[ Objective] To clone and analyze the sequence of Adiponectin receptor 1 ( AdipoR1 ) and receptor 2 (AdipoR2) cDNA of Guangxi Bama mini-pig. [Method] The Adiponectin receptors cDNAs were amplified by RT-PCR using ...[ Objective] To clone and analyze the sequence of Adiponectin receptor 1 ( AdipoR1 ) and receptor 2 (AdipoR2) cDNA of Guangxi Bama mini-pig. [Method] The Adiponectin receptors cDNAs were amplified by RT-PCR using skeletal muscle total RNA as template and then ligated into pMD18-T vector after purification. The recombinant pMD18-T vector was transformed into the E. coil DH5α for identification and sequencing. And the results were compared with the cDNA sequence from other species. [Result] The fragments, 1 128 bp and 1 161 bp in size, were amplified by RT-PCR and respectively consistent with the coding sequence of AdipoR1 gene and AdipoR2 gene. The homology analysis showed that the sequences of AdipoR1 gene and AdipoR2 gene were respectively 99.8% and 99.7% homologous to the sequence of domestic pig reported in GenBank with one base and three base missense mutations correspondingly. [ Conclusion] The AdipoR1 gene and AdipoR2. gene were successfully amplified from Guangxi Bama mini-pig, laying the foundation for the further study of the biological function of AdipoR genes and the design of novel drugs with AdipoR as target.展开更多
AIM: To introduce and evaluate a new method to re- pair bile duct defect with a degradable stent and au- tologous tissues. METHODS: Eight Ba-Ma mini-pigs were used in this study, Experimental models with common bile...AIM: To introduce and evaluate a new method to re- pair bile duct defect with a degradable stent and au- tologous tissues. METHODS: Eight Ba-Ma mini-pigs were used in this study, Experimental models with common bile duct (CBD) defect (0,5-1,0 cm segment of CBD resected) were established and then CBD was reconstructed by duct to duct anastomosis with a novel degradable stent made of poly [sebacic acid-co-(1,3-propanediol)-co- (1,2-propanediol)]. In addition, a vascularized greater omentum was placed around the stent and both ends of CBD. Cholangiography via gall bladder was per- formed for each pig at postoperative months 1 and 3 to rule out stent translocation and bile duct stricture. Complete blood count was examined pre- and post- operatively to estimate the inflammatory reaction. Liver enzymes and serum bilirubin were examined pre- and post-operatively to evaluate the liver function. Five pigs were sacrificed at month 3 to evaluate the healing of anastomosis. The other three pigs were raised for one year for long-term observation. RESULTS: All the animals underwent surgery success- fully. There was no intraoperative mortality and no bile leakage during the observation period, The white blood cell counts were only slightly increased on day 14 and month 3 postoperatively compared with that before operation, the difference was not statistically significant (P = 0.652). The plasma level of alanine aminotrans- ferase on day 14 and month 3 postoperatively was also not significantly elevated compared with that before operation (P = 0.810). Nevertheless, the plasma level of y-glutamyl transferase was increased after opera- tion in both groups (P = 0.004), especially 2 wk after operation. The level of serum total bilirubin after opera- tion was not significantly elevated compared with that before operation (P = 0.227), so did the serum direct bilirubin (P = 0.759). By cholangiography yia gall blad- der, we found that the stent maintained its integrity of shape and was stillin situ at month 1, and it disap- peared completely at month 3. No severe CBD dilation and stricture were observed at both months 1 and 3. No pig died during the 3-too postoperative observation period. No sign of necrosis, bile duct stricture, bile leak- age or abdominal abscess was found at reoperation at month 3 postoperatively. Pigs had neither fragments of stent nor stones formed in the CBD. Collagen deposit was observed in the anastomosis by hematoxylin and eosin (HE) and Masson's trichrome stains. No severe cholestasis was observed in liver parenchyma by HE staining. Intestinal obstruction was found in a pig 4 mo after operation, and no bile leakage, bile duct stricture or biliary obstruction were observed in laparotomy. No sign of bile duct stricture or bile leakage was observed in the other two pigs. CONCLUSION: The novel method for repairing bile duct defect yielded a good short-term effect without postoperative bile duct stricture. However, the long- term effect should be further studied.展开更多
