目的分析羊口蹄疫灭活疫苗(inactivated food and mouth disease vaccine,IFMDV)分别与小反刍兽疫活疫苗(live attenuated peste des petits ruminants vaccine,LPPRV)及布氏菌活疫苗(live Brucella vaccine,LBV)联合使用的免疫效果。...目的分析羊口蹄疫灭活疫苗(inactivated food and mouth disease vaccine,IFMDV)分别与小反刍兽疫活疫苗(live attenuated peste des petits ruminants vaccine,LPPRV)及布氏菌活疫苗(live Brucella vaccine,LBV)联合使用的免疫效果。方法应用口蹄疫O型、亚洲1型二价灭活疫苗与LPPRV联合免疫山羊,观察山羊不良反应,采用病毒中和试验法和液相阻断ELISA法分别测定山羊血清中小反刍兽疫抗体及口蹄疫抗体水平。应用口蹄疫O型、A型、亚洲1型三价灭活疫苗与LBV(M5株)联合免疫绵羊,观察绵羊的不良反应,采用试管凝集试验及液相阻断ELISA法检测绵羊血清中布氏菌抗体及口蹄疫抗体水平。结果 IFMDV分别与LPPRV及LBV联合免疫山羊及绵羊,均未见明显的不良反应,仅出现一过性体温升高现象;IFMDV(肌肉)和LPPRV(皮下)同时联合免疫,山羊血清中小反刍兽疫抗体水平明显高于单独免疫LPPRV组(P<0.05);IFMDV与LBV联合免疫,绵羊血清中布氏菌抗体水平高于单独免疫LBV组(P<0.05),且联合免疫组抗体阳性率达100%,比单独免疫LBV组提高了13%。结论 IFMDV分别与LPPRV、LBV联合使用具有良好的安全性,且可提高LPPRV及LBV的免疫效果。展开更多
In this study,the decay of maternal peste des petits ruminants virus(PPRV) antibodies in kids born to goats vaccinated with Asian lineage IV PPR vaccine and the efficacy of passive immunity against PPRV was assessed t...In this study,the decay of maternal peste des petits ruminants virus(PPRV) antibodies in kids born to goats vaccinated with Asian lineage IV PPR vaccine and the efficacy of passive immunity against PPRV was assessed to determine the appropriate period for vaccination in kids.Serum samples collected from kids born to vaccinated,unvaccinated and infected goats at different time intervals were tested by PPR competitive ELISA and serum neutralization test(SNT).Maternal antibodies in kids were detectable up to 6 months with a decline trend from the third month onwards and receded below the protective level by the fourth month.The kid with an SN titre of 1:8 at the time of immunization showed significant PPRV specific antibody response(percentage inhibition of 76;SN titers >1:16),when tested on 21 day post-vaccination and was completely protected from infection upon virulent PPRV challenge.Similarly,the kid with 1:8 SN titers was completely protected from PPR infection on active challenge.Therefore,PPR vaccination is recommended in kids,aged 4 months and born to immunized or exposed goats.This could be a suitable period to avoid window of susceptibility in kids to PPRV and the effort to eliminate PPR infection from susceptible populations.展开更多
基金Project Directorate on Animal Disease Monitoring and Surveillance(PD_ADMAS)
文摘In this study,the decay of maternal peste des petits ruminants virus(PPRV) antibodies in kids born to goats vaccinated with Asian lineage IV PPR vaccine and the efficacy of passive immunity against PPRV was assessed to determine the appropriate period for vaccination in kids.Serum samples collected from kids born to vaccinated,unvaccinated and infected goats at different time intervals were tested by PPR competitive ELISA and serum neutralization test(SNT).Maternal antibodies in kids were detectable up to 6 months with a decline trend from the third month onwards and receded below the protective level by the fourth month.The kid with an SN titre of 1:8 at the time of immunization showed significant PPRV specific antibody response(percentage inhibition of 76;SN titers >1:16),when tested on 21 day post-vaccination and was completely protected from infection upon virulent PPRV challenge.Similarly,the kid with 1:8 SN titers was completely protected from PPR infection on active challenge.Therefore,PPR vaccination is recommended in kids,aged 4 months and born to immunized or exposed goats.This could be a suitable period to avoid window of susceptibility in kids to PPRV and the effort to eliminate PPR infection from susceptible populations.
