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舟山市小干二桥工程海洋水动力环境影响分析
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作者 虞羽天 《科技创新与应用》 2023年第13期95-98,共4页
为了解舟山市小干二桥建设工程对所在海域的水动力环境影响,包括工程附近海域的潮流场和泥沙场的变化。该文通过建立平面二维潮流数值模型对小干二桥的潮流场以及水动力进行分析,并对该工程区域的泥沙环境进行冲淤情况预测。结果显示,... 为了解舟山市小干二桥建设工程对所在海域的水动力环境影响,包括工程附近海域的潮流场和泥沙场的变化。该文通过建立平面二维潮流数值模型对小干二桥的潮流场以及水动力进行分析,并对该工程区域的泥沙环境进行冲淤情况预测。结果显示,工程实施后,没有改变大范围海域的流场特征,对周边水域流场的影响主要发生在工程区附近,桥墩桩基附近流向变化较大,桥墩附近流速减弱、流向改变明显,冲淤影响仅限于工程区域附近海域,对沈家门港区和定海至沈家门航道的正常运行均不会造成大的影响。 展开更多
关键词 小干二桥 海洋环境 潮流场 水动力 冲淤变化
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小干扰RNA与冠状动脉粥样硬化性心脏病的关系研究进展 被引量:1
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作者 王宽 王东 +3 位作者 郑柳颖 左国兴 张明惠 杜新平 《山东医药》 CAS 2013年第26期98-100,共3页
小干扰RNA(microRNA)是新近发现的一类由内源基因编码的长度为21~25个核苷酸的非编码单链RNA分子,它参与人体多种生理病理功能。近年来研究发现microRNA与冠状动脉粥样硬化性心脏病(CAHD)的发生、发展与预后存在密切关系。因此,本... 小干扰RNA(microRNA)是新近发现的一类由内源基因编码的长度为21~25个核苷酸的非编码单链RNA分子,它参与人体多种生理病理功能。近年来研究发现microRNA与冠状动脉粥样硬化性心脏病(CAHD)的发生、发展与预后存在密切关系。因此,本文就microRNA与CAHD的关系的研究进展做一综述。 展开更多
关键词 小干状RNA 冠状动脉硬化性心脏病 内皮细胞
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国家海洋经济战略下的商务岛规划探索——浙江舟山群岛新区小干岛商务区城市设计 被引量:2
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作者 王曙光 《上海城市规划》 2012年第6期66-70,共5页
2011年,在海洋经济新形势下,国务院正式批准设立第四个国家新区,即以海洋经济为主题的新区——舟山群岛新区。小干岛成为新区"四岛一城"战略的载体——我国陆海统筹发展先行区舟山群岛"海上花园城"的示范区。小干... 2011年,在海洋经济新形势下,国务院正式批准设立第四个国家新区,即以海洋经济为主题的新区——舟山群岛新区。小干岛成为新区"四岛一城"战略的载体——我国陆海统筹发展先行区舟山群岛"海上花园城"的示范区。小干岛将承担国家海洋经济重任,与长三角都市圈错位互补,提升和完善舟山群岛功能格局,并发扬海洋文化,营造岛岸生活,成为以海洋金融商务为核心、海洋文化为特色的国际金融商务智慧岛。为落实国家战略,加快舟山群岛新区的规划与建设,舟山市建委和舟山市规划局组织开展小干岛商务区城市设计国际方案征集活动。上海市城市规划设计研究院方案参与了征集活动,其规划实践将为海洋经济引领下的海岛商务区规划模式提供一定示范和借鉴。 展开更多
关键词 舟山群岛新区 小干岛商务区 海洋经济 城市设计
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舟山小干大桥通航研究
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作者 关明芳 施政 《水运工程》 北大核心 2011年第5期107-110,135,共5页
舟山市普陀区小干大桥连接舟山本岛与小干岛,跨越沈家门西港区。沈家门港是我国著名的渔港,为渔船避风、补给、水产集散,客货运输的综合性港口。沈家门西港区航道由小干岛、马峙岛与舟山本岛所围成,两岸码头众多,航行船舶的组成较为复... 舟山市普陀区小干大桥连接舟山本岛与小干岛,跨越沈家门西港区。沈家门港是我国著名的渔港,为渔船避风、补给、水产集散,客货运输的综合性港口。沈家门西港区航道由小干岛、马峙岛与舟山本岛所围成,两岸码头众多,航行船舶的组成较为复杂。在分析舟山小干大桥所在的沈家门西港区海道条件、航道条件、港口情况的基础上,从通航的角度,对拟建的舟山小干大桥选址进行分析、对通航净宽进行计算,并结合桥区航道条件,论证通航净空尺度。 展开更多
关键词 小干大桥 沈家门 桥位方案 中心渔港 净空尺度
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舟山市小干岛附近海域环境质量现状监测与评价
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作者 曹静 林柏 +1 位作者 赵沧海 叶新荣 《科技创新导报》 2020年第27期125-130,共6页
本文根据2012—2019年在舟山市小干岛附近海域的海水水质和海洋沉积物质量调查监测数据,对小干岛附近海域水质和沉积物环境现状和变化趋势情况进行分析和评价。调查结果表明:2012—2019年调查期间,小干岛附近海域海水各监测因子中无机... 本文根据2012—2019年在舟山市小干岛附近海域的海水水质和海洋沉积物质量调查监测数据,对小干岛附近海域水质和沉积物环境现状和变化趋势情况进行分析和评价。调查结果表明:2012—2019年调查期间,小干岛附近海域海水各监测因子中无机氮和活性磷酸盐分别存在超过第四类和第三类海水水质标准的现象,而其他海水水质监测因子均可达到第一类海水水质标准要求。调查海区沉积物环境各监测因子均能够达到第一类海洋沉积物质量标准要求,沉积物环境良好。小干岛附近海域呈现富营养化状态,海水中无机氮和活性磷酸盐的环境承载力不足。 展开更多
关键词 小干 海水水质 海洋沉积物质量 富营养化 海域环境承载力
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舟山市小干隧道进洞口管棚方案的确定和施工
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作者 叶惠红 谢海群 黄芳 《中国水运(下半月)》 2010年第9期197-198,共2页
