PIM-SM(Protocol Independent Multicast-Sparse Mode) is a main multicast routing pro-tocol in the IPv6(Internet Protocol version 6).It can use either a shared tree or a shortest path tree to deliver data packets,consequ...PIM-SM(Protocol Independent Multicast-Sparse Mode) is a main multicast routing pro-tocol in the IPv6(Internet Protocol version 6).It can use either a shared tree or a shortest path tree to deliver data packets,consequently the multicast IP lookup engine requires,in some cases,two searches to get a correct lookup result according to its multicast forwarding rule,and it may result in a new requirement of doubling the lookup speed of the lookup engine.The ordinary method to satisfy this requirement in TCAM(Ternary Content Addressable Memory) based lookup engines is to exploit parallelism among multiple TCAMs.However,traditional parallel methods always induce more re-sources and higher design difficulty.We propose in this paper a novel approach to solve this problem.By arranging multicast forwarding table in class sequence in TCAM and making full use of the intrinsic characteristic of the TCAM,our approach can get the right lookup result with just one search and a single TCAM,while keeping the hardware of lookup engine unchanged.Experimental results have shown that the approach make it possible to satisfy forwarding IPv6 multicast packets at the full link rate of 20 Gb/s with just one TCAM with the current TCAM chip.展开更多
The feature of Ternary Content Addressable Memories(TCAMs) makes them particularly attractive for IP address lookup and packet classification applications in a router system. However,the limitations of TCAMs impede th...The feature of Ternary Content Addressable Memories(TCAMs) makes them particularly attractive for IP address lookup and packet classification applications in a router system. However,the limitations of TCAMs impede their utilization. In this paper,the solutions for decreasing the power consumption and avoiding entry expansion in range matching are addressed. Experimental results demonstrate that the proposed techniques can make some big improvements on the performance of TCAMs in IP address lookup and packet classification.展开更多
Packet classification (PC) has become the main method to support the quality of service and security of network application. And two-dimeusioual prefix packet classification (PPC) is the popular one. This paper analyz...Packet classification (PC) has become the main method to support the quality of service and security of network application. And two-dimeusioual prefix packet classification (PPC) is the popular one. This paper analyzes the problem of ruler conflict, and then presents a TCAM-based two-dimensional PPC algorithm. This algorithm makes use of the parallelism of TCAM to lookup the longest prefix in one instruction cycle. Then it uses a memory image and associated data structures to eliminate the conflicts between rulers, and performs a fast two-dimeusional PPC. Compared with other algorithms, this algorithm has the least time complexity and less space complexity.展开更多
Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect ...Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS),fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that,with UV irradiation,the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.展开更多
This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed o...This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).展开更多
An on-chip electroosmotic(EO) micropump(EOP) was integrated in a microfluidic channel combined with a light-addressable potentiometric sensor(LAPS). The movement of EO flow towards right and left directions can be cle...An on-chip electroosmotic(EO) micropump(EOP) was integrated in a microfluidic channel combined with a light-addressable potentiometric sensor(LAPS). The movement of EO flow towards right and left directions can be clearly observed in the microfluidic channel. The characteristics of photocurrent-time and photocurrent-bias voltage are obtained when buffer solution passes through the sensing region. The results demonstrate that the combination of an on-chip EOP with an LAPS is feasible.展开更多
For enhancing the response of light-addressable potentiometric sensor(LAPS) and further improving its signal-to-noise ratio(SNR),an optical focusing method is adopted.Experimental research and theoretical analysis rev...For enhancing the response of light-addressable potentiometric sensor(LAPS) and further improving its signal-to-noise ratio(SNR),an optical focusing method is adopted.Experimental research and theoretical analysis reveal that the magnitude of responsive signal is increased by optical focusing,and the SNR is improved remarkably.These research results indicate that the optical focusing is an effective approach for improving SNR of LAPS.展开更多
