目的观察丙泊酚在vlPAG对大鼠伤害性感受的影响及GABAA受体在其中可能的作用。方法Sprague-Daw ley(SD)♀大鼠随机分组,丙泊酚(Propofol,Pro)和荷包牡丹碱(B icucu lline,B ic)采用中脑导水管周围灰质腹外侧区(ven-trolateral portion o...目的观察丙泊酚在vlPAG对大鼠伤害性感受的影响及GABAA受体在其中可能的作用。方法Sprague-Daw ley(SD)♀大鼠随机分组,丙泊酚(Propofol,Pro)和荷包牡丹碱(B icucu lline,B ic)采用中脑导水管周围灰质腹外侧区(ven-trolateral portion of the PAG,vlPAG)注射。行为学实验采用热板法和福尔马林实验,分别以舔后爪潜伏时间(Hot-p latelatency,HPL)和疼痛(累计)评分为指标。免疫组化方法观察丙泊酚在vlPAG对福尔马林单侧足底皮下注射诱发的脊髓背角Fos蛋白表达的影响。结果行为学部分:两种疼痛模型中丙泊酚(10 g.L-1)vlPAG注射引起痛敏(P<0.01)。热板法实验中,丙泊酚vlPAG微量注射的痛敏作用可被相同部位预先注射25 mg.L-1B ic在10和20 m in时间点分别拮抗70%和71%(均P<0.01),在30和40 m in完全拮抗。在福尔马林实验中,丙泊酚vlPAG微量注射使福尔马林疼痛评分增加,此作用可被B ic(25 mg.L-1)在60 m in拮抗57%(P<0.05)。免疫组化部分中,丙泊酚vlPAG微量注射使福尔马林引起的脊髓背角各层FLI阳性细胞数明显增多(P<0.01),B ic vlPAG微量注射(25 mg.L-1)可部分拮抗丙泊酚vlPAG微量注射的作用(P<0.01)。结论在大鼠vlPAG微量注射丙泊酚能产生痛敏作用;GABAA受体部分介导了丙泊酚的以上作用。展开更多
Objective: To explore the effect of oxytocin (OT) of periaqueducta l grey (PAG) of the midbrain on acupuncture analgesia and its relation with endo genous opiates.Methods: Potassium iron iontophoresis method was used ...Objective: To explore the effect of oxytocin (OT) of periaqueducta l grey (PAG) of the midbrain on acupuncture analgesia and its relation with endo genous opiates.Methods: Potassium iron iontophoresis method was used to induce pain, tail flicking was considered as the pain index and the pai n threshold changes were detected before and after microinjection of oxytocin(OT , 2 ng/0.5 μL for each side), anti oxytocin serum (AOTS, 1 ng/0.5 μL), naloxo ne (Nx,5 ng/0.5 μL ), anti β endorphin serum(AEPS), anti metenkephalin seru m (AMEKS) and anti dynorphin A 1～13 serum(ADYNS) into PAG of rats. Results: After bilateral microinjection of OT , the analgesic effect of electroacupuncture (EA) increased significantly in com parison with that of microinjection of same dose of normal saline and presented a dose effect relation (P<0.01). Following microinjection of AOTS , the analgesic effect of EA decreased considerably in comparison with that of normal rabbit se rum (NRS, P<0.01). After microinjection of Nx, the analgesic effec t of EA also d eclined significantly compared with that of normal saline group (P <0.01). After microinjection of Nx and then OT, the effect of EA increased obviously compared with that of Nx+EA group(P<0.01). After microinjection of AEPS or and the n OT, the effect of EA also increased significantly in comparison with that of A EPS+EA and AMEKS+EA group (P<0.01). Following microinjection of AD YNS, the analg esic effect of EA had no significant changes in comparison with NRS group. After microinjection of ADYNS and then OT, the effect of EA increased significantly i n comparison with that of ADYNS+EA group while had no apparent changes in compar ison with that of OT+EA group (P>0.05). The results showed that OT in PAG plays an important role in the EA analgesia, and its effect is independ ent of endogen ous opiate peptides.Conclusion: In the central nervous system, m ultiple analge sic systems may exist simultaneously and OT participates in EA induced analgesic process.展开更多