To eliminate the grey bias and improve ant-jamming performance of the standard grey-Markov forecasting model,a forecasting model based on wavelet packet decomposition and fuzzy grey Markov(FG-Markov)is proposed consid...To eliminate the grey bias and improve ant-jamming performance of the standard grey-Markov forecasting model,a forecasting model based on wavelet packet decomposition and fuzzy grey Markov(FG-Markov)is proposed considering the characteristics of randomness and nonlinearility of freight volume forecasting.Firstly,based on the data analysis ability of wavelet packet to non-stationary random signal,wavelet packet decomposition is used to improve the analysis ability of data signal by decomposing historical freight volume data into wavelet packet component.On this basis,FG-Markov chain is proposed to obtain the transfer probability matrix of wavelet packet coefficients by introducing fuzzy grey variables,and forecast the freight volume by reconstructing wavelet packet coefficients.Finally,an example of Lanzhou railroad hub is carried out in order to testify the validity and applicability of this forecasting model.Compared with neural network model and other forecasting models,the proposed forecasting model can improve the forecasting accuracy under the same conditions.The forecasting accuracy of wavelet packet decomposition and FG-Markov is not only greater than that of any other single forecasting models,but also superior to that of other traditional combinational forecasting models,which can meet the actual requirements of freight volume forecasting.展开更多
Objective Machado-Joseph disease (MJD), also known as spinocerebellar ataxia type 3 (SCA3), is a dominant neurodegenerative disorder caused by an expansion of the polyglutamine (polyQ) tract in MJD-1 gene produc...Objective Machado-Joseph disease (MJD), also known as spinocerebellar ataxia type 3 (SCA3), is a dominant neurodegenerative disorder caused by an expansion of the polyglutamine (polyQ) tract in MJD-1 gene product, ataxin-3 (AT3). This disease is characterized by the formation of intraneuronal inclusions, but the mechanism underlying their formation is still poorly understood. The present study is to explore the relationship between wild type (WT) AT3 and polyQ expanded AT3. Methods Mouse neuroblastoma (N2a) cells or HEK293 cells were co-transfected with WTAT3 and different truncated forms of expanded AT3. The expressions of WT AT3 and the truncated forms of expanded AT3 were detected by Western blotting, and observed by an inverted fluorescent microscope. The interactions between AT3 and different truncated forms of expanded AT3 were detected by immunoprecipitation and GST pull-down assays. Results Using fluorescent microscope, we observed that the truncated forms of expanded AT3 aggregate in transfected cells, and the full-length WT AT3 is recruited onto the aggregates. However, no aggregates were observed in cells transfected with the truncated forms of WT AT3. Immunoprecipitation and GST pull-down analyses indicate that WT AT3 interacts with the truncated AT3 in a polyQ length-dependent manner. Conclusion WT AT3 deposits in the aggregation that was formed by polyQ expanded AT3, which suggests that the formation of AT3 aggregation may affect the normal function of WT AT3 and increase polyQ protein toxicity in MJD.展开更多
Two hundred and eighty six lines were selected randomly from the tetraploid wheat (Tritieum turgidum L.var Durum) landrace "Safra Ma'an". The whole population with three check cultivars (ACSAD 65, HOURANI 27 and...Two hundred and eighty six lines were selected randomly from the tetraploid wheat (Tritieum turgidum L.var Durum) landrace "Safra Ma'an". The whole population with three check cultivars (ACSAD 65, HOURANI 27 and AMRA) was evaluated at Maru Agriculture Research Station for 16 characters including grain yield per plant. The objectives were to assess the magnitude of phenotypic variations for the traits in "Safra Ma'an" and to evaluate the potential usefulness of some of the traits identified. Results showed wide range of phenotypic variation for several traits. Monomorphism was common for juvenile growth habit, whereas, the rest of the characters exhibited polymorphism in varying degrees. Considering all characters, the average diversity (H') for "Safra Ma'an" landrace was 0.65 ± 0.047. Ten lines were superior to the check (HOURANI 27) for grain yield per plant. Subsequently, the population lines were clustered into six distinct groups at a distance of about 0.55 based on their similarity for all traits, ACSAD 65 and AMRA located in separate clusters, whereas, HOURANI 27 cultivar was presented in cluster with the most lines of "Safra Ma'an". Thirteen lines from the population showed a bluish green cast or glaucousness characters. Glaucous lines had greater kernels per spike. In contrast, this character showed no significant association with grain yield per plant despite the greater grain yield per plant obtained for the glaucous lines. The results are important for the breeding and selection in this land race.展开更多