根据小干隧道进洞口地质情况,为保护生态环境,确保施工万无一失,我们采用管棚法施工,工程顺利结束,并且取得较好的经济和社会效益。
关键词 小干隧道 管棚施工 技术参数 施工方法 控制
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PLCE1调控p53诱导食管癌细胞凋亡的实验研究 被引量:17
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作者 李昀 张军航 +2 位作者 安军 何锦园 黄邵洪 《中国药理学通报》 CAS CSCD 北大核心 2015年第1期82-86,共5页
目的探讨磷脂酶Cε1(PLCE1)基因抑制食管癌细胞凋亡的作用机制。方法选用人食管癌细胞株OE33和CPC作为研究对象。采用qRT-PCR、Western blot法分别检测转染PLCE1 siRNA前、后食管癌细胞OE33和CP-C中PLCE1、p53 mRNA和蛋白水平的表达;甲... 目的探讨磷脂酶Cε1(PLCE1)基因抑制食管癌细胞凋亡的作用机制。方法选用人食管癌细胞株OE33和CPC作为研究对象。采用qRT-PCR、Western blot法分别检测转染PLCE1 siRNA前、后食管癌细胞OE33和CP-C中PLCE1、p53 mRNA和蛋白水平的表达;甲基化特异性PCR重亚硫酸盐法检测p53启动子区甲基化状态;流式细胞仪检测细胞凋亡。结果食管癌细胞株OE33和CP-C均高表达PLCE1,抑制PLCE1表达后食管癌细胞CP-C中p53表达上调并促进p53去甲基化,细胞凋亡明显增加。结论 PLCE1通过促进p53启动子区甲基化抑制p53的表达,从而抑制食管癌细胞凋亡。 展开更多
关键词 食管癌 磷脂酶Cε1 P53 细胞凋亡 小干扰RNA 表观遗传学
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鞘内注射突触后致密蛋白95siRNA对神经病理性疼痛大鼠脊髓CaMK Ⅱα活性的影响 被引量:1
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作者 许力 喻洁 +2 位作者 申乐 李旭 黄宇光 《解剖学报》 CAS CSCD 北大核心 2013年第2期170-175,共6页
目的探讨突触后致密蛋白95(PSD-95)基因沉默对神经病理性疼痛模型大鼠脊髓Ca2+/钙调蛋白依赖的蛋白激酶Ⅱα(CaMKⅡα)表达及活性的影响。方法用化学合成针对大鼠PSD-95基因的siRNA转染神经母细胞瘤/大鼠神经胶质细胞瘤杂交瘤细胞(NG108... 目的探讨突触后致密蛋白95(PSD-95)基因沉默对神经病理性疼痛模型大鼠脊髓Ca2+/钙调蛋白依赖的蛋白激酶Ⅱα(CaMKⅡα)表达及活性的影响。方法用化学合成针对大鼠PSD-95基因的siRNA转染神经母细胞瘤/大鼠神经胶质细胞瘤杂交瘤细胞(NG108-15细胞),并观察干扰效果。选取91只成年SD大鼠随机分为3组:Naive组、假手术组(sham)与坐骨神经慢性缩窄损伤(CCI)手术组。CCI组大鼠进一步被分为4个亚组,分别于CCI术后第5天开始鞘内注射生理盐水(Control组)、转染试剂(Vehicle组)、阴性对照(mmRNA组)和PSD-95基因特异siRNA(siRNA组)。连续给药3d,各组大鼠分别于鞘内给药后第1、3、7天评估机械缩足反射阈值(MWT)的改变,并留取腰4~6脊髓节段标本,以Western blotting方法观察脊髓背角PSD-95、CaMKⅡα蛋白表达水平及活性的变化。结果大鼠PSD-95基因特异的siRNA可以有效地沉默NG108-15细胞中PSD-95基因表达。与生理盐水治疗组相比,鞘内注射PSD-95特异siRNA 3d后,大鼠脊髓背角PSD-95蛋白水平明显降低(P<0.05),CCI大鼠神经病理性疼痛得到明显缓解(P<0.05),同时脊髓背角pThr286CaMKⅡα蛋白水平受到明显抑制(P<0.05),而总CaMKⅡα蛋白水平无明显变化(P>0.05)。结论 PSD-95基因特异的siRNA可被成功导入大鼠中枢神经系统,引起脊髓PSD-95基因沉默,在缓解病理性疼痛的同时可抑制神经损伤导致的脊髓CaMKⅡα磷酸化水平的增加,阻断中枢敏化相关的信号通路。 展开更多
关键词 神经病理性疼痛 突触后致密蛋白95 Ca2+ 钙调蛋白依赖的蛋白激酶Ⅱα 慢性压迫损伤 小干 扰RNA RNA干扰 免疫印迹法 大鼠
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The Response of Different Drought-resistance of Wheat Varieties under Drought Stress and the Regulating Role of Nitric Oxygen 被引量:6
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作者 曲小菲 吕淑敏 +3 位作者 王林华 梁书荣 刘魏魏 赵会杰 《Agricultural Science & Technology》 CAS 2010年第4期30-33,63,共5页
[Objective]The aim was to study the response mechanism of drought stress of wheat varieties in different drought-resistance species,and protect the effect of exogenous NO on oxidative damage and photosynthetic apparat... [Objective]The aim was to study the response mechanism of drought stress of wheat varieties in different drought-resistance species,and protect the effect of exogenous NO on oxidative damage and photosynthetic apparatus of wheat leaves under drought stress.[Method]Using low-resistance Yumai 949 and high-resistance Xiamai 5 as test materials,drought stress was carried out to seedlings in five-leaf stage with 15% PEG-6000,and then NO(0.75 mmol/L SNP,sodium nitroprusside,exogenous NO donor) was used for regulation in drought condition,and antioxidant and photosynthetic activities was determined.Three treatments were set in the experiment.