山东沂南高油酸花生新品种(小粒组)筛选试验

为了筛选出适宜山东省沂南县种植的品质优、商品性好、产量较高的高油酸花生新品种,为高油酸花生的推广种植和基地建设提供数据支撑和参考,2021年沂南县开展了高油酸花生新品种(小粒组)筛选试验。试验结果表明,参试新品种冀花51、阜花36、漯花107、驻科花12号增产效果显著,且油酸含量均在79%以上,抗性好,综合表现突出,均适宜在沂南县示范推广种植。其中,冀花51油酸含量高达82.2%,荚果产量为448.5 kg/亩,籽仁产量为348.3 kg/亩,花生品质和产量指标均居参试品种第1位,建议作为沂南县高油酸花生(小粒组)推荐品种优先示范推广种植。

Construction and Characterization of a Bacterial Artificial Chromosome Library for Triticum boeoticum

A bacterial artificial chromosome library has been constructed for Triticum boeoticum Boiss (A bA b) using the bacterial artificial chromosome (BAC) vector pECBAC1. The library consists of about 170 000 clones. A random sampling analysis of 200 BAC clones indicates that the average insert size is 104 kb. Based on the genome size of T. boeoticum, the library is about three times as large as T. boeoticum haploid genome (5 600 Mb). Screening the BAC library with cpDNA sequence psbA gene and mtDNA sequence atp6 gene as probe shows that contamination of the library with chloroplast and mitochondrial clones is less than 1%. The library will be a useful platform in gene clone and genomic research of wheat.

Effects of augmentation of liver regeneration recombinant plasmid on rat hepatic fibrosis

AIM: To investigate the effects of eukaryotic expression of plasmid on augmentation of liver regeneration (ALR) in rat hepatic fibrosis and to explore their mechanisms. METHODS: Ten rats were randomly selected from 50 Wistar rats as normal control group. The rest were administered intraperitoneally with porcine serum twice weekly. After 8 wk, they were randomly divided into: model control group, colchicine group (Col), first ALR group (ALR1), second ALR group (ALR2). Then colchicine ALR recombinant plasmid were used to treat them respectively. At the end of the 4th wk, rats were killed. Serum indicators were detected and histopathological changes were graded. Expression of type Ⅰ, Ⅲ, collagen and TIMP-1 were detected by immunohisto-chemistry and expression of TIMP-1 mRNA was detected by semi-quantified RT-PCR. RESULTS: The histologic examination showed that the degree of the rat hepatic fibrosis in two ALR groups was lower than those in model control group. Compared with model group, ALR significantly reduced the serum levels of ALT, AST, HA, LN, PCIII and IV (P<0.05). Immunohistochemical staining showed that expression of type Ⅰ, Ⅲ, collagen and TIMP-1 in two ALR groups was ameliorated dramatically compared with model group (I collagen: 6.94±1.42,5.80±1.66 and 10.83±3.58 in ALR1, ALR2 and model groups, respectively; Ⅲ collagen: 7.18±1.95, 4.50±1.67 and 10.25±2.61, respectively; TIMP-1: 0.39±0.05,0.20±0.06 and 0.53±0.12, respectively,P<0.05 or P<0.01). The expression level of TIMP-1 mRNA in the liver tissues was markedly decreased in two ALR groups compared with model group (TIMP-1 mRNA/β-actin: 0.89±0.08, 0.65±0.11 and 1.36±0.11 in ALR1, ALR2 and model groups respectively, P<0.01). CONCLUSION: ALR recombinant plasmid has beneficial effects on rat hepatic fibrosis by enhancing regeneration of injured liver cells and inhibiting TIMP-1 expressions.

