In order to speed underwater launch of minor-caliber weapons,a sealing device can be set in front of underwater muzzle to separate water,preventing the muzzle from water immersion.By establishing and simplifying the m...In order to speed underwater launch of minor-caliber weapons,a sealing device can be set in front of underwater muzzle to separate water,preventing the muzzle from water immersion.By establishing and simplifying the model of underwater weapon sealing device and unstructured mesh computing domain model based on computational fluid dynamics(CFD),dynamic mesh and user defined function(UDF),the N-S equation is solved and the numerical analysis and calculation of the complex two-phase flow inside the sealing device are carried out.The results show that the gas discharged from the sealing device is conducive to the formation of the projectile supercavity.When the projectile is launched at 5munder water,the shock wave before and after the projectile has impact on the box body up to 100 MPa,therefore the sealing device must be strong enough.The research results have the vital significance to the design of underwater weapon sealing device and the formation of the projectile supercavitation.展开更多
This paper studies the close-contact melting around a moving, horizontal elliptical heat source thatmelts its way through a phase change material under its own weight. The heat source velocity andthe volume of the mat...This paper studies the close-contact melting around a moving, horizontal elliptical heat source thatmelts its way through a phase change material under its own weight. The heat source velocity andthe volume of the material melted are obtained by analysis. The effects of compression coefficient andtemperature distribution in the melt are investigated. The results include that for melting around amoving cylindrical heat source.展开更多
A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column (350 mm×4.6 mm, 5 ...A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column (350 mm×4.6 mm, 5 μm). The mobile phase was consisted of methanol-water (58:42, v/v). The flow rate of mobile phase was set at 0.8 mL/min. The UV detection wavelength was 271 nm, and the column temperature was 30 ℃. The linear range of 5-FPE was from 0.8-12.8 μg/mL, r = 0.9999. The RSD of intm-day and inter-day precision were less than 2.97%. The average recovery was from 96.8%-104.6% with RSD less than 2.24%. The method was simple, rapid, accurate, and sensitive. It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation.展开更多
Human serum albumin(HSA)is the most abundant plasma protein and has an inherent ability to target tumor cells.It is an excellent candidate for drug delivery.However,HSA cannot form complex with DNA or RNA,because it i...Human serum albumin(HSA)is the most abundant plasma protein and has an inherent ability to target tumor cells.It is an excellent candidate for drug delivery.However,HSA cannot form complex with DNA or RNA,because it is negatively charged under physiological conditions.In this work,we reported a simple method to prepare HSA/RNA nanoparticles mainly by physical interaction.Firstly,the solution p H is adjusted to 4.0,under which condition HSA is positively charged.It forms complex with RNA via electrostatic interaction.The solution is then heated at 75 oC for 15 min to stabilize the structure and the size of the formed complex.The HSA/RNA nanoparticle prepared by this method has a diameter about 110 nm and a narrow distribution.It is also stable for days under physiological conditions.Cellular essays demonstrate that these particles exhibit a high cellular uptake efficiency and non-toxicity to He La cells.展开更多
In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine (PC)-functionalized poly(butylene succinate) (PBS-PC) was investigated by means of hemolysis, platelet adhesion, protei...In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine (PC)-functionalized poly(butylene succinate) (PBS-PC) was investigated by means of hemolysis, platelet adhesion, protein adsorption and cytotox- icity experiments. The reference materials were poly(butylene succinate) (PBS) and chloroethylphosphoryl functionalized poly(butylene succinate) (PBS-Cl). The hemolysis rates (HR) of the leaching solutions of PBS, PBS-Cl and PBS-PC were all lower than the safe value, and the rate of PBS-PC was reduced to 1.07%. Scanning electron microscopy (SEM) measurements showed that platelet adhesion and aggregation were significant on both PBS and PBS-Cl surface. In contrast, very few platelets were observed on PBS-PC surface. Bicinchoninic acid (BCA) measurements revealed that the adsorption amounts of bovine serum albumin (BSA) and bovine plasma fibrinogen (BPF) on PBS-PC surface were 52% and 72% reduction respectively compared with those on PBS surface. Moreover, non-cytotoxicity of both PBS-PC particles and its leaching solution was sug- gested by MTT assay using mouse L929 fibroblast cells. All the results demonstrated that the biocompatibility of PBS could be greatly improved by PC end-capping strategy. This PC functionalized polyester may have potential applications in biological environments as a novel carrier for controlled drug release and scaffold for tissue engineering.展开更多