[Result]SOD,CAT and APX activities of high resistance Xiamai 5 were much higher than low resistance Yumai 949,so were MDA and chlorophyll content.And the change range of these physiological indexes of high resistance species was smaller than high-yielding and low resistance species under drought stress.NO increased the adaptation to drought stress of these physiological indexes significantly.[Conclusion]Exogenous NO could increase the activity of antioxidant enzymes of wheat leaves under drought stress,and enhance the drought resistance of wheat. 展开更多
关键词 WHEAT Drought stress NO REGULATION
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Study on Change Laws of Cold Tolerance in Wheat under Drought Stress 被引量:2
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作者 曹文昕 万映秀 +2 位作者 张琪琪 李炎 张平治 《Agricultural Science & Technology》 CAS 2016年第8期1862-1865,1932,共5页
In order to study the change laws of cold tolerance in wheat under drought stress, 11 wheat varieties planted in Huang-Huai wheat area and one variety representative in middle-lower Yangtze River wheat area were selec... In order to study the change laws of cold tolerance in wheat under drought stress, 11 wheat varieties planted in Huang-Huai wheat area and one variety representative in middle-lower Yangtze River wheat area were selected, and the their change laws of cold tolerance under normal moisture condition and drought stress condition. The results showed that under drought condition, the cold tolerance of wheat in various growth stages was remarkably lower than that under nondrought condition, and the decreasing amplitudes were the largest in overwintering stage with an average of 4.91 ℃, the smallest in regreening stage, and in the middle in the jointing stage. Under non-drought condition, the cold tolerance of semi- winter varieties in various growth stages was better than that of spring varieties, there were significant differences between different varieties, and the cold tolerance of all varieties in overwintering stage was improved compared with before winter, and rapidly decreased after overwintering. Semiwinter varieties showed cold tolerance remarkably enhanced in overwintering stage, and the cold tolerance of spring varieties was also improved to a certain degree. 展开更多
关键词 WHEAT Drought stress Cold tolerance
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The Division Method of Winter Wheat Drought Risk Zones in Hebei-Shandong-Henan under Irrigation Conditions 被引量:4
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作者 张文宗 张超 +1 位作者 赵春雷 刘晶淼 《Agricultural Science & Technology》 CAS 2010年第3期123-128,共6页
