采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取...采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%。通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖。展开更多
A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation...A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation measurement. This HPⅠconsists of Fuc,Glc and Gal in the molar ratios of 0.423∶1∶2.110. Its structural features were investigated by methylation and GC-MS analysis,periodate oxidation-Smith degradation,and partial acid-hydrolysis. In addition, enzymatic degradation experiments and GC analysis were applied. The following structure of the highly branched repeating unit was established: 6→)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→ 2 ↑ 1 2 ↑ 1 2 ↑ 1 Gal-(1→4)-Fuc Glc-(1→3)-Glc 2 Gal-(1→6)-Glc 3 The activities of HPⅠwere tested in mice. All the samples exhibited high effect of prevention and cure against chronic gastricism.展开更多
文摘采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%。通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖。
文摘A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation measurement. This HPⅠconsists of Fuc,Glc and Gal in the molar ratios of 0.423∶1∶2.110. Its structural features were investigated by methylation and GC-MS analysis,periodate oxidation-Smith degradation,and partial acid-hydrolysis. In addition, enzymatic degradation experiments and GC analysis were applied. The following structure of the highly branched repeating unit was established: 6→)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→ 2 ↑ 1 2 ↑ 1 2 ↑ 1 Gal-(1→4)-Fuc Glc-(1→3)-Glc 2 Gal-(1→6)-Glc 3 The activities of HPⅠwere tested in mice. All the samples exhibited high effect of prevention and cure against chronic gastricism.