An accurate closed form solution is proposed to estimate camera pose by several mirrored reference object images acquired via a planar mirror under different unknown poses. Compared with state-of-the-art methods, our ...An accurate closed form solution is proposed to estimate camera pose by several mirrored reference object images acquired via a planar mirror under different unknown poses. Compared with state-of-the-art methods, our method is more accurate when there are more than three images and has explicit geometric meanings. This method also properly handles cases in which some of the mirror poses are parallel. The central idea is to minimize an error metric based on all reflections of rotation, which enables the camera rotation to be estimated directly by SVD of sum of mirrored camera rotations. After that, the camera translation is computed by solving a large system of linear equations to minimize object space collinearity error. Both synthesized data and real data experiments show the advantages of our approach.展开更多
The studies on the mechanisms and performances of the mechanical seals in reactor coolant pumps are very important for the safe operations of the pressurized water reactor power plants. Based on the hydrostatic mechan...The studies on the mechanisms and performances of the mechanical seals in reactor coolant pumps are very important for the safe operations of the pressurized water reactor power plants. Based on the hydrostatic mechanical seal in reactor coolant pumps, an analytical fluid-solid strong-interaction model is proposed in this paper. According to the design features and operafional principles of the seal, an analytical method to calculate the mechanical deformation of the seal assembly is developed based on the ring deformation theory. A strong-interaction algorithm combining the analysis of the mechanical deformation of the seal assembly and flow field between the seal faceplates is utilized, in which the three kinds of equations including the fluid domain, solid domain and coupling action are constituted in the same equations set and all the variables are solved simul- taneously. So the analytical fluid-solid strong-interaction model used for the seal is built. Moreover, the model is verified by the experimental results. Based on the model, the design parameters of the seal are studied. Two different conditions of the general case and fixed seal leakage rate are discussed respectively, and the regularities that the seal behaviors are affected by the parameters of the holding screws on the clamp rings and seal faceplates are obtained. The research results can provide a theoretical basis for performance analysis, design and assemblage of the seal. Compared to the numerical methods, the proposed model has the unique advantages of high efficiency, convenience and easy application of constraints.展开更多
基金National Natural Science Foundation of China(No.51175481)
文摘In order to speed underwater launch of minor-caliber weapons,a sealing device can be set in front of underwater muzzle to separate water,preventing the muzzle from water immersion.By establishing and simplifying the model of underwater weapon sealing device and unstructured mesh computing domain model based on computational fluid dynamics(CFD),dynamic mesh and user defined function(UDF),the N-S equation is solved and the numerical analysis and calculation of the complex two-phase flow inside the sealing device are carried out.The results show that the gas discharged from the sealing device is conducive to the formation of the projectile supercavity.When the projectile is launched at 5munder water,the shock wave before and after the projectile has impact on the box body up to 100 MPa,therefore the sealing device must be strong enough.The research results have the vital significance to the design of underwater weapon sealing device and the formation of the projectile supercavitation.
文摘This paper studies the close-contact melting around a moving, horizontal elliptical heat source thatmelts its way through a phase change material under its own weight. The heat source velocity andthe volume of the material melted are obtained by analysis. The effects of compression coefficient andtemperature distribution in the melt are investigated. The results include that for melting around amoving cylindrical heat source.
基金Natural Science Foundation of Gansu Provence (Grant No. 0803RJZA079)Foundation of Herb Medicin Research of Gansu Provence (Grant No. GZK-2009-1)
文摘A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column (350 mm×4.6 mm, 5 μm). The mobile phase was consisted of methanol-water (58:42, v/v). The flow rate of mobile phase was set at 0.8 mL/min. The UV detection wavelength was 271 nm, and the column temperature was 30 ℃. The linear range of 5-FPE was from 0.8-12.8 μg/mL, r = 0.9999. The RSD of intm-day and inter-day precision were less than 2.97%. The average recovery was from 96.8%-104.6% with RSD less than 2.24%. The method was simple, rapid, accurate, and sensitive. It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation.