The average rainfall in whole growth period of winter wheat to plus or minus several times of sample variance was used to define different grades of drought.The reduction rate of production in meteorological which was... The average rainfall in whole growth period of winter wheat to plus or minus several times of sample variance was used to define different grades of drought.The reduction rate of production in meteorological which was equal to or greater than the rate of 3% was taken as standards to define wheat drought disaster years.The average reduction ratio of production in the wheat drought years in Hebei-Shandong-Henan was calculated.The frequency distribution rule of wheat in different drought disaster strength of winter wheat in Hebei-Shandong-Henan was analyzed.According to the conception and calculation method of drought disaster risk index of winter wheat,the regional distribution law of drought disaster risk index of winter wheat was analyzed.The division technical methods of winter wheat drought disaster risk zones in Hebei-Shandong-Henan under the irrigated conditions were put forward.Taking the average reduction rate of production in drought years,drought disaster risk index and precipitation of winter wheat in growth periods as indices,using the statistical analysis and overlay function of GIS,the production reduction risk of winter wheat caused by drought disaster in winter wheat zones of Hebei-Shandong-Henan was divided and evaluated.The risk evaluation of production reduction of winter wheat in different risk zones under different climate conditions was realized.The disaster prevention and mitigation measures of winter wheat drought were given. 展开更多
关键词 Hebei-Shandong-Henan Winter wheat drought Risk assessment and division
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Effects of Seaweed Bio-organic Fertilizer on Growth and Yield of Winter Wheat 被引量:5
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作者 徐文文 刘恒洋 +3 位作者 谭秀山 王建明 李明潭 毕建杰 《Agricultural Science & Technology》 CAS 2016年第11期2555-2559,共5页
[Objective] This study aimed to investigate the effects of seaweed bio-or- ganic fertilizer on yield and quality of winter wheat. [Method] Seaweed bio-organic fertilizer was applied to leaves of winter wheat according... [Objective] This study aimed to investigate the effects of seaweed bio-or- ganic fertilizer on yield and quality of winter wheat. [Method] Seaweed bio-organic fertilizer was applied to leaves of winter wheat according to the dose of 45 kg/hm^2 from jointing stage to maturing stage, and plant height, dry matter accumulation, flag leaf photosynthetic characteristics and grain yield of winter wheat were investigated. [Result] Foliar spraying of seaweed bio-organic fertilizer showed little effect on plant height of winter wheat, thickened stems, promoted dry matter accumulation, in- creased flag leaf photosynthetic rate by 3.16%, and increased yield of winter wheat by 6.85%. [Conclusion] Foliar spraying of seaweed bio-organic fertilizer promoted the intelligent growth, thickened the stems, improved the lodging resistance, significantly