Entrepreneurship and growth are driving forces in the development of emerging economic systems. In this exploratory study we discuss emerging economies within a South-East Asia context. In particular, we expand on a p...Entrepreneurship and growth are driving forces in the development of emerging economic systems. In this exploratory study we discuss emerging economies within a South-East Asia context. In particular, we expand on a previous study on the East Asian Tiger economies, and introduce the mini-dragon economies of Indonesia, Malaysia, Thailand, and Philippines. We explore the role of various economic indicators and indices entrepreneurship and SMEs as drivers of growth in these emerging economies. Growth factors unique to the region are also identified, and institutional and government interventions are recommended. Furthermore, we elaborate on the interdependence of the entire region on each of the mini-dragon economies. Since we have highlighted only a few of the emerging economies in the region, opportunities exist to expand the base for further study展开更多
This paper examines the relationship between small- and medium-sized enterprises' (SMEs) entrepreneurs' perceptions towards a proper accounting system with the level of accounting staff recruited, preparation of f...This paper examines the relationship between small- and medium-sized enterprises' (SMEs) entrepreneurs' perceptions towards a proper accounting system with the level of accounting staff recruited, preparation of final accounts, meetings with accounting staff, and entrepreneurs' ranking of accounting according to their priority. The methodology used to gather the data is survey questionnaire. It has been distributed to SMEs based on simple random sampling method. Three hundred businesses were selected from the total of 3,390 businesses listed in Kuala Lumpur, Malaysia area obtained from Small and Medium Industries Development Corporation (SMIDEC) website and a few random selected from Ampang and Cheras areas. Regression analysis has been used to analyze the data. The results of the study reveal that there was a significant relation between SMEs entrepreneurs' perceptions on the importance of a good accounting system and the level of professional accounting staff recruited. Furthermore, findings of this study also found that there was an insignificant relationship between SMEs entrepreneurs' perceptions on the importance of a good accounting system and preparation of final accounts of the company and frequency of meetings with accounting staff.展开更多
Socially-responsible business and ethical behavior of companies have been of interest to academia and practice for decades, but the focus has almost exclusively been on large corporations, while small and medium-sized...Socially-responsible business and ethical behavior of companies have been of interest to academia and practice for decades, but the focus has almost exclusively been on large corporations, while small and medium-sized enterprises (SMEs) have not received as much attention (Hammann, et al., 2009). Worthington, et al (2006) recognized the need to encourage socially-responsible business practices among the 90 per cent or more of the world's businesses that are classified as SMEs. Perrini, et al (2007) found that size explains the differences in firms' willingness to define and implement such specific corporate social responsibility (CSR) strategies, while Murillo and Lozano (2006) concluded that it still remains to be explored whether manufacturing companies tend to apply CSR more extensively, or whether businesses oriented to other business have a tendency to incorporate CSR into their management patterns to a higher degree. Moreover, President Gloria Macapagal-Arroyo, in her 7 June, 2002 speech, recognized the importance of Chinese-Filipinos in helping improve the Philippine economy, and yet, not much research had been done on this sub-sector. Thus, this paper focuses on the effect of finn size and finn activities on the social responsibility practices of selected 30 micro, small and medium-sized enterprises (MSMEs) in Metro Manila which are owned by Chinese-Filipinos. Survey questionnaires and interview were used for gathering data, while a correlations test was used to analyze the data and to conclude whether finn size and finn activities have an effect on the sampled companies' CSR practices.展开更多
