Patients with pemphigus vulgaris (PV) have circulating antidesmoglein (Dsg) 3 immunoglobulin G (IgG) autoantibodies that induce blister formation. We developed an in vitro quantitative assay to evaluate the pathogenic...Patients with pemphigus vulgaris (PV) have circulating antidesmoglein (Dsg) 3 immunoglobulin G (IgG) autoantibodies that induce blister formation. We developed an in vitro quantitative assay to evaluate the pathogenic strength of anti-Dsg3IgG autoantibodies in blister formation. To obtain intercellular adhesion mediated dominantlyby Dsg3,we used primary cultured normal human keratinocytes expressing low level of Dsg2 in the presence of exfoliative toxin A that specifically digests Dsg1. After incubation with various antibodies, monolayers released by dispase were subjected to mechanical stress by pipetting,and the number of cell fragments were counted. When anti-Dsg3 monoclonal antibodies (mAb) obtained from pemphigus model mice were tested, pathogenic AK23 mAb yielded significantly higher number of cell fragments than AK7 or AK20 non-pathogenic mAb. Dissociation scores, defined with AK23 mAb as the positive control, were significantly higher with active stage PVsera(n =10,77.4 ±21.4)thancontrols(n=11,16.0±9.6;p = 0.003). When pair sera obtained from 6 PV patients in active stage and in remission were compared, the dissociation scores reflected well the disease activity as those in active stage were four to 17 times higher than those in remission. When sera from different patients showing similar ELISA scores but different clinical severity were tested (n = 6), the dissociation scores with sera from severe disease activity were significantly higher than those with sera in remission. These findings indicate that this dissociation assay will provide a simple and objective biological method to measure the pathogenic strength of pemphigus autoantibodies.展开更多
Depending on the type of epithelium infected, human papillomaviruses( HPVs)are often subclassified as “ cutaneous” or “ mucosal” types ofHPV. HPVtype 11 is amucosal type of virus and infects the genital tract or l...Depending on the type of epithelium infected, human papillomaviruses( HPVs)are often subclassified as “ cutaneous” or “ mucosal” types ofHPV. HPVtype 11 is amucosal type of virus and infects the genital tract or larynx. Disseminated HPV- 11 infection involving multiple body sites in cases of pemphigus vulgaris (PV) has not been reported. We report here an unusual case of severe pemphigus vulgaris associated with disseminated HPV- 11 infection on the arms, hands, axi llae, chest, abdomen, and both thighs from the genital area, confirmed by the sa me DNA sequence.展开更多
文摘Patients with pemphigus vulgaris (PV) have circulating antidesmoglein (Dsg) 3 immunoglobulin G (IgG) autoantibodies that induce blister formation. We developed an in vitro quantitative assay to evaluate the pathogenic strength of anti-Dsg3IgG autoantibodies in blister formation. To obtain intercellular adhesion mediated dominantlyby Dsg3,we used primary cultured normal human keratinocytes expressing low level of Dsg2 in the presence of exfoliative toxin A that specifically digests Dsg1. After incubation with various antibodies, monolayers released by dispase were subjected to mechanical stress by pipetting,and the number of cell fragments were counted. When anti-Dsg3 monoclonal antibodies (mAb) obtained from pemphigus model mice were tested, pathogenic AK23 mAb yielded significantly higher number of cell fragments than AK7 or AK20 non-pathogenic mAb. Dissociation scores, defined with AK23 mAb as the positive control, were significantly higher with active stage PVsera(n =10,77.4 ±21.4)thancontrols(n=11,16.0±9.6;p = 0.003). When pair sera obtained from 6 PV patients in active stage and in remission were compared, the dissociation scores reflected well the disease activity as those in active stage were four to 17 times higher than those in remission. When sera from different patients showing similar ELISA scores but different clinical severity were tested (n = 6), the dissociation scores with sera from severe disease activity were significantly higher than those with sera in remission. These findings indicate that this dissociation assay will provide a simple and objective biological method to measure the pathogenic strength of pemphigus autoantibodies.
文摘Depending on the type of epithelium infected, human papillomaviruses( HPVs)are often subclassified as “ cutaneous” or “ mucosal” types ofHPV. HPVtype 11 is amucosal type of virus and infects the genital tract or larynx. Disseminated HPV- 11 infection involving multiple body sites in cases of pemphigus vulgaris (PV) has not been reported. We report here an unusual case of severe pemphigus vulgaris associated with disseminated HPV- 11 infection on the arms, hands, axi llae, chest, abdomen, and both thighs from the genital area, confirmed by the sa me DNA sequence.