increased the panicle weight per plant, and increased the bulk density of winter wheat, as well as improving the physical quality of wheat grain. In addition, foliar spraying of seaweed bio-organic fertilizer promoted the synthesis of chlorophyll and mitigated the decomposition of chlorophyll in winter wheat. Under the background of fertilizer-pesticide double reduction, the test results and data of this study can be promoted in the wheat-growing areas of Shandong Province and even whole China. 展开更多
关键词 Seaweed bio-organic fertilizer Winter wheat Dry matter accumulation YIELD Photosynthetic rate
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Chlorella Resistance to Dehydration Conditions and Protection Effect of Different Cryoprotecants on Chlorella Cells 被引量:1
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作者 李会 李环 韦萍 《Agricultural Science & Technology》 CAS 2010年第9期29-31,174,共4页
[Objective] The aim was to study on the chlorella resistance to dehydration conditions and effect of different cryoprotecants on drying of chlorella cells.[Method]The protection effect of seven kinds of cryoprotecants... [Objective] The aim was to study on the chlorella resistance to dehydration conditions and effect of different cryoprotecants on drying of chlorella cells.[Method]The protection effect of seven kinds of cryoprotecants on chlorella cells was analyzed.[Result]The protection effect of trehalose on chlorella cells was the best,and the survival rate of chlorella cells had improved by 50% with the applied trehalose concentration of 5%.[Conclusion]The result in this study had important guidance effect on the mutation breeding of chlorella. 展开更多
关键词 CHLORELLA Dehydrate Cryoprotecant TREHALOSE
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抑制TUBA1C的过表达在卵巢癌细胞株CAOV3、SKOV3细胞增殖侵袭中的作用机制探讨 被引量:2
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作者 江若安 叶枫 王新宇 《浙江医学》 CAS 2014年第8期644-647,共4页
目的探讨抑制α-微管蛋白特异性1C链(TUBA1C)过表达对卵巢癌细胞株CAOV3、SKOV3细胞增殖、侵袭的影响,初步阐明TUBA1C在上皮性卵巢癌中的作用机制。方法通过脂质体介导抑制TUBA1C基因的过表达质粒转染CAOV3、SKOV3细胞,CAOV3分为3组:CA... 目的探讨抑制α-微管蛋白特异性1C链(TUBA1C)过表达对卵巢癌细胞株CAOV3、SKOV3细胞增殖、侵袭的影响,初步阐明TUBA1C在上皮性卵巢癌中的作用机制。方法通过脂质体介导抑制TUBA1C基因的过表达质粒转染CAOV3、SKOV3细胞,CAOV3分为3组:CAOV3实验组、CAOV3空载体转染组和CAOV3空白对照组;SKOV3分为3组:SKOV3实验组、SKOV3空载体转染组和SKOV3空白对照组。采用RT-PCR法检测TUBA1C表达;CCK-8法及Transwell小室体外侵袭实验评价TUBA1C基因抑制后对卵巢癌细胞增殖、侵袭的影响。结果靶向TUBA1C基因的siRNA明显、特异性地抑制TUBA1C mRNA的表达水平;培养72h CAOV3实验组细胞增殖能力较空载体转染组和空白对照组明显降低,差异有统计学意义(P<0.05);侵袭实验结果提示SKOV3实验组穿膜细胞数量为(0.95±0.12)个/视野,较空载体转染组降低,差异有统计学意义(P<0.01),CAOV3实验组穿膜细胞数量为(1.13±0.14)个/视野,较空白对照组降低.但差异无统计学意义(P>0.05)。结论抑制TUBA1C基因过表达可抑制CAOV3、SKOV3细胞的增殖、侵袭能力,有望成为卵巢癌治疗的新靶点。 展开更多
关键词 卵巢肿瘤 细胞增殖 肿瘤侵袭
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Response of Growth and Water Use Efficiency of Spring Wheat to Whole Season CO_2 Enrichment and Drought 被引量:9
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作者 吴冬秀 王根轩 +2 位作者 白永飞 廖建雄 任红旭 《Acta Botanica Sinica》 CSCD 2002年第12期1477-1483,共7页