Proteomic analysis of glutathione S-transferase isoforms in mouse liver mitochondria

AIM:To survey glutathione(GSH) S-transferase(GST) isoforms in mitochondria and to reveal the isoforms' biological significance in diabetic mice.METHODS:The presence of GSTs in mouse liver mitochondria was systematically screened by two proteomic approaches,namely,GSH affinity chromatography/two dimensional electrophoresis(2DE/MALDI TOF/TOF MS) and SDS-PAGE/LC ESI MS/MS.The proteomic results were further confirmed by Western blotting using monoclonal antibodies against GSTs.To evaluate the liver mitochondrial GSTs quantitatively,calibration curves were generated by the loading amounts of individual recombinant GST protein vs the relative intensities elicited from the Western blotting.An extensive comparison of the liver mitochondrial GSTs was conducted between normal and db/db diabetic mice.Student's t test was adopted for the estimation of regression and significant difference.RESULTS:Using GSH affinity/2DE/MALDI TOF/TOF MS,three GSTs,namely,alpha3,mu1 and pi1,were identified;whereas five GSTs,alpha3,mu1,pi1,kappa1 and zeta1,were detected in mouse liver mitochondria using SDS-PAGE/LC ESI MS/MS,of these GSTs,GST kappa1 was reported as a specific mitochondrial GST.The R 2 values of regression ranged between values of about 0.86 and 0.98,which were acceptable for the quantification.Based on the measurement of the GST abundances in liver mitochondria of normal and diabetic mice,the four GSTs,alpha3,kappa1,mu1 and zeta1,were found to be almost comparable between the two sets of animals,whereas,lower GST pi1 was detected in the diabetic mice compared with normal ones,the signal of Western blotting in control and db/db diabetic mice liver mitochondria is 134.61 ± 53.84 vs 99.74 ± 46.2,with P < 0.05.CONCLUSION:Our results indicate that GSTs exist widely in mitochondria and its abundances of mitochondrial GSTs might be tissue-dependent and disease-related.

Vaccination with dendritic cells pulsed with hepatitis C pseudo particles induces specific immune responses in mice

AIM: To explore dendritic cells (DCs) multiple functions in immune modulation. METHODS: We used bone-marrow derived dendritic cells from BALB/c mice pulsed with pseudo particles from the hepatitis C virus to vaccinate naive BALB/c mice. Hepatitis C virus (HCV) pseudo particles consist of the genotype 1b derived envelope proteins E1 and E2, covering a non-HCV core structure. Thus, not a single epitope, but the whole "viral surface" induces immunogenicity. For vaccination, mature and activated DC were injected subcutaneously twice. RESULTS: Humoral and cellular immune responses measured by enzyme-linked immunosorbent assay and interferon-gamma enzyme-linked immunosorbent spot test showed antibody production as well as T-cellsdirected against HCV. Furthermore, T-cell responses confi rmed two highly immunogenic regions in E1 and E2 outside the hypervariable region 1. CONCLUSION: Our results indicate dendritic cells as a promising vaccination model for HCV infection that should be evaluated further.

Effect and mechanism of (125)~I radioactive particles interposed radiotherapy between organizations on lung cancer

Objective:The aim of this study was to evaluate effect and mechanism of 125I radioactive particles interposed radiotherapy between organizations on lung cancer.Methods:Fourteen cases of patients diagnosed with non-small cell lung cancer(NSCLC),the use of the B-,CT-guided,according to preoperative imaging and treatment planning system(TPS) program for radioactive particles interposed 125I interstitial radiotherapy.Results:All patients were successfully 125I interstitial radioactive particles interposed radiotherapy.Postoperative local complete tumor remission in 9 cases,partial remission in 5 cases,the efficiency of 100%.No case of serious complications.After 3 to 4 weeks of chemotherapy after 11 cases.4 cases of lung cancer with bone metastases,pain completely disappeared after treatment.Up to now,five cases have died due to tumor progression,survival time of 12 to 16 months.Nine cases still under follow-up observation and treatment.Conclusion:125 I radioactive particles interposed radiotherapy between organizations of lung cancer,simple operation,trauma,fewer complications,conformal high,high local tumor dose,efficacy,and is a supplement of modern radiotherapy techniques for the treatment of lung cancer provides a comprehensive line of the method of effective.