基金Supported by Guangxi Science Foundation (0542025)~~
文摘[ Objective] To clone and analyze the sequence of Adiponectin receptor 1 ( AdipoR1 ) and receptor 2 (AdipoR2) cDNA of Guangxi Bama mini-pig. [Method] The Adiponectin receptors cDNAs were amplified by RT-PCR using skeletal muscle total RNA as template and then ligated into pMD18-T vector after purification. The recombinant pMD18-T vector was transformed into the E. coil DH5α for identification and sequencing. And the results were compared with the cDNA sequence from other species. [Result] The fragments, 1 128 bp and 1 161 bp in size, were amplified by RT-PCR and respectively consistent with the coding sequence of AdipoR1 gene and AdipoR2 gene. The homology analysis showed that the sequences of AdipoR1 gene and AdipoR2 gene were respectively 99.8% and 99.7% homologous to the sequence of domestic pig reported in GenBank with one base and three base missense mutations correspondingly. [ Conclusion] The AdipoR1 gene and AdipoR2. gene were successfully amplified from Guangxi Bama mini-pig, laying the foundation for the further study of the biological function of AdipoR genes and the design of novel drugs with AdipoR as target.
基金Supported by Grants from Science and Technology Department of Zhejiang Province, No. 2009C33027 and No. 2009R50040
文摘AIM: To introduce and evaluate a new method to re- pair bile duct defect with a degradable stent and au- tologous tissues. METHODS: Eight Ba-Ma mini-pigs were used in this study, Experimental models with common bile duct (CBD) defect (0,5-1,0 cm segment of CBD resected) were established and then CBD was reconstructed by duct to duct anastomosis with a novel degradable stent made of poly [sebacic acid-co-(1,3-propanediol)-co- (1,2-propanediol)]. In addition, a vascularized greater omentum was placed around the stent and both ends of CBD. Cholangiography via gall bladder was per- formed for each pig at postoperative months 1 and 3 to rule out stent translocation and bile duct stricture. Complete blood count was examined pre- and post- operatively to estimate the inflammatory reaction. Liver enzymes and serum bilirubin were examined pre- and post-operatively to evaluate the liver function. Five pigs were sacrificed at month 3 to evaluate the healing of anastomosis. The other three pigs were raised for one year for long-term observation. RESULTS: All the animals underwent surgery success- fully. There was no intraoperative mortality and no bile leakage during the observation period, The white blood cell counts were only slightly increased on day 14 and month 3 postoperatively compared with that before operation, the difference was not statistically significant (P = 0.652). The plasma level of alanine aminotrans- ferase on day 14 and month 3 postoperatively was also not significantly elevated compared with that before operation (P = 0.810). Nevertheless, the plasma level of y-glutamyl transferase was increased after opera- tion in both groups (P = 0.004), especially 2 wk after operation. The level of serum total bilirubin after opera- tion was not significantly elevated compared with that before operation (P = 0.227), so did the serum direct bilirubin (P = 0.759). By cholangiography yia gall blad- der, we found that the stent maintained its integrity of shape and was stillin situ at month 1, and it disap- peared completely at month 3. No severe CBD dilation and stricture were observed at both months 1 and 3. No pig died during the 3-too postoperative observation period. No sign of necrosis, bile duct stricture, bile leak- age or abdominal abscess was found at reoperation at month 3 postoperatively. Pigs had neither fragments of stent nor stones formed in the CBD. Collagen deposit was observed in the anastomosis by hematoxylin and eosin (HE) and Masson's trichrome stains. No severe cholestasis was observed in liver parenchyma by HE staining. Intestinal obstruction was found in a pig 4 mo after operation, and no bile leakage, bile duct stricture or biliary obstruction were observed in laparotomy. No sign of bile duct stricture or bile leakage was observed in the other two pigs. CONCLUSION: The novel method for repairing bile duct defect yielded a good short-term effect without postoperative bile duct stricture. However, the long- term effect should be further studied.