Whole_growing season pot experiments were conducted to examine the response of growth and water use efficiency ( WUE ) of spring wheat ( Triticum aestivum L. cv. Gaoyuan 602) to CO 2 enrichment. Wheat plants wer... Whole_growing season pot experiments were conducted to examine the response of growth and water use efficiency ( WUE ) of spring wheat ( Triticum aestivum L. cv. Gaoyuan 602) to CO 2 enrichment. Wheat plants were grown in open_top chambers (OTCs) subject to two concentrations of CO 2 ()(350 and 700 μL/L, hereafter 'ambient' and 'elevated' respectively) and three soil water levels (80%, 60% and 40% field water capacity ( FWC ), hereafter 'high soil moisture', 'medium soil moisture' and 'low soil moisture' respectively). Elevated CO 2 greatly increased leaf net photosynthesis ( Pn ) at all three soil water levels. The Pn of plants growing under elevated was 22% lower than that of plants growing at ambient when measured with the same (700 μL/L). Plant growth was enhanced by elevated throughout the growing season, with an increase of 14.8% in shoot dry weight at harvest under high soil moisture, and leaf area was increased by about 20% at all three soil water levels. Elevated in combination with high soil moisture increased the ratio of plant shoot dry weight to height by 15.7%, while this ratio was decreased by over 50% when plants were subject to drought. Elevated also increased the water use efficiency of wheat, mainly due to decreases in transpiration and cumulative consumption of water, and an increase in shoot dry weight, with the biggest value of 30% occurring at high soil water moisture level. Compared to high soil moisture, drought decreased shoot dry weight by 72% under ambient , and by 76% under elevated . Similarly, drought also reduced WUE by 19% under ambient , and 23% under elevated . Our results indicate that: (1) elevated can increase the photosynthetic rates, growth and WUE of wheat plants; (2) long_term exposure to high may result in lower photosynthetic capacity; (3) high stimulates plants lateral growth more than vertical growth; (4) the effects of CO 2 enrichment on plants depend on soil water status, with plants benefiting more from CO 2 enrichment if sufficient water is supplied; and (5) drought may cause relatively more reduction in plant growth and WUE under future elevated conditions. 展开更多
关键词 CO 2 open_top chamber water use efficiency Triticum aestivum DROUGHT
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siRNA对预分化骨髓间充质干细胞β_2M表达的影响
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作者 戴兵 范时洋 +3 位作者 陈龙 金海东 蔡建武 潘骏 《中国病理生理杂志》 CAS CSCD 北大核心 2014年第8期1410-1414,共5页
目的:研究小干扰RNA(siRNA)对预分化骨髓间充质干细胞(BMSCs)β2-微球蛋白(β2M)基因表达的影响。方法:BMSCs成软骨诱导液预分化21 d后,将β2M siRNA用Lipofectamine 2000转染BMSCs、分为转染组、空白对照组和阴性对照组,应用实时定量PC... 目的:研究小干扰RNA(siRNA)对预分化骨髓间充质干细胞(BMSCs)β2-微球蛋白(β2M)基因表达的影响。方法:BMSCs成软骨诱导液预分化21 d后,将β2M siRNA用Lipofectamine 2000转染BMSCs、分为转染组、空白对照组和阴性对照组,应用实时定量PCR、Western blotting、激光共聚焦显微观察等方法检测siRNA对预分化BMSCs的β2M mRNA和蛋白的表达情况,甲苯胺蓝和Ⅱ型胶原免疫荧光检测预分化BMSC的蛋白聚糖多聚体和Ⅱ型胶原分泌情况。结果:实时定量PCR、Western blotting和激光共聚焦显微观察结果显示siRNA成功抑制β2M mRNA和蛋白的表达,甲苯胺蓝和Ⅱ型胶原免疫荧光结果显示siRNA不影响预分化BMSCs蛋白聚糖多聚体和Ⅱ型胶原蛋白的分泌。结论:RNAi干扰β2M可降低BMSCs中β2M mRNA和蛋白的表达,但不影响预分化BMSCs的软骨细胞特性。 展开更多
