Objective: To study and compare the hemocompatibility of MWCNTs and hydroxyl modificated MWCNTs (MWCNTs-OH). Methods: MWCNTs and MWCNTs-OH were characterized by scanning electron microscope, Fourier transform infrared...Objective: To study and compare the hemocompatibility of MWCNTs and hydroxyl modificated MWCNTs (MWCNTs-OH). Methods: MWCNTs and MWCNTs-OH were characterized by scanning electron microscope, Fourier transform infrared spectroscopy, water contact angle assays, platelet-adhesion and hemolytic rate assays. Results: The results showed that the two MWCNTs had a similar surface topography and MWCNTs-OH were functionalized with hydroxyl groups on their surfaces. Water contact angle assays indicated that MWCNTs were hydrophobic materials, whereas MWCNTs-OH was hydrophilic. The platelet-adhesion assays displayed that the platelet-adhesion rate of MWCNTs-OH was much lower than MWCNTs. The hemolytic rate assays showed that the hemolytic rates of both MWCNTs were lower than the standard value of 5%. Conclusion: MWCNTs-OH shows superior anticoagulant capacity over MWCNTs. Both MWCNTs and MWCNTs-OH are nonhemolytic materials.展开更多
Porcine deltacoronavirus(PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDC...Porcine deltacoronavirus(PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and China's Mainland. Here we isolated a strain of PDCoV, named CHN-GD-2016,from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1×10~5 TCID_(50) of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong,China and is highly pathogenic in newborn piglets.展开更多
基金National Natural Science Foundation of Chinagrant number:11075116 and 51272176+3 种基金National Basic Research Program of China(973 Program)grant number:2012CB933604Open Research Fund of the State Key Laboratory of Bioelectronics,Southeast Universitythe Key Laboratory of Beam Technology and Material Modification of the Ministry of Education,Beijing Normal University
文摘Objective: To study and compare the hemocompatibility of MWCNTs and hydroxyl modificated MWCNTs (MWCNTs-OH). Methods: MWCNTs and MWCNTs-OH were characterized by scanning electron microscope, Fourier transform infrared spectroscopy, water contact angle assays, platelet-adhesion and hemolytic rate assays. Results: The results showed that the two MWCNTs had a similar surface topography and MWCNTs-OH were functionalized with hydroxyl groups on their surfaces. Water contact angle assays indicated that MWCNTs were hydrophobic materials, whereas MWCNTs-OH was hydrophilic. The platelet-adhesion assays displayed that the platelet-adhesion rate of MWCNTs-OH was much lower than MWCNTs. The hemolytic rate assays showed that the hemolytic rates of both MWCNTs were lower than the standard value of 5%. Conclusion: MWCNTs-OH shows superior anticoagulant capacity over MWCNTs. Both MWCNTs and MWCNTs-OH are nonhemolytic materials.
基金supported by the National Key Research and Development Program (2016YFD0500101)
文摘Porcine deltacoronavirus(PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and China's Mainland. Here we isolated a strain of PDCoV, named CHN-GD-2016,from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1×10~5 TCID_(50) of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong,China and is highly pathogenic in newborn piglets.