Preparation of Nano-sized Silica-alumina Support by Supersolubilizing Self-assembly Technique

Nano-sized silica-alumina particles were in-situ synthesized in supersolubilizing reverse micellae.Both the most probable pore diameters and the particle sizes were distributed in nano-scale SiO2-Al2O3 particulates.The influence of SiO2/Al2O3 mass ratio and the surfactant content on the particle size and morphology,pore structure,and acidity was characterized by the low temperature nitrogen adsorption/desorption (BET),SEM,TEM and NH3-TPD methods.The test results indicated that the most probable pore diameter of SiO2-Al2O3 nanoparticles was around 10 nm,the specific surface area was about 223-286 m2 /g,the pore volumes were about 0.48-0.63 cm 3 /g,and the particle sizes of porous SiO2-Al2O3 calcined at 550 ℃ were distributed always in the range between 10 nm to 50 nm.The calcined SiO2-Al2O3 nano-powders showed their acidity being stronger than the porous γ-Al2O3 support.

山东沂南2021年花生新品种试验

花生是山东省的重要油料作物、经济和出口创汇作物之一,为了鉴定花生新品种的丰产性、稳产性、适应性、抗逆性、品质等综合性状,客观评价品种特性及生产利用价值,为品种登记提供依据,为新品种示范推广利用提供参考,山东省农业产业体系花生创新团队按照与沂南县花生综合试验站签订的《山东省花生产业技术体系建设任务协议书》,在沂南县苏村镇开展了花生新品种试验。试验分为大粒组和小粒组2个试验组,确定了17A08、山花45号、山花9号(CK)等7个大花生品种和山花34号、济花1010、花育20(CK)等4个小花生品种为参试品种。由田间试验观察记载和室内考种结果可知,在花生新品种多点试验中,大粒组参试品种17A08、菏花1号这2个新品种和小粒组参试品种济花1010、山花34号这2个新品种,比对照品种增产明显,产量高、抗性强、适应性好,综合表现突出,均适宜在沂南县优先推广种植。

Plutella xylostella granulovirus late gene promoter activity in the context of the Autographa californica multiple nucleopolyhedrovirus genome

Within Baculoviridae, little is known about the molecular mechanisms of replication in betabaculoviruses, despite extensive studies in alphabaculoviruses. In this study, the promoters of nine late genes of the betabaculovirus Plutella xylostella granulovirus(Plxy GV) were cloned into a transient expression vector and the alphabaculovirus Autographa californica multiple nucleopolyhedrovirus(Ac MNPV) genome, and compared with homologous late gene promoters of Ac MNPV in Sf9 cells. In transient expression assays, all Plxy GV late promoters were activated in cells transfected with the individual reporter plasmids together with an Ac MNPV bacmid. In infected cells, reporter gene expression levels with the promoters of Plxy GV e18 and Ac MNPV vp39 and gp41 were significantly higher than those of the corresponding Ac MNPV or Plxy GV promoters,which had fewer late promoter motifs. Observed expression levels were lower for the Plxy GV p6.9,pk1, gran, p10 a, and p10 b promoters than for the corresponding Ac MNPV promoters, despite equal numbers of late promoter motifs, indicating that species-specific elements contained in some late promoters were favored by the native viral RNA polymerases for optimal transcription. The 8-nt sequence TAAATAAG encompassing the ATAAG motif was conserved in the Ac MNPV polh, p10,and pk1 promoters. The 5-nt sequence CAATT located 4 or 5 nt upstream of the T/ATAAG motif was conserved in the promoters of Plxy GV gran, p10 c, and pk1. The results of this study demonstrated that Plxy GV late gene promoters could be effectively activated by the RNA polymerase from Ac MNPV, implying that late gene expression systems are regulated by similar mechanisms in alphabaculoviruses and betabaculoviruses.