The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7...The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7.2 and 7.4; inoculation time： 0, 24 and 48 h; inoculation dose： 0.10%, 1.00% and 10.00%; harvest time： 48, 72 and 96 h）. The results showed that the optimal conditions were as follows： cell culture medium pH value of 7.2, synchronous inoculation, inoculation dose of 0.10% and culture time of 96 h. Among the four factors, culture time was the most important factor affectina the virulence titer of PPV N strain.展开更多
The bottleneck analysis of the minimum cost problem for the generalized network (MCPGN) is discussed. The analysis is based on the network simplex algorithm, which gains negative cost graphs by constructing augmented ...The bottleneck analysis of the minimum cost problem for the generalized network (MCPGN) is discussed. The analysis is based on the network simplex algorithm, which gains negative cost graphs by constructing augmented forest structure, then augments flows on the negative cost graphs until the optimal revolution is gained. Bottleneck structure is presented after analyzing the augmented forest structure. The negative cost augmented graphs are constructed with the bottleneck structure. The arcs that block the negative cost augmented graph are the elements of the bottleneck. The bottleneck analysis for the generalized circulation problem, the minimum circulation problem and the circulation problem are discussed respectively as the basal problems, then that for MCPGN is achieved. An example is presented at the end.展开更多
基金Supported by Nonprofit Institute Research Grant of Guangxi Province(GK15-2,GK16-2)Guangxi Aquatic Animal Husbandry Science and Technology Project(GYMK201528030,201633034)~~
文摘The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7.2 and 7.4; inoculation time： 0, 24 and 48 h; inoculation dose： 0.10%, 1.00% and 10.00%; harvest time： 48, 72 and 96 h）. The results showed that the optimal conditions were as follows： cell culture medium pH value of 7.2, synchronous inoculation, inoculation dose of 0.10% and culture time of 96 h. Among the four factors, culture time was the most important factor affectina the virulence titer of PPV N strain.
基金Supported by the National Natural Science Foundation of China (No. 60174046).
文摘The bottleneck analysis of the minimum cost problem for the generalized network (MCPGN) is discussed. The analysis is based on the network simplex algorithm, which gains negative cost graphs by constructing augmented forest structure, then augments flows on the negative cost graphs until the optimal revolution is gained. Bottleneck structure is presented after analyzing the augmented forest structure. The negative cost augmented graphs are constructed with the bottleneck structure. The arcs that block the negative cost augmented graph are the elements of the bottleneck. The bottleneck analysis for the generalized circulation problem, the minimum circulation problem and the circulation problem are discussed respectively as the basal problems, then that for MCPGN is achieved. An example is presented at the end.
