and might be clinically useful for monitoring the disease of chronic hepatitis.
文摘The theoretical and practical analysis of reservoir thickness and oil-bearing information of thin reservoirs is performed by using seismic attributes and forward modelling. The results show that thin reservoir can be recognized using seismic attributes technique when its thickness is less than 1/4 of wavelength. Through analyzing the influence of tuning effect, the relationship between thin layer thickness and tuning amplitude is well revealed. A precise structure interpretation is conducted using relative amplitude preserved high-resolution seismic data. By taking the geologic condition and well data into account, the distribution of oil and gas of HD4 oilfield is analyzed and predicted. based on seismic attributes. The result is helpful to promote the exploration and development in this oilfield.
文摘Frontal analysis is frequently applied to measuring single or multi-component adsorption isotherms. In this work, the competitive adsorption isotherm data of two enantiomers of tryptophan were obtained by competitive frontal analysis. The stationary phase in the column was silica-immobilized bovine serum albumin (BSA) by the derivative method, and the mobile phase was a phosphate buffer. These isotherm data were fitted by the competitive Bilangmuir model. This model can account for the behavior of both tryptophan enantiomers and these profiles were found to fit the experimental band profiles (square error is 0.999 6). The parameters obtained were used in numerical calculations to predict the band profiles of the racemic mixtures of tryptophan. The equilibrium-dispersive model provides satisfactory prediction, with minor differences between the calculated and the experimental profiles.
文摘In the present study, the authors attempted to shed a light on the possible relationships between lipid per oxidation markers, serum malondialdehyde （MDA） with the effect of mercury exposure using serum samples obtained from 100 mercury exposed dentals due to their occupation. These dentals were divided into two groups according to their occupational period. The third group consists of healthy volunteers that were working outside the mercury factories as a control group. The present results showed that mercury levels increased with the increasing of their occupation period. The highest level was 5.7 μg/dl, compared to the control group which was 0.1 μg/dl., The level of MDA was elevated in the workers and the highest level was found to be 41.00 nmol/dL for workers with longest period compared to the control group 8.6 nmol/dL. Some serum antioxidants such as vitamin A, E and albumin were evaluated and were found to be lower in all workers compared to the control group. The present results showed that Mg levels increased with the increasing of their occupation period and were found to be lower in Zn, Se of all workers compared to the control group.
文摘Objective: To review the evidence for tamsulosin as medical expulsive therapy in enhancing the effectiveness of distal ureteral stone clearance rate. Methods: We searched Pubmed/Medline, Embase, CBM and the Cochrane Library up to October 2011. All randomized controlled trials in which tamsulosin was evaluated with distal ureterolithiasis were eligible for the analysis. Outcome measure assessed was stone clearance rate. Two authors independently assessed study quality and extracted data. All data were analyzed using RevMan 5.1. Results: Thirteen studies involving 1067 participants met the inclusion criteria. Study duration ranged from 7 to 42 d. The pooled analysis showed an improvement of 41% in stone clearance rate of tamsulosin as medical expulsive therapy for distal ureteral calculi (RR=1.41, 95% CI=1.18 to 1.70). According to stone size (6 mm<size<10 mm, 5 mm<size<6 mm, size <5 mm), the pooling effects of tamsulosin were analyzed, with a higher stone expulsion rate obtained than control (RR=1.52, 1.75, 1.05, 95% CI=1.30 to 1.77, 1.25 to 2.45, 0.95 to 1.16, respectively). Adverse effects of tamsulosin, mainly retrograde ejaculation, dizziness and hypotension, were reported in 7 included trials. Conclusion: Treatment with tamsulosin appears to be a safe and effective medical expulsion therapy for distal ureterolithiasis. To make a definite clinical recommendation to use tamsulosin as medical expulsive treatment for distal ureteral calculi, high quality multicentric, randomized, double blinded, controlled trials are necessary to prove its efficacy.
文摘Perch sweeper fish with scientific name Cobitis taenia is one of the Cobitidae family. Since this kind of fish lives in clean waters and in the high areas of Talar River, it can be an index on water implution. This survey on the diet of this specimen is done on two stations （Kaselian and Tajoon） and shallow and high areas of Talar River in Mazandaran （Southern part of Caspian sea） and the duration of this survey is from October 2008 till September 2009. We should mention that fishing was just done by electro-shocker with voltage 300 V. And this fish after fishing is fixation in 10% formalin solution. So 410 perch sweeper fish were examined and the average of relatively length of gut （RLG） was 17.35 cm. The index of vacated intestine （VI） was calculated 43.35% for all samples. From intense of diet points of view （GSI）, the most frequency of full intestines was considered in summer and spring. On the other hand, the smallness of relatively length of gut index （RLG 〈 1） shows that perch sweeper fish＇s diet manner is inclined to herbivority. In the examination of containing of digestive track, overally 10 kinds of foodstuff were known. Based on amount of this index, 2.67% Chironomidae is known as main bait in all sampling period.