关键词 Β2-微球蛋白 骨髓间充质干细胞
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Effects of Aphelenchoides besseyi Infection on Yield Traits of Different Rice Varieties (Lines) 被引量:1
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作者 朱镇 于新 +7 位作者 张亚东 陈涛 赵庆勇 周丽慧 姚姝 赵春芳 赵凌 王才林 《Agricultural Science & Technology》 CAS 2016年第5期1173-1176,1180,共5页
[Objective] This study aimed to reveal the effects of Aphelenchoides besseyi infection on different rice varieties(lines).[Method] By field observation and indoor phenotypic investigation,four conventional japonica ... [Objective] This study aimed to reveal the effects of Aphelenchoides besseyi infection on different rice varieties(lines).[Method] By field observation and indoor phenotypic investigation,four conventional japonica rice varieties(lines) and japonica rice restorer line R161 under natural onset conditions were observed and analyzed.[Result] After being infected by A.besseyi,different rice varieties(lines)exhibited various symptoms.Specifically,Ning 1707,Ning 1818,Zhendao 88 and Nanjing 9108 had withered leaf tips and exhibited the symptoms of "small grains and erect panicles";japonica rice restorer line R161 only had withered leaf tips without symptoms of "small grains and erect panicles",and the withering symptoms occurred in flag leaf tip,whole flag leaf and top second leaf,respectively.After being infected by A.besseyi,all the experimental materials could sprout normally,but their plant height,panicle length,seed-setting rate and 1 000-grain weight were affected to varying degrees.In addition,after being infected by A.besseyi,various symptomatic tissues of R161 exerted different effects on rice yield.Especially,panicles with withered and twisted whole flag leaf were most affected.[Conclusion] This study provided the basis for further exploration of the damages of A.besseyi infection to rice and development of corresponding control measures. 展开更多
关键词 Rice(Oryza sativa L.) Aphelenchoides besseyi Small grains and erect panicles Yield traits
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Effects of Different Irrigation Amounts on Wheat Growth in Arid Oasis Area 被引量:2
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作者 杨永龙 钱莉 +1 位作者 刘明春 刘惠兰 《Agricultural Science & Technology》 CAS 2010年第2期102-105,142,共5页
[Objective] The study was conducted to analyze the effects of different irrigation amount on the growth of wheat in arid oasis area and determine effective use measures and reasonable irrigation indices of water in fa... [Objective] The study was conducted to analyze the effects of different irrigation amount on the growth of wheat in arid oasis area and determine effective use measures and reasonable irrigation indices of water in farmland under arid oasis environment,so as to provide reference for the development and management of water-saving technology.[Method] Spring wheat in different growth periods was irrigated for four times.The leaf area index,dry matter content,1 000-grain weight of wheat in different growth peri... 展开更多
