“吃货”小小白

长长的耳朵，短短的尾巴，红宝石般的眼睛，还有雪白雪白的绒毛，你猜猜它是谁？它就是我心爱的小伙伴——一只可爱的小白兔，它的名字叫小小白。小小白就是一个“吃货”。每天早上一睁开眼，它就蹦蹦跳跳直奔院子角落的菜地，一找到最喜欢吃的大白菜，就开始吃起来。别看它个儿小。

小小白玉兰

一株小小白玉兰,静静地伫立于红楼之外.与周围高大的国槐相比,她确乎太低矮了一些.光润的躯干,较之久经历练的伟岸大树,显得是那样的稚嫩,柔弱.每当劲风吹过,大树挺立,而小小白玉兰已是东摇西曳.但她并不屈服,奋然抗争,用力高昂着纤弱的头.就这样,在大地的滋养下,凭借顽强的毅力,壮大着自己,渐渐地,她已出落成亭亭玉立的少女,以她特有的冰清玉洁,博得了人们的喜爱.

带着“小小白”去旅行

作为一个忠实的C家发烧友和旅行爱好者,我一直关注着佳能的每一支新镜头,希望为自己的旅行找一个合适的伙伴。最近,佳能最新的EF 70-200mm f／4 L IS USM来到了我的身边,让我的选择有了新的方向。

假如我是朵小小白云

在生活中,有些人认为金钱和生命是最重要的,而我认为大自然才是最重要的.倘若没有自然界的一切,人类该怎样存活?那么,就让我变成自然界中一片具有超能力的云彩吧…………

Allelopathic Effect of Adzuki Bean Root Aqueous Extracts on Four Crops

For choosing suitable summer-planting adzuki bean crop, this experiment adopted the method of indoor petri dish bioassay to study the allelopathy effect of adzuki bean, radish, cabbage and wheat under different concentrations of adzuki bean root aqueous extracts. The result showed that the adzuki bean had a strong inhibition towards its germination rate and germination index. With the increasing concentration of the adzuki bean root aqueous extract, the seed germination rate and germination index of the three kinds of receptor crops presented a decreasing trend. From the root length, spire length, the number of lateral roots, plant height, fresh mass and other morphological indexes of seedlings, it was concluded that with the increase of concentration of adzuki bean root aqueous extract, only wheat pre- sented a promotion effect while the other two kinds of receptor crops showed an inhibition effect, which were not significant at low concentrations. With the increase of concentration of adzuki bean root extracts, the SOD and POD activities of radish and wheat also increased, while the two kinds of enzyme activities of cabbage showed a decreasing trend. The MDA contents in seedlings of radish and wheat decreased, while the MDA content in cabbage increased significantly, which showed that wheat could be used as a rotation crop of adzuki bean, and radish also had a great application potential in adzuki bean crop rotation.

Effect of herbal medicine Juzentaihoto on hepatic and intestinal heat shock gene expression requires intestinal microflora in mouse

AIM： To evaluate the role of intestinal microflora in the effects of multi-herbal medicine on gene expression in the gut and liver. METHODS： The multi-herbal medicine Juzentaihoto （JTX） was administered to five germ-free mice and regular mice for 2 wk. Among the results of the comprehensive gene chip analysis of the intestine and liver, we featured heat shock proteins （HSPs） 70 and 105 because their gene expression changed only in the presence of microflora. Real-time RT-PCR was performed to confirm the expression levels of these HSP genes. To determine whether JTX acts directly on the HSP genes, sodium arsenite （SA） was used to induce the heat shock proteins directly. To examine the change of the intestinal microflora with administration of JTX, the terminal restriction fragment polymorphism （T-RFLP） method was used. To identify the changed bacteria, DNA sequencing was performed.documented by gene chip and real-time RT-PCR, changed with the administration of JTX in the regular mice but not in the germ-free mice. JTX did not suppress the direct induction of the HSPs by SA. T-RFLP suggested that JTX decreased unculturable bacteria and increased Lactobacillus johnsoni. These data suggested that JTX changed the intestinal microflora which, in turn, changed HSP gene expression.CONCLUSION： Intestinal microflora affects multi-herbal product JTX on the gene expression in the gut and liver.

Effects of Waterlogging in Different Growth Stages on the Photosynthesis,Growth,Yield,and Protein Content of Three Wheat Cultivars in Jianghan Plain