基金National Natural Science Foundation of China(No.71961016)Planning Fund for the Humanities and Social Sciences of the Ministry of Education(Nos.15XJAZH002,18YJAZH148)Natural Science Foundation of Gansu Province(No.18JR3RA125)。
文摘To eliminate the grey bias and improve ant-jamming performance of the standard grey-Markov forecasting model,a forecasting model based on wavelet packet decomposition and fuzzy grey Markov(FG-Markov)is proposed considering the characteristics of randomness and nonlinearility of freight volume forecasting.Firstly,based on the data analysis ability of wavelet packet to non-stationary random signal,wavelet packet decomposition is used to improve the analysis ability of data signal by decomposing historical freight volume data into wavelet packet component.On this basis,FG-Markov chain is proposed to obtain the transfer probability matrix of wavelet packet coefficients by introducing fuzzy grey variables,and forecast the freight volume by reconstructing wavelet packet coefficients.Finally,an example of Lanzhou railroad hub is carried out in order to testify the validity and applicability of this forecasting model.Compared with neural network model and other forecasting models,the proposed forecasting model can improve the forecasting accuracy under the same conditions.The forecasting accuracy of wavelet packet decomposition and FG-Markov is not only greater than that of any other single forecasting models,but also superior to that of other traditional combinational forecasting models,which can meet the actual requirements of freight volume forecasting.
基金the National Natural Sciences Foundation of China (No.30770664)a grant from Anhui Educational Committee(No. ZD2008008-2)
文摘Objective Machado-Joseph disease (MJD), also known as spinocerebellar ataxia type 3 (SCA3), is a dominant neurodegenerative disorder caused by an expansion of the polyglutamine (polyQ) tract in MJD-1 gene product, ataxin-3 (AT3). This disease is characterized by the formation of intraneuronal inclusions, but the mechanism underlying their formation is still poorly understood. The present study is to explore the relationship between wild type (WT) AT3 and polyQ expanded AT3. Methods Mouse neuroblastoma (N2a) cells or HEK293 cells were co-transfected with WTAT3 and different truncated forms of expanded AT3. The expressions of WT AT3 and the truncated forms of expanded AT3 were detected by Western blotting, and observed by an inverted fluorescent microscope. The interactions between AT3 and different truncated forms of expanded AT3 were detected by immunoprecipitation and GST pull-down assays. Results Using fluorescent microscope, we observed that the truncated forms of expanded AT3 aggregate in transfected cells, and the full-length WT AT3 is recruited onto the aggregates. However, no aggregates were observed in cells transfected with the truncated forms of WT AT3. Immunoprecipitation and GST pull-down analyses indicate that WT AT3 interacts with the truncated AT3 in a polyQ length-dependent manner. Conclusion WT AT3 deposits in the aggregation that was formed by polyQ expanded AT3, which suggests that the formation of AT3 aggregation may affect the normal function of WT AT3 and increase polyQ protein toxicity in MJD.