文摘鼠类为了躲避天敌常常表现出逃跑(flight)或僵立(freezing)的先天防御行为,该行为的产生与中脑导水管周围灰质(periaqueductal gray,PAG)有关。目前,背侧PAG(dorsal PAG,dPAG)对这两种防御行为的神经编码机制尚需要进一步研究。该研究采用模拟天敌迫近的Looming和模拟天敌掠过的sweeping两种视觉刺激范式诱导C57BL/6小鼠先天性防御行为,并将4×4的微电极阵列植入dPAG记录小鼠产生两种防御行为的神经信号,从胞外动作电位(spike)和局部场电位(local field potential,LFP)中分别提取和分析两种防御行为的神经编码特征。
文摘目的观察丙泊酚在vlPAG对大鼠伤害性感受的影响及GABAA受体在其中可能的作用。方法Sprague-Daw ley(SD)♀大鼠随机分组,丙泊酚(Propofol,Pro)和荷包牡丹碱(B icucu lline,B ic)采用中脑导水管周围灰质腹外侧区(ven-trolateral portion of the PAG,vlPAG)注射。行为学实验采用热板法和福尔马林实验,分别以舔后爪潜伏时间(Hot-p latelatency,HPL)和疼痛(累计)评分为指标。免疫组化方法观察丙泊酚在vlPAG对福尔马林单侧足底皮下注射诱发的脊髓背角Fos蛋白表达的影响。结果行为学部分:两种疼痛模型中丙泊酚(10 g.L-1)vlPAG注射引起痛敏(P<0.01)。热板法实验中,丙泊酚vlPAG微量注射的痛敏作用可被相同部位预先注射25 mg.L-1B ic在10和20 m in时间点分别拮抗70%和71%(均P<0.01),在30和40 m in完全拮抗。在福尔马林实验中,丙泊酚vlPAG微量注射使福尔马林疼痛评分增加,此作用可被B ic(25 mg.L-1)在60 m in拮抗57%(P<0.05)。免疫组化部分中,丙泊酚vlPAG微量注射使福尔马林引起的脊髓背角各层FLI阳性细胞数明显增多(P<0.01),B ic vlPAG微量注射(25 mg.L-1)可部分拮抗丙泊酚vlPAG微量注射的作用(P<0.01)。结论在大鼠vlPAG微量注射丙泊酚能产生痛敏作用;GABAA受体部分介导了丙泊酚的以上作用。
文摘Objective: To explore the effect of oxytocin (OT) of periaqueducta l grey (PAG) of the midbrain on acupuncture analgesia and its relation with endo genous opiates.Methods: Potassium iron iontophoresis method was used to induce pain, tail flicking was considered as the pain index and the pai n threshold changes were detected before and after microinjection of oxytocin(OT , 2 ng/0.5 μL for each side), anti oxytocin serum (AOTS, 1 ng/0.5 μL), naloxo ne (Nx,5 ng/0.5 μL ), anti β endorphin serum(AEPS), anti metenkephalin seru m (AMEKS) and anti dynorphin A 1～13 serum(ADYNS) into PAG of rats. Results: After bilateral microinjection of OT , the analgesic effect of electroacupuncture (EA) increased significantly in com parison with that of microinjection of same dose of normal saline and presented a dose effect relation (P<0.01). Following microinjection of AOTS , the analgesic effect of EA decreased considerably in comparison with that of normal rabbit se rum (NRS, P<0.01). After microinjection of Nx, the analgesic effec t of EA also d eclined significantly compared with that of normal saline group (P <0.01). After microinjection of Nx and then OT, the effect of EA increased obviously compared with that of Nx+EA group(P<0.01). After microinjection of AEPS or and the n OT, the effect of EA also increased significantly in comparison with that of A EPS+EA and AMEKS+EA group (P<0.01). Following microinjection of AD YNS, the analg esic effect of EA had no significant changes in comparison with NRS group. After microinjection of ADYNS and then OT, the effect of EA increased significantly i n comparison with that of ADYNS+EA group while had no apparent changes in compar ison with that of OT+EA group (P>0.05). The results showed that OT in PAG plays an important role in the EA analgesia, and its effect is independ ent of endogen ous opiate peptides.Conclusion: In the central nervous system, m ultiple analge sic systems may exist simultaneously and OT participates in EA induced analgesic process.