To maintain high wheat grain yield in areas where frequent and periodic waterlogging occurs,the effects of waterlogging on the photosynthesis,growth,yield,and protein content of three wheat cultivars,namely Xiangmai55(X55),Jingmai102(J102),and Zhengmai9023(Z9023),in four different growth stages were investigated.Experiments were conducted in specially designed experimental tanks in the middle and lower reaches of the Yangtze River during the 2012-2013 and 2013-2014 wheat growing seasons in China.Results showed that X55 was the most susceptible to waterlogging,followed by J102 and Z9023.Chlorophyll content reduction and leaf senescence,which resulted in decreased green-to-total leaf number ratio,were induced to the greatest extents by waterlogging in booting and flowering stages,followed by milky stage.Meanwhile,chlorophyll content in flag leaf,plant height were significantly decreased by waterlogging in jointing stage but effectively recovered after waterlogging withdrawal,and recovery ability varied among the cultivars.Plant biomass and grain yield were most significantly decreased by waterlogging in booting and flowering stages,followed by milky and jointing stages.Grain protein content was also considerably affected by waterlogging depending on growth stage and cultivars.The decreased grain yield caused by waterlogging was mostly due to the sharp decline in 1 000-grain mass.Waterlogging led to reduced protein yield in all growth stages in three wheat cultivars.Above all,in this experiment,waterlogging decreased grain yield significantly,and waterlogging at booting stage and flowering stage was most serious.Comparing the three cultivars,X55 was most sensitive to waterlogging.

Expression of BMP-7 and its receptors in renal tubolo-interstitial fibrosis induced with unilateral ureteral obstruction in rats

Objective:To study the changes of the expression of bone morphogenetic protein-7(BMP-7) and its receptors(BMPR-Ⅱ,ALK2,ALK3 and ALK6) in the renal tubulointerstitial fibrosis induced with unilateral ureteral obstruction in rats. Methods: Sixty Wistar male rats were divided randomly into the normal control,sham-operation and unilateral ureteral obstruction(UUO) groups and the rats were killed on the 1st,3rd,7th and 14th postoperative days respectively.The mRNA level of BMP-7,BMPR-Ⅱ,ALK2,ALK3 and ALK6 was determined with RT-PCR.The site and level of protein expression of BMP-7 were observed with immunohistochemical staining. Results: The mRNA level of BMP-7,BMPR-Ⅱ,ALK2 and ALK3 was significantly decreased in the rats of UUO group than in those of the sham-operation group but the mRNA level of ALK6 showed no obvious changes in all the rats.Immunohistochemical staining revealed that the protein of BMP-7 was mainly expressed in the renal tubules and interstitial tissue of the kidneys in normal rats but it was decreased gradually along with the unilateral ureteral obstruction. Conclusion: It is found that the loss of BMP-7 and its receptors including BMPR-Ⅱ,ALK2 and ALK3 occurs in the early phase of renal tubulointerstitial fibrosis before the appearance of other pathological changes in the kidney and may play an important role in the occurrence and progress of renal tubulointerstitial fibrosis.

Effects of Acupuncture and Moxibustion on Immunologic Suppression in Tumor-bearing Mice

Objective：To observe the effects of electroacupuncture （EA） and moxibustion on the immunologic suppression in tumor.Methods：Forty-eight male Balb/c mice were randomly allocated into an EA group,a moxibustion group,a tumor-bearing group,and a normal group,12 mice in each group.After tumor models were made,mice in the EA and moxibustion groups were treated with EA and moxibustion at Dazhui （GV 14） respectively.Survival rate,tumor inhibited rate,spleen index,and thymus gland index were observed after 6 treatments.The proliferation of spleen lymphocyte of mice stimulated by ConA was measured with AlamarBlue method,and the expression of Foxp3 mRNA,Stat5a mRNA,and Stat5b mRNA of spleen lymphocyte,and Foxp3 mRNA thymus gland lymphocyte were tested with RT-PCR.Results：The survival rate of mice in the moxibustion group was higher than that in the tumor-bearing group without statistic difference.Spleen indexes of mice in the EA group and moxibustion group was higher than that in the tumor-bearing group （P0.05）.Thymus gland indexes of mice in the EA group and moxibustion group were higher than that in the tumor-bearing group without statistic difference.Reducing values of proliferation of spleen lymphocyte of mice in the EA,moxibustion,and tumor-bearing groups were higher than that in the normal group （P0.05）,and the value in the moxibustion group was higher than that in the tumor-bearing group （P0.05）.Expression of Foxp3 mRNA,Stat5a mRNA,and Stat5b mRNA of spleen lymphocyte were up-regulated in the tumor-bearing mice higher than that in the normal mice （P0.05）.The expressions of Foxp3 mRNA,Stat5a mRNA and Stat5b mRNA of the spleen lymphocyte were down-regulated in the EA and moxibustion groups more than that in the tumor-bearing group （P0.05）;the expression of Foxp3 mRNA was higher in thymus gland of tumor-bearing mice than that of the normal mice （P0.05）.Expression of Foxp3 mRNA in thymus gland of mice in the moxibustion group is lower than that in the tumor-bearing group （P0.05）.Expression of Foxp3 mRNA in thymus gland of mice in the EA group was lower than that in the tumor-bearing group without statistic difference.Conclusion：EA and moxibustion could enhance the spleen index and the proliferation of spleen lymphocyte of tumor-bearing mice.The effect of acupuncture-moxibustion therapy on tumor immunologic suppression may be related to the down-regulation of expression of Foxp3 mRNA,Stat5a mRNA,and Stat5b mRNA of the spleen lymphocyte,and Foxp3 mRNA thymus gland lymphocyte.