基金Supported by the National High-Tech Research and De-velopment Plan of China (No. 2007AA01Z2a1)the Na-tional Grand Fundamental Research 973 Program of China (No. 2007CB307102)
文摘PIM-SM(Protocol Independent Multicast-Sparse Mode) is a main multicast routing pro-tocol in the IPv6(Internet Protocol version 6).It can use either a shared tree or a shortest path tree to deliver data packets,consequently the multicast IP lookup engine requires,in some cases,two searches to get a correct lookup result according to its multicast forwarding rule,and it may result in a new requirement of doubling the lookup speed of the lookup engine.The ordinary method to satisfy this requirement in TCAM(Ternary Content Addressable Memory) based lookup engines is to exploit parallelism among multiple TCAMs.However,traditional parallel methods always induce more re-sources and higher design difficulty.We propose in this paper a novel approach to solve this problem.By arranging multicast forwarding table in class sequence in TCAM and making full use of the intrinsic characteristic of the TCAM,our approach can get the right lookup result with just one search and a single TCAM,while keeping the hardware of lookup engine unchanged.Experimental results have shown that the approach make it possible to satisfy forwarding IPv6 multicast packets at the full link rate of 20 Gb/s with just one TCAM with the current TCAM chip.
基金the National Natural Science Foundation of China (No.60532030).
文摘The feature of Ternary Content Addressable Memories(TCAMs) makes them particularly attractive for IP address lookup and packet classification applications in a router system. However,the limitations of TCAMs impede their utilization. In this paper,the solutions for decreasing the power consumption and avoiding entry expansion in range matching are addressed. Experimental results demonstrate that the proposed techniques can make some big improvements on the performance of TCAMs in IP address lookup and packet classification.
基金Foundation item: supported by Intel Corporation (No. 9078)
文摘Packet classification (PC) has become the main method to support the quality of service and security of network application. And two-dimeusioual prefix packet classification (PPC) is the popular one. This paper analyzes the problem of ruler conflict, and then presents a TCAM-based two-dimensional PPC algorithm. This algorithm makes use of the parallelism of TCAM to lookup the longest prefix in one instruction cycle. Then it uses a memory image and associated data structures to eliminate the conflicts between rulers, and performs a fast two-dimeusional PPC. Compared with other algorithms, this algorithm has the least time complexity and less space complexity.
基金Project supported by the National Natural Science Foundation of China (Nos. 30627002 and 60725102)the Interdisciplinary Research Foundation of Zhejiang University (No. 2009-15), China
文摘Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS),fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that,with UV irradiation,the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.
基金supported by the National Natural Science Foundation ofChina (No. 60725102)the Natural Science Foundation of Zhejiang Province, China (No. R205505)
文摘This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).
基金supported by the National Natural Science Foundation of China(No.61265006)the China Scholarship Council,and the Graduate School of Biomedical Engineering in Tohoku University
文摘An on-chip electroosmotic(EO) micropump(EOP) was integrated in a microfluidic channel combined with a light-addressable potentiometric sensor(LAPS). The movement of EO flow towards right and left directions can be clearly observed in the microfluidic channel. The characteristics of photocurrent-time and photocurrent-bias voltage are obtained when buffer solution passes through the sensing region. The results demonstrate that the combination of an on-chip EOP with an LAPS is feasible.
基金supported by the National Natural Science Foundation of China(No.61265006)the Specialized Research Fund for the Doctoral Program of Higher Education(No.20126102110031)the Scientific Research Fund of Guangxi Education Department(No.2013YB089)
文摘For enhancing the response of light-addressable potentiometric sensor(LAPS) and further improving its signal-to-noise ratio(SNR),an optical focusing method is adopted.Experimental research and theoretical analysis reveal that the magnitude of responsive signal is increased by optical focusing,and the SNR is improved remarkably.These research results indicate that the optical focusing is an effective approach for improving SNR of LAPS.