基金the National Natural Science Foundation of China (21574002)
文摘Human serum albumin(HSA)is the most abundant plasma protein and has an inherent ability to target tumor cells.It is an excellent candidate for drug delivery.However,HSA cannot form complex with DNA or RNA,because it is negatively charged under physiological conditions.In this work,we reported a simple method to prepare HSA/RNA nanoparticles mainly by physical interaction.Firstly,the solution p H is adjusted to 4.0,under which condition HSA is positively charged.It forms complex with RNA via electrostatic interaction.The solution is then heated at 75 oC for 15 min to stabilize the structure and the size of the formed complex.The HSA/RNA nanoparticle prepared by this method has a diameter about 110 nm and a narrow distribution.It is also stable for days under physiological conditions.Cellular essays demonstrate that these particles exhibit a high cellular uptake efficiency and non-toxicity to He La cells.
基金financially supported by the National Natural Science Foundation of China (21004048, 20974087)the Natural Science Foundation of Shaanxi Educational Committe (2010JK886)the Science Foundation of Northwest University (NF0912)
文摘In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine (PC)-functionalized poly(butylene succinate) (PBS-PC) was investigated by means of hemolysis, platelet adhesion, protein adsorption and cytotox- icity experiments. The reference materials were poly(butylene succinate) (PBS) and chloroethylphosphoryl functionalized poly(butylene succinate) (PBS-Cl). The hemolysis rates (HR) of the leaching solutions of PBS, PBS-Cl and PBS-PC were all lower than the safe value, and the rate of PBS-PC was reduced to 1.07%. Scanning electron microscopy (SEM) measurements showed that platelet adhesion and aggregation were significant on both PBS and PBS-Cl surface. In contrast, very few platelets were observed on PBS-PC surface. Bicinchoninic acid (BCA) measurements revealed that the adsorption amounts of bovine serum albumin (BSA) and bovine plasma fibrinogen (BPF) on PBS-PC surface were 52% and 72% reduction respectively compared with those on PBS surface. Moreover, non-cytotoxicity of both PBS-PC particles and its leaching solution was sug- gested by MTT assay using mouse L929 fibroblast cells. All the results demonstrated that the biocompatibility of PBS could be greatly improved by PC end-capping strategy. This PC functionalized polyester may have potential applications in biological environments as a novel carrier for controlled drug release and scaffold for tissue engineering.
基金supported by the National Natural Science Foundation of China(Grant No.11072263)
文摘An accurate closed form solution is proposed to estimate camera pose by several mirrored reference object images acquired via a planar mirror under different unknown poses. Compared with state-of-the-art methods, our method is more accurate when there are more than three images and has explicit geometric meanings. This method also properly handles cases in which some of the mirror poses are parallel. The central idea is to minimize an error metric based on all reflections of rotation, which enables the camera rotation to be estimated directly by SVD of sum of mirrored camera rotations. After that, the camera translation is computed by solving a large system of linear equations to minimize object space collinearity error. Both synthesized data and real data experiments show the advantages of our approach.
基金supported by the National Basic Research Program of China(Grant No.2009CB724304)the Key Research Program of the State Key Laboratory of Tribology of Tsinghua University(Grant No.SKLT08A06)the National Natural Science Foundation of China(Grant No.50975157)
文摘The studies on the mechanisms and performances of the mechanical seals in reactor coolant pumps are very important for the safe operations of the pressurized water reactor power plants. Based on the hydrostatic mechanical seal in reactor coolant pumps, an analytical fluid-solid strong-interaction model is proposed in this paper. According to the design features and operafional principles of the seal, an analytical method to calculate the mechanical deformation of the seal assembly is developed based on the ring deformation theory. A strong-interaction algorithm combining the analysis of the mechanical deformation of the seal assembly and flow field between the seal faceplates is utilized, in which the three kinds of equations including the fluid domain, solid domain and coupling action are constituted in the same equations set and all the variables are solved simul- taneously. So the analytical fluid-solid strong-interaction model used for the seal is built. Moreover, the model is verified by the experimental results. Based on the model, the design parameters of the seal are studied. Two different conditions of the general case and fixed seal leakage rate are discussed respectively, and the regularities that the seal behaviors are affected by the parameters of the holding screws on the clamp rings and seal faceplates are obtained. The research results can provide a theoretical basis for performance analysis, design and assemblage of the seal. Compared to the numerical methods, the proposed model has the unique advantages of high efficiency, convenience and easy application of constraints.