文摘Two hundred and eighty six lines were selected randomly from the tetraploid wheat (Tritieum turgidum L.var Durum) landrace "Safra Ma'an". The whole population with three check cultivars (ACSAD 65, HOURANI 27 and AMRA) was evaluated at Maru Agriculture Research Station for 16 characters including grain yield per plant. The objectives were to assess the magnitude of phenotypic variations for the traits in "Safra Ma'an" and to evaluate the potential usefulness of some of the traits identified. Results showed wide range of phenotypic variation for several traits. Monomorphism was common for juvenile growth habit, whereas, the rest of the characters exhibited polymorphism in varying degrees. Considering all characters, the average diversity (H') for "Safra Ma'an" landrace was 0.65 ± 0.047. Ten lines were superior to the check (HOURANI 27) for grain yield per plant. Subsequently, the population lines were clustered into six distinct groups at a distance of about 0.55 based on their similarity for all traits, ACSAD 65 and AMRA located in separate clusters, whereas, HOURANI 27 cultivar was presented in cluster with the most lines of "Safra Ma'an". Thirteen lines from the population showed a bluish green cast or glaucousness characters. Glaucous lines had greater kernels per spike. In contrast, this character showed no significant association with grain yield per plant despite the greater grain yield per plant obtained for the glaucous lines. The results are important for the breeding and selection in this land race.
文摘Entrepreneurship and growth are driving forces in the development of emerging economic systems. In this exploratory study we discuss emerging economies within a South-East Asia context. In particular, we expand on a previous study on the East Asian Tiger economies, and introduce the mini-dragon economies of Indonesia, Malaysia, Thailand, and Philippines. We explore the role of various economic indicators and indices entrepreneurship and SMEs as drivers of growth in these emerging economies. Growth factors unique to the region are also identified, and institutional and government interventions are recommended. Furthermore, we elaborate on the interdependence of the entire region on each of the mini-dragon economies. Since we have highlighted only a few of the emerging economies in the region, opportunities exist to expand the base for further study
文摘This paper examines the relationship between small- and medium-sized enterprises' (SMEs) entrepreneurs' perceptions towards a proper accounting system with the level of accounting staff recruited, preparation of final accounts, meetings with accounting staff, and entrepreneurs' ranking of accounting according to their priority. The methodology used to gather the data is survey questionnaire. It has been distributed to SMEs based on simple random sampling method. Three hundred businesses were selected from the total of 3,390 businesses listed in Kuala Lumpur, Malaysia area obtained from Small and Medium Industries Development Corporation (SMIDEC) website and a few random selected from Ampang and Cheras areas. Regression analysis has been used to analyze the data. The results of the study reveal that there was a significant relation between SMEs entrepreneurs' perceptions on the importance of a good accounting system and the level of professional accounting staff recruited. Furthermore, findings of this study also found that there was an insignificant relationship between SMEs entrepreneurs' perceptions on the importance of a good accounting system and preparation of final accounts of the company and frequency of meetings with accounting staff.
文摘Socially-responsible business and ethical behavior of companies have been of interest to academia and practice for decades, but the focus has almost exclusively been on large corporations, while small and medium-sized enterprises (SMEs) have not received as much attention (Hammann, et al., 2009). Worthington, et al (2006) recognized the need to encourage socially-responsible business practices among the 90 per cent or more of the world's businesses that are classified as SMEs. Perrini, et al (2007) found that size explains the differences in firms' willingness to define and implement such specific corporate social responsibility (CSR) strategies, while Murillo and Lozano (2006) concluded that it still remains to be explored whether manufacturing companies tend to apply CSR more extensively, or whether businesses oriented to other business have a tendency to incorporate CSR into their management patterns to a higher degree. Moreover, President Gloria Macapagal-Arroyo, in her 7 June, 2002 speech, recognized the importance of Chinese-Filipinos in helping improve the Philippine economy, and yet, not much research had been done on this sub-sector. Thus, this paper focuses on the effect of finn size and finn activities on the social responsibility practices of selected 30 micro, small and medium-sized enterprises (MSMEs) in Metro Manila which are owned by Chinese-Filipinos. Survey questionnaires and interview were used for gathering data, while a correlations test was used to analyze the data and to conclude whether finn size and finn activities have an effect on the sampled companies' CSR practices.