基金Supported by Scientific Research Project of China Rehabilitation Research Center:2006-23
文摘Objective To observe the effect of acupuncture combined with speech therapy on dysarthria after stroke or cerebral trauma. Methods Sixty-one cases of dysarthria were randomly divided into two groups. The observation group （30 cases） was treated with speech therapy and acupuncture at major acupoints Lianquan （廉泉 CV 23）, Jinjin （金津 EX-HN 12）, Yuye （玉夜 EX-HN 13）, Fengchi（风池 GB 20）, Yifeng （翳风 TE 17） and Wangu（完骨 GB 12）, and the control group （31 cases） was treated with speech therapy only. The changes of speech and acoustical indices were evaluated after 9 weeks treatment. Results The word articulation and the correct rate of writings in the two groups were significantly increased after treatment （both P〈0.01）. The total effective rate of 96.7%（29/30） in the observation group was superior to that of 67.7%（21/31） in the control group （P〈0.01）. The maximum phonation time （MPT） tested by a phonolaryngeal aeromechanical analyzer was significantly prolonged in both the observation group （12 cases） and the control group （11 cases） （both P〈0.01）, the improvement in the observation group being more obvious （P〈0.01）. Conclusion Acupuncture combined with speech therapy can improve the speech and acoustic level in the patient of dysarthria.
文摘A convective assembly technique at the micron scale analogous to the writing action of a ＂pipette pen＂ has been developed for the linear assembly of gold nanoparticle strips with micron scale width and millimeter scale length for surface enhanced Raman scattering （SERS）. The arrays with interparticle gaps smaller than 3 nm are hexagonally stacked in the vicinity of the pipette tip. Variable numbers of stacked layers and clean surfaces of the assembled nanoparticles are obtained by optimizing the velocity of the pipette tip. The SERS properties of tile assembled nanoparticle arrays rely on their stacking number and surface cleanliness.
基金National Natural Science Foundation of China(Grant No.81273777,81102606,81072831)the National Basic Re search Program of China(Grant No.2010CB530400)the Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT,IRT1270)
文摘The inflammatory cytokine interleukin-1 beta （IL-1β） plays a key role in the process of intervertebral disc degenera- tion （IVDD）. In the present study, we aimed to evaluate the effect of pharmaco-serum of ＂Taoren-Honghua-herb pair＂ on IL-1β- induced chondrocyte degeneration in vitro. Taoren （Semen persicae） and Honghua （Safflower carthamus） were administered to the rats, and the pharmaco-serum was collected and prepared. Chondrocytes of the third passage, isolated from the rat＇s vertebral endplates, were treated by standard medium only （Group NC）, IL-1β （Group IL） or combination of IL-1β and pharmaco-serum （Group TRHH）. Cell proliferation and apoptosis were determined, and the expression of aggrecan, Col2ul, Coll0ul, IL-6 and SOX9 at the mRNA level in chondrocytes was quantified by real-time PCR. Immunohistochemistry staining of type II and X collagen and Safranine O staining were also used to evaluate the chondrocytes. Compared with the Group NC, IL-1β treatment inhibited the cell proliferation and induced the cell apoptosis （P〈0.05）, and the expression of aggrecan, Col2αl and SOX9 at the mRNA level was down-regulated. In contrast, the expression of Coll0ul and IL-6 was up-regulated after IL-1β treatment （P〈0.05）. Meanwhile, the immune-staining of type II collagen and Safranine O staining were decreased, while the staining of type X collagen was increased. Compared with the Group IL, cell proliferation was increased, and apoptosis of chondrocytes was decreased when cells were treated with the pharmaco-semm of TRHH-herb pair （P〈0.05）. The expression of aggrecan, Col2cd and SOX9 at the mRNA level was up-regulated, while that of Coll0cd and IL-6 was down-regulated （P〈0.05）. Saffanine O staining also showed increased positive staining （P〈0.05）. Taken together, the treatment of pharmaco-serum of TRHH-herb pair could prevent endplate chondrocyte degeneration induced by IL-1β.