关键词 Arid oasis Irrigation quantity Wheat growth
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Effect of Source-sink Change on Photosynthesis and Yield of Wheat 被引量:2
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作者 陈旭 刘佳音 江晓东 《Agricultural Science & Technology》 CAS 2010年第7期59-61,共3页
[Objective] The paper aims to explore the influences of source sink change of wheat at different densities on wheat yield.[Method] 225 (D1) and 320 plant/m2(D2) were adopted,then cutting flag leaf (L1),the secon... [Objective] The paper aims to explore the influences of source sink change of wheat at different densities on wheat yield.[Method] 225 (D1) and 320 plant/m2(D2) were adopted,then cutting flag leaf (L1),the second last leaf (L2),removing upper part of spike (L3) and controlling (CK) were used to conduct field experiment.[Result] The results showed that both of the two densities of wheat's 1 000-grain weight and grain filling rate after anthesis in a decreasing order of L3〉CK〉L2〉L1,and the grain weight per spike and dry matter amount translated after anthesis turned to be CK〉L2〉L1 〉L3.[Conclusion].Both of leaf-cutting and spikelet removing decreased the grain weight per spike and dry matter amount translated after anthesis.Removing upper part of spike increased wheat's 1 000-grain weight.But the decreasing of the sink and dry matter amount translated contributed to the decreasing of the yield of wheat. 展开更多
关键词 Source-sink change WHEAT YIELD Rate of grouting Dry matter transferred yield
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RNA干扰下调PPP2R5C对T-ALL TCR Vβ亚家族分布和克隆增殖的影响 被引量:2
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作者 徐艳 陈宇 +5 位作者 周玲玲 刘思初 杨力建 陈少华 罗更新 李扬秋 《暨南大学学报(自然科学与医学版)》 CAS CSCD 北大核心 2014年第4期361-366,共6页
目的:基于前期利用靶向PPP2R5C基因的小干扰RNA(PPP2R5C-siRNA)抑制白血病T细胞增殖的结果,进一步了解PPP2R5C-siRNA对T-ALL样本中TCR Vβ亚家族基因谱系及其克隆性增殖情况.方法:利用PPP2R5C-siRNA转染2例初发未治疗T-ALL病人外周血单... 目的:基于前期利用靶向PPP2R5C基因的小干扰RNA(PPP2R5C-siRNA)抑制白血病T细胞增殖的结果,进一步了解PPP2R5C-siRNA对T-ALL样本中TCR Vβ亚家族基因谱系及其克隆性增殖情况.方法:利用PPP2R5C-siRNA转染2例初发未治疗T-ALL病人外周血单个核细胞(PBMC),设无关序列对照组(SC)和空白对照组(NC);利用实时定量PCR检测PPP2R5C基因表达水平.利用RT-PCR扩增各组样本中24个TCR Vβ亚家族基因的互补决定区3(CDR3);阳性产物进一步经基因扫描分析CDR3长度,了解其Vβ亚家族T细胞克隆性.结果:PPP2R5C-siRNA处理后明显下调PBMC中PPP2R5C基因表达水平.2例初发T-ALL外周血中分别可检测到10和17个Vβ亚家族,多数Vβ亚家族T细胞为多克隆性,病例1中Vβ9和Vβ17为寡克隆性,而病例2中,Vβ14和Vβ16呈寡克隆性;经过RNA干扰处理后,TCR Vβ亚家族表达率降低,仅检测到3和4个Vβ亚家族,分别为Vβ2,Vβ3,Vβ16和Vβ17.其中2个Vβ家族的寡克隆性模式发生改变,病例1中,Vβ17转为多克隆,而病例2中,Vβ16也转变为多克隆.结论:RNA干扰下调白血病T细胞PPP2R5C基因表达抑制细胞增殖时,在一定程度上影响TCR Vβ亚家族T细胞增殖和克隆模式变化,对正常T细胞功能可能存在一定影响. 展开更多
关键词 PPP2R5C基因 T细胞-急性淋巴细胞白血病 T细胞受体VΒ基因 T细胞克隆性
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